Kindlins are focal adhesion proteins that regulate integrin activation and outside-in signaling. The kindlin family consists of three members, kindlin-1, -2, and -3. Kindlin-2 is widely expressed in multiple cell types, except those from the hematopoietic lineage. A previous study has reported that the Drosophila Fit1 protein (an ortholog of kindlin-2) prevents abnormal spindle assembly; however, the mechanism remains unknown. Here, we show that kindlin-2 maintains spindle integrity in mitotic human cells. The human neuroblastoma SH-SY5Y cell line expresses only kindlin-2, and we found that when SH-SY5Y cells are depleted of kindlin-2, they exhibit pronounced spindle abnormalities and delayed mitosis. Of note, acetylation of α-tubulin, which maintains microtubule flexibility and stability, was diminished in the kindlin-2–depleted cells. Mechanistically, we found that kindlin-2 maintains α-tubulin acetylation by inhibiting the microtubule-associated deacetylase histone deacetylase 6 (HDAC6) via a signaling pathway involving AKT Ser/Thr kinase (AKT)/glycogen synthase kinase 3β (GSK3β) or paxillin. We also provide evidence that prolonged hypoxia down-regulates kindlin-2 expression, leading to spindle abnormalities not only in the SH-SY5Y cell line, but also cell lines derived from colon and breast tissues. The findings of our study highlight that kindlin-2 regulates mitotic spindle assembly and that this process is perturbed in cancer cells in a hypoxic environment.

This is a simplified version of a traditional Italian spinach and ricotta pie, commonly eaten on Good Friday.

The frittata has almost overtaken the classic quiche. It is a healthier option as it doesn't have the extra kilojoules of a pastry case. It's Italian in origin and a versatile dish as you can serve it hot or at room temperature for lunch or dinner, alone or with a salad or vegetables, as part of an Antipasto selection or cut in bite-size pieces to serve as a nibble with drinks.

This recipe features on Foodie Tuesday, a weekly segment on 774 Drive with Raf Epstein, 3.30PM, courtesy of Tony Tan

Breslau : E. Morgenstern, 1882.

Berlin : A. Hirschwald, 1865.

Leipzig : F. Deuticke, 1908.

Edinburgh : Young J. Pentland, 1884.

Boston : Wright & Potter Printing Co, 1898.

Londres : Paris, 1840.

Matthias Löwe, Kristina Schubert.

Source: Electronic Journal of Statistics, Volume 14, Number 1, 1796--1815.

Abstract:
We show how to exactly reconstruct the block structure at the critical line in the so-called Ising block model. This model was recently re-introduced by Berthet, Rigollet and Srivastava in [2]. There the authors show how to exactly reconstruct blocks away from the critical line and they give an upper and a lower bound on the number of observations one needs; thereby they establish a minimax optimal rate (up to constants). Our technique relies on a combination of their methods with fluctuation results obtained in [20]. The latter are extended to the full critical regime. We find that the number of necessary observations depends on whether the interaction parameter between two blocks is positive or negative: In the first case, there are about $Nlog N$ observations required to exactly recover the block structure, while in the latter case $sqrt{N}log N$ observations suffice.

Holger Sambale, Arthur Sinulis.

Source: Bernoulli, Volume 26, Number 3, 1863--1890.

Abstract:
We derive sufficient conditions for a probability measure on a finite product space (a spin system ) to satisfy a (modified) logarithmic Sobolev inequality. We establish these conditions for various examples, such as the (vertex-weighted) exponential random graph model, the random coloring and the hard-core model with fugacity. This leads to two separate branches of applications. The first branch is given by mixing time estimates of the Glauber dynamics. The proofs do not rely on coupling arguments, but instead use functional inequalities. As a byproduct, this also yields exponential decay of the relative entropy along the Glauber semigroup. Secondly, we investigate the concentration of measure phenomenon (particularly of higher order) for these spin systems. We show the effect of better concentration properties by centering not around the mean, but around a stochastic term in the exponential random graph model. From there, one can deduce a central limit theorem for the number of triangles from the CLT of the edge count. In the Erdős–Rényi model the first-order approximation leads to a quantification and a proof of a central limit theorem for subgraph counts.

