Investments in new clean-energy capacity will total $1.61 trillion through 2020 even as the expansion of renewables is expected to slow, the International Energy Agency said.

President Barack Obama pledged deeper U.S. cuts in greenhouse-gas emissions and China will for the first time set a target for capping carbon emissions under an agreement between the world’s two biggest economies.

Warborough Investments Limited v Lunar Office SARL [2018] EWCA Civ 427 The Court of Appeal has held that, contrary to the decision at first instance, the sub-clauses of a lease setting out the terms for permitting underletting should be read cumula...

The 2017 edition of the Getting the Deal Through: Public Procurement is now live. The Polish chapter has been developed by Tomasz Zalewski, head of the public procurement team at Wierzbowski Eversheds Sutherland. The contributing editor of the Guide...

The 2018 edition of the Getting the Deal Through: Public Procurement is now live. The contributing editor of the Guide is Totis Kotsonis, competition lawyer and head of procurement at Eversheds Sutherland. The publication contains expert local insig...

Witold Slawinski, head of our tech & engineering team, contributed to the guide "Public Procurment 2019" - expert local insight into public procurement procedures in 34 jurisdictions, addressing such crucial topics as: legislative framework, app...

Eversheds Sutherland were delighted to partner with Women in ETFs for a one hour webinar 'Navigating Lockdown and Beyond - Practical steps to support ourselves and others through a unique time' on 30 April. The presentation was delivered by Á...

No support in the Corona crisis... The measures already initiated by the German Federal Government and the EU Commission to support and secure liquidity in the Corona crisis are focused on the industries directly affected by Corona/ COVID 19. They ...

PEBBLE BEACH: Gary Woodland took a two-shot lead over two-time defending champion Brooks Koepka and Justin Rose into the back nine at Pebble Beach on Sunday in the final round of the US Open Golf Championship.

WASHINGTON: Secretary of State Mike Pompeo vowed Sunday that the US will guarantee free passage through the vital Strait of Hormuz, as he accused Iran of recent attacks on oil tankers and the downing of a US drone.

On 17 May 2019, the European Council established a framework and implemented a new sanctions regime which now allows the EU to impose targeted sanctions to “deter and respond to cyber-attacks which constitute an external threat to the EU and i...

Nanjing University life science faculty's discovery of the anti-tumor mechanisms of high-salt diet was recently reported by Nature Communication and hailed by colleagues as a disruptive step of progress. Professors Zhang Junfeng and Dong Lei's team at the State Key Laboratory of Pharmaceutical Biotechnology of School of Life Sciences published in the online journal on April 7, 2020, the paper 'High-salt diet inhibits tumor growth in mice via regulating myeloid-derived suppressor cell differentiation' (https://doi.org/10.1038/s41467-020-15524-1). The publication drew widespread interest from at home and abroad. The Nature Research Cancer...

The following is intended to clarify how weight can be controlled when hunger is kept at bay using a reliable, informative tool called the glycemic index.

In the lead-up to the Truth Matters conference in October, we will be focusing our attention on the sufficiency, authority, and clarity of Scripture. Of our previous blog series, none better embodies that emphasis than Frequently Abused Verses. The following entry from that series originally appeared on February 19, 2016. -ed.

Tim Tebow was featured on the cover of the July 27, 2009 issue of Sports Illustrated, decked out in his Florida Gators uniform. But what made the image so striking was the message written in Tebow’s eye black—under his right eye was the word “Phil,” and under his left the numbers “4:13.”

That inscription may have been meaningless to the average football fanatic, but Tebow’s large evangelical constituency certainly recognized it as Bible reference. As he explained years later in an interview, he chose Philippians 4:13 because “‘There’s not a better verse for an athlete.’ It reads, ‘I can do all this [sic] through Him who gives me strength.’” ^[1] http://www.christianpost.com/news/tim-tebow-explains-why-he-tebows-uses-bible-verses-70824/#96Qczp0O7LZR8jsT.99

It’s not hard to understand the gravitational pull a verse like that could have on an athlete. No doubt countless men and women invoke God’s power for their various feats of strength and stamina. Even Jon Jones—a notorious MMA fighter who pummels people for a living—has it tattooed across his chest.

And in this era of unbridled self-esteem, who wouldn’t want the power of God enabling and animating the fulfilment of his hopes and dreams? Celebrity pastor, Joel Osteen, does nothing to quench such optimism and enthusiasm.

