Telehealth and GP service Babylon has rolled out a "COVID-19 Care Assistant" with a raft of features designed to help subscribers cope with symptoms during the pandemic, from the safety of their phone screen

ATP plays important roles outside the cell, but the mechanism by which it is arrives in the extracellular environment is not clear. Dunn et al. now show that decreases in cellular cholesterol levels mediated by the ABCG1 transporter increase ATP release by volume-regulated anion channels under hypotonic conditions. Importantly, these results may imply that cells that handle cholesterol differently might experience differential extracellular ATP release during hypotonicity.

Purinergic signaling by extracellular ATP regulates a variety of cellular events and is implicated in both normal physiology and pathophysiology. Several molecules have been associated with the release of ATP and other small molecules, but their precise contributions have been difficult to assess because of their complexity and heterogeneity. Here, we report on the results of a gain-of-function screen for modulators of hypotonicity-induced ATP release using HEK-293 cells and murine cerebellar granule neurons, along with bioluminescence, calcium FLIPR, and short hairpin RNA–based gene-silencing assays. This screen utilized the most extensive genome-wide ORF collection to date, covering 90% of human, nonredundant, protein-encoding genes. We identified two ABCG1 (ABC subfamily G member 1) variants, which regulate cellular cholesterol, as modulators of hypotonicity-induced ATP release. We found that cholesterol levels control volume-regulated anion channel–dependent ATP release. These findings reveal novel mechanisms for the regulation of ATP release and volume-regulated anion channel activity and provide critical links among cellular status, cholesterol, and purinergic signaling.

The ribosome biogenesis factor Las1 is an essential endoribonuclease that is well-conserved across eukaryotes and a newly established member of the higher eukaryotes and prokaryotes nucleotide-binding (HEPN) domain-containing nuclease family. HEPN nucleases participate in diverse RNA cleavage pathways and share a short HEPN nuclease motif (RφXXXH) important for RNA cleavage. Most HEPN nucleases participate in stress-activated RNA cleavage pathways; Las1 plays a fundamental role in processing pre-rRNA. Underscoring the significance of Las1 function in the cell, mutations in the human LAS1L (LAS1-like) gene have been associated with neurological dysfunction. Two juxtaposed HEPN nuclease motifs create Las1's composite nuclease active site, but the roles of the individual HEPN motif residues are poorly defined. Here using a combination of in vivo experiments in Saccharomyces cerevisiae and in vitro assays, we show that both HEPN nuclease motifs are required for Las1 nuclease activity and fidelity. Through in-depth sequence analysis and systematic mutagenesis, we determined the consensus HEPN motif in the Las1 subfamily and uncovered its canonical and specialized elements. Using reconstituted Las1 HEPN-HEPN' chimeras, we defined the molecular requirements for RNA cleavage. Intriguingly, both copies of the Las1 HEPN motif were important for nuclease function, revealing that both HEPN motifs participate in coordinating the RNA within the Las1 active site. We also established that conformational flexibility of the two HEPN domains is important for proper nuclease function. The results of our work reveal critical information about how dual HEPN domains come together to drive Las1-mediated RNA cleavage.

(Association for Computing Machinery) Today, the ACM Europe Technology Policy Committee (Europe TPC) of the world's largest society of computing professionals, the Association for Computing Machinery (ACM), has released detailed principles and practices for the development and deployment of 'contact tracing' technology intended to track and arrest the spread of COVID-19.

Known for her creative dance moves and twerking style, dancer-turned-artiste Daniiboo dropped a new twerking song, titled ' Drop It N Pop It', in an attempt to put a smile on her fans' faces in a time of unprecedented uncertainty. The song is...

We are pleased to announce a major new release with more than 150 new features and improvements!

We are pleased to announce a major new release with more than 150 new features and improvements!

We are pleased to announce a major new release with more than 150 new features and improvements!

We are pleased to announce a major new release with more than 100 new features and improvements!

We are pleased to announce a major new release with more than 150 new features and improvements!

We are pleased to announce a major new release with more than 125 new features and improvements!

