“His ultimate passion was for good governance and his advocacy was to help save the environment. He was a highly principled man who sought refuge in his faith.”

The post Dumaguete mourns death of broadcaster 2 days after World Press Freedom Day appeared first on Bulatlat.

“We are alarmed that soon, other media outlets would follow. Without the freedom of the press, there is the danger of other freedoms to be lost. God forbid, these are the first 'test signs' of curtailing other rights.”

The post ABS-CBN shutdown may lead to curtailing of more rights, says press freedom fighter Edita Burgos appeared first on Bulatlat.

“Di ko akalain na ang kalayaan na na-achieve natin noong 1986 ay manganganib uli ngayon.” (I did not expect that the freedom we had achieved in 1986 would be threatened once again.)

The post Veteran journalists urge the public to defend press freedom, democratic rights appeared first on Bulatlat.

LONDON: Planning for future pandemics should be handed over to the NHS and be “free from political interference”, academics have suggested.Researchers from Glasgow Caledonian University, the Cass Business School in London, Nottingham University and Vlerick Business School, in Belgium...

Simmering trade disputes are decreasing the beneficial effects of free trade and could in the long-term damage the rules-based order upon which global commerce is based.

With key reforms, Pacific states could move toward cleaner, more affordable sources of energy that eventually eliminate fossil fuels completely.

PUNE: The Pune Municipal Corporation (PMC) on Saturday demolished illegal constructions spread over 4,000 sq ft in Warje. Civic officials said these included residential and commercial establishments. The premises in the side and front margins were occupied without permission from the civic administration. In February, the civic administration had acted against encroachments in Ambegaon, Bavdhan and Yerawada. Illegal constructions over 1,432 sq ft, 9,000 sq ft and 4,632 sq ft, receptively were demolished in the drive. The civic body has acted against over 3,300 illegal constructions in the last year. As part of the drive, illegal sheds and houses built in concrete on an area of 22 lakh sq ft […]

Einstein’s framework for the universe, space-time, is at odds with quantum theory. Overcoming this clash and others is vital to unravelling the true nature of the cosmos

The bell of an upside-down jellyfish has structures that can provide a scaffold for growing human skin cells, which could be used to help repair wounds after surgery or a bad burn

All you need to make satisfying plant-based protein is flour and water. Seitan, or wheat gluten, takes a little effort to produce, but the results are worth is, says Sam Wong

In Washington State and Boston, two initiatives are helping prevent potatoes from being thrown out and milk from being poured down the drain. Gavino Garay has more.

In Washington State and Boston, two initiatives are helping prevent potatoes from being thrown out and milk from being poured down the drain. Gavino Garay has more.

Title: Smoke-Free Laws May Help Prevent COPD Hospitalizations
Category: Health News
Created: 4/25/2014 2:35:00 PM
Last Editorial Review: 4/28/2014 12:00:00 AM

Title: 'Freezing' Technique May Work for Some Women With Early Breast Cancer
Category: Health News
Created: 4/30/2014 2:36:00 PM
Last Editorial Review: 5/1/2014 12:00:00 AM

Title: Eating Gluten-Free Without a Medical Reason?
Category: Health News
Created: 5/2/2017 12:00:00 AM
Last Editorial Review: 5/3/2017 12:00:00 AM

Title: LA First Major U.S. City to Offer Free Coronavirus Tests
Category: Health News
Created: 4/30/2020 12:00:00 AM
Last Editorial Review: 4/30/2020 12:00:00 AM

Title: An Allergist Offers His Expert Advice for a Sneeze-Free Spring
Category: Health News
Created: 3/7/2020 12:00:00 AM
Last Editorial Review: 3/9/2020 12:00:00 AM