It is possible to see your dreams fulfilled. It is possible to overcome that obstacle. It is possible to climb to new heights. It is possible to embrace your destiny. You may not know how it will all take place. You may not have a plan, but all you have to know is that if God said you can . . . you can! Today, why don’t you begin to open yourself up to possibilities in your future by simply declaring this verse, “I can do all things through Christ who gives me strength?” ^[2] http://devotion.wedaretobelieve.com/2013/01/i-can-joel-osteen-ministries-daily.html

Osteen’s interpretation begs an important question about Philippians 4:13. When Paul wrote that he—and by extension, we—can “do all things” through Christ’s strength, was he promising victory and success in all our personal endeavors? Does “all things” essentially mean anything we want? And if so, why does any Christian ever fail at anything?

The preceding verses make Paul’s true intent quite clear:

Not that I speak from want, for I have learned to be content in whatever circumstances I am. I know how to get along with humble means, and I also know how to live in prosperity; in any and every circumstance I have learned the secret of being filled and going hungry, both of having abundance and suffering need. I can do all things through Him who strengthens me. (Philippians 4:11–13).

Christ’s strength wasn’t just a vague force enabling Paul to whatever ends he desired. It strengthened Paul to be content in spite of the harsh difficulties he faced. He wasn’t talking about hypothetical goals, but about the very real adversity he faced on a daily basis.

Specifically, he was talking about his unfair imprisonment at the time of his writing to the Philippian believers. Here’s how he described it at the beginning of his epistle:

Now I want you to know, brethren, that my circumstances have turned out for the greater progress of the gospel, so that my imprisonment in the cause of Christ has become well known throughout the whole praetorian guard and to everyone else, and that most of the brethren, trusting in the Lord because of my imprisonment, have far more courage to speak the word of God without fear. (Philippians 1:12–14) 

Paul wrote that he could do all things through Christ strengthening him when he was confined to the squalor and oppression of a Roman prison cell. And through his divine strengthening, He was able to look beyond his own suffering and rejoice in the gospel’s furtherance as a result of his imprisonment.

Paul never “discovered the champion” in himself, nor did he long for the fulfillment of his personal dreams. His delight was in extending the reach and influence of the gospel, and he labored to that end whether he was free or incarcerated. He was the benchmark of suffering for the sake of the gospel (2 Corinthians 11:23–33), and he rejoiced in the strength Christ gave him to endure all of it. John MacArthur elaborates:

No matter how difficult his struggles may have been, Paul had a spiritual undergirding, an invisible means of support. His adequacy and sufficiency came from his union with the adequate and sufficient Christ: “I have been crucified with Christ; and it is no longer I who live, but Christ lives in me; and the life which I now live in the flesh I live by faith in the Son of God, who loved me and gave Himself up for me” (Galatians 2:20). . . . Paul was strong enough to endure anything through Him who strengthen[ed] him. . . . What he is saying is that when he reached the limit of his resources and strength, even to the point of death, he was infused with the strength of Christ. He could overcome the most dire physical difficulties because of the inner, spiritual strength God had given him. ^[3] John MacArthur, The MacArthur New Testament Commentary: Philippians (Chicago: Moody Press, 2001) 303.

Philippians 4:13 doesn’t lose any relevance just because we’re not allowed to define “all things” as everything we want to do. On the contrary, Paul’s example of suffering has the broadest possible application for Christians: “All who desire to live godly in Christ Jesus will suffer persecution” (2 Timothy 3:12 NKJV, emphasis added). Suffering shouldn’t come as a surprise to the Christian. Whether or not we end up in a prison cell like Paul, we can embrace Philippians 4:13 as he did—the promise of Christ’s strength to endure all suffering for His sake.

Prague Daily Monitor

Strong winds felled trees on to cars and flung loose materials across the city on Tuesday afternoon. The spokesperson for the Prague Firefighter Brigade said that "so far we have responded to 23 incidents related to wind up to 15:00. The most common calls were related to fallen trees, scattered branches and debris."

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Cathay Pacific is looking at “structural change” as it investigates how to downscale its business in the wake of the coronavirus pandemic, the Post has learned.Hong Kong’s flag carrier is mulling scenarios that could reduce staff headcount, routes served and planes flown, as well as the possible consolidation of its airline brands, in drastic steps that would mirror those taken by rivals in recent weeks.“We are currently working with colleagues from across the airline to model varying degrees…

ISLAMABAD: Adviser to the Prime Minister on Finance and Revenue Dr Abdul Hafeez Shaikh has said the government is actively resolving the liquidity related issues of the business community by clearing...