Gather round as three men spend 30 minutes talking about tech. Wait, what do you mean 'no thanks'?! Matt Egan shares the mic with David Price and Henry Burrell to thrash out why Apple deemed a press release adequate to announce the successor to 2014's iPad Air 2. There's also a slick new red iPhone, and a black OnePlus 3T. What's with the limited editions, eh? Or are they even limited? The gang also chat about the developer preview of Android O, out now while Android N is barely months old. Is it too early to say if it'll kick ass, and why do Google and Apple need such long public lead time with new OS builds?  

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Jens Juul Holst
Dec 1, 2004; 53:S197-S204
Section V: The Incretin Pathway

Errol B. Marliss
Feb 1, 2002; 51:S271-S283
Section 6: Pusatile and Phasic Insulin Release in Normal and Diabetic Men

Freddie Freeman spent the offseason sharing excitement about the possibility that Josh Donaldson would not end up being the Braves' only significant offseason addition.

HARRISBURG, Pennsylvania (AP) — A Pennsylvania inmate whose dreadlocks violated a jail’s haircut policy has been released from solitary confinement after more than a year, although his federal lawsuit is still pending. A federal...

Former national footballer Arthur Lattimore passed away after a long battle with throat cancer. Lattimore, who represented Jamaica’s football team through­out the 1970s, died at his home in Florida, leaving behind five children, grandchildren, and...

The NFL revealed its 2020 regular-season schedule Thursday, with the Kansas City Chiefs kicking off their Super Bowl title defense against the Houston Texans in Week 1.

The New Orleans Saints released Pro Bowl offensive lineman Larry Warford after three seasons, the team announced Friday.

Conservation officials in Ontario are asking members of the public to be on the lookout for a former pet snake that was mistakenly released into the wild near a conservation area.

The Pentagon announced Tuesday that Sgt. Christopher Wesley Curry died Monday in Iraq in what officials describe as a non-combat-related incident.

The Department of Health and Human Services released Nov. 25 a five-minute video explaining how to search its list of excluded individuals and entities, called LEIE.

The handout covers strategies for helping prevent the transmission of suspected respiratory disease in the dental health care setting and answers frequently asked questions related to the virus, based on guidelines from the Centers for Disease Control and Prevention.

Washington — The Environmental Protection Agency announced March 13 that it was adding nearly 200 additional products that have qualified for use against SARS-CoV-2, the novel coronavirus that causes the disease COVID-19.

The U.S. Department of Labor Wage and Hour Division released new guidance March 26 to help workers and employers understand provisions included in the Families First Coronavirus Response Act.

The American Dental Association has released interim guidance for dentists on how to minimize the risk of COVID-19 transmission before, during and after treating dental emergencies.

The ADA, Organized Dentistry Coalition, and many state dental associations are asking the U.S. Department of Health and Human Services and Centers for Medicare & Medicaid Services to release funding from the Coronavirus Aid, Relief and Economic Security Act Provider Relief Fund to assist dental Medicaid providers.

The Department of Labor released an FAQ April 27 that addresses common questions about work search and job refusal when an individual receives Pandemic Unemployment Assistance.

The ADA Health Policy Institute held a webinar April 29 with leading experts from across the dental industry to discuss how COVID-19 has affected the dental economy and what they think the future will bring.

The Supreme Court on Friday granted a Justice Department request to temporarily block release of secret grand jury material from special counsel Robert Mueller's probe to Congress.

Ariana Grande and Justin Bieber released their music video for "Stuck With U."

Katy Perry announced "Daisies," a first single from her forthcoming fifth studio album and a rumored collaboration with Taylor Swift.

K-pop star Minzy shared a comeback schedule for her new solo single, "Lovely."

Gov. Kate Brown ordered the public release of annual school performance ratings last week after Oregon's biggest newspaper reported that a Brown appointee had delayed the release of the statistical rankings until after the high-stakes gubernatorial election Nov. 6.

2005.

Reaction and analysis from Fox News contributor Byron York and former Florida Attorney General Pam Bondi.

Sandra Manninen
Jun 21, 2017; 37:6125-6131
BehavioralSystemsCognitive

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