The way dogs are portrayed in literature reflects the problems in our real-world relationships to canines, says PhD student Molly Labenski, who says people want animals in their lives as “accessories” that can be easily discarded.

Animals — what on earth are they thinking? A panel of scientists explore the notion of animal cognition from what your dog means when it wags its tail, to the incredible problem-solving skills of crows, as part of the Aspen Ideas Festival.

“Chalk Talk” returns to talk about student health, school bonds and more. A new analysis looks at California’s campaign watchdog agency. Finally, Sacramento ghost guides talk about some of their favorite supernatural tales.

While your pooch may not be able to carve the gnar, he or she would probably love to join you on your next ski vacation, eating snow, chilling by the fire and eating wagyu beef.

Yes, man's best friend can benefit from CBD, too.

Fashion house Dolce & Gabbana has launched a new alcohol-free perfume for dogs called "Fefé" in honor of Domenico Dolce’s poodle, but not all vets and pet owners agree it’s safe or appropriate.

Flying never felt this cool.

Video of the shocking incident went viral.

The Bolles School girls’ swimming team, which includes Marleigh Howes, captured the 2024 Bulldog Byron Plapp Invite Women’s Division title with a total of 811 points. Howes contributed to the team’s success with several strong individual performances. She placed 8th in the 200-yard freestyle preliminaries (2:02.75) and improved her time to 2:01.56 in the A-final, […]

Tuff Dogs and Robin Hood recorded wins in the Corona League at Goose Gosling Field on Friday [November 1]. Tuff Dogs defeated YGSFC 2-1 thanks to goals from Miguel Cabral and Jao Madeiros. Rico Wells scored for YGSFC. Preston Farrow scored the only goal of the game in Hood’s 1-0 win over Terrace Onions. InterBDA’s […]

Gabriella Cechini won the Varsity A and AA Women’s 5K 2024 Ben Bloser Bulldog XC Invitational in West Virginia. Cechini –  representing Mercersburg Academy — clocked a winning time of 19:01.9, helping the girls team to finish 3rd with a combined time of 1:49:28. Related Stories Armstrong Competes In MAAC Championships Marshall & Outerbridge Compete […]

Today my family is getting out this stuff they call Christmas decorations and I'm helping. There's even a little tree just my size in the room where I sleep!



It's kind of exciting, but this decorating thing can make a girl tired!
-Grace

 This weekend I dressed up and went on a walk with all my friends incognito.

The final day of TIFF 2020 has come and gone and below are my final capsule reviews. I’ll post a full capsule roundup on Monday.

Fauna [Mexico/Canada, Nicolás Pereda, 3.5] Narratives nest within narratives when an actor visits his girlfriend’s family in a sleepy small town. Comic misunderstandings, naturalistic locations and twisting meta-story may remind seasoned festival-goers of the works of Hong Sang-soo, with Coronas instead of soju.

Preparations to Be Together For an Unknown Period of Time [Hungary, Lili Horvát, 4] Top neurologist questions the accuracy of her recollections when she moves back home from the US to Budapest for a romantic rendezvous, only to find that the object of her affections professes not to remember her. Quietly suspenseful drama of psychological uncertainty.

The Truffle Hunters [Italy, Michael Dweck & Gregory Kershaw, 4] An aging generation of Piedmontese truffle hunters carries on the search for the elusive delicacy, fearing the poison bait left for their beloved dogs by ruthless newcomers to the trade. A documentary balm for lovers of food and canines luxuriates in the presence of sumptuously photographed forest eccentrics and their very, very good dogs.

Bandar Band [Iran/Germany, Manijeh Hekmat, 3] A pregnant singer, her husband and their guitarist try to get their van through a floodstruck region to attend a contest gig in Tehran. Neorealist drama where the obstacles in the characters’ path are literal.

The Water Man [US, David Oyelowo, 3.5] Imaginative kid (Lonnie Chavis) heads into the Northwestern forest in search of a legendary immortal, thinking he holds the secret to curing his mom (Rosario Dawson) of leukemia. One of the more successful of a recent wave of films that put a somber sin on 80s kids adventure, thanks to a well-constructed script and Oyelowo’s sure control of tone.

