Samantha-Su Z. Taylor, Nicole L. Jacobsen, Tasha K. Pontifex, Paul Langlais, and Janis M. Burt

Connexin 37 (Cx37) expression profoundly suppresses proliferation of rat insulinoma (Rin) cells in a manner dependent on gap junction channel (GJCh) functionality and the presence and phosphorylation status of its carboxyl-terminus (CT). In Rin cells growth arrested by induced Cx37 expression, serine 319 (S319) is frequently phosphorylated. Preventing phosphorylation at this site (alanine substitution; S319A) relieved Cx37 of its growth suppressive effect whereas mimicking phosphorylation at this site (aspartate substitution; S319D) enhanced Cx37's growth suppressive properties. Like Cx37-WT, -S319D GJChs and hemichannels (HChs) preferred the closed state, rarely opening fully, and gated slowly. In contrast, Cx37-S319A channels preferred open states, opened fully, and gated rapidly. These data indicate that phosphorylation-dependent conformational differences in Cx37 protein and channel function underlie Cx37-induced growth arrest vs. growth permissive phenotypes. That the closed state of -WT and Cx37-S319D GJChs and HChs favors growth arrest suggests that rather than specific permeants mediating cell cycle arrest, the closed conformation instead supports interaction of Cx37 with growth regulatory proteins that result in growth arrest.

Crystal structure identification of thin organic films entails a number of technical and methodological challenges. In particular, if molecular crystals are epitaxially grown on single-crystalline substrates a complex scenario of multiple preferred orientations of the adsorbate, several symmetry-related in-plane alignments and the occurrence of unknown polymorphs is frequently observed. In theory, the parameters of the reduced unit cell and its orientation can simply be obtained from the matrix of three linearly independent reciprocal-space vectors. However, if the sample exhibits unit cells in various orientations and/or with different lattice parameters, it is necessary to assign all experimentally obtained reflections to their associated individual origin. In the present work, an effective algorithm is described to accomplish this task in order to determine the unit-cell parameters of complex systems comprising different orientations and polymorphs. This method is applied to a polycrystalline thin film of the conjugated organic material 6,13-pentacene­quinone (PQ) epitaxially grown on an Ag(111) surface. All reciprocal vectors can be allocated to unit cells of the same lattice constants but grown in various orientations [sixfold rotational symmetry for the contact planes (102) and (102)]. The as-determined unit cell is identical to that reported in a previous study determined for a fibre-textured PQ film. Preliminary results further indicate that the algorithm is especially effective in analysing epitaxially grown crystallites not only for various orientations, but also if different polymorphs are present in the film.

An overview of the Smithsonian Affiliations program and its reach in communities across the United States. Is the Smithsonian in your neighborhood?

The post Smithsonian Affiliates in your neighborhood appeared first on Smithsonian Insider.

Now that we have a deal between Time Warner Cable and CBS, we can turn our Hollywood focus back on the movie industry.

Steve Julian: Business analyst Mark Lacter, would you agree it's been an up and down summer at the box office?

Mark Lacter: It's been a flaky summer for Hollywood, Steve.  On the plus side, ticket revenue was up more than 10 percent, and attendance increased around six-and-a-half percent compared with last year (this covers the first week of May through Labor Day weekend).  The problem is that the studios and their investors spent huge amounts of money to make a lot of these movies, and they had to compete in a very crowded market - 23 big-budget films came out this summer, which is way higher than normal, and some of them never had a chance.

Julian: Some examples?

Lacter: Probably the biggest clunker was "The Lone Ranger," which could end up losing close to $200 million for Disney.  Another big disappointment was "White House Down," which was distributed by Sony and brought in only $140 million, which for a big-budget action film is really bad.  Even a film like "Pacific Rim," which did well at the box office, might still end up in the red because the production and marketing costs were so high.

Julian: And summer, of course, is the time when studios want to bring out these monster releases -

Lacter: - right, what they call "tent poles" - and in that category, the biggest winner was Disney's "Iron Man," which took in $1.2 billion.  Also having a great summer was "Monsters University" from Pixar, with $700 million.  You also had "Despicable Me 2" and "Fast and Furious 6," which might not be our cup of tea (speak for yourself, it takes me back to my police car days!), but did very well for Universal.  Eight of the top 12 films this summer were sequels - and yet, sequels were no guarantee of success (a number of them really struggled).  And, some non-blockbuster films found considerable success: "Now You See Me" from Lionsgate only cost $75 million to make.

