olli Three People Injured In Separate Collisions By bernews.com Published On :: Sat, 13 Jul 2024 01:46:35 +0000 A male motorcycle rider was injured in a collision in St. George’s, and in a separate incident two males were injured in a collision in Smiths. A police spokesperson said, “Around 11:57pm on Thursday, July 11th, 2024, police received a report of a single vehicle road traffic collision involving a motorcycle at Pepper Hall/Orange Hole Road, […] Full Article Accidents and fires All News #Collision
olli Police Update On Three Vehicle Collision By bernews.com Published On :: Tue, 16 Jul 2024 12:11:05 +0000 The police provided an update following a three vehicle collision in Warwick last week. A police spokesperson said, “A Government van driver involved in a collision with two cars on Middle Road, near the junction with Longford Road in Warwick around 3pm Tuesday, 9th July 2024, was arrested on suspicion of impaired driving and later […] Full Article Accidents and fires All #Collision
olli Single Vehicle Collision In Devonshire By bernews.com Published On :: Fri, 19 Jul 2024 14:30:48 +0000 [Updated] A truck crashed on Middle Road in Devonshire this morning. A police spokesperson said, “Police officers are currently at the scene of a reported single vehicle collision in Devonshire. Details remain limited at this time. However, it is understood that a truck overturned along Middle Road, Devonshire, in the vicinity of Saltus Grammar [Lower […] Full Article Accidents and fires All #BermudaPolice #Collision #Traffic
olli Motorcyclist Injured In Collision In Sandys By bernews.com Published On :: Sat, 20 Jul 2024 02:25:03 +0000 A man has been transported to hospital following a collision in Sandys this evening. A police spokesperson said, “Around 9:25 PM on Friday, 19 July 2024 police and other first responders were dispatched to a report of a serious single vehicle collision involving a motorcycle, on Somerset Road in the vicinity of Scaur Drive, Sandys […] Full Article Accidents and fires All News #Collision
olli Pedal Cyclist Injured In Collision In Pembroke By bernews.com Published On :: Sun, 21 Jul 2024 13:44:19 +0000 A male pedal cyclist was taken to hospital with “serious head injuries” following a collision involving a car on Pitt’s Bay Road in Pembroke this morning. A police spokesperson said, “Around 9:08 a.m. on Sunday, July 21st, 2024, police and other first responders were dispatched to a serious road traffic collision involving a motorcar and […] Full Article Accidents and fires All News #Collision
olli Motorcyclist Injured In Collision In Pembroke By bernews.com Published On :: Mon, 29 Jul 2024 10:24:52 +0000 A 30-year-old male motorcycle rider was injured in a collision in Pembroke early this morning. A police spokesperson said, “Shortly before 12:45 a.m., on Monday, July 29th, 2024, police and Emergency Medical Services personnel, were dispatched to a reported single vehicle road traffic collision, involving a motorbike, near the junction of West Park Lane and North […] Full Article Accidents and fires All News #Collision
olli Man Injured In Collision In Southampton By bernews.com Published On :: Sat, 03 Aug 2024 12:53:52 +0000 A car driver was injured after crashing into a wall in Southampton last night. A police spokesperson said, “Around 9pm on Friday, August 2 2024, police and other emergency services were dispatched to a report of a single vehicle collision on Middle Road near the junction with Lighthouse Road, Southampton. “Details are limited at this […] Full Article Accidents and fires All News #Collision
olli Man Injured In Collision In St. George’s By bernews.com Published On :: Sun, 25 Aug 2024 09:11:32 +0000 A 50-year-old man has been transported to hospital for treatment after a single vehicle collision involving a motorcycle in St. George’s. A police spokesperson said, “Shortly after 9:00pm on Saturday 24th, August 2024, police and other first responders were dispatched to a report of a serious single vehicle collision involving a motorcycle, on Southside Road, […] Full Article Accidents and fires All News #Collision
olli Police Renew Witness Appeal For Fatal Collision By bernews.com Published On :: Thu, 05 Sep 2024 23:50:48 +0000 The Bermuda Police Service [BPS] is “renewing the witness appeal for anyone that can assist with the ongoing investigation regarding a single vehicle motorcycle collision that claimed the life of 50-year-old Steve Perinchief, Bermuda’s fifth road fatality this year.” A police spokesperson said, “In particular, members of the public who can confirm Mr. Perinchief’s whereabouts […] Full Article Accidents and fires All News #Collision #FatalAccidents #RoadTrafficFatality
