Singapore : Springer Nature Singapore : Imprint: Springer, 2024.

β-1,3-d-Glucan is a ubiquitous glucose polymer produced by plants, bacteria, and most fungi. It has been used as a diagnostic tool in patients with invasive mycoses via a highly-sensitive reagent consisting of the blood coagulation system of horseshoe crab. However, no method is currently available for measuring β-1,6-glucan, another primary β-glucan structure of fungal polysaccharides. Herein, we describe the development of an economical and highly-sensitive and specific assay for β-1,6-glucan using a modified recombinant endo-β-1,6-glucanase having diminished glucan hydrolase activity. The purified β-1,6-glucanase derivative bound to the β-1,6-glucan pustulan with a KD of 16.4 nm. We validated the specificity of this β-1,6-glucan probe by demonstrating its ability to detect cell wall β-1,6-glucan from both yeast and hyphal forms of the opportunistic fungal pathogen Candida albicans, without any detectable binding to glucan lacking the long β-1,6-glucan branch. We developed a sandwich ELISA-like assay with a low limit of quantification for pustulan (1.5 pg/ml), and we successfully employed this assay in the quantification of extracellular β-1,6-glucan released by >250 patient-derived strains of different Candida species (including Candida auris) in culture supernatant in vitro. We also used this assay to measure β-1,6-glucan in vivo in the serum and in several organs in a mouse model of systemic candidiasis. Our work describes a reliable method for β-1,6-glucan detection, which may prove useful for the diagnosis of invasive fungal infections.

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Water resources allocation determines who is able to use water resources, how, when and where. Capturing information from 27 OECD countries and key partner economies, the report presents key findings from the OECD Survey of Water Resources Allocation and case studies of successful allocation reform.

Water resources allocation determines who is able to use water resources, how, when and where. Capturing information from 27 OECD countries and key partner economies, the report presents key findings from the OECD Survey of Water Resources Allocation and case studies of successful allocation reform.

This report provides a framework for policy discussions around financing water resources management that are taking place at local, basin, national, or transboundary levels.

Worldwide, 62 billion tons of natural resources – minerals, wood, metals, fossil and biomass fuels, and construction material – are extracted. On average, that’s almost 10 tons for every person on the earth. Of that, about one fifth ends up as waste and must be reused, recycled or disposed of in a way that is safe for people and the environment.

For World Environment Day on 5 June 2014, the OECD Environment Directorate looks at how we use and manage natural resources.

Water resources allocation determines who is able to use water resources, how, when and where. Capturing information from 27 OECD countries and key partner economies, the report presents key findings from the OECD Survey of Water Resources Allocation and case studies of successful allocation reform.

Advanced economies have reduced their consumption of raw materials and improved waste management, but more should be done to design and produce goods in a way that uses fewer natural resources and produces less waste, according to a new OECD report.

Worldwide, 62 billion tons of natural resources – minerals, wood, metals, fossil and biomass fuels, and construction material – are extracted. On average, that’s almost 10 tons for every person on the earth. Of that, about one fifth ends up as waste and must be reused, recycled or disposed of in a way that is safe for people and the environment.

Natural resources provide essential raw materials and other commodities, and are an important source of income and jobs. They also support the provision of ecosystem services necessary to develop human and social capital.

Despite its importance, regular measurement of the value of natural resources at national level is still in its infancy and often disconnected from valuation approaches for other assets.

Advanced economies have reduced their consumption of raw materials and improved waste management, but more should be done to design and produce goods in a way that uses fewer natural resources and produces less waste, according to a new OECD report.

These country notes profile public sector human resource practices and policies, covering issues including legal frameworks; age and gender composition of workers; public sector restructuring; management practices; industrial relations and reforms.

The seminar was organised by IPRASE, provincial institute for research and educational experimentation, instrumental body of the Autonomous Province of Trento, the Autonomous Province of Trento and the OECD LEED Trento Centre. The seminar represented a first public reflection on the Trentino Multilingualism Plan within a national and international comparison framework, in view of future prospects.

The United Kingdom has significantly boosted its foreign bribery enforcement efforts but needs to be more transparent when resolving cases.