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ISLAMABAD: Adviser to the Prime Minister on Finance and Revenue Dr Abdul Hafeez Shaikh has said the government is actively resolving the liquidity related issues of the business community by clearing...

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ISLAMABAD: Former Chairman Senate Mian Raza Rabbani Friday questioned the formation of the National Council for Minorities through notification and termed it violation of the judgement of the...

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ISLAMABAD: Adviser to the Prime Minister on Finance and Revenue Dr Abdul Hafeez Shaikh has said the government is actively resolving the liquidity related issues of the business community by clearing...

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RAWALPINDI: The countrywide protests by the Geo-Jang Group workers against the deteriorating media environment, disrupting circulation of dailies Jang and The News and incarcerating Editor-in-Chief Mir Shakil-ur-Rahman on fabricated charges continued on Friday.The countrywide protests by the...

radeep Sharma Tribune News Service Chandigarh, January 23 The group-housing societies, residential colonies of employers and commercial-cum-residential complexes of developers will be supplied power on single-point (bulk supply) system by the electricity department from April 1. While the electricity bills for February/March would be issued as per the existing practice, the bills from April onwards would be issued as per the single-point metering system, according to an order issued by the Chief General Manager (Commercial), Uttar Haryana Bijli Vitran Nigam (UHBVN). The new system would be implemented in housing colonies having 20 or more houses. The order said on the basis of the reading of April 1, the bills of […]

The Strengthening Technical and Vocational Education and Training Project took on some of the obstacles to creating a place for women in the so-called blue-collar occupations.

ADB’s private sector operations aim to achieve development impact while ensuring profitability and commercial success for clients.

STAMFORD, CT – HARMAN, the award-winning maker of outstanding audio and entertainment solutions, announced today that eight new products have been named CES Innovation Award winners for 2014. The awards were presented by the Consumer Electronics Association (CEA®), producer of the International Consumer Electronics Show (CES), the world’s largest consumer technology tradeshow. Products from HARMAN’s JBL, Harman Kardon, Infinity, AKG, and Aha brands were recognized for outstanding design and engineering in consumer electronics.

BENGALURU, INDIA –  HARMAN (NYSE:HAR), the premier connected technologies company for automotive, consumer and enterprise markets, today announced that it has launched its global corporate social responsibility initiative, ‘HARMAN Inspired’, in India....

By Dr. Cristina Bettencourt, Head of HARMAN University   At HARMAN, we owe every degree of our success to our talented and dedicated employees. While we already have extraordinary talent across all of our operations, we never stop trying to improve by...

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ABSTRACT

Horizontal gene transfer (HGT) promotes the spread of genes within bacterial communities. Among the HGT mechanisms, natural transformation stands out as being encoded by the bacterial core genome. Natural transformation is often viewed as a way to acquire new genes and to generate genetic mixing within bacterial populations. Another recently proposed function is the curing of bacterial genomes of their infectious parasitic mobile genetic elements (MGEs). Here, we propose that these seemingly opposing theoretical points of view can be unified. Although costly for bacterial cells, MGEs can carry functions that are at points in time beneficial to bacteria under stressful conditions (e.g., antibiotic resistance genes). Using computational modeling, we show that, in stochastic environments, an intermediate transformation rate maximizes bacterial fitness by allowing the reversible integration of MGEs carrying resistance genes, although these MGEs are costly for host cell replication. Based on this dual function (MGE acquisition and removal), transformation would be a key mechanism for stabilizing the bacterial genome in the long term, and this would explain its striking conservation.

IMPORTANCE Natural transformation is the acquisition, controlled by bacteria, of extracellular DNA and is one of the most common mechanisms of horizontal gene transfer, promoting the spread of resistance genes. However, its evolutionary function remains elusive, and two main roles have been proposed: (i) the new gene acquisition and genetic mixing within bacterial populations and (ii) the removal of infectious parasitic mobile genetic elements (MGEs). While the first one promotes genetic diversification, the other one promotes the removal of foreign DNA and thus genome stability, making these two functions apparently antagonistic. Using a computational model, we show that intermediate transformation rates, commonly observed in bacteria, allow the acquisition then removal of MGEs. The transient acquisition of costly MGEs with resistance genes maximizes bacterial fitness in environments with stochastic stress exposure. Thus, transformation would ensure both a strong dynamic of the bacterial genome in the short term and its long-term stabilization.

