In insects, oocyte resorption (oosorption) or follicular atresia is one of the key physiological processes and evolutionary strategies used to optimize reproductive fitness. Mosquitoes are ideal model organisms for studying egg maturation in arthropods, as their follicle development is initiated only following the ingestion of a blood meal, followed by a carefully orchestrated series of hormonally regulated events leading to egg maturation. A cohort of approximately 100 follicles per mosquito ovary begin developing synchronously. However, a significant fraction of follicles ultimately undergo apoptosis and oosorption, especially when available resources from the blood meal are limited. Therefore, simple, rapid, and reliable techniques to accurately evaluate follicular atresia are necessary to understand mechanisms underlying follicle development in insects. This protocol describes how to detect apoptotic follicle cells within the Aedes aegypti mosquito ovaries using a commercially available fluorescent-labeled inhibitor of caspases (FLICA). Caspases are key players in animal apoptosis. In this assay, the FLICA reagent enters the intracellular compartment of follicles in dissected mosquito ovaries and covalently binds to active caspases. The bound reagent remains within the cell and its fluorescent signal can be observed by confocal microscopy. Although this method was specifically developed for visualizing apoptotic ovarian follicles during Ae. aegypti mosquito egg development, it should be applicable to other mosquito tissues that undergo caspase-mediated program cell death in a time-dependent manner.

After more than a century of searching, scientists have discovered a gene in snails that may control asymmetries inside many animals

An unlikely friendship with Guy Burgess, the infamous British double-agent, brought unexpected joy to Stanley Weiss

More than 50 Norman Wells residents gathered at the Royal Canadian Legion on Thursday night to voice frustrations over high heating fuel prices.

Former Dawson City Mayor Bill Kendrick has filed his defence against the town's encroachment violation lawsuit.

Wanita Johnson, who celebrates her birthday this weekend in Whitehorse, still has a sharp memory for how the Second World War helped shape her life.

Stephanie Yuill spent five weeks visiting First World War sites across Europe on a bike she bought from the grocery store. 

A man who fled from police, drove onto a walking trail, triggered a shelter-in-place advisory for the Chateau Nova hotel in Yellowknife and subsequently broke into a home was on bail at the time and under house arrest, police say.

In Fort Good Hope, N.W.T., work is underway on a modular home construction centre that local leaders say will help the community meet housing and employment goals.

Historians say that Sarah Emma Edmonds exaggerated many aspects of her wartime experiences. Still, she bravely served in the Union Army, becoming one of hundreds of women who fought in the conflict in secret

Newspaper editor Horace Greeley unsuccessfully ran against incumbent Ulysses S. Grant in November 1872. Twenty-four days later, he died of unknown causes at a private mental health facility

Fifty years ago, on October 5, 1974, David Kunst completed the first verified circumnavigation of the globe on foot. Along the way, he met Princess Grace of Monaco, raised money for UNICEF and lost a brother to bandits

An exhibition at the Huntington Library, Art Museum and Botanical Gardens explores how Western intellectuals viewed the climate crisis between 1780 and 1930

The British explorer named dozens of geographical features and sites in the region, ignoring the traditions of the Indigenous peoples who’d lived there for millennia

When the U.S. Army massacred a Lakota village at Blue Water, dozens of plundered artifacts ended up in the Smithsonian. The unraveling of this long-buried atrocity is forging a path toward reconciliation

With flinty perseverance and a golden touch, Belinda Mulrooney earned an unlikely fortune in the frozen north and reshaped the Canadian frontier

The 1898 Wilmington massacre left dozens of Black North Carolinians dead. Conspirators also forced the city's multiracial government to resign at gunpoint

A jury has found a 20-year-old Moncton man guilty of second-degree murder in the 2022 death of an 18-year-old.

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Interim PC Leader Glen Savoie urged members to find a common purpose rather than blame former leader Blaine Higgs.

Emergency officials said the fire at the Herring Pub and Restaurant on Water Street broke out around 4 p.m. and appears to have started in an air conditioning unit.   

A date has been set for an inquest into the circumstances surrounding the death of a 37-year-old man in police custody in Atikokan, Ont. who overdosed while in police custody in October 2020. The incident led to an investigation by the province's Special Investigations Unit.

As a troubled local gym goes dark, some members and the landlord say they haven’t heard from the owner about the future of the business in Thunder Bay, Ont.

"If you are starting out on the language and culture learning journey or if you have been at it a while and are feeling tired and despondent, I encourage you to persevere. To stick with it and pray for strength to continue. Keep asking questions and keep learning about the culture," urges Beth.

