"Frida: Beyond the Myth" aims to paint an intimate portrait of the artist through dozens of works created by Kahlo and photographs taken by her loved ones

The success is a giant leap toward the company's goal to take humans and cargo all the way to Mars on the world's biggest and most powerful launch vehicle

The sprawling estate, which is the largest privately owned home in the country, will open its doors in November after a month-long closure

The fragile 18th-century containers, which likely held alcoholic beverages that were shared among passengers and crew members, survived for centuries at the bottom of the Atlantic Ocean

A candidate for the Nova Scotia NDP has apologized and is no longer running for the party in the provincial election after her comments on Israel and the Holocaust drew concern from the Jewish organizations. 

Independent Elizabeth Smith-McCrossin is back on the campaign trail in Cumberland North, but opponents say the riding would be better represented by an MLA from an official party. 

As Nova Scotia politicians make their fall election promises, Julie Leggett is urging them to commit to helping people on income assistance stay cool during heat waves.

Creating transgenic mosquitoes allows for mechanistic studies of basic mosquito biology and the development of novel vector control strategies. CRISPR–Cas9 gene editing has revolutionized gene editing, including in mosquitoes. This protocol details part of the gene editing process of Aedes aegypti mosquitoes via CRISPR–Cas9, through testing and validating single-guide RNAs (sgRNAs). Gene editing activity varies depending on the sequence of sgRNAs used, so validation of sgRNA activity should be done before large-scale generation of mutants or transgenics. sgRNA is designed using online tools and synthesized in <1 h. Once mutants or transgenics are generated via embryo microinjection, sgRNA activity is validated by quick genotyping polymerase chain reaction (PCR) and DNA sequencing.

Mosquitoes take blood meals from a diverse range of host animals and their host associations vary by species. Characterizing these associations is an important element of the transmission dynamics of mosquito-vectored pathogens. To characterize mosquito host associations, various molecular techniques have been developed, which are collectively referred to as blood meal analysis. DNA barcoding has diverse biological applications and is well-suited to mosquito blood meal analysis. The standard DNA barcoding marker for animals is a 5' fragment of the cytochrome c oxidase I (COI) gene. A major advantage of this marker is its taxonomic coverage in DNA sequence reference databases, making it feasible to identify a wider range of mosquito host species than with any other gene. However, the COI gene contains high sequence variation at potential priming sites between vertebrate orders. Coupled with the need for primer sequences to be mismatched with mosquito priming sites so that annealing to mosquito DNA is inhibited, it can be difficult to design primers suitable for blood meal analysis applications. Several primers are available that perform well in mosquito blood meal analysis, annealing to priming sites for most vertebrate host taxa, but not to those of mosquitoes. Because priming site sequence variation among vertebrate taxa can cause amplification to fail, a hierarchical approach to DNA barcoding-based blood meal analysis can be applied. In such an approach, no single primer set is expected to be effective for 100% of potential host species. If amplification fails in the initial reaction, a subsequent reaction is attempted with primers that anneal to different priming sites, and so on, until amplification is successful.

Transposon-mediated transgenesis has revolutionized both basic and applied studies of mosquito vectors of disease. Currently, techniques such as enhancer traps and transposon tagging, which rely on remobilizable insertional mutagenesis, are only possible with transposon-based vector systems. Here, we provide general descriptions of methods and applications of transposon-based mosquito transgenesis. The exact procedures must be adapted to each mosquito species and comparisons of some differences among different mosquito species are outlined. A number of excellent publications showing detailed and specific protocols and methods are featured and referenced.

Newspaper editor Horace Greeley unsuccessfully ran against incumbent Ulysses S. Grant in November 1872. Twenty-four days later, he died of unknown causes at a private mental health facility

Buenos Aires, Argentina :: Miembros de la comunidad japonesa encuentran amigos en un barco de visita y escuchan el evangelio en su lengua materna.

La tripulación de técnicos del Logos Hope sabe que el mantenimiento del barco es una forma de hacer ministerio.

