You asked, we answered

This feed is dead. Stop polling it!

Getting the perfect shot requires great timing, the right equipment and nerves of steel

Michelle Frankfurter tells the stories of these young migrants and also those of the thousands who jump aboard “the death train”

From swirling aurora borealis to bioluminescent beaches, these award-winning photographs capture rare views of our world at night

"Fascinating, Captain"

The surge of hungry four-legged visitors to Belushya Guba is part of a larger trend

In one reef, three million sea cucumbers released 64,000 metric tons of nutrient-packed poo back into the ecosystem

As of last Wednesday, at least 3,500 sea turtles have been rescued from freezing waters in the midst record-breaking winter storm

In a twist befitting its pages, the satirical, anti-establishment publication that delivered laughs and hijinks to generations of young readers gets the respect it always deserved with a new museum exhibition

Renaissance paintings, medieval archives, cloistered orchards—how one Italian scientist is uncovering secrets that could help combat a growing agricultural crisis

These images from the Smithsonian Magazine Photo Contest show how communities in Mexico and beyond mark Día de los Muertos.

The artificial waste could fertilize the ocean and sequester carbon

Adverse weather has cancelled ferry crossings between Newfoundland and Labrador and Nova Scotia heading into the long weekend.

Nellie Winters is 87. From the time she was a child, her hands and mind have been busy pursuing her love of Inuit art and craft.

The A.C. Hunter Public Library wants people in St. John’s to know that it’s open and accessible, despite how the entrance looks. The main staircase is under repair but there’s plenty of signs directing folks to other entrances. Still, librarians say people are judging this book by its cover and assuming the library is closed.

A Labrador foster parent and head of a group working to better the Labrador foster care system is stressing the importance of keeping Indigenous children with Indigenous parents in their Indigenous communities.

Pat White, an entrepreneur who launched EVAS Air and the Gander Flight Training, has died. He was 69 years old.

The 50th Christmas Craft Fair gathered over a hundred vendors and thousands of customers in Torbay over the weekend. One family business was inspired by the early years of the fair and a passion to keep traditional crafts in style.

Nurses' union president Yvette Coffey says more must be done to ensure young nurses stay in the province. Newfoundland and Labrador Health Services says work is underway to match students with job openings.

A researcher studying the rise of extremism in Atlantic Canada warns Ku Klux Klan Halloween costumes are just one example of an insidious effort by far-right groups to normalize hateful attitudes.

A Port Hawkesbury woman has long imagined a Remembrance Day with her great-uncle's war medals. It will happen this year for the first time thanks to some sleuthing and the kindness of her great-uncle's foster family.

As Week 3 of the provincial election campaign begins, there are questions about how Nova Scotia's major political parties plan to combat the rising number of opioid overdose deaths. 

The Montreal English Theatre Awards ceremony is being held this evening at Le Gesù theatre. For the community, it's a time to celebrate their achievements and resilience coming out of the pandemic, together.

The Société de transport de Montréal (STM) has established a non-urgent text line for transit users to signal security issues in the Metro network.

Shilei Du, 36, was charged Monday morning with the premeditated murder of his partner, Guangmei Ye, whose body was found Sunday in a residence on Fontenelle Street in Candiac, Que.

Loved ones, friends, and people from across the city have all come together to support each other at Pastel Rita, the café Jocelyn Després helped so many call home.

The City of Montreal wants to sell more land to community organizations, housing co-ops and other groups to build off-market housing. 

Ten years after their formation, the members of The Brooks discuss how they’ve evolved and how they view their collaboration.

Quebec provincial police have confirmed that the body found in a Montreal nature park on Oct. 30 was that of kidnapping victim and cryptocurrency influencer Kevin Mirshahi.

Mosquitoes take blood meals from a diverse range of host animals and their host associations vary by species. Characterizing these associations is an important element of the transmission dynamics of mosquito-vectored pathogens. To characterize mosquito host associations, various molecular techniques have been developed, which are collectively referred to as blood meal analysis. DNA barcoding has diverse biological applications and is well-suited to mosquito blood meal analysis. The standard DNA barcoding marker for animals is a 5' fragment of the cytochrome c oxidase I (COI) gene. A major advantage of this marker is its taxonomic coverage in DNA sequence reference databases, making it feasible to identify a wider range of mosquito host species than with any other gene. However, the COI gene contains high sequence variation at potential priming sites between vertebrate orders. Coupled with the need for primer sequences to be mismatched with mosquito priming sites so that annealing to mosquito DNA is inhibited, it can be difficult to design primers suitable for blood meal analysis applications. Several primers are available that perform well in mosquito blood meal analysis, annealing to priming sites for most vertebrate host taxa, but not to those of mosquitoes. Because priming site sequence variation among vertebrate taxa can cause amplification to fail, a hierarchical approach to DNA barcoding-based blood meal analysis can be applied. In such an approach, no single primer set is expected to be effective for 100% of potential host species. If amplification fails in the initial reaction, a subsequent reaction is attempted with primers that anneal to different priming sites, and so on, until amplification is successful.

