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Proteomics of the Chloroplast Envelope Membranes from Arabidopsis thaliana

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May 1, 2003; 2:325-345
Research




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Mass Spectrometry of Human Leukocyte Antigen Class I Peptidomes Reveals Strong Effects of Protein Abundance and Turnover on Antigen Presentation

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A PP2A Phosphatase High Density Interaction Network Identifies a Novel Striatin-interacting Phosphatase and Kinase Complex Linked to the Cerebral Cavernous Malformation 3 (CCM3) Protein

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Analysis of the Subunit Composition of Complex I from Bovine Heart Mitochondria

Joe Carroll
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Distinct and Overlapping Sets of SUMO-1 and SUMO-2 Target Proteins Revealed by Quantitative Proteomics

Alfred C. O. Vertegaal
Dec 1, 2006; 5:2298-2310
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Lysine Acetylation Is a Highly Abundant and Evolutionarily Conserved Modification in Escherichia Coli

Junmei Zhang
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Extending the Limits of Quantitative Proteome Profiling with Data-Independent Acquisition and Application to Acetaminophen-Treated Three-Dimensional Liver Microtissues

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Parallel Reaction Monitoring for High Resolution and High Mass Accuracy Quantitative, Targeted Proteomics

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Technological Innovation and Resources




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Lysine Propionylation and Butyrylation Are Novel Post-translational Modifications in Histones

Yue Chen
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Phosphoproteome Analysis of E. coli Reveals Evolutionary Conservation of Bacterial Ser/Thr/Tyr Phosphorylation

Boris Macek
Feb 1, 2008; 7:299-307
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High Resolution Clear Native Electrophoresis for In-gel Functional Assays and Fluorescence Studies of Membrane Protein Complexes

Ilka Wittig
Jul 1, 2007; 6:1215-1225
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In Vivo Identification of Human Small Ubiquitin-like Modifier Polymerization Sites by High Accuracy Mass Spectrometry and an in Vitro to in Vivo Strategy

Ivan Matic
Jan 1, 2008; 7:132-144
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Fluorescent Proteins as Proteomic Probes

Ileana M. Cristea
Dec 1, 2005; 4:1933-1941
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PaxDb, a Database of Protein Abundance Averages Across All Three Domains of Life

M. Wang
Aug 1, 2012; 11:492-500
Technological Innovation and Resources




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Global Identification and Characterization of Both O-GlcNAcylation and Phosphorylation at the Murine Synapse

Jonathan C. Trinidad
Aug 1, 2012; 11:215-229
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Large Scale Screening for Novel Rab Effectors Reveals Unexpected Broad Rab Binding Specificity

Mitsunori Fukuda
Jun 1, 2008; 7:1031-1042
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Quantitative, Multiplexed Assays for Low Abundance Proteins in Plasma by Targeted Mass Spectrometry and Stable Isotope Dilution

Hasmik Keshishian
Dec 1, 2007; 6:2212-2229
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The Proteome of the Mouse Photoreceptor Sensory Cilium Complex

Qin Liu
Aug 1, 2007; 6:1299-1317
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Comparative Proteomic Analysis of Eleven Common Cell Lines Reveals Ubiquitous but Varying Expression of Most Proteins

Tamar Geiger
Mar 1, 2012; 11:M111.014050-M111.014050
Special Issue: Prospects in Space and Time




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Time-resolved Mass Spectrometry of Tyrosine Phosphorylation Sites in the Epidermal Growth Factor Receptor Signaling Network Reveals Dynamic Modules

Yi Zhang
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Complementary Profiling of Gene Expression at the Transcriptome and Proteome Levels in Saccharomyces cerevisiae

Timothy J. Griffin
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A Proteomic Analysis of Human Cilia: Identification of Novel Components

Lawrence E. Ostrowski
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Toward a Comprehensive Atlas of the Physical Interactome of Saccharomyces cerevisiae

Sean R. Collins
Mar 1, 2007; 6:439-450
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A Proteome-wide, Quantitative Survey of In Vivo Ubiquitylation Sites Reveals Widespread Regulatory Roles

Sebastian A. Wagner
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A Tandem Affinity Tag for Two-step Purification under Fully Denaturing Conditions: Application in Ubiquitin Profiling and Protein Complex Identification Combined with in vivoCross-Linking

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Quantitative Phosphoproteomics of Early Elicitor Signaling in Arabidopsis

