Three-dimensional cryo electron microscopy reconstructions are obtained by extracting information from a large number of projections of the object. These projections correspond to different `views' or `orientations', i.e. directions in which these projections show the reconstructed object. Uneven distribution of these views and the presence of dominating preferred orientations may distort the reconstructed spatial images. This work describes the program VUE (views on uniform grids for cryo electron microscopy), designed to study such distributions. Its algorithms, based on uniform virtual grids on a sphere, allow an easy calculation and accurate quantitative analysis of the frequency distribution of the views. The key computational element is the Lambert azimuthal equal-area projection of a spherical uniform grid onto a disc. This projection keeps the surface area constant and represents the frequency distribution with no visual bias. Since it has multiple tunable parameters, the program is easily adaptable to individual needs, and to the features of a particular project or of the figure to be produced. It can help identify problems related to an uneven distribution of views. Optionally, it can modify the list of projections, distributing the views more uniformly. The program can also be used as a teaching tool.

The pair angle distribution function (PADF) is a three- and four-atom correlation function that characterizes the local angular structure of disordered materials, particles or nanocrystalline materials. The PADF can be measured using X-ray or electron fluctuation diffraction data, which can be collected by scanning or flowing a structurally disordered sample through a focused beam. It is a natural generalization of established pair distribution methods, which do not provide angular information. The software package pypadf provides tools to calculate the PADF from fluctuation diffraction data. The package includes tools for calculating the intensity correlation function, which is a necessary step in the PADF calculation and also the basis for other fluctuation scattering analysis techniques.

Structural modelling of operando pair distribution function (PDF) data of complex functional materials can be highly challenging. To aid the understanding of complex operando PDF data, this article demonstrates a toolbox for PDF analysis. The tools include denoising using principal component analysis together with the structureMining, similarityMapping and nmfMapping apps available through the online service `PDF in the cloud' (PDFitc, https://pdfitc.org/). The toolbox is used for both ex situ and operando PDF data for 3 nm TiO2-bronze nanocrystals, which function as the active electrode material in a Li-ion battery. The tools enable structural modelling of the ex situ and operando PDF data, revealing two pristine TiO2 phases (bronze and anatase) and two lithiated LixTiO2 phases (lithiated versions of bronze and anatase), and the phase evolution during galvanostatic cycling is characterized.

This article demonstrates the feasibility of obtaining accurate pair distribution functions of thin amorphous films down to 80 nm, using modern laboratory-based X-ray sources. The pair distribution functions are obtained using a single diffraction scan without the requirement of additional scans of the substrate or of the air. By using a crystalline substrate combined with an oblique scattering geometry, most of the Bragg scattering of the substrate is avoided, rendering the substrate Compton scattering the primary contribution. By utilizing a discriminating energy filter, available in the latest generation of modern detectors, it is demonstrated that the Compton intensity can further be reduced to negligible levels at higher wavevector values. Scattering from the sample holder and the air is minimized by the systematic selection of pixels in the detector image based on the projected detection footprint of the sample and the use of a 3D-printed sample holder. Finally, X-ray optical effects in the absorption factors and the ratios between the Compton intensity of the substrate and film are taken into account by using a theoretical tool that simulates the electric field inside the film and the substrate, which aids in planning both the sample design and the measurement protocol.

X-ray scattering has become a major tool in the structural characterization of nanoscale materials. Thanks to the widely available experimental and computational atomic models, coordinate-based X-ray scattering simulation has played a crucial role in data interpretation in the past two decades. However, simulation of real-space pair distance distribution functions (PDDFs) from small- and wide-angle X-ray scattering, SAXS/WAXS, has been relatively less exploited. This study presents a comparison of PDDF simulation methods, which are applied to molecular structures that range in size from β-cyclo­dextrin [1 kDa molecular weight (MW), 66 non-hydrogen atoms] to the satellite tobacco mosaic virus capsid (1.1 MDa MW, 81 960 non-hydrogen atoms). The results demonstrate the power of interpretation of experimental SAXS/WAXS from the real-space view, particularly by providing a more intuitive method for understanding of partial structure contributions. Furthermore, the computational efficiency of PDDF simulation algorithms makes them attractive as approaches for the analysis of large nanoscale materials and biological assemblies. The simulation methods demonstrated in this article have been implemented in stand-alone software, SolX 3.0, which is available to download from https://12idb.xray.aps.anl.gov/solx.html.

A procedure is presented to exactly obtain the apparent average crystallite size (ACS) of powder samples using standard in-house powder diffraction experiments without any restriction originating from the Scherrer equation. Additionally, the crystallite size distribution within the sample can be evaluated. To achieve this, powder diffractograms are background corrected and long-range radial distribution functions G(r) up to 300 nm are calculated from the diffraction data. The envelope function fenv of G(r) is approximated by a procedure determining the absolute maxima of G(r) in a certain interval (r range). Fitting of an ACS distribution envelope function to this approximation gives the ACS and its distribution. The method is tested on diffractograms of LaB6 standard reference materials measured with different wavelengths to demonstrate the validity of the approach and to clarify the influence of the wavelength used. The latter results in a general description of the maximum observable average crystallite size, which depends on the instrument and wavelength used. The crystallite site distribution is compared with particle size distributions based on transmission electron microscopy investigations, providing an approximation of the average number of crystallites per particle.