Savvas Learning Company will continue to provide its K-12 products and services, and is working to support districts with their remote learning needs during school closures.

The post Pearson K12 Spinoff Rebranded as ‘Savvas Learning Company’ appeared first on Market Brief.

KM Harris
Jul 1, 1992; 12:2685-2705
Articles

KM Harris
Aug 1, 1989; 9:2982-2997
Articles

Structural plasticity of dendritic spines is a key component of the refinement of synaptic connections during learning. Recent studies highlight a novel role for the NMDA receptor (NMDAR), independent of ion flow, in driving spine shrinkage and LTD. Yet little is known about the molecular mechanisms that link conformational changes in the NMDAR to changes in spine size and synaptic strength. Here, using two-photon glutamate uncaging to induce plasticity at individual dendritic spines on hippocampal CA1 neurons from mice and rats of both sexes, we demonstrate that p38 MAPK is generally required downstream of non-ionotropic NMDAR signaling to drive both spine shrinkage and LTD. In a series of pharmacological and molecular genetic experiments, we identify key components of the non-ionotropic NMDAR signaling pathway driving dendritic spine shrinkage, including the interaction between NOS1AP (nitric oxide synthase 1 adaptor protein) and neuronal nitric oxide synthase (nNOS), nNOS enzymatic activity, activation of MK2 (MAPK-activated protein kinase 2) and cofilin, and signaling through CaMKII. Our results represent a large step forward in delineating the molecular mechanisms of non-ionotropic NMDAR signaling that can drive shrinkage and elimination of dendritic spines during synaptic plasticity.

SIGNIFICANCE STATEMENT Signaling through the NMDA receptor (NMDAR) is vitally important for the synaptic plasticity that underlies learning. Recent studies highlight a novel role for the NMDAR, independent of ion flow, in driving synaptic weakening and dendritic spine shrinkage during synaptic plasticity. Here, we delineate several key components of the molecular pathway that links conformational signaling through the NMDAR to dendritic spine shrinkage during synaptic plasticity.

Its paddle-like tail, unearthed in Morocco, suggests the Cretaceous carnivore ventured into the water to hunt

Spinal muscular atrophy with respiratory distress type 1 (SMARD1) is a progressive, inherited neuromuscular disease manifesting with diaphragmatic paralysis in the first year of life. All patients need mechanical ventilation.

We describe the natural course of SMARD1, developed a scoring system, and defined prognostic values. The clinical outcome of the patients was heterogeneous, and residual enzymatic activity of the IGHMBP2 protein was associated with a more benign disease course. (Read the full article)

There is no literature on the concentrations of vitamin B₆ vitamers in cerebrospinal fluid of preterm and term newborn infants. This knowledge, however, is highly important, because vitamin B₆ plays a pivotal role in brain development and functioning.

In cerebrospinal fluid of newborn infants, B₆ vitamer concentrations are strongly dependent on postmenstrual age, indicating that vitamin B₆ homeostasis in brain differs between preterm and term newborns. This has implications for the evaluation of epilepsy and vitamin B₆ deficiency. (Read the full article)

High doses of radiation have been linked to cancer induction in irradiated populations such as atomic bomb survivors. Medical imaging directs significant radiation doses to human tissues. Epidemiological studies have demonstrated that children are more sensitive to radiation than adults.

The link between cancer induction from moderate radiation doses such as diagnostic imaging is controversial. This study uses Food and Drug Administration–accepted formulas to calculate theoretical risk of breast cancer induction in female pediatric trauma patients receiving diagnostic imaging of the thoracic spine. (Read the full article)

Survival of children with spinal muscular atrophy type 1 is determined by treatment choice: tracheostomy with mechanical ventilation, noninvasive mechanical ventilation, or a palliative approach. Few data are available on life expectancies with different approaches.

