A method of expanding and maintaining human embryonic stem cells (ESCs) in an undifferentiated state by culturing the ESCs in a suspension culture under culturing conditions devoid of substrate adherence is provided. Also provided are a method of deriving ESC lines in the suspension culture and methods of generating lineage-specific cells from ESCs which were expanded in the suspension culture of the present invention.

Thrombospondin 1 (TSP-1), TSP-2, interleukin 17B receptor (IL-17BR) and heparin-binding epidermal growth factor-like growth factor (HB-EGF) associated with stem cell activity and use thereof.

The present invention relates to the generation of a mucin-producing cell using stem/progenitor cells obtained from the amniotic membrane of umbilical cord and therapeutic uses of such mucin-producing cells.

Methods of washing adherent cells, capable of effectively suppressing cell death due to proteolytic enzyme treatment for detaching the adherent cell from a culture vessel and subsequent cell treatment; cell-washing solutions used for the washing method; methods of producing cell suspensions for transplantation using the cell-washing solution; and kits comprising the cell-washing solution. Trehalose or its derivative or a salt thereof is added to physiological aqueous solutions to prepare cell-washing solutions containing trehalose or its derivative or a salt thereof as an active ingredient. The cell-washing solutions can be used to wash adherent cells before detaching the adherent cells from a culture vessel by proteolytic enzyme treatment to suppress cell death due to the proteolytic enzyme treatment. The concentration of trehalose applied to the cell-washing solution may be a concentration capable of suppressing the cell death due to the proteolytic enzyme treatment, such as 0.1 to 20 (w/v) %.

Methods and devices for separating fluid-suspended plant somatic embryos and embryogenic tissue based on differences in their fluid drag properties are disclosed. Deposition method and device for depositing plant somatic embryos into embryo receiver comprising growth substrate by means of a fluid jet is disclosed. An automated system for processing plant somatic embryos from the bioreactor to the growth substrate is also disclosed.

Methods and compositions for targeted delivery of biotherapeutics are provided. The compositions comprise bile-sensitive St. thermophilus bacteria modified to release a biotherapeutic agent following bile exposure. Biotherapeutic agents released by the St. thermophilus bacteria disclosed herein include AQ and AQR rich peptides. Methods of the invention comprise administering to a subject a St. thermophilus bacterium modified to release a biotherapeutic agent following bile exposure. Administration of the St. thermophilus bacterium promotes a desired therapeutic response. The bacterium may be modified to express and release AQ or AQR rich peptides which subsequently inhibit cellular apoptosis or reduce mucosal damage. Thus, methods of the invention find use in treating or preventing a variety of gastrointestinal disorders including C. difficile infection and antibiotic-associated diarrhea.

A multi-channel system and methods for sorting particles according to one or more characteristics of the particles. The system includes multiple flow cytometry units, each unit can have a nozzle for producing a fluid stream containing a desired population of particles in a mixture of particles. Each of the units may be operable to sort said desired population of particles by interrogating the fluid stream with a beam of electromagnetic radiation and classifying particles based on one or more characteristics of the particles. The system also includes a common fluid delivery system for delivering sheath fluid to each flow cytometer unit for producing respective fluid streams.

The invention provides an improved design for the construction of extensible nucleic acid-based, ligand-controlled regulatory systems, and the nucleic acid regulatory systems resulting therefrom. The invention contemplates improving the design of the switches (ligand-controlled regulatory systems) through the design of an information transmission domain (ITD). The improved ITD eliminates free-floating ends of the switching and the competing strands, and localizes competitive hybridization events to a contiguous strand of competing and switching strands in a strand-displacement mechanism-based switch, thereby improving the kinetics of strand-displacement. The improved regulatory systems have many uses in various biological systems, including gene expression control or ligand-concentration sensing.

Methods and agents for reducing a level of an acetylated Tau polypeptide in a cell are provided. Methods for treating a tauopathy in an individual are also provided. Also provided is a method for diagnosing a cognitive impairment disorder in an individual. Methods for identifying an agent suitable for treating a tauopathy are also provided.

Disclosed are new members of a class of non-lipid small molecule inhibitors which interfere with the interaction between phosphoinositol-3,4,5-triphosphate (PIP3) and pleckstrin homology (PH) domains. These molecules target a broad range of PIP3-dependent signaling events in vitro and exert significant anti-tumor activity in vivo, with improved activity and selectivity toward particular PH domains. The small molecule inhibitors of the invention can be used alone or together with tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) or other cancer medicament to treat cancer. Small molecule inhibitors of the invention act synergistically in combination with TRAIL and with other Akt inhibitors in treating cancer. Pharmaceutical compositions and methods for treating cancer are provided.

