The insect eggshell is a multifunctional structure with several important roles, including generating an entry point for sperm via the micropyle before oviposition, serving as an oviposition substrate attachment surface, and functioning as a protective layer during embryo development. Eggshell proteins play major roles in eggshell tanning and hardening following oviposition and provide molecular cues that define dorsal–ventral axis formation. Precise eggshell formation during ovarian follicle maturation is critical for normal embryo development and the synthesis of a defective eggshell often gives rise to inviable embryos. Therefore, simple and accurate methods for identifying eggshell proteins will facilitate our understanding of the molecular pathways regulating eggshell formation and the mechanisms underlying normal embryo development. This protocol describes how to isolate and enrich eggshells from mature oocytes of Aedes aegypti mosquitoes and how to extract their eggshell proteins for liquid chromatography with tandem mass spectrometry (LC–MS/MS) proteomic analysis. Although this methodology was developed for studying mosquito eggshells, it may be applicable to eggs from a variety of insects. Mosquitoes are ideal model organisms for this study as their ovarian follicle development and eggshell formation are meticulously regulated by blood feeding and their follicles develop synchronously throughout oogenesis in a time-dependent manner.

After more than a century of searching, scientists have discovered a gene in snails that may control asymmetries inside many animals

When the National Transportation Safety Board office in Alaska first hears about a plane going down, protocol is to begin gathering information

On this episode of Generation Anthropocene, we dive into one of the most underappreciated elements on the periodic table

Food is a focal point for understanding broader environmental problems. In this podcast, we learn how food buyers are influenced in surprising ways.

An unlikely friendship with Guy Burgess, the infamous British double-agent, brought unexpected joy to Stanley Weiss

Paraglider Ben Lewis remembers spinning around wildly as he got pulled up higher and higher into the violent storm. That was when he realized, about 6,700 metres up in the sky and still rising, that he was not likely to see his family again.

In Fort Good Hope, N.W.T., work is underway on a modular home construction centre that local leaders say will help the community meet housing and employment goals.

An exhibition at the Huntington Library, Art Museum and Botanical Gardens explores how Western intellectuals viewed the climate crisis between 1780 and 1930

The bronze wreath immortalized the moment when the members of the Honor Guard removed their hats and placed them on the president's grave during his burial

As a child, the future president acquired a marine animal's skull, which became the first specimen in his natural history collection

During and after the Civil War, inventive illustrations allowed Democrats and Republicans to turn American ballots into powerful propaganda

When the U.S. Army massacred a Lakota village at Blue Water, dozens of plundered artifacts ended up in the Smithsonian. The unraveling of this long-buried atrocity is forging a path toward reconciliation

Texts like the "Sortes Astrampsychi" promised insights on clients' love lives, career prospects, financial woes and families

Before fitness influencers made getting your steps in a trend, pedestrianism had the nation on their feet

A Romare Bearden print served as a starting point for the American playwright's 1987 drama, which follows a Black family's struggle to decide the fate of an ancestral heirloom

Maker of the “world famous buttermilk drop,” New Orleans actor Dwight Henry is expanding his baking empire

There have been 25 confirmed measles cases in New Brunswick since October after the identification of 11 new cases, according to a news release Saturday from Public Health.

Families of Norton Elementary School won’t be seeing their kids return anytime soon. Repairs are going to take longer than expected and students are expected to be back in the school by the start of the next school year.

Interim PC Leader Glen Savoie urged members to find a common purpose rather than blame former leader Blaine Higgs.

Many of the buns sold at China Pastry are classics you would find in bakeries in Hong Kong and Chinatowns all over the world. It's taken decades of hard work, but owners Sally and Tony Wong say they've established a nice flow together over the years.

Saskatchewan's Serious Incident Response Team (SIRT) is investigating the role the Moose Jaw Police Service played in a crash that killed a 31-year-old man.

Since 1970, Lakehead Public Schools has operated Kingfisher Lake Outdoor Education Centre just outside of Thunder Bay, Ont. The CBC's Matt Fratpietro spent a morning with students from École Elsie MacGill Public School to learn more about the forests that surround the city.

Canadians hear the Last Post every year during Remembrance Day ceremonies. Warrant Officer Jeremy Maitland breaks down what it takes to play the melody perfectly.

Former Ohio state Senator Nina Turner discusses the 2024 elections.