Julian: So, in some ways, Hollywood was its usual unpredictable self.

Lacter: That's right - and don't expect any big changes in strategy when it comes to big-budget films.  The prospect of having huge success with one of these blockbusters is just too great, but perhaps more important is the fact that many of these films are financed by multiple groups of investors, and so the risk is spread around.  It's not like the old days when a studio bankrolled the whole thing.

Julian: Though, sounds like it's bad news for the city of Los Angeles: the "Man of Steel" sequel is going to be shot in Michigan?

Lacter: Mayor Garcetti has actually declared a state of emergency because the city keeps losing business to other states that offer big tax incentives to films - what's known as runaway production.  The truth is that business has been lost over the years, but L.A. is hardly in any danger of losing its spot as the center of entertainment.  And, you can see that with the L.A. County Board of Supervisors signing off on Disney's plan for a TV and movie production facility near Santa Clarita that will add more than a half-million square feet of studio space.

Julian: And, Universal's expanding, too.

Lacter: Earlier this year, Universal was given the approval to build more production facilities, and Paramount is planning an expansion, as well.  Now, these are all very ambitious projects - not the sort of investments that would be made if these studios were looking elsewhere to make movies and TV shows.  And, of course, they mean jobs - actually, employment levels in the entertainment industry have remained fairly steady going back the last decade.

Julian: Are there states that are pulling back their incentives?

Lacter: Yes, the state of North Carolina, which has been especially aggressive in using tax incentives to draw in movies and television going back to the 80s, is phasing out the giveaways because legislators have decided that the economic benefits aren't worth the tax revenues being lost.  And, other states with tax incentive programs are pulling back as well - they're finding that the payback is very difficult to measure.

Mark Lacter writes for Los Angeles Magazine and pens the business blog at LA Observed.com.

This content is from Southern California Public Radio. View the original story at SCPR.org.

Long ago, one great ocean flowed between North and South America. When the narrow Isthmus of Panama joined the continents about 3 million years ago, […]

The post Recent Connection Between North and South America Reaffirmed appeared first on Smithsonian Insider.

The crystal structure of vanthoffite, Na6Mg(SO4)4, was redetermined and refined with anisotropic displacement parameters for all atoms. Here, for the first time, we give its detailed description.

The crystal structure of vanthoffite {hexasodium magnesium tetrakis[sulfate(VI)]}, Na6Mg(SO4)4, was solved in the year 1964 on a synthetic sample [Fischer & Hellner (1964). Acta Cryst. 17, 1613]. Here we report a redetermination of its crystal structure on a mineral sample with improved precision. It was refined in the space group P21/c from a crystal originating from Surtsey, Iceland. The unique Mg (site symmetry overline{1}) and the two S atoms are in usual, only slightly distorted octahedral and tetrahedral coordinations, respectively. The three independent Na atoms are in a distorted octahedral coordination (1×) and distorted 7-coordinations intermediate between a `split octahedron' and a pentagonal bipyramid (2×). [MgO6] coordination polyhedra interchange with one half of the sulfate tetrahedra in <011> chains forming a (100) meshed layer, with dimers formed by edge-sharing [NaO7] polyhedra filling the interchain spaces. The other [NaO7] polyhedra are organized in a parallel layer formed by [010] and [001] chains united through edge sharing and bonds to the remaining half of sulfate groups and to [NaO6] octahedra. The two types of layers interconnect through tight bonding, which explains the lack of morphological characteristics typical of layered structures.

A method is described for indexing grazing-incidence X-ray diffraction data of epitaxially grown thin films comprising various crystal orientations and/or polymorphs by measuring reciprocal-lattice vectors.

Elisa Reimer
Apr 23, 2020; 134:jcs246421-jcs246421
Articles

Vaishnavi Ananthanarayanan
Apr 17, 2020; 133:jcs245928-jcs245928
Meeting Report

Research to date on the effect of capital punishment on homicide rates is not useful in determining whether the death penalty increases, decreases, or has no effect on these rates, says a new report from the National Research Council.

Efforts to convert civilian research reactors from weapon-grade highly enriched uranium (HEU) to low enriched uranium (LEU) fuels are taking significantly longer than anticipated, says a congressionally mandated report from the National Academies of Sciences, Engineering, and Medicine.

