Over the last several years it has become clear that higher order assemblies on membranes, exemplified by signalosomes, are a paradigm for the regulation of many membrane signaling processes. We have recently combined two-color direct stochastic optical reconstruction microscopy (dSTORM) with the (Clus-DoC) algorithm that combines cluster detection and colocalization analysis to observe the organization of 5-lipoxygenase (5-LO) and 5-lipoxygenase–activating protein (FLAP) into higher order assemblies on the nuclear envelope of mast cells; these assemblies were linked to leukotriene (LT) C4 production. In this study we investigated whether higher order assemblies of 5-LO and FLAP included cytosolic phospholipase A2 (cPLA2) and were linked to LTB4 production in murine neutrophils. Using two- and three-color dSTORM supported by fluorescence lifetime imaging microscopy we identified higher order assemblies containing 40 molecules (median) (IQR: 23, 87) of 5-LO, and 53 molecules (62, 156) of FLAP monomer. 98 (18, 154) molecules of cPLA2 were clustered with 5-LO, and 77 (33, 114) molecules of cPLA2 were associated with FLAP. These assemblies were tightly linked to LTB4 formation. The activation-dependent close associations of cPLA2, FLAP, and 5-LO in higher order assemblies on the nuclear envelope support a model in which arachidonic acid is generated by cPLA2 in apposition to FLAP, facilitating its transfer to 5-LO to initiate LT synthesis.

This recipe was featured on Foodie Tuesday, a weekly segment with Raf Epstein on Drive, 774 ABC Melbourne, 3:30 PM, courtesy of Phillippa Grogan. Her new book is 'Phillippa's Home Baking.'

This recipe features on Foodie Tuesday, a weekly segment on 774 Drive with Raf Epstein, 3.30PM, courtesy of Phillippa Grogan. Phillippa's latest book is "Phillippa's Home Baking".

This recipe features on Foodie Tuesday, a weekly segment on 774 Drive at 3.30PM. It was shared by Phillippa Grogan, adapted from Phillippa's Home Baking written with Richard Cornish.

In much of the world, gender is viewed as binary: man or woman, each assigned characteristics and traits designated by biological sex. But that's not the case everywhere, says France Villarta. In a talk that's part cultural love letter, part history lesson, he details the legacy of gender fluidity and inclusivity in his native Philippines -- and emphasizes the universal beauty of all people, regardless of society's labels.

DEADLINE FOR ORAL PRESENTATIONS: FEB 15, 2020 On June 5-6 2020, Temple University will host the 10th Annual Interdisciplinary Symposium on Decision Neuroscience (ISDN) in Philadelphia, PA. This symposium is unique in that it brings together a range of constituencies involved in the use of neuroscience techniques to understand consumer decision making – world renowned […]

The post Annual Interdisciplinary Symposium on Decision Neuroscience (ISDN), Philadelphia, June 5-6, 2020 appeared first on Decision Science News.

Hopson became the interim superintendent in Shelby County, Tenn., in 2013 after the Memphis City School system merged with Shelby County schools. That merger then led six suburban communities to break away from the merged school system to form their own school districts.

Colts quarterback Philip Rivers is signed for the 2020 season and he hasn't made any decisions about whether he'll keep playing in 2021, but it looks like he knows what he'll be doing when he does hang up his helmet. Rivers said when he signed with the Colts that he looked forward to coaching his [more]

War films -- History and criticism.

Libraries -- Philosophy.

Graphic novels -- History and criticism.

Libraries -- Marketing.

Architecture -- Australia -- History -- 20th century.

Asbestos -- Australia.

Habiburahman, 1979-

Hutton, George Stewart, 1906-1984.

Immigrants -- Government policy -- United States.

19th century

Paris : V. Adrien Delahaye, 1880.

Erlangen : F. Enke, 1850.

Gottingen : Dieterich, 1843.

Wien : F. Tempsky, 1890.

Leipzig : Duncker & Humblot, 1868.

Leipzig : Arnold, 1883.

Breslau : F. Hirt, 1872.

Leipzig : Deuticke, 1906.

Wien : Kaulfuss Witwe, Prandel, 1844.

Berlin : H. Steinitz, 1894.

Berlin : A. Hirschwald, 1864.

Leipzig : F. Deuticke, 1896.

Leipzig : Franz Deuticke, 1897.

Berlin : T.C.F. Enslin, 1866.

Stuttgart : Kroner, 1904.

Hamburg : Voss, 1886.

Berlin : Karger, 1910.

Berlin : A. Hirschwald, 1877.

Basel : Schwabe, 1886.

Wien : Urban & Schwarzenberg, 1879.

Berlin : Hirschwald, 1887.

Budapest : Franklin-Verein, 1895.

Leipzig : W. Engelmann, 1862.

Berlin : A. Hirschwald, 1895.

Berlin : Hirschwald, 1876.

Berlin : Hirschwald, 1905.

Leipzig : Toeplitz & Deuticke, 1888.

Wien : L.W. Seidel, 1875.

Wiesbaden : J.F. Bergmann, 1887.