olli Person Injured In Two Vehicle Collision In Sandys By bernews.com Published On :: Tue, 10 Sep 2024 01:52:46 +0000 A collision involving motorcars occurred in Sandys today [Sept 9], with one driver sustaining injuries. A police spokesperson said, “Around 5:50 PM on Monday 9 September 2024, police and other first responders were dispatched to a report of a two vehicle collision, involving motorcars on Somerset Road Sandys in the vicinity of Lantana Drive. “Information […] Full Article Accidents and fires All #Collision
olli Motorcyclist Injured In Southampton Collision By bernews.com Published On :: Mon, 23 Sep 2024 15:41:31 +0000 [Updated] A motorcyclist was injured in a collision in Southampton this morning. A police spokesperson said, “Around 9am Monday, 23rd September 2024, police officers responded to a reported two vehicle collision at the junction of Middle Road and South Road in Southampton. “Early information indicates that a tractor trailer truck driven in the eastbound lane […] Full Article Accidents and fires All News #Collision
olli Rider Remains Hospitalised After Collision By bernews.com Published On :: Wed, 25 Sep 2024 21:10:25 +0000 The rider of a motorcycle involved in a collision with a tractor trailer truck on Monday [Sept 23] in Southampton remains hospitalised. A police spokesperson said, “The rider of a motorcycle involved in a collision with a tractor trailer truck around 9am Monday, 23rd September 2024, at the junction of Middle Road and South Road […] Full Article Accidents and fires All News #Collision
olli Rider Injured In Three Vehicle Collision By bernews.com Published On :: Thu, 26 Sep 2024 15:05:06 +0000 A collision in Paget yesterday involving a car and two motorcycles resulted in one of the motorcycle riders being injured. A police spokesperson said, “Shortly after 4:00 p.m. on Wednesday, September 25, 2024, police, Bermuda Fire and Rescue along with Emergency Medical Services, attended a serious road traffic collision involving a motor car and two motorcycles […] Full Article Accidents and fires All News #Collision
olli Two Vehicle Collision On Harbour Road By bernews.com Published On :: Sun, 29 Sep 2024 20:14:53 +0000 [Updated] A two vehicle collision involving a car and a motorcycle occurred this afternoon [Sept 29] on Harbour Road in Warwick. A police spokesperson said, “Around 2:20pm Sunday, 29th September 2024, police officers responded to a reported two vehicle collision on Harbour Road in Warwick. “Details remain limited at this time. “However, it is understood […] Full Article Accidents and fires All #Collision
olli Woman Injured In Single Vehicle Collision By bernews.com Published On :: Mon, 30 Sep 2024 12:49:15 +0000 [Updated] Police responded to a single vehicle collision on North Shore Road in Hamilton parish this morning. A police spokesperson said, “Around 8:55am Monday, 30th September 2024, police officers responded to a reported single vehicle collision on North Shore Road in Hamilton parish. “Details remain limited at this time. “However, it is understood that a woman […] Full Article Accidents and fires All #Collision
olli Between 4 And 6 Collisions Being Reported Daily By bernews.com Published On :: Tue, 01 Oct 2024 23:37:15 +0000 There are “between 4 and 6 RTCs being reported daily,” and the police are urging people to “slow down, always drive sober, do not drive when stressed or tired, put your phone down and please wear your seatbelt and fasten your helmet.” Acting Superintendent Robert Cardwell said, “By design, it was hoped that increased traffic […] Full Article Accidents and fires All News #Collision #Traffic
olli Woman Injured In Collision Near Darrell’s Wharf By bernews.com Published On :: Fri, 04 Oct 2024 16:15:31 +0000 A 45-year-old female motorcyclist was treated for non-life-threatening injuries after colliding with a car near Darrell’s Wharf in Warwick. A police spokesperson said, “At last check, a female rider hurt in a reported two vehicle collision near Darrell’s Wharf in Warwick around 2:20pm Sunday, 29th September 2024, apparently received treatment at King Edward VII Memorial […] Full Article Accidents and fires All #Collision
olli Motorcyclist Injured In Collision In Warwick By bernews.com Published On :: Mon, 07 Oct 2024 22:19:50 +0000 A 30-year-old male motorcycle rider was injured in a collision in Warwick this afternoon [Oct 7]. A police spokesperson said, “Around 1:25 pm on Monday, October 7, 2024, police and other first responders attended a serious single vehicle collision involving a motorcycle at the junction of Middle Road and Ten Pin Crescent, Warwick. “Upon arrival, […] Full Article Accidents and fires All #Collision
