Jay Collins is Vice Chairman Banking, Capital Markets and Advisory, Citigroup*

The post Only Sustainable Investment & Global Cooperation Can Counter COVID’s Blow to SDGs appeared first on Inter Press Service.

Denmark is Education Cannot Wait’s (ECW) third largest donor, with US$79.1 million in contributions to date. In this insightful interview with Denmark’s Minister for Development Cooperation, Rasmus Prehn, we explore the importance of girls’ education and gender equality, the humanitarian-development nexus, expanded engagement with the private sector, education in emergency response to the COVID-19 pandemic […]

The post The Power of Education in Emergencies: Interview with Denmark’s Minister of Development Cooperation Rasmus Prehn appeared first on Inter Press Service.

Normally they make tutus and elaborate costumes for some of the greatest opera and ballet stars in the world.     

Most candidates won’t work, but it’s essential to be ready to mass-produce the one that does.

[Dalsan Radio] US military operations in four countries in 2019 left 132 civilians dead, according to a report released Wednesday by the Pentagon.

NOWSHERA: The Prime Minister’s Corona Relief Tiger Force was made operational in the Nowshera district on Friday like other parts of the country.A total of 6,670 members of the force have been registered in the district who would work under the administration and other government...

The Philippines’ top broadcaster ABS-CBN on Tuesday was ordered off the air over a stalled operating licence renewal, drawing fresh charges that authorities were cracking down on press freedom.Since running afoul of President Rodrigo Duterte in 2016, the media powerhouse has seen bills to extend its franchise languish in Congress as the leader repeatedly attacked ABS-CBN in speeches.Duterte is notorious for tangling with media outlets critical of his policies, sparking concern that press…

Manssour Bin Mussallam, is Secretary General-elect of the Organisation of Educational Cooperation (OEC)

The post The COVID-19 Pandemic and the Reinvention of the Spirit of Solidarity and Cooperation appeared first on Inter Press Service.

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NOWSHERA: The Prime Minister’s Corona Relief Tiger Force was made operational in the Nowshera district on Friday like other parts of the country.A total of 6,670 members of the force have been...

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The historic spirit of cross-border trade in Central Asia is being revived in the energy sector.

Microchip Solves Interoperability Challenges of Delivering up to 90 Watts of Power Over Ethernet Wiring

Microchip Smart Storage Adapters Now Interoperate Seamlessly with MegaRAC® SP-X Management Firmware from AMI for At-Scale Secure Storage Management

Loan agreements outline the terms of an agreement for a loan. This document dated 6 May 2020 is provided for the ADB project 54183-001 in Bhutan.

ADB has a vacancy for the position of Senior Operations Assistant in the Pacific Department. The deadline for submitting applications is on 21 May 2020.

ADB has a vacancy for the position of Senior Operations Assistant in the Central and West Asia Department. The deadline for submitting applications is on 22 May 2020.

ADB President Masatsugu Asakawa and AFD Chief Executive Officer Rémy Rioux held discussions today on strengthening the partnership between ADB and AFD to respond to the novel coronavirus disease (COVID-19) pandemic.

ONGOING EVALUATION. : This evaluation will assess the performance of the Regional Cooperation and Integration Fund (RCIF) against its establishment objectives and provide recommendations for its future directions.

ADB has a vacancy for the position of Senior Operations Assistant in the Pacific Department. The deadline for submitting applications is on 25 May 2020.

ADB has a vacancy for the position of Senior Regional Cooperation Specialist in the Sustainable Development and Climate Change Department. The deadline for submitting applications is on 18 May 2020.

ADB has a vacancy for the position of Senior Operations Assistant in the South Asia Department. The deadline for submitting applications is on 19 May 2020.

ADB has a vacancy for the position of Senior Operations Assistant in the Pacific Department. The deadline for submitting applications is on 21 May 2020.

ADB has a vacancy for the position of Senior Operations Assistant in the Central and West Asia Department. The deadline for submitting applications is on 22 May 2020.

This document outlines the operations strategy and plan for the ASEAN Catalytic Green Finance Facility pilot phase (2019–2021).

A peer to peer discussion on how ADB works with the private sector to fill the investment gaps and finance facilities that generate and distribute clean energy.