The invention discloses novel morphology shifting micelles and amphiphilic coated metal nanofibers. Methods of using and making the same are also disclosed.

The invention is directed to a single-step method for rapidly and efficiently preparing protein-polymer conjugates, including an insulin-polymer conjugate. According to the method of the present invention, a protein and hydrophilic polymer are contacted in the presence of at least one organic solvent and at least one metal chelator, under conditions that promote the formation of a conjugate of the protein and polymer. Thus, the invention is directed to the site-specific modification of selected proteins, such as insulin, with poly(ethylene glycol) at residue PheB1. The invention also provides a pharmaceutical formulation for encapsulating the conjugate in a biodegradable polymer.

Monoclonal antibodies (mAbs) having thyroid stimulating activity (TSAb), especially full or considerably agonistic activity, or thyroid blocking activity (TBAb), which are obtainable by genetic immunization of mice, or fragments (F(ab')2, Fab or Fv) or humanized forms of such monoclonal antibodies or single chain forms (SCA; scFv) of such fragments, which antibodies, or their fragments, compete with bovine TSH for epitopes of the human TSHr, compete with autoantibodies from sera from Graves' patients as well as with autoantibodies from sera from patients harboring blocking autoantibodies for epitopes of the human TSHr, bind to conformational epitopes of the human TSHr located in the first 281 amino acids of the human TSHr, and usually also bind to TSFR receptors (TSHr) from different animals. Various uses of such antibodies, or of peptides corresponding to variable regions of such antibodies, are also described and claimed.

Devices and methods are provided for reducing matrix effects in protein precipitated bioanalytical samples comprising: a support, and a sorbent associated with the support capable of binding matrix interfering agents present in the bioanalytical sample, wherein the device further comprises filtering means for removing precipitated protein particles. The filtering means is a size exclusion filter or a polymeric or inorganic monolith having a maximum pore size less than or equal to the diameter of the particles to be removed from the sample, and can be integral with the sorbent or associated with the sorbent. The sorbent is characterized by sufficient selectivity between the matrix interfering agents and analytes of interest to provide retention of the matrix interfering agents while providing elution of the analytes of interest (e.g., a reversed phase or a polar modified reversed phase). Typical devices incorporating these features include luer syringe filters, individual filter cartridges, multiwell plates, pipette tips, or inline columns for multiple or single use.

Described herein are techniques for assembling a polynucleotide encoding a transcription activator-like effector nucleases (TALEN). The techniques ligate and digest necessary modules for a TALEN assembly in one reactor or system. Methods and Kits for generating a TALEN are also described.

The invention relates to methods and compositions for identifying and selecting maize plants with enhanced resistance to northern leaf blight. Maize plants generated by the methods of the invention are also a feature of the invention.

Isolated polynucleotides and polypeptides and recombinant DNA constructs particularly useful for altering agronomic characteristics of plants under nitrogen limiting conditions, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter functional in a plant, wherein said polynucleotide encodes an LNT1 polypeptide.

This invention provides recombinant DNA constructs and methods for manipulating expression of a target gene that is regulated by a small RNA, by interfering with the binding of the small RNA to its target gene. More specifically, this invention discloses recombinant DNA constructs encoding cleavage blockers, 5-modified cleavage blockers, and translational inhibitors useful for modulating expression of a target gene and methods for their use. Further disclosed are miRNA targets useful for designing recombinant DNA constructs including miRNA-unresponsive transgenes, miRNA decoys, cleavage blockers, 5-modified cleavage blockers, and translational inhibitors, as well as methods for their use, and transgenic eukaryotic cells and organisms containing such constructs.

The invention relates to genes coding for TCP family transcription factors and having a biological role in the development of axillary buds and branch growth. Furthermore, the invention relates to the promoters of the transcription of said genes, to the genetic constructs containing same and to the uses thereof, including the use of agents that modulate the expression of these genes in order to modify plant architecture.

A soybean cultivar designated 131TD735 is disclosed. The invention relates to the seeds of soybean cultivar 131TD735, to the plants of soybean 131TD735, to plant parts of soybean cultivar 131TD735 and to methods for producing a soybean plant produced by crossing soybean cultivar 131TD735 with itself or with another soybean variety. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. This invention also relates to soybean cultivars or breeding cultivars and plant parts derived from soybean cultivar 131TD735, to methods for producing other soybean cultivars, lines or plant parts derived from soybean cultivar 131TD735 and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants and plant parts produced by crossing the cultivar 131TD735 with another soybean cultivar.

