Izegem zet verder koers richting coalitie met Vlaams Belang: “We hebben hier een omgekeerd cordon sanitaire”  Gazet van AntwerpenHele verhaal bekijken via Google Nieuws

1. RSF verurteilt israelische Nachrichtenblockade (reporter-ohne-grenzen.de) Die Organisation Reporter ohne Grenzen (RSF) verurteilt den Umgang der israelischen Armee mit Medienschaffenden. “Aus dem Norden des Gazastreifens dringen immer weniger Informationen heraus, und gerade deshalb wird Journalismus immer wichtiger”, so RSF-Geschäftsführerin Anja Osterhaus: “Die israelischen Streitkräfte verhindern zunehmend Bilder und Stimmen von der Realität des Krieges und […]

About the Event Our resident ensemble of Princeton University performance faculty and talented students presents a Sunday-afternoon program of songs with and without words written by female composers on both sides of the Atlantic. Songs for mezzo-soprano and piano by prolific lieder composer Josephine Lang and for mezzo-soprano and mixed chamber ensemble by Dame Ethel Smyth bookend the program, with works for string quartet, solo piano, and flute, viola, and harp, that reference American, Jamaican, and European song and poetry in between. This event is presented by Princeton University Concerts. For a full event listing and tickets, please visit this link.

Photographer Zion Ozeri, whose parents immigrated to Israel from Yemen, documents the last generation of a bygone era in The Jews of Yemen – The Last Generation.

Financial planner Anthony Carlton says his richest clients are teachers and other unassuming professionals who have good money habits.

If you are the type of person that likes to spoil a game from the off then Brighter Shores is… Continue reading Brighter Shores infinite money glitch – get cash rich with all the money you will ever need

The post Brighter Shores infinite money glitch – get cash rich with all the money you will ever need appeared first on ReadWrite.

ByteDance founder Zhang Yiming is China's richest person, with personal wealth of $49.3 billion (R872 billion), an annual rich list showed on Tuesday, although counterparts in real estate and renewables have fared less well.

Martin R. Larsen
Jul 1, 2005; 4:873-886
Technology

Kazuhiro Ishige, Paolo Salani and Asuka Takatsu
Trans. Amer. Math. Soc. 377 (), 5705-5748.
Abstract, references and article information

Jian Li
Proc. Amer. Math. Soc. 152 (), 5207-5217.
Abstract, references and article information

Priyanka Tripathi
Dec 30, 2020; 0:jlr.RA120001190v1-jlr.RA120001190
Research Articles

Microtubules are polymers composed of αβ-tubulin subunits that provide structure to cells and play a crucial role in in the development and function of neuronal processes and cilia, microtubule-driven extensions of the plasma membrane that have sensory (primary cilia) or motor (motile cilia) functions. To stabilize microtubules in neuronal processes and cilia, α tubulin is modified by the posttranslational addition of an acetyl group, or acetylation. We discovered that acetylated tubulin in microtubules interacts with the membrane sphingolipid, ceramide. However, the molecular mechanism and function of this interaction are not understood. Here, we show that in human iPS cell-derived neurons, ceramide stabilizes microtubules, which indicates a similar function in cilia. Using proximity ligation assays, we detected complex formation of ceramide with acetylated tubulin in C. reinhardtii flagella and cilia of human embryonic kidney (HEK293T) cells, primary cultured mouse astrocytes, and ependymal cells. Using incorporation of palmitic azide and click chemistry-mediated addition of fluorophores, we show that a portion of acetylated tubulin is S-palmitoylated. S-palmitoylated acetylated tubulin is colocalized with ceramide-rich platforms (CRPs) in the ciliary membrane, and it is coimmunoprecipitated with Arl13b, a GTPase that mediates transport of proteins into cilia. Inhibition of S-palmitoylation with 2-bromo palmitic acid or inhibition of ceramide biosynthesis with fumonisin B1 reduces formation of the Arl13b-acetylated tubulin complex and its transport into cilia, concurrent with impairment of ciliogenesis. Together, these data show, for the first time, that CRPs mediate membrane anchoring and interaction of S-palmitoylated proteins that are critical for cilium formation, stabilization, and function. 

