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Direct transesterification of all classes of lipids in a one-step reaction

G Lepage
Jan 1, 1986; 27:114-120
Articles




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The plasma lecithin:cholesterol acyltransferase reaction

John A. Glomset
Mar 1, 1968; 9:155-167
Reviews




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Dopamine transporter trafficking and Rit2 GTPase: Mechanism of action and in vivo impact [Neurobiology]

Following its evoked release, dopamine (DA) signaling is rapidly terminated by presynaptic reuptake, mediated by the cocaine-sensitive DA transporter (DAT). DAT surface availability is dynamically regulated by endocytic trafficking, and direct protein kinase C (PKC) activation acutely diminishes DAT surface expression by accelerating DAT internalization. Previous cell line studies demonstrated that PKC-stimulated DAT endocytosis requires both Ack1 inactivation, which releases a DAT-specific endocytic brake, and the neuronal GTPase, Rit2, which binds DAT. However, it is unknown whether Rit2 is required for PKC-stimulated DAT endocytosis in DAergic terminals or whether there are region- and/or sex-dependent differences in PKC-stimulated DAT trafficking. Moreover, the mechanisms by which Rit2 controls PKC-stimulated DAT endocytosis are unknown. Here, we directly examined these important questions. Ex vivo studies revealed that PKC activation acutely decreased DAT surface expression selectively in ventral, but not dorsal, striatum. AAV-mediated, conditional Rit2 knockdown in DAergic neurons impacted baseline DAT surface:intracellular distribution in DAergic terminals from female ventral, but not dorsal, striatum. Further, Rit2 was required for PKC-stimulated DAT internalization in both male and female ventral striatum. FRET and surface pulldown studies in cell lines revealed that PKC activation drives DAT-Rit2 surface dissociation and that the DAT N terminus is required for both PKC-mediated DAT-Rit2 dissociation and DAT internalization. Finally, we found that Rit2 and Ack1 independently converge on DAT to facilitate PKC-stimulated DAT endocytosis. Together, our data provide greater insight into mechanisms that mediate PKC-regulated DAT internalization and reveal unexpected region-specific differences in PKC-stimulated DAT trafficking in bona fide DAergic terminals.




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The Challenge of Ambition? Unlocking Climate Action and the Outcomes of COP24




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Climate Action: A Role for Civil Disobedience?




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Undercurrents: Episode 48 - UK Intelligence Agencies, and Paying for Climate Action




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Atomic force microscopy-based characterization of the interaction of PriA helicase with stalled DNA replication forks [DNA and Chromosomes]

In bacteria, the restart of stalled DNA replication forks requires the DNA helicase PriA. PriA can recognize and remodel abandoned DNA replication forks, unwind DNA in the 3'-to-5' direction, and facilitate the loading of the helicase DnaB onto the DNA to restart replication. Single-stranded DNA–binding protein (SSB) is typically present at the abandoned forks, but it is unclear how SSB and PriA interact, although it has been shown that the two proteins interact both physically and functionally. Here, we used atomic force microscopy to visualize the interaction of PriA with DNA substrates with or without SSB. These experiments were done in the absence of ATP to delineate the substrate recognition pattern of PriA before its ATP-catalyzed DNA-unwinding reaction. These analyses revealed that in the absence of SSB, PriA binds preferentially to a fork substrate with a gap in the leading strand. Such a preference has not been observed for 5'- and 3'-tailed duplexes, suggesting that it is the fork structure that plays an essential role in PriA's selection of DNA substrates. Furthermore, we found that in the absence of SSB, PriA binds exclusively to the fork regions of the DNA substrates. In contrast, fork-bound SSB loads PriA onto the duplex DNA arms of forks, suggesting a remodeling of PriA by SSB. We also demonstrate that the remodeling of PriA requires a functional C-terminal domain of SSB. In summary, our atomic force microscopy analyses reveal key details in the interactions between PriA and stalled DNA replication forks with or without SSB.




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Climate Action in 2020: Time to Focus on Forests

14 January 2020

Alison Hoare

Senior Research Fellow, Energy, Environment and Resources Programme
More ambitious policies to reduce deforestation are key to effective climate policy, but to succeed, they require three big changes in approach.

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Mahogany tree seedlings being taken to be planted out in the Amazon. Photo: Getty Images.

December’s UN climate talks held in Madrid were aptly titled ‘Time for Action’. While little progress was made at the conference in establishing an international framework that would help to instigate this, there is still much scope for action in 2020. The need for this has become all too apparent as the impacts of climate change are increasingly seen around the world.