P.E.I.'s capital budget has allocated $2.5 million to build up to five provincially owned cellphone towers, but so far no telecommunications companies have committed to using them.

The Town of Kensington, P.E.I., has committed to implementing a new campaign to end gender-based violence.

Lloyd Gates is one of the few surviving Second World War veterans on Prince Edward Island. He recounts the highs and lows of his wartime service in Holland and France.

With P.E.I's only full-time blood oncologist leaving at the end of November, Health P.E.I. was planning to transfer his patients to Dr. Philip Champion. Now Champion says he intends to retire in the spring.

Sault Ste. Marie has the highest rate of opioid deaths in Ontario so far this year, according to new numbers from the province's coroner's office.

A retired lawyer from Sault Ste. Marie has written a book that celebrates the working-class neighbourhood in which he grew up. The West End: A Magical Place Created by Giants is by Frank Sarlo, whose grandfather emigrated to Chicago from Italy, but ended up in Sault Ste. Marie with the promise of work on the railroad.

An 18-year-old man is scheduled to make a court appearance on Tuesday, after being charged by the OPP with attempted murder and aggravated assault on a 16-year-old female victim. The charges follow a violent incident in Cobalt, Ont., on Nov 3.

Max Topolnisky, a 100-year-old Second World War veteran, was out selling poppies for the Lockerby Legion in Sudbury, Ont., two days before he died, on Nov. 1, 2024.

Medical student Samuel Bonne had read everything he could to prepare for his group's dissection of a cadaver. But after the first cut, he left with lingering questions about what it means to lead a good life and die with dignity.

Over the weekend, RCMP says officers searched for answers in the death of the toddler, knocking door-to-door on Saturday in Ashern and the surrounding communities of Gypsumville as they canvassed for information that may shed light on the timeline surrounding the child's death.

StandardAero says new funding from the provincial and federal governments will help expand its Winnipeg operations by training more specialized technicians, managers, support personnel and engineers.

The Manitoba government made a "political compromise" in its decision to issue a reduced number of moose hunting licences to non-Indigenous hunters in northern parts of the province earlier this year, a lawyer representing a First Nation in the area has argued in court.

The city said in a news release on Thursday that the Mayor’s Task Force on Transparency, Access and Accountability — which is in charge of reviewing community concerns about openness at the city — is looking for your feedback.

Here’s how Hamilton and other cities in the area are marking Remembrance Day this year.

While people gather around the Gore Park Cenotaph in his hometown of Hamilton, Tyrell will be one of nine Canadians serving as a peacekeeper in the United Nations mission in South Sudan.

Bobby Smyrniotis, shut out in five previous nominations, finally has a Coach of the Year Award to go with his four Canadian Premier League titles.

Amsterdam banned demonstrations for three days from Friday while giving police emergency stop-and-search powers after overnight attacks on Israeli soccer supporters, as the Israeli government said it would fly many fans home.

Canada Soccer says an independent review has confirmed the Paris Olympics drone-spying scandal was not an isolated misstep.

LAFC opened the series with a narrow 2-1 victory in California and Vancouver replied with a decisive 3-0 win at home to set up a do-or-die matchup on Friday, but they failed to overcome L.A. and suffered a 1-0 defeat.

Bring Back the Maples is a volunteer effort to increase the forest canopy along Woolwich Townships' rural roads.

Waterloo region’s food landscape is more than farm stands and restaurants. There's a vast array of food producers, food makers and organizations that make up the food scene. And now, a new directory aims to make them easier to find. CBC KW's food columnist Jasmine Mangalaseril explored the directory.

Host Craig Norris plays a whole bunch of music in this week's show. Hear tunes from Danny Michel, Chastity, Fred Penner, Tragically Hip, Angela Saini, Laila and a lot more.

It took Kitchener resident Laura McBride and her family 80 years to discover what happened to her great uncle Thomas Ferguson Wilson after he died in April 1944 during a training mission.

Make The Season Kind is a month of giving in support of The Food Bank of Waterloo Region. Join CBC K-W for a kick-off show on Friday, Dec. 6 at TheMuseum in downtown Kitchener with performances by I, The Mountain, singer-songwriter Mandippal, local chef Terry Salmond and more.

Saskatchewan's police watchdog is investigating an incident that led to the suspension of five Saskatoon police officers and a sixth officer is also off work because of an unrelated incident.