liz Sources: MLB finalizing return-to-play proposal By www.espn.com Published On :: Wed, 6 May 2020 20:46:42 EST MLB expects to offer a return-to-play proposal to the players' union within a week, as teams have begun to encourage players to prepare for a "spring" training that could begin in mid-June and a season that could start in early July, sources told ESPN. Full Article
liz DAC 2015: How Academia and Industry Collaboration Can Revitalize EDA By feedproxy.google.com Published On :: Wed, 17 Jun 2015 21:14:00 GMT Let’s face it – the EDA industry needs new people and new ideas. One of the best places to find both is academia, and a presentation at the Cadence Theater at the recent Design Automation Conference (DAC 2015) described collaboration models that are working today. The presentation was titled “Industry/Academia Engagement Models – From PhD Contests to R&D Collaborations.” It included these speakers, shown from left to right in the photo below: Prof. Xin Li, Electrical and Computer Engineering, Carnegie-Mellon University (CMU) Chuck Alpert, Senior Software Architect, Cadence Prof. Laleh Behjat, Department of Electrical and Computer Engineering, University of Calgary Alpert, who was filling in for Zhuo Li, Software Architect at Cadence, was the vice chair of DAC 2015 and will be the general chair of DAC 2016 in Austin, Texas. “My team at Cadence really likes to collaborate with universities,” he said. “We’re a big proponent of education because we really need the best and brightest students in our industry.” Contests Boost EDA Research One way that Cadence collaborates with academia is participation in contests. “It’s a great way to formulate problems to academia,” Alpert said. “We can have the universities work on these problems and get some strategic direction.” For example, Cadence has been involved with the annual CAD contest at the International Conference on Computer-Aided Design (ICCAD) since the contest was launched in 2012. This is the largest worldwide EDA R&D contest, and it is sponsored by the IEEE Council on EDA (CEDA) and the Taiwan Ministry of Education. Its goals are to boost EDA research in advanced real-world problems and to foster industry-academia collaboration. Contestants can participate in one of more problems in the three areas of system design, logic synthesis and verification, and physical design. The 2015 contest has attracted 112 teams from 12 regions. Cadence contributes one problem per year in the logic synthesis area. Zhuo Li was the 2012 co-chair and the 2013 chair. The awards will be given at ICCAD in November 2015. Another step that Cadence has taken, Alpert said, is to “hire lots of interns.” His own team has four interns at the moment. One advantage to interning at Cadence, he said, is that students get to see real-world designs and understand how the tools work. “It helps you drive your research in a more practical and useful direction,” he said. The Cadence Academic Network co-sponsors the ACM SIGDA PhD Forum at DAC, and Xin Li and Zhuo Li are on the organizing committee. This event is a poster session for PhD students to present and discuss their dissertation research with people in the EDA community. This year’s forum was “packed,” Alpert said, and it’s clear that the event needs a bigger room. Finally, Alpert noted, Cadence researchers write and publish technical papers at DAC and other conferences, and Cadence people serve on the DAC technical program committee. “We try to be involved with the academic community on a regular basis,” Alpert said. “We want the best and the brightest people to go into EDA because there is still so much innovation that’s needed. It’s a really cool place to be.” Research Collaboration Exposes Failure Rates Xin Li presented an example of a successful research collaboration between CMU and Cadence. The challenge was to find a better way to estimate potential failure rates in memory. As noted in a previous blog post, PhD student Shupeng Sun met this challenge with a new statistical methodology that won a Best Poster award at the ACM SIGDA PhD Forum at DAC 2014. The new methodology is called Scaled-Sigma Sampling (SSS). It calculates the failure rate and accounts for variability in the manufacturing process while only requiring a few hundred, or a few thousand, sample circuit blocks. Previously, millions of samples were required for an accurate validation of a new design, and each sample could