A liquid crystal display panel, including: a pixel electrode formed on a first substrate; an alignment layer formed on the pixel electrode, wherein the alignment layer includes an alignment layer material and aligns first liquid crystal molecules in a direction substantially perpendicular to the pixel electrode; and a photo hardening layer formed on the alignment layer, wherein the photo hardening layer includes a photo hardening layer material and aligns second liquid crystal molecules to be tilted with respect to the pixel electrode, wherein the alignment layer material and the photo hardening layer material have different polarities from each other.

A liquid crystal display capable of realizing a high transmittance while maintaining favorable voltage response characteristics, and a method of manufacturing the same are provided. The liquid crystal display includes: a liquid crystal layer; a first substrate and a second substrate arranged to face each other with the liquid crystal layer in between; a plurality of pixel electrodes provided on a liquid crystal layer side of the first substrate; and an opposite electrode provided on the second substrate to face the plurality of pixel electrodes. One or both of a face on the liquid crystal layer side of the pixel electrode, and a face on the liquid crystal layer side of the opposite electrode includes a concavo-convex structure.

An optical element includes at least two stacked birefringent layers having respective local optical axes that are rotated by respective twist angles over respective thicknesses of the at least two layers, and are aligned along respective interfaces between the at least two layers. The respective twist angles and/or the respective thicknesses are different. The at least two stacked birefringent layers may be liquid crystal polymer optical retarder layers. Related devices and fabrication methods are also discussed.

According to one embodiment, a first gene encodes a reporter protein. The first gene is disposed at the downstream of the gene promoter. A second gene is disposed at the downstream of the gene promoter and encodes a replication origin-binding protein. An internal ribosome entry site is disposed between the first gene and the second gene. The transcription termination signal sequence encodes a signal for terminating the transcription of the first gene and the second gene. A replication origin sequence is recognized by the replication origin-binding protein.

A method of expanding and maintaining human embryonic stem cells (ESCs) in an undifferentiated state by culturing the ESCs in a suspension culture under culturing conditions devoid of substrate adherence is provided. Also provided are a method of deriving ESC lines in the suspension culture and methods of generating lineage-specific cells from ESCs which were expanded in the suspension culture of the present invention.

Thrombospondin 1 (TSP-1), TSP-2, interleukin 17B receptor (IL-17BR) and heparin-binding epidermal growth factor-like growth factor (HB-EGF) associated with stem cell activity and use thereof.

Methods of washing adherent cells, capable of effectively suppressing cell death due to proteolytic enzyme treatment for detaching the adherent cell from a culture vessel and subsequent cell treatment; cell-washing solutions used for the washing method; methods of producing cell suspensions for transplantation using the cell-washing solution; and kits comprising the cell-washing solution. Trehalose or its derivative or a salt thereof is added to physiological aqueous solutions to prepare cell-washing solutions containing trehalose or its derivative or a salt thereof as an active ingredient. The cell-washing solutions can be used to wash adherent cells before detaching the adherent cells from a culture vessel by proteolytic enzyme treatment to suppress cell death due to the proteolytic enzyme treatment. The concentration of trehalose applied to the cell-washing solution may be a concentration capable of suppressing the cell death due to the proteolytic enzyme treatment, such as 0.1 to 20 (w/v) %.

A multi-channel system and methods for sorting particles according to one or more characteristics of the particles. The system includes multiple flow cytometry units, each unit can have a nozzle for producing a fluid stream containing a desired population of particles in a mixture of particles. Each of the units may be operable to sort said desired population of particles by interrogating the fluid stream with a beam of electromagnetic radiation and classifying particles based on one or more characteristics of the particles. The system also includes a common fluid delivery system for delivering sheath fluid to each flow cytometer unit for producing respective fluid streams.

Methods and agents for reducing a level of an acetylated Tau polypeptide in a cell are provided. Methods for treating a tauopathy in an individual are also provided. Also provided is a method for diagnosing a cognitive impairment disorder in an individual. Methods for identifying an agent suitable for treating a tauopathy are also provided.