One of the key areas where progress can be made in 2020 is in increasing the ambition of nationally determined contributions (NDCs), these being governments’ plans to take action in response to climate change. To date, 184 countries have submitted NDCs, yet the commitments that have been made fall far short of what is needed to avert catastrophic climate change.

In 2020, however, many countries will be revising their NDCs, presenting an important opportunity to shift momentum; to date 79 countries have announced that they will be enhancing the ambition of their NDCs.

The forest sector is one area where more ambitious targets are likely to be set, and indeed, at the Climate Action Summit in September 2019, more than 20 countries made new commitments for the conservation, reforestation and restoration of their forests.

This will be essential. As is well documented, reducing deforestation is critical to reducing carbon emissions, while healthy and diverse forests are vital for adapting and increasing resilience to climate change.

However, while it is important that ambitious targets are set, this is relatively easy; the bigger challenge lies in ensuring that these are achievable.

This is all too apparent from experience thus far. In 2014, the New York Declaration on Forests set the goal to halve forest loss by 2020, and to end it by 2030. In addition, it included the goal to restore 150 million hectares of degraded landscapes and forestlands by 2020, and a further 200 million hectares by 2030.

The declaration has been endorsed by over 50 countries, as well as business and civil society organizations, yet the 2020 goals are far from being reached – in the six years since the declaration was launched, it has been found that forest loss increased rather than declined, and only about 27 million hectares of land have been restored.

What then is needed to ensure that the commitments being made by governments in their NDCs will actually be met? Three big changes are required.

Firstly, a shift in perspective is needed in many countries to a more forest-sensitive approach to development, one that gives adequate recognition to the full range of values provided by forests, rather than primarily focusing on their role as a global carbon sink. These include their importance for local and national economies, for livelihoods and the well-being of forest-dependent peoples, and for biodiversity and the regulation of local climate and water systems.

The focus on nature-based solutions at the international level offers potential to support this shift. However, it is critical that these are not seen as ‘niche’ approaches, and that countries identify what nature-based solutions mean for them, and how forests and tree-rich landscapes can best be integrated into their development strategies.

Fundamental to achieving this will be further improvements in governance, and this is the second change that is required. Legal and institutional reforms are needed in many countries as well as significant investments in human and technical resources. This will enable processes to be strengthened, or put in place, so that equitable strategies can be developed and implemented – strategies that reflect a balance of the needs and priorities of the full range of stakeholders, including local and global, rural and urban, women and men, young and old.

Financing will of course be critical for this, and the least developed countries in particular will be hindered in the actions they can take without additional finance. This is the third area of change that is needed, and it is to be hoped that the international community will make better progress on this in 2020. Forest and land-use options are often described as a cost-effective means of tackling climate change, as is noted in the Santiago Call for Action on Forests for example.

This is not to say that these will be easy or cheap – as Chatham House has documented, experience of forest governance reform has shown that it takes significant funding and time to bring about deep-rooted change. However, the huge potential benefits that can result, for the citizens of forest-rich countries as well as for the planet, mean that forests and sustainable land-use are a good investment.




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Spectral analysis and representation of solutions of integro-differential equations with fractional exponential kernels

V. V. Vlasov and N. A. Rautian
Trans. Moscow Math. Soc. 80 (2020), 169-188.
Abstract, references and article information





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Blaming China Is a Dangerous Distraction

15 April 2020

Jim O'Neill

Chair, Chatham House
Chinese officials' initial effort to cover up the coronavirus outbreak was appallingly misguided. But anyone still focusing on China's failings instead of working toward a solution is essentially making the same mistake.

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Medical staff on their rounds at a quarantine zone in Wuhan, China. Photo by STR/AFP via Getty Images.

As the COVID-19 crisis roars on, so have debates about China’s role in it. Based on what is known, it is clear that some Chinese officials made a major error in late December and early January, when they tried to prevent disclosures of the coronavirus outbreak in Wuhan, even silencing healthcare workers who tried to sound the alarm.

China’s leaders will have to live with these mistakes, even if they succeed in resolving the crisis and adopting adequate measures to prevent a future outbreak. What is less clear is why other countries think it is in their interest to keep referring to China’s initial errors, rather than working toward solutions.

For many governments, naming and shaming China appears to be a ploy to divert attention from their own lack of preparedness. Equally concerning is the growing criticism of the World Health Organization (WHO), not least by Donald Trump who has attacked the organization - and threatens to withdraw US funding - for supposedly failing to hold the Chinese government to account.

Unhelpful and dangerous

At a time when the top global priority should be to organize a comprehensive coordinated response to the dual health and economic crises unleashed by the coronavirus, this blame game is not just unhelpful but dangerous.