The present study provides data comparing therapeutic strategies that affect life expectancy. Clinicians involved in the care of patients with spinal muscular atrophy type 1 should be aware of survival trends while awaiting more definitive therapeutic strategies. (Read the full article)

Practice standards for managing adult cervical spine injuries (CSIs) are well established. However, pediatric CSIs are rare and different from those of adults, preventing extrapolation from adult practice and illustrating the need for larger multicenter investigations of CSIs in children.

This study comprehensively describes CSIs in a large multicenter pediatric cohort. The large number of young children included allowed us to comprehensively explore the relationship between CSI pattern and age, mechanism of injury, comorbid injuries, surgical interventions, and neurologic outcome. (Read the full article)

Functional capabilities in patients with spina bifida depend on the spinal level of the lesion and its type. Sociodemographic characteristics have been shown in other conditions to be an important additional influence on outcomes, making them important for risk adjustment.

Males, non-Hispanic blacks, and patients without private insurance have less favorable functional outcomes in spina bifida, and age also has an impact. These attributes need to be considered by clinicians and researchers and used in comparing care outcomes across clinic settings. (Read the full article)

Anidulafungin and micafungin were quantified in cerebrospinal fluid (CSF) of critically ill adults and in cerebral cortex of deceased patients. In CSF, anidulafungin levels (<0.01-0.66 μg/ml) and micafungin levels (<0.01-0.16 μg/ml) were lower than the simultaneous plasma concentrations (0.77-5.07 μg/ml and 1.21-8.70 μg/ml, respectively). In cerebral cortex, anidulafungin and micafungin levels were 0.21-2.34 μg/g and 0.18-2.88 μg/g, respectively. Thus, MIC values of several pathogenic Candida strains exceed concentrations in CSF and in brain.

Linezolid has strong antimicrobial activity against the multidrug-resistant (MDR) strains of Mycobacterium tuberculosis. Little is known about the distribution of linezolid in tuberculosis (TB) lesions in patients with MDR-TB. The aim of this study is to evaluate the distribution of linezolid in TB lesions in patients with spinal MDR-TB. Nine patients with spinal MDR-TB were enrolled prospectively from August 2019 to February 2020. The patients received a linezolid-containing anti-TB treatment regimen and needed surgery for the removal of TB lesions. During the operation, nine blood samples, eight diseased bone tissue samples, seven pus samples and four granulation tissue samples were collected simultaneously and 2 h after the oral administration of 600 mg of linezolid. Linezolid concentrations in plasma, diseased bone tissue, pus, and granulation tissue samples were subjected to high-performance liquid chromatography–tandem mass spectrometry. At sample collection, the mean concentrations of linezolid in plasma, diseased bone tissue, pus, and granulation tissue samples of the nine patients were 11.14 ± 5.82, 5.94 ± 4.27, 11.09 ± 4.58, 14.08 ± 10.61 mg/L, respectively. The mean ratios of linezolid concentration in diseased bone/plasma, pus/plasma, and granulation/plasma were 53.84%, 91.69%, and 103.57%, respectively. The mean ratios of linezolid concentration in pus/plasma and granulation/plasma were higher than those in diseased bone/plasma, and the difference was statistically significant (t =-2.810, p = 0.015; t =-4.901, p = 0.001). In conclusion, linezolid had different concentration distributions in different types of TB infected tissues in patients with spinal MDR-TB.

If you are finding those toasts, butter and half fried eggs a tad too monotonous, you can think of adding this delicious smoothie to the fare.

The unprecedented situation created by Covid–19 pandemic and lockdown announced by both the Union and state governments from March 24 has led to severe disruptions affecting the economy and impacting people at large and the MSME spinning mills.

As the lockdown in China was lifted from the beginning of April 2020 onwards, spinners there have been scaling up operations.

Poonam Yadav (4/19) snapped four wickets, two in successive deliveries of the 12th over to break the backbone of Australia's chase.

Marissa Mayer, chief executive of Yahoo, said on Tuesday that the internet company would spin off its 15.4% stake in Alibaba...