An OM worker from Latin America uses his carpentry skills to disciple young men in their faith and give them better employment opportunities for the future.

How do you encapsulate everything that goes into a full week of teaching, worship music, workshops, meals and so much more? Enjoy a brief overview of TeenStreet 2019.

Serving in West and Central Asia for many years, Paul and Soonok have one lasting dream: to see a church planting movement there.

A man from Calgary has been charged in the death of a worker after a 24-year-old woman sustained fatal injuries when a pickup truck crashed into a pizza restaurant in Fort McMurray. 

A man from Queens County has been sentenced to five years and nine months in federal prison for charges including making and transmitting child pornography, voyeurism and intercepting private communications.

P.E.I.'s capital budget has allocated $2.5 million to build up to five provincially owned cellphone towers, but so far no telecommunications companies have committed to using them.

The Town of Kensington, P.E.I., has committed to implementing a new campaign to end gender-based violence.

With P.E.I's only full-time blood oncologist leaving at the end of November, Health P.E.I. was planning to transfer his patients to Dr. Philip Champion. Now Champion says he intends to retire in the spring.

A 22-year-old man from British Columbia has been charged on P.E.I. after hundreds of grams of fentanyl and cocaine were seized in Charlottetown on Saturday night.

Juanita Migwans of M'Chigeeng First Nation on Manitoulin Island has been missing for over a month, but her aunt says she’s not giving up on the search.

Sault Ste. Marie has the highest rate of opioid deaths in Ontario so far this year, according to new numbers from the province's coroner's office.

A federal audit of Canada’s strategy to promote the extraction of critical minerals says the government isn’t giving due consideration to the potential impact of mining on the environment, biodiversity and Indigenous people.

A retired lawyer from Sault Ste. Marie has written a book that celebrates the working-class neighbourhood in which he grew up. The West End: A Magical Place Created by Giants is by Frank Sarlo, whose grandfather emigrated to Chicago from Italy, but ended up in Sault Ste. Marie with the promise of work on the railroad.

Éric Boutilier and Mark Sherry are launching their new board game, Tripped, which was five years in the making.

A Sudbury pharmacy technician has come up with a better way to do her job, while also improving patient safety. Now her method is being used at Sudbury's hospital, and is getting interest from others.

The large urban mayors of Northern Ontario are advocating for increased provincial and federal support, prioritizing the establishment of Homelessness and Addiction Recovery Treatment Hubs and the expansion of the Rural Community Immigration Pilot.

An 18-year-old man charged with attempted murder in a small northern Ontario community made a brief court appearance today by video, and remains in custody. Meanwhile, community members gathered outside the courthouse for a rally.

The crowning achievement at last year's COP climate conference in Dubai was a global consensus on the need to "transition away from fossil fuels." This year, the conference had not even started yet before BBC News exposed senior organizers of using the event to arrange potential deals for fossil fuel expansion. The recent election of Donald Trump in the U.S. has added an additional layer of uncertainty to this year's meeting.

Medical student Samuel Bonne had read everything he could to prepare for his group's dissection of a cadaver. But after the first cut, he left with lingering questions about what it means to lead a good life and die with dignity.

A missing 26-year-old Sagkeeng First Nation man has been found dead in the community of Fort Alexander, about 100 kilometres northeast of Winnipeg, Powerview RCMP say. Christian Letander's body was found Monday near the Winnipeg River.

Winnipeg police are investigating the death of a 23-year-old man who was fatally injured in Winnipeg on Friday.  

Over the weekend, RCMP says officers searched for answers in the death of the toddler, knocking door-to-door on Saturday in Ashern and the surrounding communities of Gypsumville as they canvassed for information that may shed light on the timeline surrounding the child's death.

RCMP say a 23-year-old woman was found dead in a residence in central Manitoba on Tuesday. 

Team Reid Carruthers has started the search for a new second after Derek Samagalski's decision to step back from the Winnipeg-based rink.

CBC Winnipeg News host Brittany Greenslade speaks with Canadian gynecologist Dr. Jen Gunter, who is part of a Tuesday panel discussion about health misinformation and how to separate fact from fiction. It's part of Together Against Misinformation Week, a nationwide event that runs until Nov. 17.

The Manitoba government made a "political compromise" in its decision to issue a reduced number of moose hunting licences to non-Indigenous hunters in northern parts of the province earlier this year, a lawyer representing a First Nation in the area has argued in court.