Headlines for November 01, 2024; Will Abortion Rights Decide 2024 Election? Amy Littlefield on Trump’s Misogyny & 10 Ballot Measures; Report from Wisconsin: John Nichols on Harris’s Madison Roots & Key Senate/House Races Nationwide; “Little Secret”? Elie Mystal on Trump’s Likely Plan to Steal Election with GOP House Speaker Johnson

Headlines for November 08, 2024; Democrats Deserted Working Poor: Bishop William Barber on Healthcare, Living Wages, Voting Rights; “Open Celebration of the Oligarchy”: Both Dems & GOP Sucked Up to Billionaires in 2024 Election; End the Arms: Humanitarian Chief Jan Egeland Urges U.S. to Stop Arming Israel Before Trump Takes Office

After Canada Post workers gave a 72-hour notice to strike early Tuesday, some Calgary business owners are worried about what this means for holiday season sales. 

The establishment of neural connectivity is a major part of neural development. The central nervous system (CNS) midline is the most characterized axon guidance choice point, and work in Drosophila has played a pivotal role in understanding the molecular mechanisms responsible. Axons respond to attractive cues such as Netrin via the Frazzled receptor, and repulsive cues such as Slit via Robo receptors. Both signals are expressed at the CNS midline, affect pioneer axons, and have dramatic effects on the axon scaffold as a whole. Here, we focus on previous research analyzing classic mutants in the Slit/Robo pathway, which can readily be detected with a dissecting microscope. We also discuss analyzing these mutants in a teaching lab situation. The combination of sophisticated genetics and reliable axonal markers in Drosophila allows phenotypic analysis to be performed at the single-cell level. The elaborate architecture of neurons is very sensitive to disruption by genetic mutations, allowing the effects of novel mutations to be easily detected and assessed.

CRISPR–Cas9 has revolutionized gene editing for traditional and nontraditional model organisms alike. This tool has opened the door to new mechanistic studies of basic mosquito biology as well as the development of novel vector control strategies based on CRISPR–Cas9, including gene drives that spread genetic elements in the population. Although the promise of the specificity, flexibility, and ease of deployment CRISPR is real, its implementation still requires empirical optimization for each new species of interest, as well as to each genomic target within a given species. Here, we provide an overview of designing and testing single-guide RNAs for the use of CRISPR-based gene editing tools.

SolidWorks' dominance in Mexican industry prompts purchase of 300 seats at leading academic institution

World's largest supplier of food weighing machines to automate custom product configuration with 3D CAD software

Extensive automation of time-consuming tasks gives designers fast, accurate tools for testing their ideas

3D Design-Inspired Gifts for Everyone on Your List

Early Black Friday deals are in effect on Apple's 2024 MacBook Pro M4, with every configuration — retail and CTO — eligible for a coupon discount.

You can score up to $150 off Apple's brand-new 2024 M4 iMac and get your hands on one of the best all-in-one desktops. Plus, AppleCare is discounted too.

Apple's 1TB M4 MacBook Pro 14-inch is eligible for a material early Black Friday discount at Amazon, along with savings on the 2024 Mac mini M4 and a record low price on the 13-inch MacBook Air 16GB.

Apple's late 2024 update to its Mac and MacBook ranges has brought M4 to the product line. Here's how to choose the best portable Mac for your budget for the 2024 holiday season.

Another year, and the South African love affair with Black Friday continues unabated, albeit not with the same frenzy that greeted it during its peak in 2019, say analysts.

Every Friday, OM Hong Kong meets Pakistani women and children in neighbourhood parks, in an effort to demonstrate the love of God.

OM teams in Costa Rica and El Salvador take Christmas to approximately 2,000 children during the run up to Christmas.

New sheets outlines how districts can support English-learner students, but concedes that "schools may not be able to provide all services in the same manner they are typically provided."

There are five key values to consider when separating campaign rhetoric from how a candidate would actually govern, writes Robert Feirsen.