A novel soybean variety, designated XB32T13 is provided. Also provided are the seeds of soybean variety XB32T13, cells from soybean variety XB32T13, plants of soybean XB32T13, and plant parts of soybean variety XB32T13. Methods provided include producing a soybean plant by crossing soybean variety XB32T13 with another soybean plant, methods for introgressing a transgenic trait, a mutant trait, and/or a native trait into soybean variety XB32T13, methods for producing other soybean varieties or plant parts derived from soybean variety XB32T13, and methods of characterizing soybean variety XB32T13. Soybean seed, cells, plants, germplasm, breeding lines, varieties, and plant parts produced by these methods and/or derived from soybean variety XB32T13 are further provided.

A novel soybean variety, designated XB52J13 is provided. Also provided are the seeds of soybean variety XB52J13, cells from soybean variety XB52J13, plants of soybean XB52J13, and plant parts of soybean variety XB52J13. Methods provided include producing a soybean plant by crossing soybean variety XB52J13 with another soybean plant, methods for introgressing a transgenic trait, a mutant trait, and/or a native trait into soybean variety XB52J13, methods for producing other soybean varieties or plant parts derived from soybean variety XB52J13, and methods of characterizing soybean variety XB52J13. Soybean seed, cells, plants, germplasm, breeding lines, varieties, and plant parts produced by these methods and/or derived from soybean variety XB52J13 are further provided.

A novel soybean variety, designated XB47X13 is provided. Also provided are the seeds of soybean variety XB47X13, cells from soybean variety XB47X13, plants of soybean XB47X13, and plant parts of soybean variety XB47X13. Methods provided include producing a soybean plant by crossing soybean variety XB47X13 with another soybean plant, methods for introgressing a transgenic trait, a mutant trait, and/or a native trait into soybean variety XB47X13, methods for producing other soybean varieties or plant parts derived from soybean variety XB47X13, and methods of characterizing soybean variety XB47X13. Soybean seed, cells, plants, germplasm, breeding lines, varieties, and plant parts produced by these methods and/or derived from soybean variety XB47X13 are further provided.

A novel maize variety designated PH1TWW and seed, plants and plant parts thereof. Methods for producing a maize plant that comprise crossing maize variety PH1TWW with another maize plant. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PH1TWW through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. Hybrid maize seed, plant or plant part produced by crossing the variety PH1TWW or a locus conversion of PH1TWW with another maize variety.

A novel maize variety designated PH1M9D and seed, plants and plant parts thereof. Methods for producing a maize plant that comprise crossing maize variety PH1M9D with another maize plant. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into PH1M9D through backcross conversion and/or transformation, and to the maize seed, plant and plant part produced thereby. Hybrid maize seed, plant or plant part produced by crossing the variety PH1M9D or a locus conversion of PH1M9D with another maize variety.

The present invention provides an inbred corn line designated FX6815, methods for producing a corn plant by crossing plants of the inbred line FX6815 with plants of another corn plant. The invention further encompasses all parts of inbred corn line FX6815, including culturable cells. Additionally provided herein are methods for introducing transgenes into inbred corn line FX6815, and plants produced according to these methods.

The present invention provides an inbred corn line designated FF7354, methods for producing a corn plant by crossing plants of the inbred line FF7354 with plants of another corn plant. The invention further encompasses all parts of inbred corn line FF7354, including culturable cells. Additionally provided herein are methods for introducing transgenes into inbred corn line FF7354, and plants produced according to these methods.

The present invention relates to a composition useful for the diagnosis of diseases associated with aberrant expression of the genes encoding the secreted proteins Futrin 1, 2, 3 and/or 4(=R-Spondin 2, 3, 1 and 4, respectively), e.g. in connection with tumors or diseases of the muscle, kidneys or bones. The present invention also relates to a pharmaceutical composition containing a compound which is capable of modifying (a) the expression of the gene encoding Futrin 1, 2, 3 and/or 4 or (b) the activity of the Futrin 1, 2, 3 and/or 4 protein.

The present invention comprises methods and systems for manipulation of media and particles, whether inert materials or biomaterials, such as cells in suspension cell culture. The methods and systems comprise use of an apparatus comprising a rotating chamber wherein the actions of the combined forces fluid flow force and centrifugal force form a fluidized bed within the rotating chamber.