Fighting foreign bribery is not a priority in Belgium. Together with the flagrant lack of resources for Belgian law enforcement authorities, this has resulted in very few foreign bribery investigations and prosecutions. In the 14 years since the entry into force of the foreign bribery offence in Belgium, only one case of bribery of foreign public officials has been concluded.

Ireland should increase its resources to detect and investigate foreign bribery more efficiently. Resources have, in recent years, been largely devoted to investigating non-bribery cases in the financial sector. Ireland has not prosecuted a foreign bribery case in the twelve years since its foreign bribery offence came into force, and law enforcement has taken few proactive steps to investigate allegations.

Technology has indeed entered the classroom; but it has not yet changed the ways we teach and learn to the same extent that it has transformed our way of communicating in the outside world.

Disadvantaged students don’t have as many resources at home as their advantaged peers so ideally schools would need to compensate by providing more support. However, often schools reinforce social disparities rather than moderate them.

The seminar was organised by IPRASE, provincial institute for research and educational experimentation, instrumental body of the Autonomous Province of Trento, the Autonomous Province of Trento and the OECD LEED Trento Centre. The seminar represented a first public reflection on the Trentino Multilingualism Plan within a national and international comparison framework, in view of future prospects.

Fears for prevention and treatment of other illnesses such as malaria and dengue fever

Xian Hu, Salma Jalal, Michael Sheetz, Oddmund Bakke, and Felix Margadant

Despite progress made in confocal microscopy, even fast systems still have insufficient temporal resolution for detailed live cell volume imaging, such as tracking rapid movement of membrane vesicles in three-dimensional space. Depending on the shortfall, this may result in undersampling and/or motion artifacts that ultimately limit the quality of the imaging data. By sacrificing detailed information in the Z-direction, we propose a new imaging modality that involves capturing fast "projections" from the field of depth which shortens imaging time by approximately an order of magnitude as compared to standard volumetric confocal imaging. With faster imaging, radiation exposure to the sample is reduced, resulting in less fluorophore photobleaching and potential photodamage. The implementation minimally requires two synchronized control signals that drive a piezo stage and trigger the camera exposure. The device generating the signals has been tested on spinning disk confocals and instant structured-illumination-microscopy (iSIM) microscopes. Our calibration images show that the approach provides highly repeatable and stable imaging conditions that enable photometric measurements of the acquired data, in both standard live imaging and super-resolution modes.

Björn Eismann, Teresa G. Krieger, Jürgen Beneke, Ruben Bulkescher, Lukas Adam, Holger Erfle, Carl Herrmann, Roland Eils, and Christian Conrad

3D cell cultures enable the in vitro study of dynamic biological processes such as the cell cycle, but their use in high-throughput screens remains impractical with conventional fluorescent microscopy. Here, we present a screening workflow for the automated evaluation of mitotic phenotypes in 3D cell cultures by light-sheet microscopy. After sample preparation by a liquid handling robot, cell spheroids are imaged for 24 hours in toto with a dual-view inverted selective plane illumination microscope (diSPIM) with a much improved signal-to-noise ratio, higher imaging speed, isotropic resolution and reduced light exposure compared to a spinning disc confocal microscope. A dedicated high-content image processing pipeline implements convolutional neural network based phenotype classification. We illustrate the potential of our approach by siRNA knock-down and epigenetic modification of 28 mitotic target genes for assessing their phenotypic role in mitosis. By rendering light-sheet microscopy operational for high-throughput screening applications, this workflow enables target gene characterization or drug candidate evaluation in tissue-like 3D cell culture models.

Here are some of the many resources the Smithsonian Libraries have to offer for Exploration, Encounter, Exchange in History

The post On-line resources for Smithsonian Libraries appeared first on Smithsonian Insider.

With the recent 22% rise in the price of uranium, Peter Epstein of Epstein Research considers the upside for Skyharbour's holdings in the Athabasca Basin.

With the recent 22% rise in the price of uranium, Peter Epstein of Epstein Research considers the upside for Skyharbour's holdings in the Athabasca Basin.

Under pressure from rising food prices, many nations have begun to acquire large tracts of agricultural land in foreign countries, a practice known as ‘land grabbing’. New research has now quantified current levels of land grabbing and demonstrated that it is accompanied by concerning levels of ‘water grabbing’ which could affect water supply in the ‘grabbed’ countries.