olli Police Witness Appeal: Two Vehicle Collisions By bernews.com Published On :: Wed, 09 Oct 2024 19:00:47 +0000 On October 8, 2024, police found two abandoned vehicles in separate road traffic collisions in Smiths and Sandy’s, with no injuries reported. A police spokesperson said, “Around 1:25 a.m. on Monday, October 8, 2024 Police received a report of a single vehicle road traffic collision [RTC] on Middle Road, near the junction with Verdmont Road, […] Full Article Accidents and fires All #BermudaPolice #Collision
olli Two Vehicle Collision In Southampton Parish By bernews.com Published On :: Wed, 16 Oct 2024 18:29:03 +0000 There has been a two vehicle road traffic collision involving a motorcar and a motorcycle in the area of Barnes Corner in Southampton Parish. A police spokesperson said, “There has been a two vehicle road traffic collision involving a motorcar and a motorcycle in the area of Barnes Corner, Southampton Parish. “Details are limited at […] Full Article Accidents and fires All #Collision
olli Collision Involving Bus & Pedestrian In Hamilton By bernews.com Published On :: Wed, 30 Oct 2024 14:37:27 +0000 [Updated] “There has been a serious road traffic collision involving a PTB bus and a pedestrian at the junction of Bermudiana Road and Front Street in the City of Hamilton,” the police said this morning [Oct 30]. A police spokesperson said, “Details are limited at this time, however, the scene is currently being processed and traffic […] Full Article Accidents and fires All News #Collision #Traffic
olli 57-Year-Old Man Injured In Motorcycle Collision By bernews.com Published On :: Mon, 04 Nov 2024 17:31:50 +0000 A road traffic collision between two motorcycles yesterday [Nov 3] left one man with injuries, while the second driver left the scene before police arrived. A police spokesperson said, “Shortly after 5:15pm on Sunday, November 3, 2024, police attended a road traffic collision involving two motorcycles near the junction of Parson’s Road and Crossland Lane, […] Full Article Accidents and fires All News #BermudaPolice #Collision
olli Motorcycle Collision In Hamilton Parish By bernews.com Published On :: Thu, 07 Nov 2024 23:50:42 +0000 [Updated] “There has been a serious road traffic collision involving two motorcycles on Blue Hole Hill, Hamilton Parish,” the police said this evening [Nov 7]. “The roadway is currently closed, with traffic being diverted through the Grotto Bay compound. Motorists heading into or out of the east end are encouraged to delay their departure, if possible […] Full Article Accidents and fires All News #Collision
olli SLC-2L-15: Cross-Pollination By strobist.blogspot.com Published On :: Sun, 28 Feb 2021 01:01:00 +0000 Lighting with flash can give you more than just the ability to control the quality of your light. With the inherent consistency of light from (manual) flash, you can layer in slices of time as well. But first, you’ll want to lock down two things: the ambient light portion of your exposure, and your camera’s physical position.Read more » Full Article
olli Board Game Review: Rolling Realms By www.thatswhatjennisaid.com Published On :: Mon, 17 Jan 2022 04:20:00 +0000 At every company, there’s some guy trying desperately to figure out a way to harness a current wave of consumer demand and somehow direct it right onto the doorstep of the company. “Even better…”, that guy explains to rest of management, “If we can deliver something on *that* demand that our customers will gobble up and that will drive their demand up for our *other* established products, we’ve gone above and beyond! A cross-promotional windfall!” Well, it looks like someone at Stonemaier put that guy in charge of roll and write game development and Rolling Realms was the result. It’s meta game of sorts that mostly serves as an advertisement for the rest of the Stonemaier product line, as each card in this roll and write game is named after a different Stonemaier game title. On the plus side, Rolling Realms is a pandemic friendly, easy to learn, and quick to play roll and write that plays as easily over zoom with 20 people as it plays in person with a few people around a table. Every card presents a different way to earn victory points as it’s filled in, and in a standard game, 9 cards from the supply of 11 varieties are chosen and used (3 cards per round x 3 rounds). Environment sparing bonus: the cards are all laminated and dry erase markers are provided, so you can play unlimited games without killing endless trees. The Tapestry card gives me a headache with its Tetris like spatial relations exercise, but otherwise I enjoyed all the cards and their creative use of point collection. Despite its positives, Rolling Realms does not make the