ADB’s private sector operations aim to achieve development impact while ensuring profitability and commercial success for clients.

Energy security is a nation’s ability to meet the energy needs of its inhabitants uninterruptedly at an affordable price.

Trade facilitation, by reducing trade costs and raising the efficiency of moving goods across borders, is integral to international trade.

The global financial architecture should focus on providing short-term lending facilities to improve the efficiency of developing projects.

Energy security is a nation’s ability to meet the energy needs of its inhabitants uninterruptedly at an affordable price.

AN3265 - UPD301A USB Power Delivery Operation

STAMFORD, CT – HARMAN (NYSE:HAR), the premier connected technologies company for automotive, consumer and enterprise markets, today announced that Pradeep Chaudhry has been appointed Country Manager for its India operations, effective January 1, 2016....

During the early 1900s, the city of Mumbai witnessed an unprecedented peak in social, artistic, and cultural activity. At the center of this movement was the Royal Opera House, Mumbai, India’s ‘cultural crown jewel’ and only surviving opera house. Built...

Welsh mezzo-soprano Katherine Jenkins will stream a concert from an empty Royal Albert Hall on Friday evening, as locked-down Britain marks the 75th anniversary of "Victory in Europe" Day.

A rash of recent earthquakes in Lancashire, UK has prompted fracking operations to halt temporarily on six separate occasions

Opera singer Victoria Robertson is accustomed to performing on stages much bigger than the five-foot wide front porch of her San Diego home. But with concert venues closed and work at a standstill due to the coronavirus pandemic, she decided to make the most of it.

David Rhodes, the former president of CBS News who started his career at Fox News, is returning to Rupert Murdoch's News Corp to help the company's News UK operations in the video business, a source familiar with the matter said.

Title: Breast Reconstruction Often Involves Multiple Operations
Category: Health News
Created: 4/30/2015 12:00:00 AM
Last Editorial Review: 5/1/2015 12:00:00 AM

ABSTRACT

The global stress response controlled by the alternative sigma factor RpoS protects enteric bacteria from a variety of environmental stressors. The role of RpoS in other, nonenteric bacteria, such as the opportunistic pathogen Pseudomonas aeruginosa, is less well understood. Here, we employed experimental social evolution to reveal that cooperative behavior via secreted public goods is an important function in the RpoS response of P. aeruginosa. Using whole-genome sequencing, we identified rpoS loss-of-function mutants among isolates evolved in a protein growth medium that requires extracellular proteolysis. We found that rpoS mutants comprise up to 25% of the evolved population and that they behave as social cheaters, with low fitness in isolation but high fitness in mixed culture with the cooperating wild type. We conclude that rpoS mutants cheat because they exploit an RpoS-controlled public good produced by the wild type, the secreted aminopeptidase PaAP, and because they do not carry the metabolic costs of expressing PaAP and many other gene products in the large RpoS regulon. Our results suggest that PaAP is an integral part of a proteolytic sequence in P. aeruginosa that permits the utilization of protein as a nutrient source. Our work broadens the scope of stress response functions in bacteria.

IMPORTANCE Bacterial stress responses are generally considered protective measures taken by individual cells. Enabled by an experimental evolution approach, we describe a contrasting property, collective nutrient acquisition, in the RpoS-dependent stress response of the opportunistic human pathogen P. aeruginosa. Specifically, we identify the secreted P. aeruginosa aminopeptidase (PaAP) as an essential RpoS-controlled function in extracellular proteolysis. As a secreted "public good," PaAP permits cheating by rpoS mutants that save the metabolic costs of expressing RpoS-controlled genes dispensable under the given growth conditions. Proteolytic enzymes are important virulence factors in P. aeruginosa pathogenesis and constitute a potential target for antimicrobial therapy. More broadly, our work contributes to recent findings in higher organisms that stress affects not only individual fitness and competitiveness but also cooperative behavior.