The invention is directed to a single-step method for rapidly and efficiently preparing protein-polymer conjugates, including an insulin-polymer conjugate. According to the method of the present invention, a protein and hydrophilic polymer are contacted in the presence of at least one organic solvent and at least one metal chelator, under conditions that promote the formation of a conjugate of the protein and polymer. Thus, the invention is directed to the site-specific modification of selected proteins, such as insulin, with poly(ethylene glycol) at residue PheB1. The invention also provides a pharmaceutical formulation for encapsulating the conjugate in a biodegradable polymer.

Devices and methods are provided for reducing matrix effects in protein precipitated bioanalytical samples comprising: a support, and a sorbent associated with the support capable of binding matrix interfering agents present in the bioanalytical sample, wherein the device further comprises filtering means for removing precipitated protein particles. The filtering means is a size exclusion filter or a polymeric or inorganic monolith having a maximum pore size less than or equal to the diameter of the particles to be removed from the sample, and can be integral with the sorbent or associated with the sorbent. The sorbent is characterized by sufficient selectivity between the matrix interfering agents and analytes of interest to provide retention of the matrix interfering agents while providing elution of the analytes of interest (e.g., a reversed phase or a polar modified reversed phase). Typical devices incorporating these features include luer syringe filters, individual filter cartridges, multiwell plates, pipette tips, or inline columns for multiple or single use.

The invention relates to methods and compositions for identifying and selecting maize plants with enhanced resistance to northern leaf blight. Maize plants generated by the methods of the invention are also a feature of the invention.

Isolated polynucleotides and polypeptides and recombinant DNA constructs particularly useful for altering agronomic characteristics of plants under nitrogen limiting conditions, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter functional in a plant, wherein said polynucleotide encodes an LNT1 polypeptide.

This invention provides recombinant DNA constructs and methods for manipulating expression of a target gene that is regulated by a small RNA, by interfering with the binding of the small RNA to its target gene. More specifically, this invention discloses recombinant DNA constructs encoding cleavage blockers, 5-modified cleavage blockers, and translational inhibitors useful for modulating expression of a target gene and methods for their use. Further disclosed are miRNA targets useful for designing recombinant DNA constructs including miRNA-unresponsive transgenes, miRNA decoys, cleavage blockers, 5-modified cleavage blockers, and translational inhibitors, as well as methods for their use, and transgenic eukaryotic cells and organisms containing such constructs.

The present invention comprises methods and systems for manipulation of media and particles, whether inert materials or biomaterials, such as cells in suspension cell culture. The methods and systems comprise use of an apparatus comprising a rotating chamber wherein the actions of the combined forces fluid flow force and centrifugal force form a fluidized bed within the rotating chamber.

The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.

An automated method for detecting the presence of a nucleic acid in a sample, where the method is performed within a housing of a self-contained, stand-alone analyzer. The method includes purifying the nucleic acid after it has been immobilized on a magnetically-responsive solid support. A pipette of the analyzer is used to form a reaction mixture comprising the purified nucleic acid and all reagents required to perform a nucleic acid amplification. Amplification products are synthesized that include a nucleotide sequence contained in the nucleic acid or the complement of the nucleic acid. The amplification products are exposed to a probe in a mixture, where the probe forms a hybrid with one of the amplification products. The formation of the hybrid in the mixture provides an indication of the presence of the nucleic acid in the sample.

In one aspect, there is provided a cell culturing substrate including: a cell culture surface having a film attached thereto, wherein the film includes one or more plasma polymerized monomers; and a coating on the film-coated surface, the coating deposited from a coating solution comprising one or more extracellular matrix proteins and an aqueous solvent, where the total extracellular matrix protein concentration in the coating solution is about 1 ng/mL to about 1 mg/mL.

The present invention relates, in general, to gene expression and, in particular, to a method of inhibiting the expression of a target gene and to constructs suitable for use in such a method.