Globally and at the country level, we all desperately need to do everything possible to accelerate the development of a safe and effective vaccine, while in the meantime stepping up collective efforts to deploy the diagnostic and therapeutic tools necessary to keep the health crisis under control.

Given there is no other global health organization with the capacity to confront the pandemic, the WHO will remain at the center of the response, whether certain political leaders like it or not.

Having dealt with the WHO to a modest degree during my time as chairman of the UK’s independent Review on Antimicrobial Resistance (AMR), I can say that it is similar to most large, bureaucratic international organizations.

Like the International Monetary Fund (IMF), the World Bank, and the United Nations, it is not especially dynamic or inclined to think outside the box. But rather than sniping at these organizations from the sidelines, we should be working to improve them.

In the current crisis, we all should be doing everything we can to help both the WHO and the IMF to play an effective, leading role in the global response. As I have argued before, the IMF should expand the scope of its annual Article IV assessments to include national public-health systems, given that these are critical determinants in a country’s ability to prevent or at least manage a crisis like the one we are now experiencing.

I have even raised this idea with IMF officials themselves, only to be told that such reporting falls outside their remit because they lack the relevant expertise. That answer was not good enough then, and it definitely isn’t good enough now.

If the IMF lacks the expertise to assess public health systems, it should acquire it. As the COVID-19 crisis makes abundantly clear, there is no useful distinction to be made between health and finance. The two policy domains are deeply interconnected, and should be treated as such.

In thinking about an international response to today’s health and economic emergency, the obvious analogy is the 2008 global financial crisis which started with an unsustainable US housing bubble, fed by foreign savings owing to the lack of domestic savings in the United States.

When the bubble finally burst, many other countries sustained more harm than the US did, just as the COVID-19 pandemic has hit some countries much harder than it hit China.

And yet not many countries around the world sought to single out the US for presiding over a massively destructive housing bubble, even though the scars from that previous crisis are still visible. On the contrary, many welcomed the US economy’s return to sustained growth in recent years, because a strong US economy benefits the rest of the world.

So, rather than applying a double standard and fixating on China’s undoubtedly large errors, we would do better to consider what China can teach us. Specifically, we should be focused on better understanding the technologies and diagnostic techniques that China used to keep its - apparent - death toll so low compared to other countries, and to restart parts of its economy within weeks of the height of the outbreak.

And for our own sakes, we also should be considering what policies China could adopt to put itself back on a path toward 6% annual growth, because the Chinese economy inevitably will play a significant role in the global recovery.

If China’s post-pandemic growth model makes good on its leaders’ efforts in recent years to boost domestic consumption and imports from the rest of the world, we will all be better off.

This article was originally published in Project Syndicate




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Online Forum on Strategic Approaches to Capacity-building in Biosafety and the Comprehensive Review of the Capacity-Building Action Plan (20 February - 4 May 2012)




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New publication: Framework and Action Plan for Capacity-Building for the Effective Implementation of the Cartagena Protocol on Biosafety




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CBD News: Press release; Governments open meeting in Bonn to take action on declining biodiversity resources.




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CBD News: Biodiversity added to the agenda of Hokkaido Toyako G8 Summit. G8 Environmental Ministers issue the "Kobe Call for Action for Biodiversity".




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CBD News: Parliamentarians Issue Declaration in Support of Biodiversity Action at Bonn Conference.




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CBD News: CBD provides free tool to identify win-win areas for REDD and biodiversity. The Bali Action plan under the United Nations FCCC encourages efforts to reduce emissions from reducing emissions from deforestation and forest degradation (REDD), while




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CBD News: Joint press-release by GEF and CBD on the approval by the GEF Council of a special initiative designed to build global awareness and catalyze decisive action during the upcoming 2010 International Year of Biodiversity (IYB).




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CBD Communiqué: The Group of 77 Initiates the Preparation of a Multi-Year Plan of Action for South-South Cooperation on Biodiversity for Development.




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CBD News: Statement to UNFCCC SBSTA 31: Agenda Item 5 Reducing Emissions from Deforestation in Developing Countries: Approaches to Stimulate Action.




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CBD News: Statement by Mr Ahmed Djoghlaf, Executive Secretary of the Convention on Biological Diversity, on the occasion of the XVI Consultative Group Meeting and the XVI General Authority Meeting of the Plan of Action for the Protection of the Marine Env




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CBD News: Statement by Mr. Ahmed Djoghlaf, Executive Secretary of the Convention on Biological Diversity, on the occasion of the Inaugural Meeting of the Local Action for Biodiversity (LAB) Advisory Committee, 24 March 2010, Rio De Janeiro, Brazil.




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CBD Press Release: The Arctic Biodiversity Assessment Another Wake Up Call for Action.




