The National Cherry Blossom Festival has moved online due to the novel coronavirus pandemic

Built nearly 4,700 years ago as a tomb for the pharaoh Djoser, the structure underwent more than a decade of on-and-off restorations

The new findings raises questions about the authenticity of a collection of texts known as the "post-2002" scrolls

New research reveals a Tang Dynasty woman's love for sports—and big-eared, braying equids

But will that keep susceptible populations safe?

The puzzling glyph, which bears some resemblance to the "squatting man" motif, suggests that insects have long held a place in human lore

The findings further refute the outdated notion that humans' capacity for complex artistic expression evolved exclusively in Europe

New calculations show that the ring of light surrounding a black hole is actually made up of infinite subrings that can’t be seen with current technology

The severity of this year's bleaching is second only to 2016, during which a third of the reef’s corals died

With many zoos and aquariums closed to the public, keepers let animals roam empty hallways to meet their neighbors

The purplish-blue pigment, derived from a Portuguese fruit, fell out of use by the 19th century

The snazzy search is part of the telescope’s 30th anniversary celebration

A rare bloom of microscopic organisms capable of making their own blue light has transformed several of the state’s beaches

Root vegetables like rutabagas and Jerusalem artichokes were ration staples during the Nazi occupation of Paris

The freedom fighter may have once used the structure to conspire against English forces

Participants promote a family-oriented fellowship of friends who like to beat each other up while wearing skates

Remote Isle au Haut is integrating time-tested technology with emerging innovations to create its own microgrid

View these vivid illustrations by Japanese artist Kōkichi Tsunoi of the varieties of trees presented to the United States in 1912

Tulips, cherry blossoms and orchids supply a ray of hope during self-isolation

Depicts a woman standing at the end of a staircase on the edge of a building, while the pipes coming from the structure continue on only to come to an end. The woman posing in the photo stares straight into the lens of the camera to emphasize the dividing nature of the image.

Yamagata February, the winter was approaching the end in the flatland. I was aiming for the summit of "Mt.zao" from the night. There is a beautiful crater lake which is still active as a volcano, called "Goshikinuma", and in the summer it is a beautiful lake of emerald green. It was covered with strong wind and fog, but it showed its appearance in a momentary fine weather. The crater lake where the accumulated snow began to melt was like a blue moon.

The Blue Insularis is a venomous pit viper species commonly found in Indonesia. This is a photo of a specimen feeding on a frog, and with another snake close by trying to take a bite.

A sled in the Smithsonian collections marks the historic race

History remembers the English queen as a murderous monster, but the real story of Mary I is far more nuanced

In the hours after disaster struck Anchorage, an unexpected figure named Genie Chance came to the rescue

During his 20-year professional career, his boisterous style endeared him to fans but rankled traditionalists

Fun things 300bluebottles sees at Youtube

Many retailers are eager to hear more details from the province about when and how they should reopen after weeks of being closed to the public. The next phase of recovery is expected to start Friday.

Two men are charged in connection with an illegal drug distribution operation after Winnipeg police seized more than $2.2 million worth of methamphetamine, cocaine and ecstasy.

The premier’s office confirmed the news in an email statement, blaming visibility issues under "very specific lighting conditions."

A large destination resort in Minnesota, just a few hours south of Thunder Bay, Ont., has some big expansion plans.

Retail stores in Fort William First Nation (FWFN) reopened to the general public on May 5, but they are under new operating requirements, the band council announced Friday. However, the residential area of the First Nation remains accessible only to people who live in the community.

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<span class="topsy_trackback_comment"><span class="topsy_twitter_username"><span class="topsy_trackback_content">Fresh trouble for SRK brought by the Shiv Sena men http://bit.ly/i8tocu #IMCRadio.net</span></span>

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Newfoundland and Labrador's already fragile economy suffered another gut-punch in April, with Statistics Canada reporting Friday that 29,000 jobs were lost in April alone.

Staff halted $100 service over pandemic concerns, forcing homeowners to private plumbers much more, and Danny Breen is urging them to contact the city.

Take a listen to Episode 9 of CBC Edmonton's new podcast, The Loop.

The NDP health critic says comments Premier Doug Ford made this week calling out medical officers of health for not getting enough COVID-19 testing done are uncalled for.

The head of a Sudbury-based book publishing company says she’s shifting launches online due to COVID-19.

Every year Washingtonians are treated to a feast for the eyes as ornamental cherry trees bloom across the city, most prominently by the Tidal Basin. As cherry blossom season approaches, we would like to share information about two related resources that we hope will offer some inspiration for those near and far: a selected set […]

For most immunoblots developed with chemiluminescence or with fluorochrome-based detection systems, it is possible to remove the primary and secondary antibodies from the membrane without affecting the bound antigen. This allows you to reuse the membrane for detection of another protein antigen. The blots developed with chromogenic substrates can also be stripped of antibodies and reprobed, but the bands detected in the first round of immunoblotting will remain unaffected. Stripping and reprobing of the membrane are particularly useful when the amount of sample is limited or when it is important to accurately compare the signal between two different protein antigens in the same sample. Examples of such experiments include determining the levels of a protein antigen in a series of samples relative to the loading control and comparison of the phosphorylated form to the total levels of the protein in the sample.

Before probing blots for the presence of an antigen, the total composition of the transferred proteins can be determined by staining the nitrocellulose or polyvinylidene fluoride (PVDF) membrane. Staining for proteins is useful to determine the position of the non-prestained molecular weight markers or individual lanes on the gel and to ensure that efficient transfer has occurred. It can be also used to verify equal loading of the samples in the gel when a comparison of the protein of interest between the different samples is important. The conventional procedures such as Coomassie Blue and silver staining methods used for staining polyacrylamide gels are incompatible with immunoblotting. Ponceau S is the more common staining method in immunoblotting protocols because it is compatible with antibody–antigen binding, is cost efficient, and provides a good contrast between the stained bands and background. In this protocol, nitrocellulose or PVDF membrane is rinsed with ultrapure H₂O after the transfer of proteins. Ponceau S dye is applied as an acidic aqueous solution, and the proteins on the membrane are stained with red color. The membrane is briefly destained with water and can be photographed or scanned to obtain the image of the total protein staining. Individual lane positions or the molecular weight standards can be marked with a pencil, if required.