The invention relates to compounds that specifically bind a T1R1/T1R3 or T1R2/T1R3 receptor or fragments or sub-units thereof. The present invention also relates to the use of hetero-oligomeric and chimeric taste receptors comprising T1R1/T1R3 and T1R2/T1R3 in assays to identify compounds that respectively respond to umami taste stimuli and sweet taste stimuli. Further, the invention relates to the constitutive of cell lines that stably or transiently co-express a combination of T1R1 and T1R3; or T1R2 and T1R3; under constitutive or inducible conditions. The use of these cells lines in cell-based assays to identify umami and sweet taste modulatory compounds is also provided, particularly high throughput screening assays that detect receptor activity by use of fluorometric imaging.

The present patent application concerns an enzyme capable of catalysing the conversion of a α-hydroxyglycine to an α-amide and the use of such enzymes for producing a C-terminal α-amidated peptide.

The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions.

An automated method for detecting the presence of a nucleic acid in a sample, where the method is performed within a housing of a self-contained, stand-alone analyzer. The method includes purifying the nucleic acid after it has been immobilized on a magnetically-responsive solid support. A pipette of the analyzer is used to form a reaction mixture comprising the purified nucleic acid and all reagents required to perform a nucleic acid amplification. Amplification products are synthesized that include a nucleotide sequence contained in the nucleic acid or the complement of the nucleic acid. The amplification products are exposed to a probe in a mixture, where the probe forms a hybrid with one of the amplification products. The formation of the hybrid in the mixture provides an indication of the presence of the nucleic acid in the sample.

In one aspect, there is provided a cell culturing substrate including: a cell culture surface having a film attached thereto, wherein the film includes one or more plasma polymerized monomers; and a coating on the film-coated surface, the coating deposited from a coating solution comprising one or more extracellular matrix proteins and an aqueous solvent, where the total extracellular matrix protein concentration in the coating solution is about 1 ng/mL to about 1 mg/mL.

This invention relates to the field of biotechnology or genetic engineering. Specifically, this invention relates to the field of gene expression. More specifically, this invention relates to novel substitution mutant receptors and their use in a nuclear receptor-based inducible gene expression system and methods of modulating the expression of a gene in a host cell for applications such as gene therapy, large scale production of proteins and antibodies, cell-based high throughput screening assays, functional genomics and regulation of traits in transgenic organisms.

Provided herein are methods for making water-soluble nanoparticles comprising a core/shell nanocrystal that is coated with a surface layer comprising enough hydrophilic ligands to render the nanoparticle water soluble or water dispersable. Methods for crosslinking molecules on the surface of a nanoparticle, which methods can be used on the above water-soluble nanoparticles also are provided. Nanoparticle compositions resulting from these methods are also provided.

Disclosed are process for contacting a protein containing material with one or more wet-mill streams. The protein content of the protein containing material is increased.

The invention relates to malodor controlling bacteria and related methods and compositions for the control and prevention of malodor.

The present invention relates, in general, to gene expression and, in particular, to a method of inhibiting the expression of a target gene and to constructs suitable for use in such a method.

[Problem to be Solved] The importance of sugar chains having α2,3- or α2,6-linked sialic acid at their non-reducing ends is known. Industrial production has been demanded for these sugar chain compounds. Particularly, the production of glycoprotein drugs or the like inevitably requires producing in quantity sugar chains having homogeneous structures by controlling the linking pattern (α2,6-linkage or α2,3-linkage) of sialic acid. Particularly, a triantennary or tetraantennary N-type complex sugar chain having sialic acid at each of all non-reducing ends is generally considered difficult to chemically synthesize. There has been no report disclosing that such a sugar chain was chemically synthesized. Furthermore, these sugar chains are also difficult to efficiently prepare enzymatically.[Solution]The present inventors have newly found the activity of sialyltransferase of degrading sialic acid on a reaction product in the presence of CMP and also found that formed CMP can be degraded enzymatically to thereby efficiently produce a sialic acid-containing sugar chain. The present inventors have further found that even a tetraantennary N-type sugar chain having four α2,6-linked sialic acid molecules, which has previously been difficult to synthesize, can be prepared at high yields by one-pot synthesis comprising the elongation reaction of a biantennary sugar chain used as a starting material without performing purification after each enzymatic reaction.

A chair assembly includes a four-bar linkage assembly including a first linkage member, a second linkage member, a third linkage member and a fourth linkage member each pivotably coupled to one another such that the four-bar linkage assembly includes an upper end that is adjustable between raised and lowered positions, and an arm rest assembly adapted to support the arm of a seated user thereon and supported the upper end of the four-bar linkage assembly, wherein a lower end of the four-bar linkage assembly is pivotably supported from an arm support structure for pivotable movement, such that the upper end of the four-bar linkage assembly is moveable between a first position and second position located laterally outward from the first position.

