After eight months of maintenance and improvements to prepare the vessel for future service, Logos Hope sets sail from Subic Bay, Philippines.

The world’s largest floating book fair welcomes its five millionth visitor as the tour of Asia comes to a conclusion.

034 - Wednesday 26 June 2013 at 11.30 at the Pavillon Cambon Capucines in Paris

Here Mudassar Ahmed Khan has explained with an example, how to implement simple user login form with Logout functionality in ASP.Net using C# and VB.Net. The login form has been implemented using ASP.Net Login control and Forms Authentication. The Logout functionality will be performed with the help of LoginStatus control in ASP.Net.

Here Mudassar Ahmed Khan has explained with an example, how to implement Logout functionality in ASP.Net MVC Razor. This User Login form will be implemented using Custom Forms Authentication and Entity Framework in ASP.Net MVC Razor. The Logout functionality will be performed using the FormsAuthentication.SignOut method in ASP.Net MVC Razor.

H K Digital Online offers Print Designing, Brochure designing, logo designing, leaflet designing, graphic designing with affordable rates.

THC-Hydra is a high quality parallelized login hacker for Samba, Smbnt, Cisco AAA, FTP, POP3, IMAP, Telnet, HTTP Auth, LDAP, NNTP, MySQL, VNC, ICQ, Socks5, PCNFS, Cisco and more. Includes SSL support, parallel scans, and is part of Nessus.

THC-Hydra is a high quality parallelized login hacker for Samba, Smbnt, Cisco AAA, FTP, POP3, IMAP, Telnet, HTTP Auth, LDAP, NNTP, MySQL, VNC, ICQ, Socks5, PCNFS, Cisco and more. Includes SSL support, parallel scans, and is part of Nessus.

Nutrient acquisition is a central challenge for all organisms. For the fungal pathogen Candida albicans, utilization of amino acids has been shown to be critical for survival, immune evasion, and escape, while the importance of catabolism of host-derived proteins and peptides in vivo is less well understood. Stp1 and Stp2 are paralogous transcription factors (TFs) regulated by the Ssy1-Ptr3-Ssy5 (SPS) amino acid sensing system and have been proposed to have distinct, if uncertain, roles in protein and amino acid utilization. We show here that Stp1 is required for proper utilization of peptides but has no effect on amino acid catabolism. In contrast, Stp2 is critical for utilization of both carbon sources. Commensurate with this observation, we found that Stp1 controls a very limited set of genes, while Stp2 has a much more extensive regulon that is partly dependent on the Ssy1 amino acid sensor (amino acid uptake and catabolism) and partly Ssy1 independent (genes associated with filamentous growth, including the regulators UME6 and SFL2). The ssy1/ and stp2/ mutants showed reduced fitness in a gastrointestinal (GI) colonization model, yet induced greater damage to epithelial cells and macrophages in a manner that was highly dependent on the growth status of the fungal cells. Surprisingly, the stp1/ mutant was better able to colonize the gut but the mutation had no effect on host cell damage. Thus, proper protein and amino acid utilization are both required for normal host interaction and are controlled by an interrelated network that includes Stp1 and Stp2.

ABSTRACT

In numerous instances, tracking the biological significance of a nucleic acid sequence can be augmented through the identification of environmental niches in which the sequence of interest is present. Many metagenomic data sets are now available, with deep sequencing of samples from diverse biological niches. While any individual metagenomic data set can be readily queried using web-based tools, meta-searches through all such data sets are less accessible. In this brief communication, we demonstrate such a meta-metagenomic approach, examining close matches to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in all high-throughput sequencing data sets in the NCBI Sequence Read Archive accessible with the "virome" keyword. In addition to the homology to bat coronaviruses observed in descriptions of the SARS-CoV-2 sequence (F. Wu, S. Zhao, B. Yu, Y. M. Chen, et al., Nature 579:265–269, 2020, https://doi.org/10.1038/s41586-020-2008-3; P. Zhou, X. L. Yang, X. G. Wang, B. Hu, et al., Nature 579:270–273, 2020, https://doi.org/10.1038/s41586-020-2012-7), we note a strong homology to numerous sequence reads in metavirome data sets generated from the lungs of deceased pangolins reported by Liu et al. (P. Liu, W. Chen, and J. P. Chen, Viruses 11:979, 2019, https://doi.org/10.3390/v11110979). While analysis of these reads indicates the presence of a similar viral sequence in pangolin lung, the similarity is not sufficient to either confirm or rule out a role for pangolins as an intermediate host in the recent emergence of SARS-CoV-2. In addition to the implications for SARS-CoV-2 emergence, this study illustrates the utility and limitations of meta-metagenomic search tools in effective and rapid characterization of potentially significant nucleic acid sequences.

IMPORTANCE Meta-metagenomic searches allow for high-speed, low-cost identification of potentially significant biological niches for sequences of interest.

It’s the weekend before E3. What can we do but get on the hype train? First stop is Square Enix. Second stop is XSEED. Third...

Design a logo for this year’s World Environment Day and win a trip to Milan, Italy.

This little boy looks like he might be blogging over spilled milk, in fact he's concerned about the future of his environment. The Global Warming Rug by Mexican design cooperative NEL, featuring Emiliano Godoy, has created quite a

Where did your paper coffee cup come from? Does it support a red-cockaded woodpecker? Or a gopher tortoise?

CEPSA's logo installation