take minutes or hours to simulate. It could take a few weeks or months to run one validation. The SSS methodology requires greatly reduced simulation times. It makes it possible, Li noted, to run simulations overnight and see the results in the morning. Li shared his secret for success in collaborations. “I want to emphasize that before the collaboration, you have to understand the goal. If you don’t have a clear goal, don’t collaborate. Once you define the goal, stick to it and make it happen.” Contest Provides Learning Experience Last year Laleh Behjat handed two of her new PhD students a challenge. “I told them there is an ISPD [International Symposium for Physical Design] contest on placement, and I expect you to participate and I expect you to win. Not knowing anything about placement, I don’t think they realized what I was asking them.” The 2015 contest was called the Blockage-Aware Detailed Routing-Driven Placement Contest. Results were announced at the end of March at ISPD. And the University of Calgary team, despite its lack of placement experience, took second place. Such contests provide a good learning tool, according to Behjat. Graduate students in EDA, she said, “have to be good programmers. They have to work in teams and be collaborative, be able to innovate, and solve the hardest problems I have seen in engineering and science. And they have to think outside the box.” A contest can bring out all these attributes, she said. Further, Behjat noted, contest participants had access to benchmarks and to a placement tool. They didn’t have to write tools to find out if their results were good. Industry sponsors, meanwhile, got access to good students and new approaches for solving problems. “You can see Cadence putting a big amount of time, effort and money to get students here and get them excited about doing contests,” she said. She advised students in the theater audience to “talk to people in the Cadence booth and see if you can have more ideas for collaboration.” Richard Goering Related Blog Posts EDA Plus Academia: A Perfect Game, Set and Match Cadence Aims to Strengthen Academic Partnerships BSIM-CMG FinFET Model – How Academia and Industry Empowered the Next Transistor Full Article ISPD Cadence Academic Network academia-industry collaboration ICCAD DAC 2015 scaled-sigma sampling PhD Forum EDA contests
liz Attackers Capitalizing On Unpatched WordPress Sites By packetstormsecurity.com Published On :: Wed, 08 Feb 2017 15:24:05 GMT Full Article headline hacker data loss flaw wordpress
liz Singapore Government Must Realize Human Error Also A Security Breach By packetstormsecurity.com Published On :: Fri, 29 Nov 2019 16:54:39 GMT Full Article headline government privacy data loss singapore
liz CES 2020 Survey by CITE Research/Dassault Systèmes: Consumers Want Personalized Products but Won’t Wait for Them and Expect a Cost Benefit for Their Data By www.3ds.com Published On :: Mon, 06 Jan 2020 00:19:33 +0100 •Survey of 3,000 consumers in the U.S., China and France examines views on personalization in healthcare, mobility, retail, and home and city environments •Personalization in healthcare, prioritized over other categories, will require AI, 5G and home assistant technologies to achieve, according to respondents •Consumers will pay on average 25.3% more for personalization, but they expect a savings in return •Generation X, millennials, and Generation Z are more willing to pay and share data... Full Article 3DEXPERIENCE High-Tech Home & Lifestyle Transportation & Mobility Life Sciences Events
liz Would-Be Hacker Vandalizes Vietnam Memorial Site By packetstormsecurity.com Published On :: Thu, 20 Sep 2007 12:17:01 GMT Full Article hacker vietnam
liz John McAfee Now Claims He Has A Spy Ring In The Belize Government By packetstormsecurity.com Published On :: Tue, 08 Jan 2013 04:31:22 GMT Full Article headline government spyware mcafee
liz McAfee Claims Ashley Madison Hack Could Destabilize Society By packetstormsecurity.com Published On :: Fri, 24 Jul 2015 14:24:46 GMT Full Article headline hacker privacy data loss mcafee
liz John McAfee Found Liable For 2012 Death Of Belize Neighbor By packetstormsecurity.com Published On :: Fri, 16 Nov 2018 15:27:55 GMT Full Article headline mcafee