Nuclear import of viral genomes is an important step during the life cycle of adenoviruses (AdV), requiring soluble cellular factors as well as proteins of the nuclear pore complex (NPC). We addressed the role of the cytoplasmic nucleoporin Nup358 during adenoviral genome delivery by performing depletion/reconstitution experiments and time-resolved quantification of adenoviral genome import. Nup358-depleted cells displayed reduced efficiencies of nuclear import of adenoviral genomes, and the nuclear import receptor transportin 1 became rate limiting under these conditions. Furthermore, we identified a minimal N-terminal region of Nup358 that was sufficient to compensate for the import defect. Our data support a model where Nup358 functions as an assembly platform that promotes the formation of transport complexes, allowing AdV to exploit a physiological protein import pathway for accelerated transport of its DNA.

IMPORTANCE Nuclear import of viral genomes is an essential step to initiate productive infection for several nuclear replicating DNA viruses. On the other hand, DNA is not a physiological nuclear import substrate; consequently, viruses have to exploit existing physiological transport routes. Here, we show that adenoviruses use the nucleoporin Nup358 to increase the efficiency of adenoviral genome import. In its absence, genome import efficiency is reduced and the transport receptor transportin 1 becomes rate limiting. We show that the N-terminal half of Nup358 is sufficient to drive genome import and identify a transportin 1 binding region. In our model, adenovirus genome import exploits an existing protein import pathway and Nup358 serves as an assembly platform for transport complexes.

Glycine riboswitches utilize both single- and tandem-aptamer architectures. In the tandem system, the relative contribution of each aptamer toward gene regulation is not well understood. To dissect these contributions, the effects of 684 single mutants of a tandem ON switch from Bacillus subtilis were characterized for the wild-type construct and binding site mutations that selectively restrict ligand binding to either the first or second aptamer. Despite the structural symmetry of tandem aptamers, the response to these mutations was frequently asymmetrical. Mutations in the first aptamer often significantly weakened the K_1/2, while several mutations in the second aptamer improved the amplitude. These results demonstrate that this ON switch favors ligand binding to the first aptamer. This is in contrast to the tandem OFF switch variant from Vibrio cholerae, which was previously shown to have preferential binding to its second aptamer. A bioinformatic analysis of tandem glycine riboswitches revealed that the two binding pockets are differentially conserved between ON and OFF switches. Altogether, this indicates that tandem ON switch variants preferentially utilize binding to the first aptamer to promote helical switching, while OFF switch variants favor binding to the second aptamer. The data set also revealed a cooperative glycine response when both binding pockets were maximally stabilized with three GC base pairs. This indicates a cooperative response may sometimes be obfuscated by a difference in the affinities of the two aptamers. This conditional cooperativity provides an additional layer of tunability to tandem glycine riboswitches that adds to their versatility as genetic switches.

BACKGROUND:Pneumoperitoneum and Trendelenburg position affect respiratory system mechanics and oxygenation during elective pelvic robotic surgery. The primary aim of this randomized pilot study was to compare the effects of a conventional low tidal volume ventilation with PEEP guided by gas exchange (VGas-guided) versus low tidal volume ventilation tailoring PEEP according to esophageal pressure (VPes-guided) on oxygenation and respiratory mechanics during elective pelvic robotic surgery.METHODS:This study was conducted in a single-center tertiary hospital between September 2017 and January 2019. Forty-nine adult patients scheduled for elective pelvic robotic surgery were screened; 28 subjects completed the full analysis. Exclusion criteria were American Society of Anesthesiologists physical status ≥ 3, contraindications to nasogastric catheter placement, and pregnancy. After dedicated naso/orogastric catheter insertion, subjects were randomly assigned to VGas-guided (FIO2 and PEEP set to achieve SpO2 > 94%) or VPes-guided (PEEP tailored to equalize end-expiratory transpulmonary pressure). Oxygenation (PaO2/FIO2) was evaluated (1) at randomization, after pneumoperitoneum and Trendelenburg application; (2) at 60 min; (3) at 120 min following randomization; and (4) at end of surgery. Respiratory mechanics were assessed during the duration of the study.RESULTS:Compared to VGas-guided, oxygenation was higher with VPes-guided at 60 min (388 ± 90 vs 308 ± 95 mm Hg, P = .02), at 120 min after randomization (400 ± 90 vs 308 ± 81 mm Hg, P = .008), and at the end of surgery (402 ± 95 vs 312 ± 95 mm Hg, P = .009). Respiratory system elastance was lower with VPes-guided compared to VGas-guided at 20 min (24.2 ± 7.3 vs 33.4 ± 10.7 cm H2O/L, P = .001) and 60 min (24.1 ± 5.4 vs 31.9 ± 8.5 cm H2O/L, P = .006) from randomization.CONCLUSIONS:Oxygenation and respiratory system mechanics were improved when applying a ventilatory strategy tailoring PEEP to equalize expiratory transpulmonary pressure in subjects undergoing pelvic robotic surgery compared to a VGas-guided approach. (ClinicalTrials.gov registration NCT03153592).