[Problem to be Solved] The importance of sugar chains having α2,3- or α2,6-linked sialic acid at their non-reducing ends is known. Industrial production has been demanded for these sugar chain compounds. Particularly, the production of glycoprotein drugs or the like inevitably requires producing in quantity sugar chains having homogeneous structures by controlling the linking pattern (α2,6-linkage or α2,3-linkage) of sialic acid. Particularly, a triantennary or tetraantennary N-type complex sugar chain having sialic acid at each of all non-reducing ends is generally considered difficult to chemically synthesize. There has been no report disclosing that such a sugar chain was chemically synthesized. Furthermore, these sugar chains are also difficult to efficiently prepare enzymatically.[Solution]The present inventors have newly found the activity of sialyltransferase of degrading sialic acid on a reaction product in the presence of CMP and also found that formed CMP can be degraded enzymatically to thereby efficiently produce a sialic acid-containing sugar chain. The present inventors have further found that even a tetraantennary N-type sugar chain having four α2,6-linked sialic acid molecules, which has previously been difficult to synthesize, can be prepared at high yields by one-pot synthesis comprising the elongation reaction of a biantennary sugar chain used as a starting material without performing purification after each enzymatic reaction.

A seat cushion, in particular for an aircraft seat, the seat cushion comprising a seat part having a receiving surface adapted to receive a person and a reinforcing part supporting the seat part, wherein at least the reinforcing part contains expanded polypropylene (EPP), preferably comprising fire retardant properties. The invention further relates to a method for manufacturing such a seat cushion, a seat comprising such a seat cushion and a vehicle comprising such a seat.

A plurality of varactors are coupled via a first electrode to a shared terminal that in turn can operably couple to a source of control voltage. A second electrode for each varactor couples to a corresponding switch, where each switch couples to at least two different voltage levels. So configured, the second electrode of each varactor can be individually connected to either of two voltage levels. This can be leveraged to control, in coarse steps, the overall aggregate effective capacitance presented by these components. At least some of these varactors can have differing corresponding capacitances, the specific values of which can be selected in order to facilitate relatively equal spacing and substantially equal rates of reactance change versus the control voltage value between aggregate-capacitive reactance ranges as correspond to differing settings for the switches at various levels for the control voltage source.

An embodiment of the present invention provides an apparatus, comprising at least one anti-parallel pair VVC network comprised of two parallel VVCs with one biased in the opposite polarity of the other and at least one anti-series VVC network comprised of two VVCs configured in series, one biased in the opposite polarity of the other such that the resulting AC capacitive variations produce a desired capacitance variation.

A communication module is provided in which its characteristic of separation between its first and second tuner units is improved. The module is equipped with a circuit board having a first main surface, and a second main surface opposite to the first main surface; a first amplifier arranged on the first main surface, for amplifying a first signal; a first mixer arranged on the first main surface, for converting a signal supplied from the first amplifier to an intermediate-frequency signal; a second amplifier for amplifying a second signal; and a mixer for converting a signal supplied from the second amplifier to an intermediate-frequency signal, both arranged on the second main surface of the circuit board.

A system includes a filter and a tuner formed on an integrated circuit. The filter receives an input signal comprising a first number of channels and communicates an intermediate output signal comprising a second number of channels less than the first number of channels. The tuner is coupled to the filter and receives the intermediate output signal and communicates an output signal comprising a third number of channels less than the second number of channels.

A band-pass filter device includes: a plurality of band-pass filter elements on a principal plane of a substrate; wherein the band-pass filter elements correspond to a plurality of respective channels divided by frequency regions, and each have a plurality of piezoelectric resonators. Each of the piezoelectric resonators includes a piezoelectric film whose periphery is supported by the substrate, a first electrode formed on a lower surface of the piezoelectric film, a second electrode formed on an upper surface of the piezoelectric film and formed in a state of overlapping at least a part of the first electrode with the piezoelectric film interposed between the second electrode and the first electrode, a lower space formed between the substrate and the piezoelectric film, and an upper space formed over the piezoelectric film.