liz Dismantling Megamos Crypto: Wirelessly Lockpicking A Vehicle Immobilizer By packetstormsecurity.com Published On :: Fri, 14 Aug 2015 13:02:14 GMT The Megamos Crypto transponder is used in one of the most widely deployed electronic vehicle immobilizers. It is used among others in most Audi, Fiat, Honda, Volkswagen and Volvo cars. Such an immobilizer is an anti-theft device which prevents the engine of the vehicle from starting when the corresponding transponder is not present. This transponder is a passive RFID tag which is embedded in the key of the vehicle. In this paper, the authors have reverse-engineered all proprietary security mechanisms of the transponder, including the cipher and the authentication protocol which we publish here in full detail. This article reveals several weaknesses in the design of the cipher, the authentication protocol and also in their implementation. Full Article
liz Furukawa Electric ConsciusMAP 2.8.1 Java Deserialization Remote Code Execution By packetstormsecurity.com Published On :: Fri, 24 Apr 2020 14:40:45 GMT Furukawa Electric ConsciusMAP version 2.8.1 java deserialization remote code execution exploit. Full Article
liz KVM kvm_inject_page_fault Uninitialized Memory Leak By packetstormsecurity.com Published On :: Sat, 16 Feb 2019 14:53:29 GMT KVM suffers from an uninitialized memory leak vulnerability in kvm_inject_page_fault. Full Article
liz Quest InTrust Annotation Objects Uninitialized Pointer By packetstormsecurity.com Published On :: Fri, 13 Apr 2012 22:12:21 GMT This Metasploit module exploits an uninitialized variable vulnerability in the Annotation Objects ActiveX component. The activeX component loads into memory without opting into ALSR so this module exploits the vulnerability against windows Vista and Windows 7 targets. A large heap spray is required to fulfill the requirement that EAX points to part of the ROP chain in a heap chunk and the calculated call will hit the pivot in a separate heap chunk. This will take some time in the users browser. Full Article
liz EPA finalizes Affordable Clean Energy rule, repeals Clean Power Plan By feedproxy.google.com Published On :: 2019-06-20T14:58:00Z The U.S. Environmental Protection Agency (EPA) has issued the Affordable Clean Energy (ACE) rule and simultaneously repealed the Clean Power Plan (CPP). Full Article Environmental North America Government and Policy News News Hydropower Storage Wind Power Solar Geothermal
liz Houston Mayor selects developer for 70-MW urban solar array in revitalization plan By feedproxy.google.com Published On :: 2019-09-03T15:06:08Z Last week, Houston, TX Mayor Sylvester Turner announced that Sunnyside Energy, led by developer Dori Wolfe of Wolfe Energy LLC, has won a competition to be considered to repurpose a 240-acre former landfill in Sunnyside. Subject to meeting certain terms and conditions, the team will construct one of the largest urban solar farms in Texas., if not the largest. Full Article DER News Utility Integration Community Solar
liz Goldman Sachs sees solar panel prices stabilizing By feedproxy.google.com Published On :: 2019-05-01T15:22:50Z Goldman Sachs Group Inc. sees an end to the gloom for solar manufacturers as demand in China stabilizes and free-falling panel prices bottom out. Full Article Solar Storage News
liz EPA finalizes Affordable Clean Energy rule, repeals Clean Power Plan By feedproxy.google.com Published On :: 2019-06-20T14:58:00Z The U.S. Environmental Protection Agency (EPA) has issued the Affordable Clean Energy (ACE) rule and simultaneously repealed the Clean Power Plan (CPP). Full Article Environmental North America Government and Policy News News Hydropower Storage Wind Power Solar Geothermal
liz How Digitalization and Blockchain Can Unlock an Energy Trading Revolution By feedproxy.google.com Published On :: 2018-11-10T02:45:00Z Digital solutions could revolutionize energy trading if they were adopted more widely across the market. Full Article Digitalization Energy Efficiency Big Data Strategic Development Solar Utility Integration
liz OEE 2015 builds on 2014 market study to commercialize MHK energy sectors By feedproxy.google.com Published On :: 2015-11-16T21:37:00Z During the Ocean Energy Europe 2015 conference held in Dublin, Ireland, in late October, about 500 high-level delegates, which included European Union and Member States business leaders and energy ministers, were presented a draft of the “strategic roadmap” for developing the European marine hydrokinetics (MHK) energy sector. Full Article