Good morning. Welcome to the release of the report Preparing for Future Products of Biotechnology, from the National Academies of Sciences, Engineering, and Medicine.

Cognitive training, blood pressure management for people with hypertension, and increased physical activity all show modest but inconclusive evidence that they can help prevent cognitive decline and dementia, but there is insufficient evidence to support a public health campaign encouraging their adoption, says a new report from the National Academies of Sciences, Engineering, and Medicine.

A number of strategies used by the police to proactively prevent crimes have proved to be successful at crime reduction, at least in the short term, and most strategies do not harm communities’ attitudes toward police, finds a new report by the National Academies of Sciences, Engineering, and Medicine.

The U.S. Census Bureau should develop a detailed concept and implementation plan for an Annual Business Survey System (ABSS) to replace the current suite of largely separate annual economic surveys, says a new report by the National Academies of Sciences, Engineering, and Medicine.

Recently, questions have been raised about climate science. The National Academies have addressed many of these questions in our independent, evidence-based reports. We are speaking out to support the cumulative scientific evidence for climate change and the scientists who continue to advance our understanding.

NASA should collaborate with the Federal Aviation Administration (FAA), industry, academia to research the full effects that increased unpiloted air vehicle traffic would have on society, including ramifications to sound, privacy, environmental matters, and cybersecurity, says a new report from the National Academies of Sciences, Engineering, and Medicine.

; Credit: John Rabe

Dear Off-Ramp fans,

What is a blog, after all? Words and images.

And what is a radio story on the web? Words, images, and sound.

Can't they live together in harmony? We say YES.

And with that in mind, we're killing the Off-Ramp blog page.  

But don't fear; we're not cutting back on content: everything that would have found a home here - Marc Haefele's art reviews, recommendations for fun events, etc. -- will now be on the regular web page of the Off-Ramp radio show. 

All the old blog entries will continue to stay on this page as an archive, like Catherine Deneuve's fading vampire lovers in The Hunger.

This content is from Southern California Public Radio. View the original story at SCPR.org.

The Asian Garden Mall in Westminster, where voters will make a choice about whether to recall city leaders.; Credit: Dorian Merina/KPCC

Voters in Westminster will decide this spring whether to recall its mayor and two city councilmembers. The Orange County Registrar of Voters has signed off on petitions for a recall election.  

This content is from Southern California Public Radio. View the original story at SCPR.org.

Increasing noise levels are a global environmental concern, and have been linked to important health issues, such as heart disease and cognitive development. New Danish research has now shown that it is also associated with an increased risk of diabetes.

Relatively simple, low-cost measures, such as insulating walls and installing solar collectors and efficient heaters for hot water can significantly reduce energy consumption in housing developments, according to a recent study from Portugal. Energy-efficient homes not only benefit the environment, but were found to potentially reduce residents’ energy bills by over half in an apartment block studied by the researchers.

In a recent experiment in Norway, electrical appliance stores increased their sales of energy-efficient tumble driers when consumers were made aware of their cheaper lifetime operating costs by shop staff and a new product labelling system.

Public participation in developing coastal management plans can have numerous benefits, such as augmenting expert information with local knowledge and building trust, a new study has confirmed; however, challenges remain, say the researchers. They use the experiences of 10 case studies to make a series of recommendations regarding how to improve the efficiency of the process.

Long ago, one great ocean flowed between North and South America. When the narrow Isthmus of Panama joined the continents about 3 million years ago, it also separated the Atlantic from the Pacific Ocean. If this took place millions of years earlier, as recently asserted by some, the implications for both land and sea life would be revolutionary. Aaron O'Dea, staff scientist at the Smithsonian Tropical Research Institute (STRI), and colleagues writing in Science Advances firmly set the date at 2.8 million years ago.

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Improving the efficiency of milk production could produce significant annual savings and potentially cut the environmental impacts of the industry by up to a third, according to new research from Northwest Spain.

Although soil quality is best assessed using a wide range of indicators, a smaller set may be more practical and still provide the necessary information needed to choose between land management systems. This is the conclusion of a new study in Brazil that evaluated three different indexes of soil quality based on three sets of indicators.