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Scientists have recovered the first genetic data from an extinct bird in the Caribbean, thanks to the remarkably preserved bones of a Creighton's caracara in a flooded sinkhole on Great Abaco Island in the Bahamas. Studies of ancient DNA from tropical birds have faced two formidable obstacles. Organic material quickly degrades when exposed to heat, light and oxygen. And birds' lightweight, hollow bones break easily, accelerating the decay of the DNA within. But the dark, oxygen-free depths of a 100-foot blue hole known as Sawmill Sink provided ideal preservation conditions for the bones of Caracara creightoni, a species of large carrion-eating falcon that disappeared soon after humans arrived in the Bahamas about 1,000 years ago. Florida Museum of Natural History researcher Jessica Oswald and her colleagues extracted and sequenced genetic material from the 2,500-year-old C. creightoni femur. Because ancient DNA is often fragmented or missing, the team had modest expectations for what they would find –- maybe one or two genes. But instead, the bone yielded 98.7% of the bird's mitochondrial genome, the DNA most living things inherit from their mothers. The mitochondrial genome showed that C. creightoni is closely related to the two remaining caracara species alive today: the crested caracara and the southern caracara. The three species last shared a common ancestor between 1.2 and 0.4 million years ago. "This project enhanced our understanding of the ecological and evolutionary implications of extinction, forged strong international partnerships, and trained the next generation of researchers," says Jessica Robin, a program director in National Science Foundation's Office of International Science and Engineering, which funded the study.

Image credit: Florida Museum photo by Kristen Grace

NASA released a workshop report on the UNBOUND-FEW workshop series, which was facilitated in part by Tribal Resilience Learning Network staff from the Alaska CASC. The workshop report reveals key recommendations for making data tools more useful for climate adaptation planning.

Photo of Marcell Jankovics; Credit: courtesy of cartoonbrew.com

FilmWeek’s animation authority, Charles Solomon, remembers the great Hungarian animator Marcell Jankovics, who died on May 29. He died at the age of 79. Our critics also share some of the things they’ve been busy watching on various streaming platforms and why they recommend listeners check them out.  

Guests:

Amy Nicholson, film critic for KPCC, film writer for The New York Times and host of the podcast ‘Unspooled’ and the podcast miniseries “Zoom”; she tweets @TheAmyNicholson

Wade Major, film critic for KPCC and CineGods.com

Charles Solomon, film critic for KPCC, Animation Scoop and Animation Magazine

This content is from Southern California Public Radio. View the original story at SCPR.org.

RealtyAnalytix Advisors, LLC announces the introduction of Catawba Industrial Commons, a multi-tenant industrial campus offering the most attractive, affordable and functional manufacturing, distribution and warehousing space in the Greater Catawba County region.

Catawba County Assistant Planning Director, Mary George, has been named 2012 Outstanding Contributor to Agriculture by the Hickory Kiwanis Club

Smokers who are exposed to radon appear to be at even greater risk for lung cancer, because the effects of smoking and radon are more powerful when the two factors are combined, says a new report by a committee of the National Research Council.

Bacteria that resist antibiotics can be passed from food animals to humans, but not enough is known to determine the public health risks posed by such transmission, says a new report by a committee of the National Research Council.

It is now possible to estimate the influence of climate change on some types of extreme events, such as heat waves, drought, and heavy precipitation, says a new report from the National Academies of Sciences, Engineering, and Medicine.

Current federal safety regulations for small distribution systems used for propane and other liquefied petroleum gases (LPGs) should be improved for clarity, efficiency, enforceability, and applicability to risk, says a new report from the National Academies of Sciences, Engineering, and Medicine.

For severe acute pain due to surgeries and medical conditions, there is a lack of guidance on the appropriate type, strength, and amount of opioid medication that clinicians should prescribe to patients, says a new report from the National Academies of Sciences, Engineering, and Medicine.

The use of compounded bioidentical hormone therapies (cBHTs) — an increasingly popular approach to relieve symptoms of menopause and male hypogonadism — should be limited to the small number of patients who cannot benefit from an FDA-approved hormone therapy product, says a new report from the National Academies of Sciences, Engineering, and Medicine.

The National Academy of Medicine today announced Stephen P. Hinshaw is the recipient of the 2020 Rhoda and Bernard Sarnat International Prize in Mental Health, for basic and applied research on individuals with externalizing disorders, and for efforts to reduce mental illness stigma through youth-based programs and the promotion of humanization.