In an oscillating apparatus, a detection unit detects a frequency offset between an input signal and a reference signal. A code generation unit specifies a relationship among a code having a predetermined number of bits, the frequency offset, and a voltage to be applied to a voltage-controlled oscillator by a DAC, in accordance with a frequency offset detection state of the detection unit. The code generation unit also generates a frequency offset correction code having a predetermined number of bits in accordance with the specified relationship. The DAC applies the voltage to the voltage-controlled oscillator, in accordance with the relationship described above and the code generated by the code generation unit. The voltage controlled oscillator outputs an oscillator signal having an oscillation frequency corresponding to the voltage applied by the DAC.

An electrical component provides a ceramic element located on or in a dielectric substrate between and in contact with a pair of electrical conductors, wherein the ceramic element includes one or more metal oxides having fluctuations in metal-oxide compositional uniformity less than or equal to 1.5 mol % throughout the ceramic element. A method of fabricating an electrical component, provides or forming a ceramic element between and in contact with a pair of electrical conductors on a substrate including depositing a mixture of metalorganic precursors and causing simultaneous decomposition of the metal oxide precursors to form the ceramic element including one or more metal oxides.

A method and apparatus for use in a digitally tuning a capacitor in an integrated circuit device is described. A Digitally Tuned Capacitor DTC is described which facilitates digitally controlling capacitance applied between a first and second terminal. In some embodiments, the first terminal comprises an RF+ terminal and the second terminal comprises an RF− terminal. In accordance with some embodiments, the DTCs comprise a plurality of sub-circuits ordered in significance from least significant bit (LSB) to most significant bit (MSB) sub-circuits, wherein the plurality of significant bit sub-circuits are coupled together in parallel, and wherein each sub-circuit has a first node coupled to the first RF terminal, and a second node coupled to the second RF terminal. The DTCs further include an input means for receiving a digital control word, wherein the digital control word comprises bits that are similarly ordered in significance from an LSB to an MSB.

The invention provides an aqueous ink comprising a polymer having hydroxyl groups and a pigment, which can provide images excellent in scratch resistance and highlighter resistance and can inhibit the seeping out phenomenon of the polymer and the deterioration of storage stability and ejection characteristics even when it is stored for a long period of time. The aqueous ink comprises a polymer having hydroxyl groups and a pigment, wherein a proportion of a content of a polymer that is not adsorbed on the pigment out of the polymer having hydroxyl groups to the content of the pigment in the ink is 2.0 mass % or more, and a content (mass %) of the polymer that is not adsorbed on the pigment out of the polymer having hydroxyl groups is 0.20 mass % or less based on the total mass of the ink.

A pattern printing system and data processing method thereof are disclosed, which are suitable for printing patterns on printed circuit boards or data format rearrangement printing used in displays. The pattern printing method includes a process for interpreting scription data into matrix data, a procedure for modulating the print head resolution and the printing resolution, a procedure for interpreting and transmitting data commands, a procedure for rearranging memory data, and a procedure for firing data synchronously so as to achieve high-resolution printing and to continuously modulate any print data.

One of mark sensors is automatically selected in accordance with specifications of a thermal sheet. A cutting position on the thermal sheet is decided on the basis of an output of the selected one mark sensor.

A print shop consolidation system including a print shop consolidation management system with an application is provided. The application is used to (1) evaluate, with a set of information, an operational capacity of a first print shop to process both a first group of print jobs and a second group of print jobs, (2) evaluate, with the set of information, an operational capacity of the second print shop to process both the first and second groups of print jobs, and (3) use the evaluations of (1) and (2) to consolidate processing of the first and second groups of print jobs at one of first and second print shops.