ABSTRACT

During infection of human parvovirus B19 (B19V), one viral precursor mRNA (pre-mRNA) is transcribed by a single promoter and is alternatively spliced and alternatively polyadenylated. Here, we identified a novel cis-acting sequence (5'-GUA AAG CUA CGG GAC GGU-3'), intronic splicing enhancer 3 (ISE3), which lies 72 nucleotides upstream of the second splice acceptor (A2-2) site of the second intron that defines the exon of the mRNA encoding the 11-kDa viral nonstructural protein. RNA binding motif protein 45 (RBM45) specifically binds to ISE3 with high affinity (equilibrium dissociation constant [K_D] = 33 nM) mediated by its RNA recognition domain and 2-homo-oligomer assembly domain (RRM2-HOA). Knockdown of RBM45 expression or ectopic overexpression of RRM2-HOA in human erythroid progenitor cells (EPCs) expanded ex vivo significantly decreased the level of viral mRNA spliced at the A2-2 acceptor but not that of the mRNA spliced at A2-1 that encodes VP2. Moreover, silent mutations of ISE3 in an infectious DNA of B19V significantly reduced 11-kDa expression. Notably, RBM45 also specifically interacts in vitro with ISE2, which shares the octanucleotide (GGGACGGU) with ISE3. Taken together, our results suggest that RBM45, through binding to both ISE2 and ISE3, is an essential host factor for maturation of 11-kDa-encoding mRNA.

IMPORTANCE Human parvovirus B19 (B19V) is a human pathogen that causes severe hematological disorders in immunocompromised individuals. B19V infection has a remarkable tropism with respect to human erythroid progenitor cells (EPCs) in human bone marrow and fetal liver. During B19V infection, only one viral precursor mRNA (pre-mRNA) is transcribed by a single promoter of the viral genome and is alternatively spliced and alternatively polyadenylated, a process which plays a key role in expression of viral proteins. Our studies revealed that a cellular RNA binding protein, RBM45, binds to two intron splicing enhancers and is essential for the maturation of the small nonstructural protein 11-kDa-encoding mRNA. The 11-kDa protein plays an important role not only in B19V infection-induced apoptosis but also in viral DNA replication. Thus, the identification of the RBM45 protein and its cognate binding site in B19V pre-mRNA provides a novel target for antiviral development to combat B19V infection-caused severe hematological disorders.

ABSTRACT

Iron is a vital mineral for almost all living organisms and has a pivotal role in central metabolism. Despite its great abundance on earth, the accessibility for microorganisms is often limited, because poorly soluble ferric iron (Fe³⁺) is the predominant oxidation state in an aerobic environment. Hence, the reduction of Fe³⁺ is of essential importance to meet the cellular demand of ferrous iron (Fe²⁺) but might become detrimental as excessive amounts of intracellular Fe²⁺ tend to undergo the cytotoxic Fenton reaction in the presence of hydrogen peroxide. We demonstrate that the complex formation rate of Fe³⁺ and phenolic compounds like protocatechuic acid was increased by 46% in the presence of HCO₃^– and thus accelerated the subsequent redox reaction, yielding reduced Fe²⁺. Consequently, elevated CO₂/HCO₃^– levels increased the intracellular Fe²⁺ availability, which resulted in at least 50% higher biomass-specific fluorescence of a DtxR-based Corynebacterium glutamicum reporter strain, and stimulated growth. Since the increased Fe²⁺ availability was attributed to the interaction of HCO₃^– and chemical iron reduction, the abiotic effect postulated in this study is of general relevance in geochemical and biological environments.

IMPORTANCE In an oxygenic environment, poorly soluble Fe³⁺ must be reduced to meet the cellular Fe²⁺ demand. This study demonstrates that elevated CO₂/HCO₃^– levels accelerate chemical Fe³⁺ reduction through phenolic compounds, thus increasing intracellular Fe²⁺ availability. A number of biological environments are characterized by the presence of phenolic compounds and elevated HCO₃^– levels and include soil habitats and the human body. Fe²⁺ availability is of particular interest in the latter, as it controls the infectiousness of pathogens. Since the effect postulated here is abiotic, it generally affects the Fe²⁺ distribution in nature.