cut for my recommendations list. I played many, many games of Rolling Realms to give it a fair shake, and here’s the thing – it’s a perfectly adequate roll and write game. But in this modern era of board gaming, there are a ton of roll and write games on the market or in development and adequate just doesn’t cut it. Add on the creepiness factor of the cross-promotional marketing gimmick (BTW, can anyone tell me why the card for Red Rising is named “The Society” instead of RR?) and yeah…just no. Keep the game if someone gives it to you I suppose (I’m probably keeping mine), but don’t go out and spend your own money on Rolling Realms when there are so many other better roll and write games out there you could buy instead. I’m talking Cartographers. I’m talking Hex Roller. I’m talking Qwixx and Quinto. I’m talking Railroad Ink. I’m talking Noch Mal. And for the ultimate challenge, I’m talking Fleet or Hadrian’s Wall. As a final note, I want to let you know that I’m very sad I finally met a Stonemaier game that didn’t bring me joy. I mean, it was inevitable that it would happen someday, but it’s still sad. I've been reviewing games from Stonemaier for a few years now. I got drawn in by Scythe initially (amazing area control game) and then, with each new game the company released, I crossed my fingers and hoped that it would be awesome. I really respect Jamey Stegmaier as a designer and a business owner and I'm rooting for his continued success. And so far, it's all worked out, because I've fallen in love with each Stonemaier game that's come my way, outside of Rolling Realms. For example, Tapestry and Between Two Castles are amazing, as are their expansions, and I urge you to give them a try. Also, I hope Jamey doesn’t listen to any more bad ideas originating from the guy with the marketing gimmick idea that was behind Rolling Realms, whether he was one of the voices in Jamey’s creative imagination or an actual employee at the company. ------------------------------------------------- Publisher: Stonemaier Games Players: 1 - many Actual Playing Time (vs the guideline on the box): about 20 minutes per game Game type: roll & write, dice rolling Rating: Rating scale: OUI: I would play this game again; this game is ok. I probably would not buy this game myself but I would play it with those who own it and if someone gave it to me I would keep it. OUI OUI: I would play this game again; this game is good. I would buy this game. OUI OUI OUI: I LOVE THIS GAME. I MUST HAVE THIS GAME. NON: I would not play this game again. I would return this game or give it away if it was given to me. Full Article board game reviews dice rolling games roll and write Stonemaier Games
olli Brisbane Broncos star Ezra Mam charged over head-on collision, allegedly failing second drug test - ABC News By news.google.com Published On :: Wed, 13 Nov 2024 04:32:57 GMT Brisbane Broncos star Ezra Mam charged over head-on collision, allegedly failing second drug test ABC NewsBrisbane news live: Broncos’ Ezra Mam charged over drug test result after crash | Stab victim’s widower wants knife checks in schools | Dog rescued near abandoned boat Brisbane TimesBroncos player charged after car crash: 9News Latest Stories Season 2024, Short Video 9NowEzra Mam issued court notice after allegedly failing secondary drug test Fox SportsClub Statement: Ezra Mam Broncos Full Article
olli Internet Had a Dangerous Amount of Fun Trolling Pic of Trump, Melania And Ivanka With The Pope By cheezburger.com Published On :: Thu, 29 Aug 2019 19:00:00 -0700 Just when we thought we'd never get anything better than Donald Trump grasping that orb, we get this dark-humored, delightfully awkward pic that just oozes cringe. Naturally, people were ready to flood Twitter with some entertaining captions. Full Article twitter trolling donald trump funny politics pope
olli Trump's 'Exploding Trees' Comment Has Memers Trolling Like Crazy By cheezburger.com Published On :: Fri, 18 Sep 2020 07:00:00 -0700 As wildfires continue to ravage the West Coast, President Trump has been quick to dismiss climate change as one of the root causes, stating that countries in Europe like Austria don't suffer from wildfires because Europeans live in "forest cities" that are "managed better" yet are "more explosive" than the trees in California.We're not entirely sure what any of that means, and apparently neither do memers, so please enjoy the following memes about "forest cities" in Austria. Full Article donald trump funny memes dank memes shitposts twitter twitter memes politics