TG-rich lipoprotein (TRL)-related biomarkers, including TRL-cholesterol (TRL-C), remnant-like lipoprotein particle-cholesterol (RLP-C), and apoC-III have been associated with atherosclerosis. However, their prognostic values have not been fully determined, especially in patients with previous CAD. This study aimed to examine the associations of TRL-C, RLP-C, and apoC-III with incident cardiovascular events (CVEs) in the setting of secondary prevention of CAD. Plasma TRL-C, RLP-C, and total apoC-III were directly measured. A total of 4,355 participants with angiographically confirmed CAD were followed up for the occurrence of CVEs. During a median follow-up period of 5.1 years (interquartile range: 3.9–6.4 years), 543 (12.5%) events occurred. Patients with incident CVEs had significantly higher levels of TRL-C, RLP-C, and apoC-III than those without events. Multivariable Cox analysis indicated that a log unit increase in TRL-C, RLP-C, and apoC-III increased the risk of CVEs by 49% (95% CI: 1.16–1.93), 21% (95% CI: 1.09–1.35), and 40% (95% CI: 1.11–1.77), respectively. High TRL-C, RLP-C, and apoC-III were also independent predictors of CVEs in individuals with LDL-C levels ≤1.8 mmol/l (n = 1,068). The addition of RLP-C level to a prediction model resulted in a significant increase in discrimination, and all three TRL biomarkers improved risk reclassification. Thus, TRL-C, RLP-C, and apoC-III levels were independently associated with incident CVEs in Chinese CAD patients undergoing statin therapy.

For three decades, the LPL–specific monoclonal antibody 5D2 has been used to investigate LPL structure/function and intravascular lipolysis. 5D2 has been used to measure LPL levels, block the triglyceride hydrolase activity of LPL, and prevent the propensity of concentrated LPL preparations to form homodimers. Two early studies on the location of the 5D2 epitope reached conflicting conclusions, but the more convincing report suggested that 5D2 binds to a tryptophan (Trp)-rich loop in the carboxyl terminus of LPL. The same loop had been implicated in lipoprotein binding. Using surface plasmon resonance, we showed that 5D2 binds with high affinity to a synthetic LPL peptide containing the Trp-rich loop of human (but not mouse) LPL. We also showed, by both fluorescence and UV resonance Raman spectroscopy, that the Trp-rich loop binds lipids. Finally, we used X-ray crystallography to solve the structure of the Trp-rich peptide bound to a 5D2 Fab fragment. The Trp-rich peptide contains a short α-helix, with two Trps projecting into the antigen recognition site. A proline substitution in the α-helix, found in mouse LPL, is expected to interfere with several hydrogen bonds, explaining why 5D2 cannot bind to mouse LPL.

The dominant role of CaV2 voltage-gated calcium channels for driving neurotransmitter release is broadly conserved. Given the overlapping functional properties of CaV2 and CaV1 channels, and less so CaV3 channels, it is unclear why there have not been major shifts toward dependence on other CaV channels for synaptic transmission. Here, we provide a structural and functional profile of the CaV2 channel cloned from the early-diverging animal Trichoplax adhaerens, which lacks a nervous system but possesses single gene homologues for CaV1–CaV3 channels. Remarkably, the highly divergent channel possesses similar features as human CaV2.1 and other CaV2 channels, including high voltage–activated currents that are larger in external Ba2+ than in Ca2+; voltage-dependent kinetics of activation, inactivation, and deactivation; and bimodal recovery from inactivation. Altogether, the functional profile of Trichoplax CaV2 suggests that the core features of presynaptic CaV2 channels were established early during animal evolution, after CaV1 and CaV2 channels emerged via proposed gene duplication from an ancestral CaV1/2 type channel. The Trichoplax channel was relatively insensitive to mammalian CaV2 channel blockers ω-agatoxin-IVA and ω-conotoxin-GVIA and to metal cation blockers Cd2+ and Ni2+. Also absent was the capacity for voltage-dependent G-protein inhibition by co-expressed Trichoplax Gβγ subunits, which nevertheless inhibited the human CaV2.1 channel, suggesting that this modulatory capacity evolved via changes in channel sequence/structure, and not G proteins. Last, the Trichoplax channel was immunolocalized in cells that express an endomorphin-like peptide implicated in cell signaling and locomotive behavior and other likely secretory cells, suggesting contributions to regulated exocytosis.