Among the differences of this digital-only fest was that it removed the flexibility to choose between multiple screening dates. In a regular year I program the last days and work backward to end on some combination of stronger and/or lighter selections. Here programmers assigned a 24 hour window for each film. These last movies weren’t what I would have picked as closers in ordinary times. To compensate for this Valerie and I are running a day of fake TIFF programming to simulate the funner final Sunday we usually shoot for. They consist of one film that played at TIFF 2019 and three others from previously-appearing directors. Play along at home by streaming The Vast of Night, The Forest of Love*, Mr. & Mrs. Adelman, and Ace Attorney.

*Update: Turns out this one is ultra-disturbing and in no way fun or light. Going into something with mistaken tonal expectations—just like the real TIFF!

There was a news item this week about the sentencing of some people who organised dog fights, with large sums wagered on the result. I saw one such case a few years ago, and it needed a strong stomach to look at the evidence. The fight took place in an abandoned farm building and at the end the whitewashed walls were heavily bloodstained. We simply remanded the two defendants, and my colleagues sentenced them a few weeks later after reports were prepared. The aggravation was considerable; organised for money, dogs had to be destroyed, and so on so. They received the maximum six months each and were banned from keeping animals for ten years. In this latest case numerous social-media comments have complained that the six month sentence was not enough, but as usual that raises the question of just how long is enough? All sentences have to fit into the scale somewhere; for example can it ever be right to impose a higher penalty for cruelty to animals than to people?

Here's the Guideline:-

http://www.sentencingcouncil.org.uk/offences/item/animal-cruelty/

Dogecoin shot higher on Tuesday night, extending its postelection surge after President-elect Donald Trump formally announced the creation of the Department of Government Efficiency, which he referred to as "DOGE" in his statement. Tesla CEO Elon Musk and Vivek Ramaswamy, former Republican…

Investing.com-- Bitcoin fell from record highs on Wednesday, retreating as optimism over a Donald Trump presidency now appeared to be cooling, with focus turning to upcoming U.S. inflation data for more cues. Major meme token Dogecoin also retreated on Wednesday after a stellar run-up to…

In an X post, Musk said that all Department of Government Efficiency actions will be posted online for maximum transparency.

DOGE trades at premium on Upbit and Bithumb relative to Binance. - The price differential is still noticeably lower than the previous bull market peaks. Today is just that day as Koreans seem to be jumping into the DOGE market, driving prices higher and creating a noticeable premium on local…

President-elect Donald Trump, on Tuesday, greenlit the formation of the Department of Government Efficiency or DOGE, the acronym which almost sounds like a parody of the cryptocurrency known as Dogecoin (CRYPTO: DOGE). As the department promises to restructure federal agencies and reduce…

When he wrote this letter to his mother in 1944, Roald Dahl was working at the British Embassy in Washington, D.C.—one of hundreds of undercover agents employed by Britain’s MI6 foreign intelligence service to spy on the United States. Dahl’s debut novel, The Gremlins, had been published the year before, and it would be another

Sleeping Dogs™ Definitive Edition (Xbox Series/One) 

All recommended by dog lovers. READ MORE...

In the IKEAHackers Facebook Group, Brianna shared how she turned basic KALLAX units into something totally unexpected — a gorgeous credenza that includes a perfect spot for her dog to lounge. Brianna took two 2×4 KALLAX units and set them up on either end of the nook. The middle section would be left open for a dog bed. But she didn’t stop at just placing the units – she snuck in a little hacking to give the credenza an upgraded […]

The post This IKEA KALLAX Hack Combines Storage and a Cozy Dog Bed in the Coolest Way appeared first on IKEA Hackers.

Sarah says This is our female prairie dog, Lily. She loves her triscuits!

I’m at a briefing with a client, along with a board made up of the client’s in-house developers and designers. While discussing an idea with some of the other people in the room, the client stands up and starts hitting the desk with the newspaper.

Me: “Excuse me, but what are you doing?”

Client: “Hush, this works on my dogs at home. It should work on you too.”

He beats the table with the newspaper for a few seconds.

Client: “Now, sit, and stay.”

He continues to beat the table. 

Me: “I’m sorry, this is ridiculous. I’m terminating you as a client.”

As I walked towards the door, the client jumped up from their seat and blocked the door.

Client: “If you want a snack, I’ve got some under my desk, no need to leave the meeting!”

At this point, I shoved him aside, and walked out, followed by him. Whistling, and shouting “come!”