The ability of the Earth’s living systems to store carbon could play a vital role in the mitigation of climate change. A new report suggests that, in coming decades, safeguarding and restoring carbon in ecosystems has the potential to prevent well over 50 gigatonnes (Gt) of carbon entering the atmosphere.

A recent study has investigated how taxes on virgin raw materials used in construction, such as gravel and sand, have reduced the use of these resources, based on experiences in Denmark, Sweden and the UK. However, greater incentives to recycle these materials are still needed, says the study.

A recent study has found that improved management of phosphorus in the EU would reduce reliance on imported phosphorus, in addition to reducing damage from excess phosphorus in the environment. This could be achieved through the appropriate use of fertilisers and greater recovery and recycling of phosphorus from all waste sources.

In the Mediterranean region the demand for natural resources and ecological services is two and half times greater than ecosystems’ capacity to provide them, recent research has found. To meet this demand, countries rely on imports, exposing themselves to price volatility and potential resource shortages. According to the authors, a 10% increase in global prices would particularly impact vulnerable countries such as Jordan, which would see its trade balance worsening by 2.4% of its gross domestic product.

New research highlights that potential to use forest materials as bioenergy to reduce greenhouse gas (GHG) emissions from fossil fuel use must be balanced against the role played by forest stocks as carbon storage facilities.

Ambiguity arises in a decision-making process when there is more than one way of interpreting a problematic situation. A new study has explored five possible strategies to overcome ambiguity in natural resources management – rational problem solving, persuasive communication, learning through dialogue, negotiation and opposition. A case study of water management in Spain is given to illustrate how these strategies have been used.

Researchers have developed a ‘watershed economics approach’ which could help water managers balance competing uses for water and understand the impact of different policies. To demonstrate the approach, it was applied to a watershed in Cyprus.

A new study has concluded that the benefits of carbon capture and storage (CCS) to human health and ecosystems from reduced climate change related impacts considerably outweigh any negative impacts from using the technology in power plants. However, CCS has a large impact on the depletion of natural resources.

Under pressure from rising food prices, many nations have begun to acquire large tracts of agricultural land in foreign countries, a practice known as ‘land grabbing’. New research has now quantified current levels of land grabbing and demonstrated that it is accompanied by concerning levels of ‘water grabbing’ which could affect water supply in the ‘grabbed’ countries.

Population growth of any country is ultimately dependent on that country’s access to freshwater resources, which also determines its capacity to produce food. A new study shows that population growth is expected to decline in countries with low water resources, as water-rich countries reduce food exports in order to feed their own growing populations.

Water resource management needs to adapt to changes in climate, water demand and land use. A new tool has been developed by the LIFE+ Water Change Project to assess these ‘global change’ impacts on water resources and inform decisions on optimal adaptation strategies. A recent study has applied the tool to a river basin in Spain.

Dense urban environments have significant resource-saving potential and serve as good platforms for climate change mitigation. This study reviewed an initiative to improve use of energy and water in Rotterdam, highlighting factors important for success including exchanges in close geographic proximity and private-sector participation.

Water-supply planning that considers the preferences of multiple stakeholders under uncertain and variable future conditions are more robust than planning decisions based on historical conditions, a recent study has stated. Using the Thames river basin in the UK as an example, the researchers present a new computer-modelling approach to assess which combinations of water-management measures best secure future water supply under a wide range of possible future conditions.

Scientists have published findings from the largest and broadest survey on sewage resource recovery conducted in Europe to date. Researchers surveyed more than 600 waste-water treatment plants (WWTPs) in Italy, which represent approximately 25% of the country’s total load of treated sewage. The findings provide a comprehensive picture of the current state of sewage resource recovery in WWTPs, revealing that just 40% of plants perform some form of material or energy recovery, and identifying several of the important driving forces behind implementation. This research provides valuable information and insights for policymakers and WWTP managers aiming to improve WWTP sustainability and close the sewage resource-recovery loop.

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Designing the best defense against infection was our only way forward.

Melissa Harville-Lebron, CEO of E2 Northeast Motorsports has inked a deal with NASCAR Camping World Truck Series (NCWTS) team, Copp Motorsports and is set to field Florida-based driver, Scott Stenzel in the NextEra Energy Resources 250.