Small admixtures in water, e.g. of metal ions, often act as cell growth regulators. Here we report that enrichment of deuterium content in water, normally found at 8 mm concentration, two-three folds increases cell proliferation and lowers the oxidative stress level as well. Acting as an anti-oxidant, deuterium-enriched water prevents the toxic effect of such oxidative agents as hydrogen peroxide and auranofin. This action is opposite to that of deuterium depletion that is known to suppress cell growth and induce oxidative stress in mitochondria. We thus hypothesize that deuterium may be a natural cell growth regulator that controls mitochondrial oxidation-reduction balance. Because growth acceleration is reduced approximately by half by addition to water a minute amount (0.15%) of ¹⁸O isotope, at least part of the deuterium effect on cell growth can be explained by the isotopic resonance phenomenon. A slight (2-fold) enrichment of deuterium in water accelerates human cell growth. Quantitative MS based proteomics determined changes in protein abundances and redox states and found that deuterium-enriched water acts mainly through decreasing ROS production in mitochondria. This action is opposite to that of deuterium depletion that suppresses cell growth by inducing oxidative stress. Thus deuterium may be a natural cell growth regulator that controls mitochondrial oxidation-reduction balance. The role of isotopic resonance in this effect was validated by further experiments on bacteria.

O-GlcNAcylation, the addition of a single N-acetylglucosamine residue to serine and threonine residues of cytoplasmic, nuclear, or mitochondrial proteins, is a widespread regulatory post-translational modification. It is involved in response to nutritional status and stress and its dysregulation is associated with diseases ranging from Alzheimer’s to diabetes.  While the modification was first detected over thirty-five years ago, research into the function of O-GlcNAcylation has accelerated dramatically in the last ten years due to the development of new enrichment and mass spectrometry techniques that facilitate its analysis.  This article summarizes methods for O-GlcNAc enrichment, key mass spectrometry instrumentation advancements, particularly those that allow modification site localization, and software tools that allow analysis of data from O-GlcNAc modified peptides.

Glycosylation is a prevalent, yet heterogeneous modification with a broad range of implications in molecular biology. This heterogeneity precludes enrichment strategies that can be universally beneficial for all glycan classes. Thus, choice of enrichment strategy has profound implications on experimental outcomes. Here we review common enrichment strategies used in modern mass spectrometry (MS)-based glycoproteomic experiments, including lectins and other affinity chromatographies, hydrophilic interaction chromatography (HILIC) and its derivatives, porous graphitic carbon (PGC), reversible and irreversible chemical coupling strategies, and chemical biology tools that often leverage bioorthogonal handles. Interest in glycoproteomics continues to surge as MS instrumentation and software improve, so this review aims to help equip researchers with necessary information to choose appropriate enrichment strategies that best complement these efforts.

CD4+ T cell responses are crucial for inducing and maintaining effective anti-cancer immunity, and the identification of human leukocyte antigen class II (HLA-II) cancer-specific epitopes is key to the development of potent cancer immunotherapies. In many tumor types, and especially in glioblastoma (GBM), HLA-II complexes are hardly ever naturally expressed. Hence, little is known about immunogenic HLA-II epitopes in GBM. With stable expression of the class II major histocompatibility complex transactivator (CIITA) coupled to a detailed and sensitive mass spectrometry based immunopeptidomics analysis, we here uncovered a remarkable breadth of the HLA-ligandome in HROG02, HROG17 and RA GBM cell lines. The effect of CIITA expression on the induction of the HLA-II presentation machinery was striking in each of the three cell lines, and it was significantly higher compared to interferon gamma (IFN) treatment. In total, we identified 16,123 unique HLA-I peptides and 32,690 unique HLA-II peptides. In order to genuinely define the identified peptides as true HLA ligands, we carefully characterized their association with the different HLA allotypes. In addition, we identified 138 and 279 HLA-I and HLA-II ligands, respectively, most of which are novel in GBM, derived from known GBM-associated tumor-antigens that have been used as source proteins for a variety of GBM vaccines. Our data further indicate that CIITA-expressing GBM cells acquired an antigen presenting cell-like phenotype as we found that they directly present external proteins as HLA-II ligands. Not only that CIITA-expressing GBM cells are attractive models for antigen discovery endeavors, but also such engineered cells have great therapeutic potential through massive presentation of a diverse antigenic repertoire.

The Indiana man accused of killing two teenage girls in a wooded area near Delphi was found guilty on Monday and faces a mid-December sentencing date.