The post One Day of a Flying Dog appeared first on English Russia.

Dogs can sniff out disease and analyze new odors even as they exhale. But how?

It’s surprisingly common for dogs to eat poop, a habit called coprophagia.

By David Blackmon In the wake of the election of President Donald Trump to serve a second term in office, along with presumptive Republican majorities in both houses of Congress, many are now asking about what the future will hold for the oddly named Inflation Reduction Act. Trump made it repeatedly clear on the campaign […]

The post Biden’s Signature Climate ‘Boondoggle’ Might Be On Chopping Block After Trump Win appeared first on Liberty Unyielding.

Sal Naseem tells the BBC he wasn't convinced Mr Kaba presented a sufficient danger to justify being shot.

Nineteen years and 10 miles separated Zambia's football team from a lost, golden generation on a fateful night in Libreville.

Some things you should never do on a dog walk include approaching unfamiliar pets, using an extendable leash, and not picking up waste, experts said.

Dogecoin has experienced a significant surge in value since last month, with impressive price gains continuing today as it trades… Continue reading Dogecoin at the Edge of a Major Breakout – Could $2 Be Possible with Bitcoin Dominance Holding Strong?

The post Dogecoin at the Edge of a Major Breakout – Could $2 Be Possible with Bitcoin Dominance Holding Strong? appeared first on ReadWrite.

A Minnessota maker named Caleb Olson built the Poopcopter, an autonomous drone that seeks out dog-doo and retrieves it for disposal.

He describes it as the "world's first aerial bound self-guided dog poop removal system." See it in action below.

"The Poopcopter is capable of scanning areas defined by a user, your backyard for example, and as it scans it's performing real-time computer vision using the camera which is inside the drone," Olson says. — Read the rest

The post The Poopcopter is a DIY autonomous drone that finds and collects dog-doo appeared first on Boing Boing.

I don’t know how old our dog Posey is. She’s a rescue pup that we found at the city shelter back in 2011. We took her home, somewhat unexpectedly, that same day. It was Super Bowl Sunday, so every year at about that time we celebrate (our oh-so-creatively-named) birthday alternative, “Dog Day,” by taking her […]

The article Dog Day & Donuts in Half Moon Bay, CA originated at EverInTransit.com

PROMOTED RACING, CHAPELTON MAROONS RENEW RIVALRY IN JPL - A16 FUNCAANDUN SET FOR FIFTH STRAIGHT WIN - A21

Clostridium difficile is an anaerobic and spore-forming bacterium responsible for 15–25% of postantibiotic diarrhea and 95% of pseudomembranous colitis. Peptidoglycan is a crucial element of the bacterial cell wall that is exposed to the host, making it an important target for the innate immune system. The C. difficile peptidoglycan is largely N-deacetylated on its glucosamine (93% of muropeptides) through the activity of enzymes known as N-deacetylases, and this N-deacetylation modulates host–pathogen interactions, such as resistance to the bacteriolytic activity of lysozyme, virulence, and host innate immune responses. C. difficile genome analysis showed that 12 genes potentially encode N-deacetylases; however, which of these N-deacetylases are involved in peptidoglycan N-deacetylation remains unknown. Here, we report the enzymes responsible for peptidoglycan N-deacetylation and their respective regulation. Through peptidoglycan analysis of several mutants, we found that the N-deacetylases PdaV and PgdA act in synergy. Together they are responsible for the high level of peptidoglycan N-deacetylation in C. difficile and the consequent resistance to lysozyme. We also characterized a third enzyme, PgdB, as a glucosamine N-deacetylase. However, its impact on N-deacetylation and lysozyme resistance is limited, and its physiological role remains to be dissected. Finally, given the influence of peptidoglycan N-deacetylation on host defense against pathogens, we investigated the virulence and colonization ability of the mutants. Unlike what has been shown in other pathogenic bacteria, a lack of N-deacetylation in C. difficile is not linked to a decrease in virulence.