olli Donald Trump's Trying to Hang with the Times, by Trolling the Sh*t out of Hillary Clinton in Pokémon GO Campaign Ad By cheezburger.com Published On :: Fri, 15 Jul 2016 11:00:00 -0700 What even is this planet that we live on anymore though? Full Article Pokémon pokemon go trolling donald trump win politics
olli Trolling Cop WIN By cheezburger.com Published On :: Fri, 07 Dec 2012 16:02:00 -0800 Full Article freeway cars troll cop Video g rated win
olli Kink Men: Carter Collins and Colt Spence – “Big Fat Man Meat” By www.queerclick.com Published On :: Wed, 13 Nov 2024 05:02:19 +0000 New on Kink Men: Colt Spence has had a terrible morning. It’s taking him longer than he anticipated to complete an endeavor that required him to report to work on a Sunday to try and wrap it up. Fortunately, the workshop is situated close to the bus station in the heart of town, and Colt... View Article The post Kink Men: Carter Collins and Colt Spence – “Big Fat Man Meat” appeared first on QueerClick. Full Article Porn Site Updates Carter Collins Colt Spence KinkMen
olli 5 Things with LPCollins By archiveofourown.org Published On :: Mon, 11 Nov 2024 16:52:56 +0000 Every month or so the OTW will be doing a Q&A with one of its volunteers about their experiences in the organization. The posts express each volunteer's personal views and do not necessarily reflect the views of the OTW or constitute OTW policy. Today's post is with LPCollins, who volunteers as a Dutch translator and beta reader for the Translation Committee. How does what you do as a volunteer fit into what the OTW does? I am part of the Translation committee, more specifically as a translator and beta reader for the Dutch team. (Hallo, iedereen!) I have also recently found my way into the Tag Wrangling committee, but that is all still very new and shiny, so I’ll be focussing on my role in Translation for this post. That role pretty much means that I help with the translation of the AO3 FAQs and tutorials, news posts, email templates and the occasional tag or user ticket that arrives in Dutch, a rare treat when speakers of your language are very accustomed to using English in online spaces. I am very proud of the role that our committee gets to play in diversifying the OTW and slowly but surely making it more accessible for users world-wide. Compared to the userbase speaking Chinese, Spanish or Russian, Dutch may seem like a very tiny, not-so-important piece of that puzzle, but doesn’t that only make it cooler that we can support such languages too? What is a typical week like for you as a volunteer? One of my absolute favorite things about the Translation committee is that you get to schedule the work according to your own needs. I am a chaos demon with fluctuating energy levels, so I’m not sure about having a “typical week” and that’s perfectly fine with Translation.Basically, at some point one of our lovely volunteer managers will send me an assignment, either a document that needs to be translated from scratch or a translation from one of my teammates that I need to beta read. Assignments always have a deadline too, shorter ones for urgent news posts and longer ones for longer documents. Depending on the length of the document, what I need to do with it, my mood and the alignment of the stars, I might do it right away, or spread it out over a week, or just do it on the day of the deadline. Then I let our staff know that I’m done and they send me a new task and so on. There have been weeks when I didn’t complete a single task and there have been days when I completed two, and it just works for me. What made you decide to volunteer? Look, I seem to be living in a world where all the paid jobs aren’t very interesting and all the interesting jobs aren’t very paid. (Will take recommendations for other worlds.) Point is, I am always drawn to ways to help organizations that I care about with a hands-on approach. Before I joined the OTW, I was a volunteer for IMAlive’s crisis support chat (big shout-out to Random Acts for providing my training), which I loved doing but unfortunately became too time-consuming when I needed to start working on my master’s thesis. Since I had been spending a lot of time on AO3 for years at that point, the OTW was an easy next target for when I did have some spare time again.I also have a degree in Dutch and English linguistics and literature, so translator was definitely one of the most appealing OTW positions from the get-go. I distinctly remember waiting for a chance to apply, checking every Translation recruitment post that popped up on AO3 to see if they needed someone for Team Dutch yet, as the committee always recruits for specific languages. As soon as Dutch was listed in one of the posts, I didn’t hesitate about applying, and I was lucky enough to be welcomed to the team. Already two years have