Adipocytes take up long chain FAs through diffusion and protein-mediated transport, whereas FA efflux is considered to occur by diffusion. To identify potential membrane proteins that are involved in regulating FA flux in adipocytes, the expression levels of 55 membrane transporters without known function were screened in subcutaneous adipose samples from obese patients before and after bariatric surgery using branched DNA methodology. Among the 33 solute carrier (SLC) transporter family members screened, the expression of 14 members showed significant changes before and after bariatric surgery. One of them, Slc43a3, increased about 2.5-fold after bariatric surgery. Further investigation demonstrated that Slc43a3 is highly expressed in murine adipose tissue and induced during adipocyte differentiation in primary preadipocytes and in OP9 cells. Knockdown of Slc43a3 with siRNA in differentiated OP9 adipocytes reduced both basal and forskolin-stimulated FA efflux, while also increasing FA uptake and lipid droplet accumulation. In contrast, overexpression of Slc43a3 decreased FA uptake in differentiated OP9 cells and resulted in decreased lipid droplet accumulation. Therefore, Slc43a3 seems to regulate FA flux in adipocytes, functioning as a positive regulator of FA efflux and as a negative regulator of FA uptake.

OBJECTIVE

Debate continues regarding the influence of dietary fats and sugars on the risk of developing metabolic diseases, including insulin resistance and nonalcoholic fatty liver disease (NAFLD). We investigated the effect of two eucaloric diets, one enriched with saturated fat (SFA) and the other enriched with free sugars (SUGAR), on intrahepatic triacylglycerol (IHTAG) content, hepatic de novo lipogenesis (DNL), and whole-body postprandial metabolism in overweight males.

In Lysobacter enzymogenes OH11, RpfB1 and RpfB2 were predicted to encode acyl coenzyme A (CoA) ligases. RpfB1 is located in the Rpf gene cluster. Interestingly, we found an RpfB1 homolog (RpfB2) outside this canonical gene cluster, and nothing is known about its functionality or mechanism. Here, we report that rpfB1 and rpfB2 can functionally replace EcFadD in the Escherichia coli fadD mutant JW1794. RpfB activates long-chain fatty acids (n-C_16:0 and n-C_18:0) for the corresponding fatty acyl-CoA ligase (FCL) activity in vitro, and Glu-361 plays critical roles in the catalytic mechanism of RpfB1 and RpfB2. Deletion of rpfB1 and rpfB2 resulted in significantly increased heat-stable antifungal factor (HSAF) production, and overexpression of rpfB1 or rpfB2 completely suppressed HSAF production. Deletion of rpfB1 and rpfB2 resulted in increased L. enzymogenes diffusible signaling factor 3 (LeDSF3) synthesis in L. enzymogenes. Overall, our results showed that changes in intracellular free fatty acid levels significantly altered HSAF production. Our report shows that intracellular free fatty acids are required for HSAF production and that RpfB affects HSAF production via FCL activity. The global transcriptional regulator Clp directly regulated the expression of rpfB1 and rpfB2. In conclusion, these findings reveal new roles of RpfB in antibiotic biosynthesis in L. enzymogenes.

IMPORTANCE Understanding the biosynthetic and regulatory mechanisms of heat-stable antifungal factor (HSAF) could improve the yield in Lysobacter enzymogenes. Here, we report that RpfB1 and RpfB2 encode acyl coenzyme A (CoA) ligases. Our research shows that RpfB1 and RpfB2 affect free fatty acid metabolism via fatty acyl-CoA ligase (FCL) activity to reduce the substrate for HSAF synthesis and, thereby, block HSAF production in L. enzymogenes. Furthermore, these findings reveal new roles for the fatty acyl-CoA ligases RpfB1 and RpfB2 in antibiotic biosynthesis in L. enzymogenes. Importantly, the novelty of this work is the finding that RpfB2 lies outside the Rpf gene cluster and plays a key role in HSAF production, which has not been reported in other diffusible signaling factor (DSF)/Rpf-producing bacteria.