Broad-specificity glycoside hydrolases (GHs) contribute to plant biomass hydrolysis by degrading a diverse range of polysaccharides, making them useful catalysts for renewable energy and biocommodity production. Discovery of new GHs with improved kinetic parameters or more tolerant substrate-binding sites could increase the efficiency of renewable bioenergy production even further. GH5 has over 50 subfamilies exhibiting selectivities for reaction with β-(1,4)–linked oligo- and polysaccharides. Among these, subfamily 4 (GH5_4) contains numerous broad-selectivity endoglucanases that hydrolyze cellulose, xyloglucan, and mixed-linkage glucans. We previously surveyed the whole subfamily and found over 100 new broad-specificity endoglucanases, although the structural origins of broad specificity remained unclear. A mechanistic understanding of GH5_4 substrate specificity would help inform the best protein design strategies and the most appropriate industrial application of broad-specificity endoglucanases. Here we report structures of 10 new GH5_4 enzymes from cellulolytic microbes and characterize their substrate selectivity using normalized reducing sugar assays and MS. We found that GH5_4 enzymes have the highest catalytic efficiency for hydrolysis of xyloglucan, glucomannan, and soluble β-glucans, with opportunistic secondary reactions on cellulose, mannan, and xylan. The positions of key aromatic residues determine the overall reaction rate and breadth of substrate tolerance, and they contribute to differences in oligosaccharide cleavage patterns. Our new composite model identifies several critical structural features that confer broad specificity and may be readily engineered into existing industrial enzymes. We demonstrate that GH5_4 endoglucanases can have broad specificity without sacrificing high activity, making them a valuable addition to the biomass deconstruction toolset.

Peptidoglycan (PG) is an essential constituent of the bacterial cell wall. During cell division, the machinery responsible for PG synthesis localizes mid-cell, at the septum, under the control of a multiprotein complex called the divisome. In Escherichia coli, septal PG synthesis and cell constriction rely on the accumulation of FtsN at the division site. Interestingly, a short sequence of FtsN (Leu75–Gln93, known as EFtsN) was shown to be essential and sufficient for its functioning in vivo, but what exactly this sequence is doing remained unknown. Here, we show that EFtsN binds specifically to the major PG synthase PBP1b and is sufficient to stimulate its biosynthetic glycosyltransferase (GTase) activity. We also report the crystal structure of PBP1b in complex with EFtsN, which demonstrates that EFtsN binds at the junction between the GTase and UB2H domains of PBP1b. Interestingly, mutations to two residues (R141A/R397A) within the EFtsN-binding pocket reduced the activation of PBP1b by FtsN but not by the lipoprotein LpoB. This mutant was unable to rescue the ΔponB-ponAts strain, which lacks PBP1b and has a thermosensitive PBP1a, at nonpermissive temperature and induced a mild cell-chaining phenotype and cell lysis. Altogether, the results show that EFtsN interacts with PBP1b and that this interaction plays a role in the activation of its GTase activity by FtsN, which may contribute to the overall septal PG synthesis and regulation during cell division.

Oktawia Nilsson
Nov 17, 2020; 0:jlr.RA120000920v1-jlr.RA120000920
Research Articles

Apolipoprotein A-I (ApoA-I) of high-density lipoprotein (HDL) is essential for the transportation of cholesterol between peripheral tissues and the liver. However, specific mutations in Apolipoprotein A-I (ApoA-I) of high-density lipoprotein (HDL) are responsible for a late-onset systemic amyloidosis, the pathological accumulation of protein fibrils in tissues and organs. Carriers of these mutations do not exhibit increased cardiovascular disease risk despite displaying reduced levels of ApoA-I/ HDL-cholesterol. To explain this paradox, we show that the HDL particle profile of patients carrying either L75P or L174S ApoA-I amyloidogenic variants a higher relative abundance of the 8.4 nm vs 9.6 nm particles, and that serum from patients, as well as reconstituted 8.4 and 9.6 nm HDL particles (rHDL), possess increased capacity to catalyze cholesterol efflux from macrophages. Synchrotron radiation circular dichroism and hydrogen-deuterium exchange revealed that the variants in 8.4 nm rHDL have altered secondary structure composition and display a more flexible binding to lipids compared to their native counterpart. The reduced HDL-cholesterol levels of patients carrying ApoA-I amyloidogenic variants are thus balanced by higher proportion of small, dense HDL particles and better cholesterol efflux due to altered, region-specific protein structure dynamics.

Federal watchdogs find that Utah inflated its high school graduation rate in the last of a series of reports warning states not to make end runs around the rules for calculating graduation rates.