passed since then and I haven’t regretted it for a single second. What has been your biggest challenge doing work for the OTW? Not gonna lie, I have witnessed some big internal discussions since joining the OTW, both about real-world events and about our own processes. I feel like those have been fought over enough, though, so I’ll share a challenge that’s more specific for Team Dutch.In case you don’t know, Dutch is an official language in three countries: the Netherlands, Belgium and Suriname. Unfortunately, Suriname isn’t currently represented in our team, but we do have both Belgian and Dutch folks, and despite our countries being right next to each other, there are actually some differences between Belgian Dutch and Dutch… Dutch. (Just ask how we end our emails.) They definitely aren’t big enough to separate the languages, but we do try to walk that middle ground in our translations and make sure that everything sounds good for everyone. So sometimes I will be beta reading a document and change something that just sounds way too Dutch to me and sometimes one of my teammates will point out that they would never use my suggested turn of phrase in the Netherlands. As a linguist, I find this very interesting, but it is also quite challenging to avoid colloquialisms when you’re not even always aware of them! What fannish things do you like to do? First and foremost, I’m a fanfic writer. Always have been (even before I knew that there was a word for it) and always will be. As a writer, I also have an atrocious habit and it goes something like this: start a very long fic, pour out new chapters religiously for months and months, stumble upon a different fandom, come up with a fic idea for said new fandom that “surely won’t take long and that I just need to get out of my system so that I can return to the existing WIP in peace” aaaaand repeat. Dear reader, if you happen to be following one of my stories, I am so, so sorry, I swear I will finish it one day.The love of my life is Supernatural and I will always come back to it, but I’m also very good at falling very hard for other fandoms on the side, ranging from The Boys to Danny Phantom to the latest Neil Gaiman-related show to Helluva Boss to anything that Richard Speight, Jr. worked on. One of my favorite things to do is hyperfixate on something new, then grab my friends by the shoulders and shake them until they agree to watch it too so that I can yell at them about it.Of course I read fanfics too, mostly in short bursts when I’m looking for a very specific type of fic. For my wallet’s sake, I limit the number of Supernatural conventions I can go to to one per year. I perpetually have a Discord tab open to stay in touch with fellow fans, will occasionally scour YouTube for fanvids and spend the rest of my fandom time on the best social media site ever, Tumblr. (Kidding, not kidding.)Now that our volunteer’s said five things about what they do, it’s your turn to ask one more thing! Feel free to ask about their work in the comments. Or if you'd like, you can check out earlier Five Things posts. The Organization for Transformative Works is the non-profit parent organization of multiple projects including Archive of Our Own, Fanlore, Open Doors, Transformative Works and Cultures, and OTW Legal Advocacy. We are a fan-run, entirely donor-supported organization staffed by volunteers. Find out more about us on our website. Full Article
olli Fewer Black men are enrolling in HBCUs. Here's why and what's being done By www.npr.org Published On :: Mon, 28 Oct 2024 05:00:00 -0400 The absolute number of Black men enrolled at Historically Black Colleges and Universities is the lowest it's been since 1976. Full Article
olli News24 | Two people arrested over murder of ‘Noem My Skollie’ actor, insurance fraud suspected By www.news24.com Published On :: Tuesday Nov 12 2024 21:31:55 Cape Town police have arrested two suspects in connection with the murder of Noem My Skollie actor David Manuel and his friend, Alfonso Fisher, in Gugulethu last month. Full Article
olli Harris Supporter Outraged By Postcard Trolling… Did They Go Too Far? By clashdaily.com Published On :: Mon, 04 Nov 2024 10:15:55 +0000 She likes Kamala's polices enough to put a lawn sign up on her property, but when one toll with a postcard personalizes those policies... she loses it. The post Harris Supporter Outraged By Postcard Trolling… Did They Go Too Far? appeared first on Clash Daily. Full Article Videos
olli No infinite spin for planar total collision By www.ams.org Published On :: Wed, 16 Oct 2024 14:24 EDT Richard Moeckel and Richard Montgomery J. Amer. Math. Soc. 38 (), 225-241. Abstract, references and article information Full Article