A recent pan-European study has reviewed the factors which influence how annoyed a person feels about road traffic and aircraft noise. Among its findings, residents in terraced housing or apartments were less annoyed by road traffic noise than residents in semi-detached or detached housing.

Increasing noise levels are a global environmental concern, and have been linked to important health issues, such as heart disease and cognitive development. New Danish research has now shown that it is also associated with an increased risk of diabetes.

Humanity is demanding ever greater economic productivity at a time when natural resources, the input that feeds this productivity, are dwindling. To reduce pressure on key assets, such as water, minerals, fuel and land, we must use less of them, and we need to increase the efficiency and productivity of resources that we do use, to achieve more output per input. Put simply, we must do more with less. This thematic issue reports on research which helps guide the way to a more resource efficient society.

Energy efficiency is at the centre of EU policy for achieving a fundamental transformation of Europe’s energy systems by 2030. This Thematic Issue reveals the complexity of the issue of energy efficiency, its links with resource efficiency and the wide range of factors influencing it, from technology to social practices.

A new World Health Organisation (WHO) study has estimated that the health impact of environmental noise in western Europe could be up to 1.6 million healthy life years lost annually through ill health, disability or early death.

New research in Spain has explored solutions to reducing traffic noise, and suggests that the best option is to combine global measures, such as speed restrictions, and local measures, such as noise screens.

A recent pan-European study has reviewed the factors which influence how annoyed a person feels about road traffic and aircraft noise. Among its findings, residents in terraced housing or apartments were less annoyed by road traffic noise than residents in semi-detached or detached housing.

Increasing noise levels are a global environmental concern, and have been linked to important health issues, such as heart disease and cognitive development. New Danish research has now shown that it is also associated with an increased risk of diabetes.

Digital noise maps developed under the EU’s Environmental Noise Directive (END) are a useful way of assessing traffic noise exposure for local residents, according to a recent Swedish study. The END maps could also be used to standardise noise exposure information in noise and health research.

New EU-funded research from Finland has indicated that poor communication between producers of energy-efficient housing technologies and potential house-buyers is a major barrier to sustainable housing. It suggests that government bodies can encourage energy-efficiency innovations not only through regulations but also by improving information flow.

A recent study in Finland suggests new building regulations will lead to a substantial reduction in the total energy demand and CO2 emissions in new apartment buildings. This could have an impact on the source of energy used for new buildings. In particular, the demand for space heating will fall, which could have an impact on the source of energy used for new buildings.

Recent research from China suggests that partially converting both natural gas and coal into 'syngas' fuel for power generation can generate around 6 per cent more electricity than competing 'conventional' gasification methods. The savings arise from more efficient use of the chemical energy in gas and coal which is lost in a conventional burner.

Some scientists have suggested that, when all potential inefficiencies are accounted for, local food distribution systems may be less sustainable than globalised systems. However, new research examining the behaviour of participants in local food networks in France suggests that they can be as energy efficient as globalised systems.

In a recent experiment in Norway, electrical appliance stores increased their sales of energy-efficient tumble driers when consumers were made aware of their cheaper lifetime operating costs by shop staff and a new product labelling system.

Increasing energy efficiency in Europe is vital to achieving a sustainable economy and tackling climate change. However, new research has shown that lack of capital and concerns about costs of disruption are major barriers to implementing energy efficiency measures in the foundry industry.

Relatively simple, low-cost measures, such as insulating walls and installing solar collectors and efficient heaters for hot water can significantly reduce energy consumption in housing developments, according to a recent study from Portugal. Energy-efficient homes not only benefit the environment, but were found to potentially reduce residents’ energy bills by over half in an apartment block studied by the researchers.

Motivating homeowners to carry out energy-efficient refurbishments remains a significant challenge for policymakers. New research from Germany has called for more government incentives and better communication strategies to ensure homeowners are aware of the advantages of making energy saving changes to their homes.

US researchers have found that promoting the environmental benefits of energy-efficient products may actually deter some people from buying them. Since environmental issues have become politically polarised, those who hold more conservative views are less likely to purchase products that are marketed using environmental benefits, they argue.

Although low-income households consume less energy than wealthier households, they are still keen to learn how to save energy, for both economic and environmental reasons. This is the conclusion of a recent Swedish study which explored the energy-related behaviour of residents on low incomes. It provides insights which could help inform energy-awareness campaigns targeted at this section of the population.