By spinning off stake into a separate firm, Yahoo will be judged by its Web businesses

Sour Cream Chicken, Mushrooms and Spinach appeared first on CASA Veneracion

A simple tasty dish cooked with only nine ingredients (including salt and pepper). Total cook time for this sour cream chicken, mushrooms and spinach dish: 20 minutes. The secret? The freshest ingredients and lot of butter. How much sauce and how thick it should be depends on you. I recommend making the sauce a bit …

Sour Cream Chicken, Mushrooms and Spinach was written by Connie Veneracion and originally published in CASA Veneracion.

Wind River, an IoT and industrial operating system owned by Intel will be acquired by TPG, global alternative asset firm. Terms of the deal were not disclosed. Intel had bought Wind River Systems for $884 million in 2009

Wind River operates in several markets, including aerospace and defense, automotive, industrial, medical and networking technologies. Its core products in these markets are operating systems, software infrastructure platforms, device management, and simulation software. The IoT practice of Wind River provides consulting services for customers building IoT applications.

In a statement for Wind River, Nehal Raj, Partner and Head of Technology investing at TPG said “We see a tremendous market opportunity in industrial software driven by the convergence of the Internet of Things (IoT), intelligent devices and edge computing. As a market leader with a strong product portfolio, Wind River is well positioned to benefit from these trends. We are excited about the prospects for Wind River as an independent company, and plan to build on its strong foundation with investments in both organic and inorganic growth.”

Wind River’s main IoT product is Helix Device Cloud, a cloud-offering capable of managing deployed IoT devices and industrial equipment across a machine’s lifecycle. Helix can connect and manage devices remotely.

Helix platform’s key uses cases are gateway management, proactive maintenance, security updates, and device provisioning.

It’s been a whirlwind year for Packetized Energy, the Vermont-based clean energy sector start-up spun off from a U.S. Department of Energy project in 2016 by three University of Vermont electrical engineering faculty, Paul Hines, Mads Almassalkhi and Jeff Frolik.

Global energy powerhouse Siemens is to undergo a fundamental transformation by spinning off its gas and power divisions into a new entity which will also comprise its stake in windpower firm Siemens Gamesa.

Global energy powerhouse Siemens is to undergo a fundamental transformation by spinning off its gas and power divisions into a new entity which will also comprise its stake in windpower firm Siemens Gamesa.

‘Can-kicking’ is a typical, understandable human tendency, but in the employment context it is rarely the right thing to do. Avoiding dealing with behavioural or cultural issues often leads to larger problems, sometimes years down the road. HR profe...

Please find details below of our next HR Summer School which may be of interest to yourself or colleagues. Click here The Summer School is being held at Henley Business School on 7th/8th July 2020 which includes conference dinner and your accom...

The Thai Airways International union has made clear its stance against privatisation and spinning off potentially profitable units as part of a rescue plan for the ailing national carrier.

A well-preserved fossilised tail from Spinosaurus suggests this massive dinosaur may have been able to propel itself and hunt for prey in the water

Robots made of 3D-printed muscle and rat spines could help us understand conditions like motor neurone disease and the technique may eventually be used to build prosthetic devices

A well-preserved fossilised tail from Spinosaurus suggests this massive dinosaur may have been able to propel itself and hunt for prey in the water

Title: Quick Decompression Aids Spinal Injury Recovery
Category: Health News
Created: 4/29/2008 2:00:00 AM
Last Editorial Review: 4/29/2008 12:00:00 AM

Title: Health Tip: Popeye Wasn't Wrong About Spinach
Category: Health News
Created: 5/1/2013 8:35:00 AM
Last Editorial Review: 5/1/2013 12:00:00 AM

Title: Lumbar Puncture (LP or Spinal Tap)
Category: Procedures and Tests
Created: 10/13/1998 12:00:00 AM
Last Editorial Review: 12/13/2019 12:00:00 AM

Title: On Some Farms, Washing Machines Give Leafy Greens a Spin -- But Is That Safe?
Category: Health News
Created: 4/24/2020 12:00:00 AM
Last Editorial Review: 4/24/2020 12:00:00 AM

Objective

To investigate the pathogenicity of the TGM6 variant for spinocerebellar ataxia 35 (SCA35), which was previously reported to be caused by pathogenic mutations in the gene TGM6.