Mosquito species vary in their host associations. Although some species are relative generalists, most specialize, to varying extents, on particular types of host animals. Mosquito host associations are among the most important factors that influence the transmission dynamics of mosquito-vectored pathogens, and understanding these associations can provide insight on how such pathogens move within ecosystems. Characterization of the host associations of mosquito species requires applying blood meal analysis to the largest possible sample size of mosquito blood meals. Processing large samples of mosquito blood meals can be time-consuming, especially when chain-termination sequencing is used, necessitating individual processing of each specimen. Various methods and commercially available kits and products are available for extracting DNA from mosquito blood meals. The hot sodium hydroxide and Tris (HotSHOT) method is a rapid and inexpensive method of DNA extraction that is compatible with the recovery of DNA from mosquito blood meals preserved on QIAcard Flinders Technology Associates (FTA) Classic Cards (FTA cards). FTA cards allow nucleic acids found in blood meals to be preserved easily, even in field conditions. DNA prepared using this method is suitable for polymerase chain reaction (PCR)-based blood meal analysis.

All PCR- and DNA-based blood meal analyses require host DNA from a mosquito blood meal to be effectively preserved between the time when the specimen is collected and the extraction of DNA. As soon as a mosquito ingests blood from a host animal, digestion of host cells and cellular components within the blood meal by enzymes in the mosquito midgut begins to degrade the host DNA templates that are the targets of polymerase chain reaction (PCR) amplification. Without effective preservation, host DNA is typically undetectable by PCR 48 h after feeding, because of digestion. Preservation methods for mosquito blood meals vary in their efficacy, and the logistics of fieldwork can limit the options for preservation of blood meals and maintenance of the integrity of host DNA. This protocol describes a method of blood meal preservation that is effective, convenient, and amenable to fieldwork in remote locations where cryopreservation at –20°C or –80°C may not be feasible. It uses a Flinders Technology Associates (FTA) card, which is a chemically treated card that lyses cells and allows nucleic acids to be preserved. This method is also expected to preserve the DNA or RNA of pathogens present within the engorged mosquito abdomen, including RNA viruses.

On this episode of Generation Anthropocene, we dive into one of the most underappreciated elements on the periodic table

As The Meg hits theaters, dive into what we really know about this chompy predator

The ancient burial structures, strikingly similar all across Europe and the mediterranean, have puzzled scientists and historians for centuries

The Northwest Territories Power Corporation expects that by the end of March it will have generated 72 per cent of power for the North Slave region using hydro, and 28 per cent from diesel. Last year, about half the region's power was from diesel because of low water levels.

Tammy Roberts, Home Base's executive director, said before these services launched, there was "no place for youth to go" other than the emergency room or to RCMP cells. 

With president-elect Donald Trump promising to drill in Alaska's Arctic National Wildlife Refuge, the Gwich'in are preparing to fight for the Porcupine caribou herd.

Wanita Johnson, who celebrates her birthday this weekend in Whitehorse, still has a sharp memory for how the Second World War helped shape her life.

Water quality guidelines for mercury are 0.02 micrograms per litre of water. Several results show that levels at four sites are far above that threshold. During the second week of October, one value close to the mine was more than seven times the acceptable level.

A Whitehorse homeowner has been charged in connection with an explosion last year that destroyed his home and killed a man in a neighbouring house. 

John Quirke stayed in the job for 25 years — with a front row seat to Nunavut's politics as he managed the operations of the assembly and acted as the principal adviser for MLAs on parliamentary procedure among other things. 

A man who fled from police, drove onto a walking trail, triggered a shelter-in-place advisory for the Chateau Nova hotel in Yellowknife and subsequently broke into a home was on bail at the time and under house arrest, police say.

Newspaper editor Horace Greeley unsuccessfully ran against incumbent Ulysses S. Grant in November 1872. Twenty-four days later, he died of unknown causes at a private mental health facility

In a new biopic starring Kate Winslet, Miller's many lives—as an artist, model, muse, cook and war correspondent—need little embellishment

Fifty years ago, on October 5, 1974, David Kunst completed the first verified circumnavigation of the globe on foot. Along the way, he met Princess Grace of Monaco, raised money for UNICEF and lost a brother to bandits

A new film revisits the 90 minutes before the first episode of "Saturday Night Live" premiered on NBC on October 11, 1975

As a child, the future president acquired a marine animal's skull, which became the first specimen in his natural history collection

When the U.S. Army massacred a Lakota village at Blue Water, dozens of plundered artifacts ended up in the Smithsonian. The unraveling of this long-buried atrocity is forging a path toward reconciliation