liz Papua New Guinea First to Finalize Climate Plan Under Paris Agreement By feedproxy.google.com Published On :: 2016-04-08T16:48:00Z Papua New Guinea recently became the first country to formally submit the final version of its national climate action plan (called a “Nationally Determined Contribution,” or NDC) under the Paris Agreement. The small Pacific nation’s plan to transition to 100 percent renewable energy by 2030 is no longer just an “intended” nationally determined contribution (INDC) — it is now the country’s official climate plan. Full Article Hydropower Baseload Bioenergy Wind Power Solar Geothermal
liz Goldman Sachs sees solar panel prices stabilizing By feedproxy.google.com Published On :: 2019-05-01T15:22:50Z Goldman Sachs Group Inc. sees an end to the gloom for solar manufacturers as demand in China stabilizes and free-falling panel prices bottom out. Full Article Solar Storage News
liz EPA finalizes Affordable Clean Energy rule, repeals Clean Power Plan By feedproxy.google.com Published On :: 2019-06-20T14:58:00Z The U.S. Environmental Protection Agency (EPA) has issued the Affordable Clean Energy (ACE) rule and simultaneously repealed the Clean Power Plan (CPP). Full Article Environmental North America Government and Policy News News Hydropower Storage Wind Power Solar Geothermal
liz Microgrids Create Municipalization Benefits By feedproxy.google.com Published On :: 2014-06-02T10:39:00Z Electric utilities seeking the renewal of their franchises, and politicians seeking municipalization, both ignore the transformative possibilities of the microgrid. Microgrids represent a promising new business opportunity for both existing utilities and new entrants in the electricity business. Microgrid deployment will also provide the same public benefits as municipal control, likely more. Full Article Microgrids Microgrids Opinion & Commentary Solar Storage Grid Scale
liz March 25, 2020 - IPC Welcomes U.S. Economic Stabilization Package, Proposes Agenda for Economic Recovery and Resiliency in Electronics Manufacturing By www.ipc.org Published On :: Full Article
liz Digitalizing Wind Power: Myths, Challenges and Approaches By feedproxy.google.com Published On :: 2019-01-22T15:41:48Z Digitalization has become a worldwide focus and the race to become the global leader in Artificial Intelligence (AI) is becoming increasingly competitive, with many countries, including Canada, China, and the UK releasing strategies in the last twenty months to promote the use and development of AI. Full Article Monitoring Onshore O&M Wind Power Opinion & Commentary Project Development Asset Management Offshore
liz EPA finalizes Affordable Clean Energy rule, repeals Clean Power Plan By feedproxy.google.com Published On :: 2019-06-20T14:58:00Z The U.S. Environmental Protection Agency (EPA) has issued the Affordable Clean Energy (ACE) rule and simultaneously repealed the Clean Power Plan (CPP). Full Article Environmental North America Government and Policy News News Hydropower Storage Wind Power Solar Geothermal
liz Papua New Guinea First to Finalize Climate Plan Under Paris Agreement By feedproxy.google.com Published On :: 2016-04-08T16:48:00Z Papua New Guinea recently became the first country to formally submit the final version of its national climate action plan (called a “Nationally Determined Contribution,” or NDC) under the Paris Agreement. The small Pacific nation’s plan to transition to 100 percent renewable energy by 2030 is no longer just an “intended” nationally determined contribution (INDC) — it is now the country’s official climate plan. Full Article Hydropower Baseload Bioenergy Wind Power Solar Geothermal
liz Internationalizing the Yuan By feedproxy.google.com Published On :: Thu, 07 Apr 2011 08:35:29 +0000 Internationalizing the Yuan A Hong Kong bond issue is a small but important step By Christopher A. McNally (Note: This commentary originally appeared in The Walt Street Journal's Asia edition on Sept. 10, 2009) Beijing's announcement earlier this week that it will sell yuan-denominated government bonds in Hong Kong has caught a lot of attention. The Ministry of Finance bills it as a measure to "promote the yuan in neighboring countries and improve the yuan's international status." The move comes in the context of Beijing's larger goal of transforming the yuan into a global currency. While on its own this is a relative baby step, it is more significant than at first appears. Full Article