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CBD Communiqué: China expedites finalization of its new biodiversity strategy and action plan




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CBD Communiqué: Call for urgent action by the Aichi International Youth Conference on Biodiversity.




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CBD Communiqué: UN General Assembly Event to Spur Action to Stem Further Biodiversity Loss.




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CBD Communiqué: The Ministers of Foreign Affairs of the Group of 77 and China support the adoption of the Multi-Year Plan of Action on South-South Cooperation on Biodiversity for Development at the tenth meeting of the Conference of the Parties to th




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CBD News: Statement by Mr. Ahmed Djoghlaf, CBD Executive Secretary, on the occasion of the Pre-COP10-MOP5 Citizen Forum - Social Sustainability and Biological Safety Policy Reactions & Public Participation, Nagoya, Japan, 9 October 2010.




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CBD Press Release: The Group of 77 and China propose a Multi-Year Plan of Action for South-South Cooperation on Biodiversity for Development for adoption at the tenth meeting of the Conference of the Parties.




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CBD Joint Press Release: Mobilizing society to support biodiversity: As Countdown 2010 initiative closes, local coordinated actions show way to solving the global biodiversity crisis.




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CBD Press Release: From commitment to action: over US$ 120 million announced for biodiversity and protected areas.




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CBD Press Release: United Nations Goodwill Ambassador for Biodiversity Finds Inspiring Commitment and Action for the Environment in Visit to Rwanda.




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CBD Communiqué: Rwanda Launches a Revision of Its National Biodiversity Strategy and Action Plan.




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CBD Communiqué: Regional Workshop on updating National Biodiversity Strategies and Action Plans Being Held in Congo




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CBD Communiqué: Capacity-Building Workshop for the Pan-European Biological and Landscape Diversity Strategy Members on Updating National Biodiversity Strategy and Action Plans




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CBD Communiqué: Regional workshop for East Africa on updating national biodiversity strategies and action plans (NBSAPs) held in Kigali




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CBD Communiqué: Geneva Call for Urgent Action on the Implementation of the Successful Nagoya Outcomes




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CBD Communiqué: Regional workshop for Pacific on updating national biodiversity strategies and action plans celebration of the United Nations Decade on Biodiversity




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CBD News: Statement by Mr. Braulio Ferreira de Souza Dias, CBD Executive Secretary, on the occasion of Regional Workshop for Africa on Updating National Biodiversity Strategies and Action Plans, Addis Ababa, Ethiopia, 27 February, 2012




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CBD News: Statement by Mr. Braulio Ferreira de Souza Dias, CBD Executive Secretary, on the occasion of the Third Meeting on the Implementation of the Plan of Action on Sub-National Governments, Cities and Other Local Authorities for Biodiversity, Nagoya,




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CBD Press Release: Action in support of Biodiversity urgently needed: UN Meeting on Biodiversity opens in Hyderabad India




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CBD Press Release: From words to action - key organizations team up to stop the extinction crisis.




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CBD News: Statement by Mr. Braulio Ferreira de Souza Dias, CBD Executive Secretary, on the Occasion of the Global Workshop on Reviewing Progress and Building Capacity for the National Biodiversity Strategies and Action Plans Revision Process, Nairobi, Ken




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CBD News: On the International Day for Biological Diversity, celebrated under the theme of Island Biodiversity, islands are taking action to effectively conserve biodiversity and promote sustainable livelihoods. These actions, or "Bright Spots,"




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CBD News: Islands are taking action to effectively conserve biodiversity and promote sustainable livelihoods. Despite significant vulnerabilities facing islands, leaders of island countries and countries with islands have made visionary commitments at loc




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CBD News: To avoid landscape fragmentation and loss of species and habitats for biodiversity, participants to a three-day workshop in Kurupukari, Guyana, have agreed on a Regional Action Plan related to biological corridors, connectivity conservation and




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CBD News: Governments attending a United Nations meeting on the safe use of living modified organisms have agreed on various actions to advance the implementation of the Cartagena Protocol on Biosafety to the Convention on Biological Diversity




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CBD News: Bold and innovative action is urgently required if governments are to meet the globally-agreed Strategic Plan for Biodiversity and its Aichi Targets by 2020, says a United Nations progress report on the state of global biodiversity.




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CBD News: The Convention on Biological Diversity closed the 12th meeting of the Conference of the Parties today with Governments committing to step up actions to achieve, by the end of the decade, the Aichi Biodiversity Targets agreed four years ago, and




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CBD News: Governments have agreed to a series of actions that will further bolster the Nagoya Protocol on Access and Benefit Sharing, facilitate its effective implementation, and ensure that countries have the necessary resources to take the next steps to