An object of this invention is to provide a newspaper production system, having the structure of a system that is provided with a supply unit for a continuous web provided at an upstream side of a printing unit for printing on one surface and the other surface of a continuous web, and a processing unit for carrying out cutting and folding processing of a continuous web after printing at a downstream side, by making the area of an installation flat region for the printing unit for printing on one surface and the other surface of a continuous web, having an ink jet printing device group for printing one side of the continuous web and an ink jet printing device group for printing the other side of the paper sheet, small, and with which it is easy for a single person to carry out a newspaper production operation of monitoring the entire system. It is also an object of this invention to provide a newspaper production system and newspaper production method that enables production of a few sizes of a plurality of newspaper pages arranged horizontally as sheets constituting a newspaper by sequentially cutting, and being capable of producing a newspaper formed of various page configurations.

A method and to a device for dispensing data from a tachograph to an endless printing medium. A first division is plotted in the longitudinal direction of the printing medium, and a second division is plotted transversely with respect to the longitudinal direction of the printing medium. The first division represents a predetermined unit of time, in particular a day in generalized time coordinates, and the second division represents at least one variable recorded by the tachograph during the unit of time, the at least one variable is depicted by at least one graph.

A data processing apparatus is monitoring the first port of itself and the second port of itself. If the data processing apparatus receives data via the second port, it conducts a specific process to the data regardless of a data format of the data so as to convert the data into the data format that can be processed regardless of a data format of the data and sends converted data to the first port.

A method enables changes in the operation of a web printing system in response to web dimensional changes being detected. The method includes identifying a cross-process dimensional change in a web moving through a printing system, comparing the identified cross-process dimensional change to a predetermined threshold, and changing operation of a component in the web printing system in response to the cross-process dimensional change exceeding the predetermined threshold.

The present invention is a method which erase information printed on a recording medium if necessary and thus reuse the recording medium, and includes 1) decolorable ink compositions which are not erased during the use but erased by a dye readily decolorable by a decolorant, 2) a decolorant composition including a thermal initiator or photoinitiator, and 3) a method for coating the decolorant and applying heat or irradiating UV to erase the dye. According to the present invention, it is possible to prepare various writing inks, and particularly, when applied to recording medium with a magnetic stripe as well as the general purpose papers, it is possible to reduce the production cost significantly, obtain a large import substitution effect of the magnetic ink all of which are imported, and largely reduce the environmental pollution.

Currency bills are received, transported, and imaged to produce image data from which a visually readable image of each currency bill can be reproduced. Each of the currency bills includes a denomination, a serial number, and a set of secondary identifiers. One of the currency bills is determined to be a suspect bill. The suspect bill serial number is attempted to be extracted from the image data associated with the suspect bill. In response to failing to extract a complete serial number of the suspect bill, a serial number field in a suspect report is populated with a serial number snippet image.

An exemplary system for managing the deployment of a seismic data acquisition system uses a module configured to execute a plurality of task in the field by receiving one or more seismic devices. The module may include a power source that provides electrical power to the seismic devices. The module may also include a processor programmed to retrieve data stored in the seismic devices, perform diagnostics, facilitate inventory and logistics control, configure seismic devices and update data or pre-programmed instructions in the seismic device.

In one embodiment, a method of lighting is disclosed which includes apportioning a video display screen into a content portion and at least one light portion. The content portion displays video content and the light portion provides auxiliary lighting when energized to illuminate a subject adjacent the video display screen.

An apparatus and method that operates a flattener in an ink-based printing apparatus. The printing apparatus can include a media path configured to transport media sheets. The printing apparatus can include a marking module configured to jet ink drops to generate images on the media sheets. The printing apparatus can include a flattener configured to flatten the ink jet drops of the images on the media sheets in a flattener nip. The printing apparatus can include a release agent distributer configured to distribute release agent on a first rotational flattener member. The printing apparatus can include a controller configured to control the printing apparatus to reduce an inter-copy gap distance between a first media sheet and a second media sheet to prevent a first rotational flattener member from contacting a second rotational flattener member between the first media sheet and the second media sheet.