liz South Korean Teachers Visiting U.S. Schools to Share Globalized Teaching Practices By feedproxy.google.com Published On :: Fri, 10 Jan 2014 20:03:22 +0000 HONOLULU (Jan. 10, 2014) – Twenty K-12 educators from South Korea have arrived at the East-West Center to begin a month of residencies in U.S. school communities in Connecticut, New York, Massachusetts and Vermont. The ROK-US Teacher Exchange Program global learning and school immersion program is coordinated by EWC’s AsiaPacificEd Program with funding from the Asia-Pacific Centre of Education for International Understanding under the auspices of UNESCO and the Republic of Korea’s Ministry of Education. Later this year, American teachers from the U.S. host schools will travel to Korea for reciprocal exchange and learning. Full Article
liz Future of Our Global Economy: The Beginning of De-Globalization By www.spiegel.de Published On :: Tue, 5 May 2020 10:46:49 +0200 The corona crisis is changing the global economy. Production security is growing more important than efficiency. Here is what that might look like. Full Article
liz Queen Elizabeth Honours War Dead: 'They Died So We Could Live as Free People' By article.wn.com Published On :: Sat, 09 May 2020 15:12 GMT Queen Elizabeth II marked the 75th anniversary of Victory in Europe Day with an address to the British Commonwealth honouring the sacrifice of the fallen. ...... Full Article
liz Queen Elizabeth II addresses UK on 75th VE Day By www.hurriyetdailynews.com Published On :: Sat, 09 May 2020 07:49:37 Z Queen Elizabeth II addressed the UK and Commonwealth on May 8 for the 75th anniversary of Victory in Europe Day that marks the end of World War II in Europe. Full Article World
liz Turkey 'neutralizes' over 1,300 terrorists in 2020 By www.hurriyetdailynews.com Published On :: Sat, 09 May 2020 11:34:54 Z Turkey has neutralized over 1,300 terrorists across five different operation areas since the beginning of 2020, the country's national defense minister said. Full Article Turkey
liz Israel's IIBR finds antibody that neutralizes coronavirus By www.jpost.com Published On :: Tue, 05 May 2020 17:51:11 GMT IIBR, Yeroham to collaborate on vaccine production facility Full Article vaccine coronavirus outbreak Coronavirus in Israel Coronavirus spread
liz Syrian man vandalizes Amsterdam kosher restaurant for second time By www.jpost.com Published On :: Fri, 08 May 2020 23:53:16 GMT The attack was the fifth case of vandalism or intimidation in 2 ½ years. Full Article Syria antisemitism Holland
liz Caruana Galizia murder suspect on hunger strike at ‘discriminatory’ court sitting By www.maltatoday.com.mt Published On :: Sat,09 May 2020 10:29:47 +0200 Murder suspect Alfred Degiorgio does not want to appear in court after COVID-19 lockdown prevented him from seeing family Full Article
liz At your service: trade liberalization could bring huge benefits to Southeast Asia -- by Kakali Mukhopadhyay By blogs.adb.org Published On :: Fri, 18 Oct 2019 11:04:18 +0800 Making it easier for workers to move between countries is key to liberalizing the trade in services and unleashing the benefits it will produce across national and regional economies. Full Article
liz Visualizing Square Roots with Elias Wegert By feedproxy.google.com Published On :: Wed, 11 Mar 2020 15:03:07 +0000 Quick, what’s the square root of -1? Okay, I know. That’s an easy one. But how about the square root of i? If it’s been a while since you took complex analysis, you might have to scratch your head a little bit. Fortunately, MATLAB can just tell us. ... read more >> Full Article Uncategorized
liz Tiny bird-like dinosaur discovered in amber might actually be a lizard By www.newscientist.com Published On :: Wed, 01 Apr 2020 10:00:53 +0000 A 99-million-year-old skull recently discovered in amber might actually belong to a lizard, rather than a tiny bird-like dinosaur as first thought Full Article
liz The 2025 User Experience: Predictions for the Future of Personalized Technology By news.harman.com Published On :: Mon, 03 Jun 2019 13:30:00 GMT History is witness to the many scientific leaders and technology visionaries who all tried to predict what innovations will exist in the future. While not all predictions come to fruition, others were not so far off. We may not have flying cars like The... Full Article