olli Undercurrents: Episode 58 - The Birth of a New America, and Remembering Rosemary Hollis By brightcove.hs.llnwd.net Published On :: Thu, 18 Jun 2020 00:00:00 +0100 Full Article
olli Remembering Rosemary Hollis (1952-2020) By www.chathamhouse.org Published On :: Fri, 12 Jun 2020 07:51:15 +0000 Remembering Rosemary Hollis (1952-2020) News Release sysadmin 12 June 2020 Professor Rosemary Hollis, a highly respected authority on the Middle East, died suddenly last week. Rosy is remembered with great respect and affection, as a colleague and a friend. Full Article
olli Benefits of Collisional Cross Section Assisted Precursor Selection (caps-PASEF) for Cross-linking Mass Spectrometry [Research] By www.mcponline.org Published On :: 2020-10-01T00:05:25-07:00 Ion mobility separates molecules in the gas-phase based on their physico-chemical properties, providing information about their size as collisional cross-sections. The timsTOF Pro combines trapped ion mobility with a quadrupole, collision cell and a TOF mass analyzer, to probe ions at high speeds with on-the-fly fragmentation. Here, we show that on this platform ion mobility is beneficial for cross-linking MS (XL-MS). Cross-linking reagents covalently link amino acids in proximity, resulting in peptide pairs after proteolytic digestion. These cross-linked peptides are typically present at low abundance in the background of normal peptides, which can partially be resolved by using enrichable cross-linking reagents. Even with a very efficient enrichable cross-linking reagent, like PhoX, the analysis of cross-linked peptides is still hampered by the co-enrichment of peptides connected to a partially hydrolyzed reagent – termed mono-linked peptides. For experiments aiming to uncover protein-protein interactions these are unwanted byproducts. Here, we demonstrate that gas-phase separation by ion mobility enables the separation of mono-linked peptides from cross-linked peptide pairs. A clear partition between these two classes is observed at a CCS of 500 Å2 and a monoisotopic mass of 2 kDa, which can be used for targeted precursor selection. A total of 50-70% of the mono-linked peptides are prevented from sequencing, allowing the analysis to focus on sequencing the relevant cross-linked peptide pairs. In applications to both simple proteins and protein mixtures and a complete highly complex lysate this approach provides a substantial increase in detected cross-linked peptides. Full Article
olli South Africa’s 2019 Election: Polling Data and Party Prospects By www.chathamhouse.org Published On :: Thu, 07 Mar 2019 10:35:01 +0000 South Africa’s 2019 Election: Polling Data and Party Prospects 13 March 2019 — 12:30PM TO 1:30PM Anonymous (not verified) 7 March 2019 Chatham House, London On 8 May 2019, South Africans will vote in their sixth national election. Incumbent President Cyril Ramaphosa is leading his ANC party campaign, which promises inclusive economic growth and social transformation, including through a sustainable land reform programme. However, public frustrations with the party’s record of service delivery and government corruption after 25 years in power could threaten the ANC’s electoral dominance especially in urban areas. At this meeting, Professor David Everatt, head of the Wits School of Governance and political pollster, will present polling data and discuss the prospects and strategies of the main parties and their leaders ahead of the May election. Attendance at this event is by invitation only. Full Article
olli 'California, Trump on Collision Course' By blogs.edweek.org Published On :: Tue, 15 Nov 2016 00:00:00 +0000 A read of California's news outlets makes clear that the state is not going to accept President-elect Donald Trump's policies without a fight, particularly those on immigration. Full Article California
olli Retinal Input to Macaque Superior Colliculus Derives from Branching Axons Projecting to the Lateral Geniculate Nucleus By www.jneurosci.org Published On :: 2024-10-30T09:30:22-07:00 The superior colliculus receives a direct projection from retinal ganglion cells. In primates, it remains unknown if the same ganglion cells also supply the lateral geniculate nucleus. To address this issue, a double-label experiment was performed in two male macaques. The animals fixated a target while injection sites were scouted in the superior colliculus by recording and stimulating with a tetrode. Once suitable sites were identified, cholera toxin subunit B-Alexa Fluor 488 was injected via an adjacent micropipette. In a subsequent acute experiment, cholera toxin subunit B-Alexa Fluor 555 was injected into the lateral geniculate nucleus at matching retinotopic