The quinoprotein glycine oxidase from the marine bacterium Pseudoalteromonas luteoviolacea (PlGoxA) uses a protein-derived cysteine tryptophylquinone (CTQ) cofactor to catalyze conversion of glycine to glyoxylate and ammonia. This homotetrameric enzyme exhibits strong cooperativity toward glycine binding. It is a good model for studying enzyme kinetics and cooperativity, specifically for being able to separate those aspects of protein function through directed mutagenesis. Variant proteins were generated with mutations in four active-site residues, Phe-316, His-583, Tyr-766, and His-767. Structures for glycine-soaked crystals were obtained for each. Different mutations had differential effects on kcat and K0.5 for catalysis, K0.5 for substrate binding, and the Hill coefficients describing the steady-state kinetics or substrate binding. Phe-316 and Tyr-766 variants retained catalytic activity, albeit with altered kinetics and cooperativity. Substitutions of His-583 revealed that it is essential for glycine binding, and the structure of H583C PlGoxA had no active-site glycine present in glycine-soaked crystals. The structure of H767A PlGoxA revealed a previously undetected reaction intermediate, a carbinolamine product-reduced CTQ adduct, and exhibited only negligible activity. The results of these experiments, as well as those with the native enzyme and previous variants, enabled construction of a detailed mechanism for the reductive half-reaction of glycine oxidation. This proposed mechanism includes three discrete reaction intermediates that are covalently bound to CTQ during the reaction, two of which have now been structurally characterized by X-ray crystallography.

PURPOSE

Operational failures are system-level errors in the supply of information, equipment, and materials to health care personnel. We aimed to review and synthesize the research literature to determine how operational failures in primary care affect the work of primary care physicians.

Store-operated calcium (Ca²⁺) entry (SOCE) occurs through a widely distributed family of ion channels activated by the loss of Ca²⁺ from the endoplasmic reticulum (ER). The best understood of these is the Ca²⁺ release-activated Ca²⁺ (CRAC) channel, which is notable for its unique activation mechanism as well as its many essential physiological functions and the diverse pathologies that result from dysregulation. In response to ER Ca²⁺ depletion, CRAC channels are formed through a diffusion trap mechanism at ER–plasma membrane (PM) junctions, where the ER Ca²⁺-sensing stromal interaction molecule (STIM) proteins bind and activate hexamers of Orai pore-forming proteins to trigger Ca²⁺ entry. Cell biological studies are clarifying the architecture of ER–PM junctions, their roles in Ca²⁺ and lipid transport, and functional interactions with cytoskeletal proteins. Molecular structures of STIM and Orai have inspired a multitude of mutagenesis and electrophysiological studies that reveal potential mechanisms for how STIM is toggled between inactive and active states, how it binds and activates Orai, and the importance of STIM-binding stoichiometry for opening the channel and establishing its signature characteristics of extremely high Ca²⁺ selectivity and low Ca²⁺ conductance.

Researchers investigated pain perception in patients with diabetic foot ulcers (DFUs) by analyzing pre- and postoperative physical function (PF), pain interference (PI), and depression domains of the Patient-Reported Outcome Measurement Information System (PROMIS). They hypothesized that 1) because of painful diabetic peripheral neuropathy (DPN), a majority of patients with DFUs would have high PROMIS PI scores unchanged by operative intervention, and 2) the initially assessed PI, PF, and depression levels would be correlated with final outcomes. Seventy-five percent of patients with DFUs reported pain, most likely because of painful DPN. Those who reported high PI and low PF were likely to report depression. PF, PI, and depression levels were unchanged after operative intervention or healing of DFUs.