liz HARMAN ExP: Integrated Technologies that Boost Personalized Experiences By news.harman.com Published On :: Fri, 13 Mar 2020 14:00:00 GMT In an industry driven by incremental technology advancements, droning on from one model year to the next, automotive innovation can sometimes stall out. Functionally, and spiritually, what was created to move you, doesn’t deliver as advertised. As a... Full Article
liz omalizumab (Xolair) By www.medicinenet.com Published On :: Fri, 15 Nov 2019 00:00:00 PDT Title: omalizumab (Xolair)Category: MedicationsCreated: 5/27/2004 12:00:00 AMLast Editorial Review: 11/15/2019 12:00:00 AM Full Article
liz Smoke-Free Laws May Help Prevent COPD Hospitalizations By www.medicinenet.com Published On :: Sat, 9 May 2020 00:00:00 PDT Title: Smoke-Free Laws May Help Prevent COPD HospitalizationsCategory: Health NewsCreated: 4/25/2014 2:35:00 PMLast Editorial Review: 4/28/2014 12:00:00 AM Full Article
liz 1 in 4 Hospitalized Newborns Gets Heartburn Drugs, Despite Risks By www.medicinenet.com Published On :: Sat, 9 May 2020 00:00:00 PDT Title: 1 in 4 Hospitalized Newborns Gets Heartburn Drugs, Despite RisksCategory: Health NewsCreated: 4/27/2016 12:00:00 AMLast Editorial Review: 4/28/2016 12:00:00 AM Full Article
liz Pneumonia More Deadly Than Hip Fractures for Hospitalized Seniors By www.medicinenet.com Published On :: Fri, 24 Apr 2020 00:00:00 PDT Title: Pneumonia More Deadly Than Hip Fractures for Hospitalized SeniorsCategory: Health NewsCreated: 4/23/2020 12:00:00 AMLast Editorial Review: 4/24/2020 12:00:00 AM Full Article
liz Entyvio (vedolizumab) By www.medicinenet.com Published On :: Fri, 17 Apr 2020 00:00:00 PDT Title: Entyvio (vedolizumab)Category: MedicationsCreated: 4/17/2020 12:00:00 AMLast Editorial Review: 4/17/2020 12:00:00 AM Full Article
liz Measuring Oral Health Literacy of Refugees: Associations with Dental Care Utilization and Oral Health Self-Efficacy By jdh.adha.org Published On :: 2020-04-30T12:39:03-07:00 Purpose: The purpose of this study was to analyze associations between the oral health literacy of refugees and two oral health outcomes: dental care utilization and oral health self-efficacy.Methods: A convenience sample of refugees in the greater Los Angeles area attending English as a second language (ESL) classes sponsored by two refugee assistance organizations was used for this cross-sectional, correlational study. Participants responded to a questionnaire using items from the Health Literacy in Dentistry (HeLD) scale, in addition to items concerning dental care utilization and oral health self-efficacy. Descriptive statistics, chi-square and Fisher's Exact tests were used to analyze results.Results: Sixty-two refugees volunteered to participate (n=62). A majority of the respondents were female from Iraq or Syria, and selected the item “with little difficulty” for all oral health literacy tasks. In regards to dental care utilization, more than half of the respondents were considered high utilizers (63%, n=34) meaning they had visited a dental office within the last year; while a little more than one-third (37%, n=20), were low utilizers, indicating they had either never been to a dental office or it had been more than one year since they had dental treatment. Statistical analysis showed associations between oral health literacy and dental care utilization. However, few associations between oral health literacy and oral health self-efficacy were identified (p=0.0045).Conclusions: Results support the provision of easily obtainable and understandable oral health information to increase oral health literacy and dental care utilization among refugee populations. Future research is needed to examine the oral health literacy among refugees resettling in the United States. Full Article
liz Maternal Broadly Neutralizing Antibodies Can Select for Neutralization-Resistant, Infant-Transmitted/Founder HIV Variants By mbio.asm.org Published On :: 2020-03-10T01:30:41-07:00 ABSTRACT Each year, >180,000 infants become infected via mother-to-child transmission (MTCT) of HIV despite the availability of effective maternal antiretroviral treatments, underlining the need for a maternal HIV vaccine. We characterized 224 maternal HIV envelope (Env)-specific IgG monoclonal antibodies (MAbs) from seven nontransmitting and transmitting HIV-infected U.S. and Malawian mothers and examined their neutralization activities against nontransmitted autologous circulating viruses and infant-transmitted founder (infant-T/F) viruses. Only a small subset of maternal viruses, 3 of 72 (4%), were weakly neutralized by maternal linear