locations. After a brief survival period, ganglion cells were examined in retinal flatmounts. The percentage of double-labeled cells varied locally, depending on the relative efficiency of retrograde transport by each tracer and the precision of retinotopic overlap of injection sites in each target nucleus. In counting boxes with extensive overlap, 76–98% of ganglion cells projecting to the superior colliculus were double labeled. Cells projecting to the superior colliculus constituted 4.0–6.7% of the labeled ganglion cell population. In one particularly large zone, there were 5,746 cells labeled only by CTB-AF555, 561cells double labeled by CTB-AF555 and CTB-AF488, but no cell labeled only by CTB-AF488. These data indicate that retinal input to the macaque superior colliculus arises from a collateral axonal branch supplied by ~5% of the ganglion cells that project to the lateral geniculate nucleus. Surprisingly, there exist no ganglion cells that project exclusively to the SC. Full Article
olli What Happens When Galaxies Collide? By www.smithsonianmag.com Published On :: Fri, 04 Oct 2024 00:00:00 -0000 In about four billion years from now, the Milky Way and Andromeda galaxies will crash together Visualization: NASA, ESA, and F. Summers, STScI Simulation credit: NASA, ESA, G. Besla, Columbia University, and R. van der Marel, STScI Full Article
olli The Pollinating Cricket By www.smithsonianmag.com Published On :: Mon, 21 Oct 2024 00:00:00 -0000 For the first time ever, researchers observed a cricket as a pollinator for a flower Full Article
olli Former Poet Laureate Billy Collins Reads "The Unfortunate Traveler" By www.smithsonianmag.com Published On :: Thu, 31 Oct 2024 00:00:00 -0000 Smithsonian magazine's poetry consultant recites his poem commissioned for a special photography issue Full Article
olli Visualization of Apoptotic Ovarian Follicles during Aedes aegypti Mosquito Egg Maturation by Fluorescent Imaging Studies By cshprotocols.cshlp.org Published On :: 2024-10-01T07:08:54-07:00 In insects, oocyte resorption (oosorption) or follicular atresia is one of the key physiological processes and evolutionary strategies used to optimize reproductive fitness. Mosquitoes are ideal model organisms for studying egg maturation in arthropods, as their follicle development is initiated only following the ingestion of a blood meal, followed by a carefully orchestrated series of hormonally regulated events leading to egg maturation. A cohort of approximately 100 follicles per mosquito ovary begin developing synchronously. However, a significant fraction of follicles ultimately undergo apoptosis and oosorption, especially when available resources from the blood meal are limited. Therefore, simple, rapid, and reliable techniques to accurately evaluate follicular atresia are necessary to understand mechanisms underlying follicle development in insects. This protocol describes how to detect apoptotic follicle cells within the Aedes aegypti mosquito ovaries using a commercially available fluorescent-labeled inhibitor of caspases (FLICA). Caspases are key players in animal apoptosis. In this assay, the FLICA reagent enters the intracellular compartment of follicles in dissected mosquito ovaries and covalently binds to active caspases. The bound reagent remains within the cell and its fluorescent signal can be observed by confocal microscopy. Although this method was specifically developed for visualizing apoptotic ovarian follicles during Ae. aegypti mosquito egg development, it should be applicable to other mosquito tissues that undergo caspase-mediated program cell death in a time-dependent manner. Full Article
olli Drivers urged to watch for wildlife after 5 collisions involving moose in 30-minute window: Sask. RCMP By www.cbc.ca Published On :: Sat, 09 Nov 2024 12:39:45 EST Drivers are being warned to use caution on Saskatchewan's highways after five separate collisions involving moose were reported to RCMP in a 30-minute window on Friday evening. Full Article News/Canada/Saskatoon
olli Veterans enrolling at chef school find a familiar mission in the kitchen By www.cbc.ca Published On :: Mon, 11 Nov 2024 04:00:00 EST A Veterans Affairs Canada education and training benefit is helping retired soldiers, sailors and airmen fit into a new uniform at Ottawa's Le Cordon Bleu. Full Article News/Canada/Ottawa
olli Make it 7 straight: Winnipeg Jets keep rolling undefeated, edging Seattle in OT By www.cbc.ca Published On :: Fri, 25 Oct 2024 08:35:30 EDT Nikolaj Ehlers scored the winning goal 1:26 into overtime, and the Winnipeg Jets remained unbeaten this season with a 4-3 win over the Seattle Kraken on Thursday night. Full Article News/Canada/Manitoba