Weare delighted to have partnered with the International Renewable Energy Agency and the Terrawatt Initiative in the development of the Open Solar Contracts which were launched recently at the World Future Energy Summit in Abu Dhabi. The publication...

The Low Carbon Contracts Company (“LCCC”), the counterparty for the UK’s Contracts for Difference (“CFD”) scheme, issued an advice note on 7 April 2020 confirming that COVID-19 is capable of constituting a Force Majeure...

MOSCOW (AP) " Russian President Vladimir Putin marked Victory Day, the...

Stars and Stripes is making stories on the coronavirus pandemic available free of charge. See other free reports here. Sign up for our daily coronavirus newsletter here. Please support our journalism with a subscription. MOSCOW — Russian President Vladimir Putin marked Victory Day, the anniversary of the defeat of Nazi Germany in World War II, in a ceremony shorn of its usual military parade and pomp by the coronavirus pandemic. In neighboring Belarus, however, the ceremonies went ahead in full, with tens of thousands of people in the sort of proximity that has been almost unseen in the world for months....

The Konya Tropical Butterfly Garden is the only place in Turkey where one can observe every phase of metamorphosis, from small larvae to colorful butterflies.

One of the issues which accommodation teams in the Education Sector will be grappling with will be students trying to terminate their accommodation contracts early because of the Coronavirus outbreak. The impact this will have on the institution&rsq...

Introduction Last Monday’s announcement from the Government placing the country in lockdown will be felt heavily by institutions who are trying to ensure continuity of provision to students and maintain quality and standards of teaching. In ou...

The coronavirus (COVID-19) pandemic has created an unprecedented human and economic crisis. Governments are taking strong actions, enforcing quarantines to reduce contagion, testing populations, building emergency intensive care units. Governments have also launched large fiscal stimulus plans to protect jobs and the economy, as well as temporary social protection programs such as income/food support, subsidies […]

The post Citizen Action is Central to the Global Response to COVID-19 appeared first on Inter Press Service.

Monetary easing measures adopted by the Bank of Japan lack boldness.

The country loses a veteran of diplomatic affairs and adviser to several prime ministers with the coronavirus death of former Foreign Ministry official Yukio Okamoto.

Turkey supports the embattled Tripoli government against rebel forces from the east led by Gen. Khalifa Hifter.

Prague Daily Monitor

After the weekend hack-a-thon proved that a CZK 401 million contract for a web based vignette system can be made in a few hours time, with 60 programmers, Prague's politicians are on a witch hunt. Next is the CZK 15 billion Prague metro maintenance contract which was signed without a public tender.

read more

Prague Daily Monitor

A 31 year-old Czech speaking man approached the entrance to the gate armed with two knives yesterday. When police became aware of the man being armed, the man threatened the police, demanding to be let into the building. Police apprehended the suspect, nobody was injured during the incident. The suspect faces up to four years in prison for violence against an official.

read more

Two-minute silence held for father-of-four Augustine Agyei-Mensah, a highly regarded learning disabilities nurse

• Coronavirus – latest updates
• See all our coronavirus coverage

Tributes have been paid to a learning disabilities nurse who died after testing positive for coronavirus.

Augustine Agyei-Mensah, known to his colleagues as Gus, was described as a “highly regarded team member” at Northamptonshire Healthcare NHS foundation trust (NHFT).

ISLAMABAD: The Universal Service Fund awarded contract worth PKR 588 million to Telenor Pakistan for providing hi-speed broadband in Sanghar Lot .The Federal Minister IT& Telecom, Syed Amin ul...

[[ This is a content summary only. Visit my website for full links, other content, and more! ]]

ISLAMABAD: Keeping in view the impact of COVID-19 pandemic, Pakistan’s GDP is going to contract by 1.6 percent in the ongoing fiscal 2019-20 and will grow to 2.9 percent in the next financial year 2020-21 and the loans from IMF and other multilateral and bilateral donors will help to ease...

ISLAMABAD: Keeping in view the impact of COVID-19 pandemic, Pakistan’s GDP is going to contract by 1.6 percent in the ongoing fiscal 2019-20 and will grow to 2.9 percent in the next financial year 2020-21 and the loans from IMF and other multilateral and bilateral donors will help to ease...