V3 epitope-specific IgG MAbs, whereas 6 out of 6 (100%) infant-T/F viruses were neutralization resistant to these V3-specific IgG MAbs. We also show that maternal-plasma broadly neutralizing antibody (bNAb) responses targeting the V3 glycan supersite in a transmitting woman may have selected for an N332 V3 glycan neutralization-resistant infant-T/F virus. These data have important implications for bNAb-eliciting vaccines and passively administered bNAbs in the setting of MTCT. IMPORTANCE Efforts to eliminate MTCT of HIV with antiretroviral therapy (ART) have met little success, with >180,000 infant infections each year worldwide. It is therefore likely that additional immunologic strategies that can synergize with ART will be required to eliminate MTCT of HIV. To this end, understanding the role of maternal HIV Env-specific IgG antibodies in the setting of MTCT is crucial. In this study, we found that maternal-plasma broadly neutralizing antibody (bNAb) responses can select for T/F viruses that initiate infection in infants. We propose that clinical trials testing the efficacy of single bNAb specificities should not include HIV-infected pregnant women, as a single bNAb might select for neutralization-resistant infant-T/F viruses. Full Article
liz The Pseudomonas aeruginosa Lectin LecB Causes Integrin Internalization and Inhibits Epithelial Wound Healing By mbio.asm.org Published On :: 2020-03-10T01:30:42-07:00 ABSTRACT The opportunistic bacterium Pseudomonas aeruginosa produces the fucose-specific lectin LecB, which has been identified as a virulence factor. LecB has a tetrameric structure with four opposing binding sites and has been shown to act as a cross-linker. Here, we demonstrate that LecB strongly binds to the glycosylated moieties of β1-integrins on the basolateral plasma membrane of epithelial cells and causes rapid integrin endocytosis. Whereas internalized integrins were degraded via a lysosomal pathway, washout of LecB restored integrin cell surface localization, thus indicating a specific and direct action of LecB on integrins to bring about their endocytosis. Interestingly, LecB was able to trigger uptake of active and inactive β1-integrins and also of complete α3β1-integrin–laminin complexes. We provide a mechanistic explanation for this unique endocytic process by showing that LecB has the additional ability to recognize fucose-bearing glycosphingolipids and causes the formation of membrane invaginations on giant unilamellar vesicles. In cells, LecB recruited integrins to these invaginations by cross-linking integrins and glycosphingolipids. In epithelial wound healing assays, LecB specifically cleared integrins from the surface of cells located at the wound edge and blocked cell migration and wound healing in a dose-dependent manner. Moreover, the wild-type P. aeruginosa strain PAO1 was able to loosen cell-substrate adhesion in order to crawl underneath exposed cells, whereas knockout of LecB significantly reduced crawling events. Based on these results, we suggest that LecB has a role in disseminating bacteria along the cell-basement membrane interface. IMPORTANCE Pseudomonas aeruginosa is a ubiquitous environmental bacterium that is one of the leading causes of nosocomial infections. P. aeruginosa is able to switch between planktonic, intracellular, and biofilm-based lifestyles, which allows it to evade the immune system as well as antibiotic treatment. Hence, alternatives to antibiotic treatment are urgently required to combat P. aeruginosa infections. Lectins, like the fucose-specific LecB, are promising targets, because removal of LecB resulted in decreased virulence in mouse models. Currently, several research groups are developing LecB inhibitors. However, the role of LecB in host-pathogen interactions is not well understood. The significance of our research is in identifying cellular mechanisms of how LecB facilitates P. aeruginosa infection. We introduce LecB as a new member of the list of bacterial molecules that bind integrins and show that P. aeruginosa can move forward underneath attached epithelial cells by loosening cell-basement membrane attachment in a LecB-dependent manner. Full Article
liz Erratum for Townsend et al., "A Master Regulator of Bacteroides thetaiotaomicron Gut Colonization Controls Carbohydrate Utilization and an Alternative Protein Synthesis Factor" By mbio.asm.org Published On :: 2020-03-17T01:30:14-07:00 Full Article