Idaho officials asked a U.S. Supreme Court justice to block an injunction that allows a group backing an education ballot initiative to collect electronic signatures because of COVID-19.

Oneida historian Heather Bruegl will discuss her Indigenous tribe's connection to the Continental Army's encampment at Valley Forge during a free community event at 7 p.m. on Nov. 12 in Penn State Great Valley's conference center. The event is part of Valley Forge Park Alliance's Speaker Series. 

In a case involving a transgender track athlete, the Trump administration says female transgender athletes are seeking "special treatment" to compete in girls' or women's sports.

The judge said the law likely discriminates on the basis of transgender status in violation of the equal-protection clause of the U.S. Constitution.

After halting football practices and games this summer, a growing number of high schools are returning to the gridiron, despite the sport’s high risk of infection.

"It has become clear over time that [the edTPA] caused unintended barriers and burdens for teachers entering the profession," Georgia's state superintendent said.

Wastewater contains nutrients that can overfeed algae, leading to harmful algal blooms and pollution issues in the ocean and other waterways. A new study by researchers at Penn State tracked how these nutrients migrate from disposal sites in the Florida Keys, and the results have already informed wastewater practices in the region.

Penn State Wilkes-Barre will host a popular community craft show coordinated by Arts at Hayfield on Nov. 3.

Three-time Grammy Award-winning vocalist Samara Joy will perform her third annual “A Joyful Holiday” featuring the McLendon Family in concert at 7:30 p.m. Tuesday, Dec. 3, in Eisenhower Auditorium.

OM Argentina comes alongside a local believer to bring the Gospel to the town of Alpa Corral, in the Sierras of Córdoba, on Good Friday.

El programa ofrece a los inquilinos la oportunidad de mejorar su calificación crediticia sin endeudarse más, simplemente pagando el alquiler.   Dover, Del., 9 de enero de 2022 – El Programa Piloto de Notificación sobre Alquileres de Inquilinos de Delaware es un nuevo programa financiado por la Ley del Plan de Rescate Estadounidense y en […]

Delaware Affordable Housing Developers Facing Cost Increases Encouraged To Apply   Dover, Del., February 1, 2023 – The Delaware State Housing Authority (DSHA) is extending the submission deadline for the Market Pressure Relief Fund (MPRF) to 3:00 p.m. on Friday, February 3, 2023. Application materials can be found here: http://destatehousing.com/Developers/Developers.php. MPRF was announced in July […]

Hundreds of members of Manipur's Meitei community came out on the streets in Delhi and other cities in a silent candlelight protest, seeking the safety of six people who have been missing since an encounter broke out between suspected Kuki militants.

The National Capital Region is set to get its second major airport in April next year and with the first trial flights scheduled a few days from now at the Noida International Airport, NDTV has got an exclusive behind-the-scenes look.

Hundreds of members of Manipur's Meitei community came out on the streets in Delhi and other cities in a silent candlelight protest, seeking the safety of six people who have been missing since an encounter broke out between suspected Kuki militants.

A high-tech marijuana operation, using imported seeds and full-spectrum grow lights, has been busted in Greater Noida and an English graduate from Meerut has been arrested.

On September 26, NASA’s Atmospheric Waves Experiment (AWE) captured atmospheric gravity waves caused by Hurricane Helene’s powerful landfall along Florida’s Gulf Coast. These waves, observed as concentric ripples extending from Florida, highlight NASA’s efforts to understand how terrestrial weather can impact space weather and disrupt communication systems. AWE’s observations offer a critical step in decoding the effects of severe storms on the upper atmosphere.

Temporary codification of expanded telemedicine options helps all Delawareans Today Governor John Carney signed, via livestream, HS 1 for HB 348, sponsored by Representative David Bentz, which temporarily codifies a number of consumer-friendly provisions developed during COVID-19 that can increase access to health care due to expanding telemedicine. Insurance Commissioner Trinidad Navarro issued the following […]