Stanley Lake with McGown Peak in the background.

Gelada Monkey staring into the sunset

Yamagata February, the winter was approaching the end in the flatland. I was aiming for the summit of "Mt.zao" from the night. There is a beautiful crater lake which is still active as a volcano, called "Goshikinuma", and in the summer it is a beautiful lake of emerald green. It was covered with strong wind and fog, but it showed its appearance in a momentary fine weather. The crater lake where the accumulated snow began to melt was like a blue moon.

Morning Routines at the bund. At the bund in shanghai every morning a group of old people practice Tai Chi, an ancient marital arts form in china.

It was a quiet morning at Nagarahole Tiger Reserve, Sun was opening up and the mist from the night was clearing. As we made a drive through the wood we saw this beautiful leopardess perching on her favourite tree. This leopardess was marking her presence by rubbing her scent glands to the tree trunk by hugging it. This behaviour was very interesting to witness as it was marking her scent on the top branches of the tree. This is one of the favourite pictures that I took recently because of the whole setup. Artistically one can visualize the tree branch as an "Arm of the Mother Nature" and leopard is holding on to it. It shows the unmitigated bonding shared between the tree and the Leopard.

The proboscis monkey is a species endemic to Borneo. I areas where they occur they are quite easy to encounter as they have reddish fur and make strange burping sounds for communication.

Monuments in winter

When the full moon rises, dark skies shine with bright fire, and the stars go to the dance, as if greeting it. Kazakhstan

Magenta skies over Monument Valley at sunset.

The third installment in our weekly series spotlights titles that may have been lost in the news amid the COVID-19 crisis

Seventy-five years after a memorable mission, the B26 bomber 'Flak-Bait' undergoes preservation at the National Air and Space Museum

In the early 20th century, Native people responded to the proclamation of Mother’s Day with powwows, ceremonies, rodeos, feasts, and songs

The remote cabin could be the difference between life and death for travellers stuck on the territory’s long winter road. It was built to remember Stella Barnaby, who would have been 55 this Saturday.

If New Brunswickers were planning on doing any yard work this weekend they will likely be using their snow shovels instead of their lawn rakes. 

It looks like New Brunswickers will see more snow Saturday with a snowfall warning issued for central and northern parts of the province. 

Some farmers across the province are worried about getting their fuel in time for spring seeding. The Agricultural Producers Association of Saskatchewan says it has been fielding complaints this week about delays at the Co-op cardlock near Moose Jaw.

A large destination resort in Minnesota, just a few hours south of Thunder Bay, Ont., has some big expansion plans.

The Computer Emergency Response Team of Mauritius (CERT-MU) in collaboration with the Command and Control Centre of Kenya organised a 3-day training programme on Cyber Defense Monitoring and Forensics at Voilà Hotel, Bagatelle from the 27^th February – 1^st March 2018. The training course provided an introduction to Network Security Monitoring (NSM), Security Information and Events Management (SIEM), Malware Analysis and Digital Forensics. Major part of the course was hands-on case studies and analysis exercises using real world data. The main focus of the training programme was on intensive hands-on sessions on addressing key challenges faced by local organizations in all sectors/industries. A wide range of commercial and open source tools were used to equip cyber defenders with the necessary skills to anticipate, detect, respond and contain adversaries. The training programme was followed by 23 participants from the public and private sector. 


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Newfoundland and Labrador's already fragile economy suffered another gut-punch in April, with Statistics Canada reporting Friday that 29,000 jobs were lost in April alone.

Long-term care homes in Eastern Health's jurisdiction are allowing for people to again visit their relatives by windows, just in time for Mother's Day. 

Green Depots will begin to reopen across the province on Monday, according to the Multi-Materials Stewardship Board, though dates and hours of operation may vary between sites. 

The province is still on track to move to a lower alert level Monday, which would ease some of the public health measures in place aimed at curbing COVID-19.

64-year-old John Neville, in agony and needing his wisdom tooth extracted, has been navigating a complex tangle of COVID-19 restrictions in an unsuccessful attempt at getting emergency dental care.

Staff halted $100 service over pandemic concerns, forcing homeowners to private plumbers much more, and Danny Breen is urging them to contact the city.

"If you are starting out on the language and culture learning journey or if you have been at it a while and are feeling tired and despondent, I encourage you to persevere. To stick with it and pray for strength to continue. Keep asking questions and keep learning about the culture," urges Beth.

