On Thursday, the Delhi government had launched an official website for its an e-token system under which customers would be given a specific time for purchasing liquor in the wake of violations of social distancing norms by people crowding such outlets.{image-liquor-1588223729.jpg

A man is dead after a single-vehicle motorcycle crash in southeast Calgary.

The dextransucrase DSR-OK from the Gram-positive bacterium Oenococcus kitaharae DSM17330 produces a dextran of the highest molar mass reported to date (∼109 g/mol). In this study, we selected a recombinant form, DSR-OKΔ1, to identify molecular determinants involved in the sugar polymerization mechanism and that confer its ability to produce a very-high-molar-mass polymer. In domain V of DSR-OK, we identified seven putative sugar-binding pockets characteristic of glycoside hydrolase 70 (GH70) glucansucrases that are known to be involved in glucan binding. We investigated their role in polymer synthesis through several approaches, including monitoring of dextran synthesis, affinity assays, sugar binding pocket deletions, site-directed mutagenesis, and construction of chimeric enzymes. Substitution of only two stacking aromatic residues in two consecutive sugar-binding pockets (variant DSR-OKΔ1-Y1162A-F1228A) induced quasi-complete loss of very-high-molar-mass dextran synthesis, resulting in production of only 10–13 kg/mol polymers. Moreover, the double mutation completely switched the semiprocessive mode of DSR-OKΔ1 toward a distributive one, highlighting the strong influence of these pockets on enzyme processivity. Finally, the position of each pocket relative to the active site also appeared to be important for polymer elongation. We propose that sugar-binding pockets spatially closer to the catalytic domain play a major role in the control of processivity. A deep structural characterization, if possible with large-molar-mass sugar ligands, would allow confirming this hypothesis.

If history is any guide, adversity offers maximum value. The global financial crisis in 2008 culminated in the index being up nearly 100% in 2009.

The National Stock Exchange (NSE) on Wednesday said it was working to equip its systems to handle crises such as that caused by the crash in crude prices.

With crude price low, the govt should think of moving completely to DBT for food, fertiliser and power.

A truck driver has been freed from his vehicle and is being airlifted to hospital after a crash in Eastern Bay of Plenty.Emergency services were called to a report that a truck crashed at the intersection of State Highway 30 and...

A truck driver is in a critical condition after being trapped for more than four hours after his truck rolled near Rotorua this morning. Police were notified of a truck that rolled and was in a ditch at 9:33am on the intersection...

EMS said the man died at the scene.

Vanessa Bryant filed a claim against the Los Angeles County Sheriff's Department, contending deputies shared unauthorized photos of the site where her husband and daughter died in a helicopter crash in January.

Search teams bring in more bodies from the AirAsia crash to a military base in Indonesia. Rough Cut (no reporter narration).

Vanessa Bryant filed a claim against the Los Angeles County Sheriff's Department, contending deputies shared unauthorized photos of the site where her husband and daughter died in a helicopter crash in January.

In the second installment of The Voice's virtual happy hour, the show's coaches shared their attempts at keeping busy and sane during quarantine, by sipping cocktails and sharing their...

In the second installment of The Voice's virtual happy hour, the show's coaches shared their attempts at keeping busy and sane during quarantine, by sipping cocktails and sharing their...

In the second installment of The Voice's virtual happy hour, the show's coaches shared their attempts at keeping busy and sane during quarantine, by sipping cocktails and sharing their...

Mark Webber has apologised for crashing into Lewis Hamilton in the closing stages of the Australian Grand Prix

Fernando Alonso has posted a video on his YouTube account thanking fans for their support and promising them he will seen them "very soon on the track"

Sebastian Vettel has clarified the widely reported comments he made after Fernando Alonso's crash on the final day of the second Barcelona test

Fernando Alonso was left wondering what might have been after fighting through the field from last to sixth at the Monaco Grand Prix

Williams will launch a full investigation into the causes of its two crashes at the Monaco Grand Prix

A loose manhole cover was responsible for causing Rubens Barrichello's spectacular accident in the Monaco Grand Prix

Brave travellers can pick up a bargain on flights, in one of very few positives to come from the coronavirus outbreak.

Cases of motorists over the age of 70 surrendering licences rose from 6,612 in 2018 to 8,014 last year. That compared to a much smaller 10% rise in the 12 months previous.

Howard Cox, founder of FairFuelUK, said the average prices of a litre of unleaded and diesel should be 98p and 106p respectively, but are around 10p higher on average at retailers.

Kobe Bryant's daughter Natalia took to social media and honored the late coach John Altobelli on what would have been his first birthday since deadly helicopter crash in January.

Judge Louis Walsh was filming scenes with his act, Love Actually star Olivia Olson, on a London street when George, who proclaimed himself a huge fan of the IITV show, turned up.  

The former politician, who oversaw austerity in the years following the 2008 financial crisis, raised the spectre of banks refusing to lend money if businesses start going to the wall.

Don't text while driving as a new study suggests that doing so increases the risk of a crash much more than talking or listening on a mobile phone.

केंद्रीय उड्डयन मंत्री हरदीप सिंह पुरी के विदेश में फंसे भारतीयों को वापस लाने के बयान के बाद नागरिक उड्डयन मंत्रालय की वेबसाइट क्रैश हो गई। केंद्रीय मंत्री के बयान के बाद लाखों की संख्या में लोगों ने वेबसाइट पर विजिट

Sethi was reportedly rushing an injured friend to the hospital when she rammed into three cars.

This isn't quite the way Anthony McFarland expected his NFL career to begin. Pittsburgh's fourth-round draft pick is doing what he can to keep up during the first - and the NFL hopes only - ''virtual'' offseason amid the COVID-19 pandemic. Sure, he'd rather be at the Steelers' practice facility with the rest of the newcomers.

Most entering Madhya Pradesh from Maharashtra are headed to Uttar Pradesh.

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