In winter, the veins of leaves don’t get completely covered in frost. Mimicking this effect could be the best way to create ice-resistant surfaces

BSNL का यह ऑफर सिर्फ 90 दिनों के लिए है और ऑफर की शुरुआत एक अप्रैल से हो चुकी है। BSNL का यह ऑफर सभी सर्किल के लिए उपलब्ध है। आप किसी भी स्टोर पर जाकर अपना पुराना 2जी या 3जी सिम दे सकते हैं और नया 4जी सिम कार्ड ले सकते हैं।

Grand Theft Auto Online is giving away the GTA Alien Suits to all players this week. Here's where you can buy your green or purple alien suit without paying any money.

Alex, Anna Marie, Jon, and Chris bring you the latest in RPG news. I’m sorry, you’re out of energy. To listen to more, please use...

Paris show features wildlife costumes to emphasise the label’s planet-friendly ethos

The singer Janelle Monáe and actor Shailene Woodley were in the front row, but two rabbits, a fox, a horse, two cows and a crocodile stole the show. People in lifesize animal costumes, of the kind more usually seen at theme park parades than at Paris fashion week, joined models for the finale of Stella McCartney’s show, swinging their new-season handbags and posing for the cameras.

The optics were fun, but the message was serious – that there are animals on almost every catwalk, it’s just that they are usually dead. The half-moon shoulder bag carried jauntily by a brown cow here was made from a vegan alternative to leather, while other bags were created from second-life plastic.

As novel coronavirus spreads throughout the United States, millions of Americans are spending more time at home.MORE: Here's everything coming to Disney+ in AprilBut whether you're doing so because of a job loss, working from home situation or otherwise taking part in the mass effort to stay safe, chances are you've been bored once or twice while living under quarantine.Thankfully, some very talented people have been creating extra-special performances and experiences that you can enjoy to help you cope with the new normal and that don't break any social distancing rules. ...

2020 and 2021 will be one of the periodic transitional eras in gaming as Sony and Microsoft debut their shiny new consoles, the PlayStation 5 and Xbox Series X. To ease the process (and spur adoption of the next generation), EA may make its upcoming titles free to “upgrade” to your chosen console. On an […]

A nice touch during a difficult time

Justin Bieber discussed his experiences during the latest episode of his and wife Hailey's Facebook Watch series, The Biebers on Watch.

LOOKING to play a daily crossword online for free? We have great news - you can now play our world-famous Express Crusader Crossword puzzle every day, for free, right here.

MIKE GRAHAM furiously lashed out at journalist and author Christopher Snowdon live on-air after the author claimed the country has "never been less free" than it is today under the nationwide lockdown.

Follow our LIVE updates about the coronavirus outbreak here Coronavirus: The symptoms

A man has been charged with fraud after allegedly spending six free nights at a hotel by claiming to be an NHS worker.

Follow our live coronavirus updates HERE Coronavirus: the symptoms

Tiger King star Joe Exotic would seek "revenge" if he was released from prison, his former TV producer has claimed.

Coronavirus: the symptoms Read our LIVE updates on the coronavirus here

Follow our live Covid-19 updates HERE Coronavirus: The symptoms

Follow our live coronavirus updates here Coronavirus: the symptoms

Follow our live coronavirus updates HERE Coronavirus: The symptoms

The head of the World Health Organisation has brushed off calls for his resignation as he told the US to reconsider freezing its funding.

A five-star luxury hotel in London has been spreading a little joy amid the coronavirus outbreak by serving up free meals for key workers.

The Nightingale field hospital should take coronavirus patients from other London hospitals to enable them to become "Covid-free" and restart normal treatment, its chief executive has suggested.

A new biography of Harry and Meghan will "go beyond the headlines" and "reveal unknown details" of the couple's life together.

Union bosses have written to Sadiq Khan to demand a reversal of Transport for London's "disgraceful" decision to deny free travel to London Underground's cleaners.

But it was his reason for trying to make the 12-hour trip to California that shocked officers the most.

A five-year-old boy who was caught behind the wheel of his parents' car "driving to California to buy a Lamborghini" has been given a ride in his dream car by a kind stranger.

Airbnb CEO Brian Chesky described the global pandemic as the "most harrowing crisis of our lifetime" and said the coronavirus has cut the company's anticipated revenue in more than half.