Mar del Plata, Argentina :: Crewmembers visit a Christian rehabilitation centre and remind women of their value in God's eyes.

Mar del Plata, Argentina :: Crew work alongside a pastor who has plans to improve children's futures.

The vessel, formerly known as the Edmonton Queen, got skewered by its own moorings last month as the icy North Saskatchewan River began to thaw.

Much like a prehistoric pest trapped in amber, our summer plans remain in suspended animation.

In a new twist on oral history, University of Alberta paleontologists have discovered that an Argentinian reptile from 95 million years ago developed a type of tooth enamel that is common in humans and other mammals but rare among reptiles.

Police are investigating a report of a suspicious package in north Edmonton.

Just across town from the Library of Congress in Washington, D.C., stands Gallaudet University, an institute for higher learning for the deaf and hard-of-hearing. In 1864, President Abraham Lincoln signed the law that allowed the school to begin issuing college degrees, a milestone for deaf people seeking higher education. Edward M. Gallaudet (right) was the […]

Successful modification of the bacterial artificial chromosome (BAC) after two-step BAC engineering is confirmed in two separate polymerase chain reactions (PCRs). The first reaction (5' co-integrate PCR) uses a forward 5' co-integrate primer (a sequence located upstream of the 5' end of the A-box) and a reverse 3' primer on the vector (175PA+50AT) or within the reporter sequence or mutated region as appropriate. The second reaction (3' co-integrate PCR) uses a forward 5' primer on the recA gene (RecA1300S) and a reverse 3' co-integrate primer (a sequence located downstream from the 3' end of the B-box). Those colonies shown to be positive in PCR analysis are further tested for sensitivity to UV light. After the resolution, colonies that have lost the excised recombination vector including sacB and recA genes become UV light sensitive.

Bacterial artificial chromosome (BAC) clones are rendered electrocompetent and transformed with the recombinant shuttle vector, pLD53SCAB/AB-box. Cointegrates are selected by growth on chloramphenicol and ampicillin to ensure recombination of the shuttle vector into the BAC.

Plasmid DNA is prepared from the recombinant shuttle vector pLD53.SCAB/A-B created by cloning of the A and B homology arms for two-step bacterial artificial chromosome (BAC) engineering. To confirm that the A-box and B-box arms have been successfully incorporated into pLD53.SCAB, the pattern of enzyme digestion of the modified plasmid is compared with that of the unmodified pLD53.SCAB. Once the shuttle vector is shown to carry the proper sequences, it is ready for transfer into the BAC host.

This protocol describes the preparation of the shuttle vector before its introduction into bacterial artificial chromosome (BAC) host cells for BAC two-step engineering. The homology arm sequences, prepared previously, are introduced by ligation into the digested shuttle vector DNA to provide sites for recombination within the BAC clone. Crude lysates of individual bacterial transformants serve as templates in polymerase chain reaction (PCR) analysis to confirm the presence of the homology arms in the recombinant shuttle vector.

The 700-bp A homology arm (A-box) and the 700-bp B homology arm (B-box) are amplified by polymerase chain reaction (PCR) using purified bacterial artificial chromosome (BAC) DNA as template for two-step BAC engineering. The resulting A-box PCR product contains an AscI site at its 5' end (the 5' primer incorporates an AscI site, and the 3' primer does not incorporate any restriction sites). The B-box PCR product contains an XmaI site at its 3' end (the 5' primer does not incorporate any restriction sites, and the 3' primer incorporates an XmaI site). The amplification products are then digested with the appropriate restriction endonucleases to render them suitable for cloning into the shuttle vector.

In two-step bacterial artificial chromosome (BAC) engineering, a single plasmid is introduced into the BAC-carrying cell lines. The shuttle vector pLD53.SCAB (or pLD53.SCAEB) carries the recA gene and the R6K origin, which requires the protein to replicate. PIR2 cells, expressing , are typically used for the amplification of the vector and maintain about 15 copies/cell of the donor vector, which is relatively stable in this host.

We assess the dynamics of volatility spillovers among global systemically important banks (G-SIBs). We measure spillovers using vector-autoregressive models of range volatility of the equity prices of G-SIBs, together with machine learning methods. We then compare the size of these spillovers with the degree of systemic importance measured by the Basel Committee on Banking Supervision's G-SIB bucket designations.