Joris J. Benschop
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Discordant Protein and mRNA Expression in Lung Adenocarcinomas

Guoan Chen
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Integrated Genomic and Proteomic Analyses of Gene Expression in Mammalian Cells

Qiang Tian
Oct 1, 2004; 3:960-969
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Trypsin Cleaves Exclusively C-terminal to Arginine and Lysine Residues

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Interpretation of Shotgun Proteomic Data: The Protein Inference Problem

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Targeted Data Extraction of the MS/MS Spectra Generated by Data-independent Acquisition: A New Concept for Consistent and Accurate Proteome Analysis

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GPS 2.0, a Tool to Predict Kinase-specific Phosphorylation Sites in Hierarchy

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A Multidimensional Chromatography Technology for In-depth Phosphoproteome Analysis

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A Human Protein Atlas for Normal and Cancer Tissues Based on Antibody Proteomics

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Comparison of Label-free Methods for Quantifying Human Proteins by Shotgun Proteomics

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Quantitative Mass Spectrometric Multiple Reaction Monitoring Assays for Major Plasma Proteins

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The Paragon Algorithm, a Next Generation Search Engine That Uses Sequence Temperature Values and Feature Probabilities to Identify Peptides from Tandem Mass Spectra

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Sep 1, 2007; 6:1638-1655
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Quantitative Phosphoproteomics Applied to the Yeast Pheromone Signaling Pathway

Albrecht Gruhler
Mar 1, 2005; 4:310-327
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Highly Selective Enrichment of Phosphorylated Peptides from Peptide Mixtures Using Titanium Dioxide Microcolumns

Martin R. Larsen
Jul 1, 2005; 4:873-886
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Absolute Quantification of Proteins by LCMSE: A Virtue of Parallel ms Acquisition

Jeffrey C. Silva
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The Human Plasma Proteome: History, Character, and Diagnostic Prospects

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Analysis of the Human Tissue-specific Expression by Genome-wide Integration of Transcriptomics and Antibody-based Proteomics

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A Versatile Nanotrap for Biochemical and Functional Studies with Fluorescent Fusion Proteins

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Exponentially Modified Protein Abundance Index (emPAI) for Estimation of Absolute Protein Amount in Proteomics by the Number of Sequenced Peptides per Protein

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Parts per Million Mass Accuracy on an Orbitrap Mass Spectrometer via Lock Mass Injection into a C-trap

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Dec 1, 2005; 4:2010-2021
Technology




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Phosphate-binding Tag, a New Tool to Visualize Phosphorylated Proteins

Eiji Kinoshita
Apr 1, 2006; 5:749-757
Technology




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Multiplexed Protein Quantitation in Saccharomyces cerevisiae Using Amine-reactive Isobaric Tagging Reagents

Philip L. Ross
Dec 1, 2004; 3:1154-1169
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Sep 1, 2014; 13:2513-2526
Technological Innovation and Resources




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Stable Isotope Labeling by Amino Acids in Cell Culture, SILAC, as a Simple and Accurate Approach to Expression Proteomics

Shao-En Ong
May 1, 2002; 1:376-386
Research




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Reply to Cosgrove: Non-enzymatic action of expansins [Letters to the Editor]

In our computational study, we use molecular simulations to substantiate a hypothetical mechanism for glycosidic bond cleavage in the presence of a single catalytic acid at the active site of the mutant D10N HiCel45A. In addition to discussing this plausible mechanism from the context of structurally related MltA lytic transglycosylase and subfamily C GH45s, we also suggest the implications of the plausible mechanism for our current understanding of the action of expansins and lytic transglycosylases. As correctly pointed out by Professor Cosgrove (1), there is large body of evidence, a significant portion of which was regrettably not discussed in our paper, that suggests that expansins are incapable of lytic action on polysaccharide substrates. Whereas these insights do not change the results or the conclusions of our article, we would like to thank Professor Cosgrove for these additional insights. In particular, our main point with respect to expansins is that our results suggest the possibility that expansins are capable of nonhydrolytic lytic activity. Our intention was not to suggest this was the mechanism of expansins, but that it should be considered based on our results and the similarity of the active sites.The molecular mechanisms of how expansins enable cell wall expansion remains to be fully understood. Whereas our proposed mechanism resulting in the formation of the 1,6-anhdro product might be found in expansins and might contribute to the mode of action of expansins, we would like to emphasize that the intent of this study was only to suggest this as a...