A method for producing printed articles that encompasses providing a first ink composition that contains metal oxide particles with an average particle size in the range of about 3 to about 300 nm; providing a second ink composition that contains colored pigments dispersed in an ink vehicle; providing a printable media having a bottom supporting substrate and an ink-absorbing layer with pore diameters that are smaller than the size of the metal oxide pigment particles and colored pigments; and sequentially applying, on the same print area, the first and the second ink compositions onto said printable media. Also disclosed herein are the printed articles obtained according to the present method.

This invention concerns a method and apparatus for dispensing minute quantities of highly viscous material from a jetting needle when an expelling mechanism is activated using a nozzle plate with a jetting chamber having a top formed by a diaphragm and a bottom in fluid communication with a jetting needle. Fluid enters the chamber from an inlet channel and is jetted from the chamber by deforming the diaphragm to block the channel and expel a drop of fluid from the needle. A diaphragm driven metering chamber and/or reservoir chamber can optionally be used provide fluid to the jetting chamber.

The inside of a housing of a printer device is cooled by the air from a ventilation port and, consequently, the temperature of solder SP on a mask surface supported by a mask holding frame inside the housing can be kept low. Moreover, provided is a mask cover for blocking the air flow from the ventilation port toward the mask, and the drying of the solder SP caused by the wind from the ventilation port is thereby inhibited as a result of the wind from the ventilation port not coming into contact with the solder SP on the mask surface. In this way, the solder SP is cooled while inhibiting the drying of the solder SP and, consequently, it is possible to appropriately inhibit the change in viscosity of the solder SP, and favorable printing can be realized.

A time recorder includes a first sensor that detects the side edge of a time card having a cut-out formed at at least one corner of the bottom, a second sensor that detects the bottom of the time card, and a card feeding unit that feeds the time card. When the time card is fed by this card feeding unit, a pulse counter of the card feeding unit counts the number of pulses of predetermined pulse signals after the first sensor detects the time card and until the second sensor detects the time card. Next, the front and back faces of the time card are determined based on the number of pulses that is a counting result. Hence, the front and back faces can be determined by the first sensor and the second sensor only.

After a reducing agent is added to a water reaction system containing silver ions to deposit silver particles by reduction, the silver particles are dried to obtain a silver powder which is heat-treated at a temperature of higher than 100° C. and lower than 400° C. The silver powder thus heat-treated has a maximum coefficient of thermal expansion of not greater than 1.5% at a temperature of 50° C. to 800° C., and has no heating peak when the silver powder is heated from 50° C. to 800° C. The silver powder has an ignition loss of not greater than 1.0% when the silver powder is ignited until the weight of the silver powder is constant at 800° C. The silver powder has a tap density of not less than 2 g/cm3 and a BET specific surface area of not greater than 5 m2/g.

A forging heat resistant steel of an embodiment contains in percent by mass C: 0.05-0.2, Si: 0.01-0.1, Mn: 0.01-0.15, Ni: 0.05-1, Cr: 8 or more and less than 10, Mo: 0.05-1, V: 0.05-0.3, Co: 1-5, W: 1-2.2, N: 0.01 or more and less than 0.015, Nb: 0.01-0.15, B: 0.003-0.03, and a remainder comprising Fe and unavoidable impurities.

New 6xxx aluminum alloy bodies and methods of producing the same are disclosed. The new 6xxx aluminum alloy bodies may be produced by preparing the aluminum alloy body for post-solutionizing cold work, cold working by at least 25%, and then thermally treating. The new 6xxx aluminum alloy bodies may realize improved strength and other properties.

Nanostructured Mn—Al, Mn—Al—C permanent magnets are disclosed. The magnets have high coercivities (about 4.8 kOe and 5.2 kOe) and high magnetization values. An intennetallic composition includes a ternary transition metal modified manganese aluminum alloy Mn—Al—Fe, Mn—Al—Ni, or Mn—Al—Co having at least about 80% of a magnetic τ phase and permanent magnetic properties. The alloy may have a saturation magnetization value of at least 96 emu/g with approximately 5% ternary transition metal replacing Al. The alloy may also have a saturation magnetization value of at least 105 emu/g with 10% ternary transition metal replacing Al.