ISLAMABAD: Keeping in view the impact of COVID-19 pandemic, Pakistan’s GDP is going to contract by 1.6 percent in the ongoing fiscal 2019-20 and will grow to 2.9 percent in the next financial year 2020-21 and the loans from IMF and other multilateral and bilateral donors will help to ease...

Central Asia is improving food safety measures to share with the world some of the more than 8000 plant species, as well as livestock, from the region.

Bold action is required by policymakers and central bankers to keep the region’s economies afloat and contain the pandemic.

A forward-looking, regional approach is needed for countries in Central Asia to respond to the pandemic and prepare for future public health threats.

ADB has a vacancy for the position of Procurement and Contracts Officer in the Office of Administrative Services. The deadline for submitting applications is on 22 May 2020.

ADB has a vacancy for the position of Procurement and Contracts Officer in the Office of Administrative Services. The deadline for submitting applications is on 22 May 2020.

This publication features projects promoting gender mainstreaming in the energy, transport, water supply and sanitation, and irrigation subsectors in Azerbaijan, Georgia, the Kyrgyz Republic, Pakistan, Tajikistan, and Uzbekistan.

This video defines the various types of Subcontractors and how they are assessed and utilized in the performance of ADB projects.

A glimpse into how scaling-up investment in Hasfarm Holdings is helping women in Central Highlands, Viet Nam improve their economic status and reach their full potential.

Trade facilitation, by reducing trade costs and raising the efficiency of moving goods across borders, is integral to international trade.

The global financial architecture should focus on providing short-term lending facilities to improve the efficiency of developing projects.

The United States Oil Fund LP, the largest oil-focused exchange-traded product (ETP) in the country, is moving to spread out its investments in oil futures in response to extreme market turbulence,...

NASA on Thursday selected space firms SpaceX, Blue Origin and Dynetics to build lunar landing systems that can carry astronauts to the moon by 2024, the White House's accelerated deadline under the space agency's moon-to-Mars campaign. Jillian Kitchener has more.

At a ceremony honoring nurses at the White House on Wednesday, U.S. President Donald Trump contradicted a New Orleans nurse who said the availability of personal protective equipment has been 'sporadic.'

U.S. President Donald Trump on Thursday said it's "one of those things" after he learned that a White House valet tested positive for the coronavirus, noting contact with that person was limited. Gavino Garay has more.

At a ceremony honoring nurses at the White House on Wednesday, U.S. President Donald Trump contradicted a New Orleans nurse who said the availability of personal protective equipment has been 'sporadic.'

Dr. Anthony Fauci, director of the National Institute for Allergy and Infectious Disease, says all of the Zika infections in the United States were contracted outside the country. Rough Cut (no reporter narration)

U.S. President Donald Trump on Thursday said it's "one of those things" after he learned that a White House valet tested positive for the coronavirus, noting contact with that person was limited. Gavino Garay has more.

Title: Magnetic Fields Concentrate Drug Delivery
Category: Health News
Created: 4/23/2010 6:10:00 PM
Last Editorial Review: 4/26/2010 12:00:00 AM

Title: Kcentra Approved to Stop Severe Bleeding in Heart Patients
Category: Health News
Created: 4/30/2013 12:35:00 PM
Last Editorial Review: 5/1/2013 12:00:00 AM

Title: Contraception Safety Program for Acne Drug Failing in Canada
Category: Health News
Created: 4/25/2016 12:00:00 AM
Last Editorial Review: 4/26/2016 12:00:00 AM

Title: Health Tip: Avoid Concentrated Caffeine
Category: Health News
Created: 4/30/2018 12:00:00 AM
Last Editorial Review: 4/30/2018 12:00:00 AM

PMC released a new and improved journal list on March 31, 2009, after a comprehensive redesign for greater clarity and organization.

The new design not only combines the full-list and tabbed-list views of the previous version but also includes some new or updated features to provide users with a greater overview of the total PMC archive. Looking at this new list, you will find:

1. An Expanded View of PMC journals that includes NIH Portfolio
2. Special Collections
3. A New “Participation Level” Journal Category
4. Hide/Show Display Option for Predecessor Titles
5. Article and Journal Search Feature

For more information, see the complete article in the May-June issue of the NLM Technical Bulletin: https://www.nlm.nih.gov/pubs/techbull/mj09/mj09_pmc_redesign.html

Title: Birth Control Pills (List of Oral Contraceptives and Side Effects)
Category: Medications
Created: 12/31/1997 12:00:00 AM
Last Editorial Review: 1/30/2020 12:00:00 AM