Martijn P. van den Heuvel
Nov 2, 2011; 31:15775-15786
BehavioralSystemsCognitive

The Director of the Smithsonian's Hirshhorn Museum predicts what the museum's collections will hold in the next 40 years Read more at http://www.smithsonianmag.com/specialsections/40th-anniversary/Arts-Bold-New-Direction.html

Richard Saul Wurman, the founder of the popular speaker series, shares his ideas for how to make learning more interesting

Smithsonian magazine commissioned Drew Gardner for a project that connects Black Americans today to their lost ancestry. Read about Gardner’s project and process, as well as more details about the subjects of this incredible series here: https://www.smithsonianmag.com/history/descendants-black-civil-war-heroes-wear-heritage-pride-180983397/ Video produced by Sierra Theobald. Special thanks to Drew Gardner Additional credits: Emma MacBeath, WikiTree US Black Heritage project; Ottawa Goodman, research and coordinator; Sam Dole, Penumbra Foundation; Elizabeth Zuck, set design; Calvin Osbourne, props and costume; Angela Huff, hair and make up; Diego Huerta, Lexia Krebs, behind-the-scenes filming; background prints by Fujifilm USA

National Treasury has said it is considering a wealth tax on high-net-worth individuals to boost government revenue.

Expert says labels, books, and writing centers all help with skill development

In an extraordinary twist that has upended an already unpredictable election, the world's richest man has become Donald Trump's most influential supporter in his bid to return to the White House.

Sandton was a place to be before the pandemic. But footfall in Africa's richest square mile refuses to go back to pre-pandemic levels. Restaurants and hotels around the area are struggling.

Students across Delaware benefitted from multiple enrichment programs this summer designed to enhance literacy skills and overall well-being. Backed by the Delaware Department of Education (DDOE), Governor John Carney, and key community partners, the programs were offered in school districts and charter schools statewide.

Over the last 20+ years, global society has adopted digital devices at scale, and consumer interaction behaviors continually evolve and mature. As analysts, we're uniquely positioned to notice worldwide trends, country-specific nuances and localized market behaviors that can have significant impact on our brand's business goals. This global scope is [...]

SAS Customer Intelligence 360: Enriching analysis with device usage data was published on Customer Intelligence Blog.

A guidance has been issued to the wealthier students with a list of actions they need to follow to create an inclusive environment.

The UN Sustainable Development Goals aim to end global poverty, but poorer countries are struggling to hit them. More help from richer countries is crucial, writes Mazdak Rafaty.

Check here each week to keep up with the latest from John MacArthur's pulpit at Grace Community Church.

Check here each week to keep up with the latest from John MacArthur's pulpit at Grace Community Church.

United Nations — The U.N. Secretary-General appealed Monday to developed nations to make good on their promise of $100 billion a year to support climate action in developing countries, ahead of a November climate review conference in Egypt.   “Funding for adaptation and resilience must represent at least half of all climate finance,” Antonio Guterres told reporters.    Ministers, climate experts and civil society representatives are meeting this week in the Congolese capital, Kinshasa, to prepare the agenda for the November meeting, known as COP27, which will take place in the Red Sea resort of Sharm el-Sheikh from November 6 to 18.    The United Nations says G-20 countries account for 80% of global emissions, but they have been slow to deliver on their $100 billion annual pledge.   “Taken together, current pledges and policies are shutting the door on our chance to limit global temperature rise to 2 degrees Celsius, let alone meet the 1.5-degree goal,” he said of the benchmarks set in the 2015 Paris Climate Agreement.    The U.N. warns that failure to reach those goals would spell climate catastrophe.   “The world can’t wait,” he added. “Emissions are at an all-time high and rising.”   Guterres said every government, business, investor and institution must step up with concrete climate action plans.    “I am urging leaders at the highest level to take full part in COP27 and tell the world what climate action they will take nationally and globally,” the U.N. chief said.    U.S. Special Presidential Envoy for Climate John Kerry is among the leaders in Kinshasa this week.   

Gowan discusses the obstacles to a peace process, including the fact that extremist rebel factions are unlikely to recognize any ceasefire arrived at in Geneva.

to wow with powerpoint richard harrington

From colonialism to AI, this Booker-longlisted novel urges us to wake up to how we treat wild creatures and places