A vehicle seat can include a seat bottom frame having a front, a back opposite the front, and enclosing a volume. Pivotally connected to the back of the seat bottom frame can be a seat back frame and pivotally connected to the front of the seat bottom frame can be a seat leg rest. The seat leg rest has a length. Mounted in the volume of the seat frame are at least two leg rest springs which are connected to the leg rest. When the leg rest springs are actuated, the seat leg rest can be directly pivoted approximately and incrementally parallel with the seat bottom frame. The two leg rest springs are mounted a first distance apart and the first distance is less than half of the length of the leg rest.

A stylish ready-to-assemble chaise lounge and sofa which may be easily and quickly assembled by a consumer, is provided. The invention is a kit from which either a stylish chaise lounge or a sofa may be easily assembled by a consumer using a single Allen wrench. The kits, both chaise and sofa, comprise a seat base, a long side seatback and a short side seatback, feet, fasteners and cushions. With regard to the chaise, the long and short side chaise seatbacks have rectangular lower portions with upper portions featuring a flowing curved “waterfall” seating surface which improves the comfort of a user leaning back against the seatback.

A method for enhancing sales of stadium seat memorabilia by utilizing a stabilizing base, with a large surface area in the shape of sports memorabilia, such as baseball bats and/or sports figures, with or without brackets, to mount and stabilize a stadium seat for use outside of the stadium, such as at home or in a commercial setting.

A vehicle seat has a longitudinal adjuster, a pair of seat rails, a releasable latching device having at least one actuating pin that projects over the second seat rail, a pivotably arranged actuating lever, and a Bowden cable. A holder is fixed on a rail and supports the actuating lever such as to be pivotable about a pivot axis at one end, and a contact surface is formed on the actuating lever, on which surface the core of the Bowden cable rests in a subarea between the end of the sheath and the end of the core. The holder secures the end of the core of the Bowden cable, and the actuating lever has a Bowden cable support on which the sheath of the Bowden cable is supported directly or indirectly.

A folding chair capable of being moved between a folded position and an unfolded position, includes: two units, each of which includes first and second tubes hinged together via a hinge pin; and a canvas sheet, where each assembly of two tubes includes a rigid part at the hinge pin thereof, said rigid part being arranged between two tubes and having: a groove for receiving a tube of the assembly without any degree of freedom; first and second cradles arranged on either side of the hinge pin, such that when the chair is in the unfolded position, the first cradle bears against the upper section of the other tube and the second cradle bears against the lower section of the other tube.

A chair including a dynamically-flexible back and seat having a shape that adjusts to the movements of a user seated in the chair to enhance user comfort. Each of the back and seat has an air gap formed therewithin and a resilient frame or liner with a spring memory. The resilient liner is adapted to flex in response to compressive forces that are generated as the user slides his body back or from side-to-side in the chair. Accordingly, some of the chair back and some of the chair seat move into respective air gaps so that the shapes of the back and seat change to conform to the movements of the user. The air gaps also establish air flow ventilation channels which run laterally through the back and seat of the chair.

A premium class passenger suite that includes a main seat positioned in the suite together with separate bed. The bed has a flexible mattress of predetermined dimensions and is movable between a stowed position to one side of the main seat and a deployed position above and separate from the main seat. A drive apparatus is provided for driving the bed between the stowed and deployed positions. The main seat is configured to be movable between a seating position when the bed is stowed, and a stowed position with a lowered seat back when the bed is deployed for use.

A chair for facilitating efficient stacking includes a seat element having a seat frame, a plurality of front legs attached to a front portion of the seat element, a plurality of rear legs attached to a back portion of the seat element, and one or more telescopic leg mechanisms disposed on at least one of the front or rear legs, wherein the telescopic leg mechanisms provide for lateral movement of the at least one of the front or rear legs with respect to the seat element.

An infant rocking seat includes a base; a track provided on the base having a first arc-shaped portion and a second arc-shaped portion meeting at a crest; a carriage having a body portion, a first pair of wheels positioned at a first end of the body portion, and a second pair of wheels positioned at a second end of the body portion; and a drive mechanism configured to move the carriage along the track. The carriage is positioned within a central portion of the base and is configured to ride along the track. A distance between the first pair of wheels and the second pair of wheels is less than a distance between centers of curvature of the first arc-shaped portion and the second arc-shaped portion.