Membrane-bound proteins have been proposed to mediate the transport of long-chain FA (LCFA) transport through the plasma membrane (PM). These proposals are based largely on reports that PM transport of LCFAs can be blocked by a number of enzymes and purported inhibitors of LCFA transport. Here, using the ratiometric pH indicator (2',7'-bis-(2-carboxyethyl)-5-(and-6-)-carboxyfluorescein and acrylodated intestinal FA-binding protein-based dual fluorescence assays, we investigated the effects of nine inhibitors of the putative FA transporter protein CD36 on the binding and transmembrane movement of LCFAs. We particularly focused on sulfosuccinimidyl oleate (SSO), reported to be a competitive inhibitor of CD36-mediated LCFA transport. Using these assays in adipocytes and inhibitor-treated protein-free lipid vesicles, we demonstrate that rapid LCFA transport across model and biological membranes remains unchanged in the presence of these purported inhibitors. We have previously shown in live cells that CD36 does not accelerate the transport of unesterified LCFAs across the PM. Our present experiments indicated disruption of LCFA metabolism inside the cell within minutes upon treatment with many of the "inhibitors" previously assumed to inhibit LCFA transport across the PM. Furthermore, using confocal microscopy and a specific anti-SSO antibody, we found that numerous intracellular and PM-bound proteins are SSO-modified in addition to CD36. Our results support the hypothesis that LCFAs diffuse rapidly across biological membranes and do not require an active protein transporter for their transmembrane movement.

Cellular membranes are not homogenous mixtures of proteins; rather, they are segregated into microdomains on the basis of preferential association between specific lipids and proteins. These microdomains, called lipid rafts, are well known for their role in receptor signaling on the plasma membrane (PM) and are essential to such cellular functions as signal transduction and spatial organization of the PM. A number of disease states, including atherosclerosis and other cardiovascular disorders, may be caused by dysfunctional maintenance of lipid rafts. Lipid rafts do not occur only in the PM but also have been found in intracellular membranes and extracellular vesicles (EVs). Here, we focus on discussing newly discovered functions of lipid rafts and microdomains in intracellular membranes, including lipid and protein trafficking from the ER, Golgi bodies, and endosomes to the PM, and we examine lipid raft involvement in the production and composition of EVs. Because lipid rafts are small and transient, visualization remains challenging. Future work with advanced techniques will continue to expand our knowledge about the roles of lipid rafts in cellular functioning.

ABSTRACT

The availability of energy has significant impact on cell physiology. However, the role of cellular metabolism in bacterial pathogenesis is not understood. We investigated the dynamics of central metabolism during virulence induction by surface sensing and quorum sensing in early-stage biofilms of the multidrug-resistant bacterium Pseudomonas aeruginosa. We established a metabolic profile for P. aeruginosa using fluorescence lifetime imaging microscopy (FLIM), which reports the activity of NADH in live cells. We identified a critical growth transition period during which virulence is activated. We performed FLIM measurements and direct measurements of NADH and NAD⁺ concentrations during this period. Here, planktonic (low-virulence) and surface-attached (virulence-activated) populations diverged into distinct metabolic states, with the surface-attached population exhibiting FLIM lifetimes that were associated with lower levels of enzyme-bound NADH and decreasing total NAD(H) production. We inhibited virulence by perturbing central metabolism using citrate and pyruvate, which further decreased the enzyme-bound NADH fraction and total NAD(H) production and suggested the involvement of the glyoxylate pathway in virulence activation in surface-attached populations. In addition, we induced virulence at an earlier time using the electron transport chain oxidase inhibitor antimycin A. Our results demonstrate the use of FLIM to noninvasively measure NADH dynamics in biofilms and suggest a model in which a metabolic rearrangement accompanies the virulence activation period.

IMPORTANCE The rise of antibiotic resistance requires the development of new strategies to combat bacterial infection and pathogenesis. A major direction has been the development of drugs that broadly target virulence. However, few targets have been identified due to the species-specific nature of many virulence regulators. The lack of a virulence regulator that is conserved across species has presented a further challenge to the development of therapeutics. Here, we identify that NADH activity has an important role in the induction of virulence in the pathogen P. aeruginosa. This finding, coupled with the ubiquity of NADH in bacterial pathogens, opens up the possibility of targeting enzymes that process NADH as a potential broad antivirulence approach.