The National Academies of Sciences, Engineering, and Medicine today released the final report of a consensus study recommending a four-phased equitable allocation framework that the U.S. Department of Health and Human Services (HHS) and state, tribal, local, and territorial (STLT) authorities should adopt in the development of national and local guidelines for COVID-19 vaccine allocation.

The United States should create a national strategy by end of 2022 to reduce its outsized and increasing contribution to plastic waste in the world’s oceans.

The Action Collaborative on Preventing Sexual Harassment in Higher Education today released three new individually authored publications to serve as resources for higher education institutions as they work to prevent sexual harassment and mitigate the damage it causes.

The Gulf Research Program of the National Academies of Sciences, Engineering, and Medicine was an active contributor at the first in-person Gulf of Mexico Conference (GoMCon) held in Baton Rouge, Louisiana, from April 25-28. The four-day conference gathered more than 800 researchers, resource managers, and interested stakeholders, and highlighted the intersection of scientific research and the management of human and natural systems in the Gulf of Mexico region.

Congress should take action to facilitate the participation of all land-grant colleges and universities in multistate research projects, says a new report. Funding disparities have prevented many historically Black and tribal colleges and universities from fully collaborating with other land-grant institutions.

Two remarkable women who made invaluable contributions to the work of the Committee on International Security and Arms Control (CISAC) died in recent months -- Catherine Kelleher and Jo Husbands. Their accomplishments and qualities have been described well by others, including especially Women in International Security (WIIS). We at CISAC want additionally to honor their work for us.

Attributing a cyberattack to a specific threat actor is a complex affair, as evidenced by new ESET research published this week

By adjusting global cybersecurity tools and strategies to fit Indian requirements, distributors help companies of all sizes -- small businesses to large corporations -- adopt security solutions that are practical, effective, and scaled appropriately for their environments.

The owners of the company operated Nature’s Bakery before selling to Kind Snacks.

Direct store delivery, third-party logistics often popular with snack manufacturers.

RIBUS Inc closed out 2022 with the celebration of 30 years of business serving the food, beverage, pet, and dietary supplement sectors with clean-label ingredients to companies around the world.

Third-party logistics (3PL) can be useful when snack and bakery companies need to outsource logistics and distribution needs.

As history has proven in the baking industry, sometimes distribution can make or break a company. Through the years, direct-store delivery (DSD) routes have grown increasingly complex, as the market for drivers has become exponentially more competitive. Meanwhile, complete transparency and tracking of actionable data for analyzing productivity are increasingly standard industry features.

The gourmet brand will now be sold in 90 locations of the North Carolina chain.

Acquisition enhances Batory Foods’ logistical efficiency with the addition of strategically located cold storage warehouse.

Trio of chocolate treats now available at Harris Teeter, Giant Eagle, QuickChek, and Amazon.

FreshFry has announced its Canadian expansion, bringing its efficient and eco-friendly FreshFry Pods to commercial kitchens across Canada.

The initial offering will include 6-oz. resealable bags for retail as well as 2-lbs. bags perfect for foodservice applications.

Beginning in April, the company's eco-friendly tortilla chips will hit shelves in Sprouts' Innovation Centers.

Exclusive partnership will scale next generation chickpea proteins for North America.

No longer relegated to taco night, tortillas are playing a major role in Americans' meal plans, both at home and when dining out.

The Minnetonka, Minn.-based food solutions and potassium chloride supplier strikes a deal with Barentz Europe B.V., which is present in 26 countries in Europe, to distribute its Advanced Formula potassium chloride to food manufacturers in Europe. Nu-Tek says that governments in the U.K. and the Netherlands are close to making mandatory rules regarding sodium levels in food products with the aim to reduce high blood pressure and cardiovascular disease.

Nu-Tek Food Science, Minnetonka, Minn., has signed an agreement with Tovani Benzaquen Ingredients, Sao Paulo, to distribute Nu-Tek Salt Advanced Formula Potassium Chloride to food manufacturers in Brazil.

A new report from the Centers for Disease Control and Prevention (CDC) reveals that nearly half of children’s sodium intake comes from 10 foods, including bread and rolls.

Better-for-you tortillas available at select Sprouts, Shop Rite, and Wegmans locations.

The BFY frozen food producer is bringing new snacks and veggie burgers to Targets.

Bimbo Bakehouse recently introduced Entenmann’s Little Bites for foodservice distribution in both Chocolate Chip and Blueberry varieties.

This week's Fun Friday includes a Nutella and Betches book club partnership, Nerds at the Big Game, Pillsbury partnering on candles, a Cheez-It college tailgating food truck, and Haribo's float debut at Macy's Thanksgiving Day parade.

Expanding its global distribution presence, Prinova Group LLC has entered into a definitive agreement to acquire industry expert The Ingredient House, LLC.

The snack and bakery sweetener will be added to Batory’s line of ingredient offerings.

The agreement will expand the availability of Incredo Sugar to U.S. food companies.

Artisanal buckwheat snack producer Better With Buckwheat is expanding into six regional and ecommerce grocery chains through June and July.