The discovery of worms and snails confirms that these still-mysterious, dark hotspots of life extend beyond what’s visible above the crust

Created in London in the 1960s, the tracks are heading to auction as part of a larger collection of memorabilia connected to the famous American guitarist

By analyzing an old lidar survey, researchers found evidence of more than 6,500 ancient structures in a previously unexplored area of Campeche

Thousands of years ago, our ancestors used symbols to track the sale of textile and agricultural products. New research suggests that these markings informed the development of writing

The bone, described two decades after its discovery, suggests the species might have grown up to 20 percent bigger than other terror birds

Lula the bear and Simba the lion have been evacuated to a wildlife shelter in Jordan

A Port Hawkesbury woman has long imagined a Remembrance Day with her great-uncle's war medals. It will happen this year for the first time thanks to some sleuthing and the kindness of her great-uncle's foster family.

Mosquitoes take blood meals from a diverse range of host animals and their host associations vary by species. Characterizing these associations is an important element of the transmission dynamics of mosquito-vectored pathogens. To characterize mosquito host associations, various molecular techniques have been developed, which are collectively referred to as blood meal analysis. DNA barcoding has diverse biological applications and is well-suited to mosquito blood meal analysis. The standard DNA barcoding marker for animals is a 5' fragment of the cytochrome c oxidase I (COI) gene. A major advantage of this marker is its taxonomic coverage in DNA sequence reference databases, making it feasible to identify a wider range of mosquito host species than with any other gene. However, the COI gene contains high sequence variation at potential priming sites between vertebrate orders. Coupled with the need for primer sequences to be mismatched with mosquito priming sites so that annealing to mosquito DNA is inhibited, it can be difficult to design primers suitable for blood meal analysis applications. Several primers are available that perform well in mosquito blood meal analysis, annealing to priming sites for most vertebrate host taxa, but not to those of mosquitoes. Because priming site sequence variation among vertebrate taxa can cause amplification to fail, a hierarchical approach to DNA barcoding-based blood meal analysis can be applied. In such an approach, no single primer set is expected to be effective for 100% of potential host species. If amplification fails in the initial reaction, a subsequent reaction is attempted with primers that anneal to different priming sites, and so on, until amplification is successful.

Mosquito species vary in their host associations. Although some species are relative generalists, most specialize, to varying extents, on particular types of host animals. Mosquito host associations are among the most important factors that influence the transmission dynamics of mosquito-vectored pathogens, and understanding these associations can provide insight on how such pathogens move within ecosystems. Characterization of the host associations of mosquito species requires applying blood meal analysis to the largest possible sample size of mosquito blood meals. Processing large samples of mosquito blood meals can be time-consuming, especially when chain-termination sequencing is used, necessitating individual processing of each specimen. Various methods and commercially available kits and products are available for extracting DNA from mosquito blood meals. The hot sodium hydroxide and Tris (HotSHOT) method is a rapid and inexpensive method of DNA extraction that is compatible with the recovery of DNA from mosquito blood meals preserved on QIAcard Flinders Technology Associates (FTA) Classic Cards (FTA cards). FTA cards allow nucleic acids found in blood meals to be preserved easily, even in field conditions. DNA prepared using this method is suitable for polymerase chain reaction (PCR)-based blood meal analysis.

All PCR- and DNA-based blood meal analyses require host DNA from a mosquito blood meal to be effectively preserved between the time when the specimen is collected and the extraction of DNA. As soon as a mosquito ingests blood from a host animal, digestion of host cells and cellular components within the blood meal by enzymes in the mosquito midgut begins to degrade the host DNA templates that are the targets of polymerase chain reaction (PCR) amplification. Without effective preservation, host DNA is typically undetectable by PCR 48 h after feeding, because of digestion. Preservation methods for mosquito blood meals vary in their efficacy, and the logistics of fieldwork can limit the options for preservation of blood meals and maintenance of the integrity of host DNA. This protocol describes a method of blood meal preservation that is effective, convenient, and amenable to fieldwork in remote locations where cryopreservation at –20°C or –80°C may not be feasible. It uses a Flinders Technology Associates (FTA) card, which is a chemically treated card that lyses cells and allows nucleic acids to be preserved. This method is also expected to preserve the DNA or RNA of pathogens present within the engorged mosquito abdomen, including RNA viruses.

A federal audit of Canada’s strategy to promote the extraction of critical minerals says the government isn’t giving due consideration to the potential impact of mining on the environment, biodiversity and Indigenous people.

This will be the first Remembrance Day celebrated at the new Royal Canadian Legion in Sault Ste. Marie. Branch 25 recently opened a new complex in the northern Ontario city, complete with a 108-unit apartment tower.

Team Reid Carruthers has started the search for a new second after Derek Samagalski's decision to step back from the Winnipeg-based rink.

Vanessa James and Eric Radford withdrew from the pairs competition at the Canadian figure skating championships before Saturday's free program in Ottawa.

CBC Sports' daily newsletter breaks down whose fortunes are looking up and whose are down after the weekend's winter Olympic sports action.

The United States took the lead in the pairs and ice dance on the first day of the Four Continents figure skating championships on Thursday, and Japan's Mai Mihara led the women's event.

Bob Iaccino, Path Trading Partners

The Ontario Liberal Party is pledging to reduce the income tax rate for middle class families and eliminate provincial sales tax on hydro bills, if they win in the 2026 election.

After COVID-19 challenges forced the cancellation of Canada's mixed doubles trials, several top curlers are anxiously waiting to learn whether they will be chosen to represent the country in the discipline at the Beijing Games.

University

Power of One project's goal is perfecting solar technology for tomorrow's hybrid cars as high oil prices peak interest in alternative vehicles

MatWeb's new export engine pipes data from its 47,000 material information sheetsdirectly into SolidWorks and COSMOSWorks

Stress, fluid flow, and motion analysis lend credence to company's designs for Boeing, Airbus, and other major aerospace companies

Find out the latest news and updates on the CME Group Metals market, including the continued growth we are seeing with our Precious Metals products.

Get all the latest updates on COMEX Copper options including a new all-time high trading record and the strong growth of RFQs.

Hospitality stakeholders and organizations can serve a significant role in the integration of people who have criminal records or histories of incarceration back into the workforce, according to a new framework created by researchers in the Penn State School of Hospitality Management.

Early Black Friday deals are in effect on Apple's 2024 MacBook Pro M4, with every configuration — retail and CTO — eligible for a coupon discount.

Evidence suggests that the earlier Chinese Salt Typhoon breach of American telecoms may have led to the potential hacking of two presidential campaign officials' iPhones.

You can score up to $150 off Apple's brand-new 2024 M4 iMac and get your hands on one of the best all-in-one desktops. Plus, AppleCare is discounted too.

Apple's 1TB M4 MacBook Pro 14-inch is eligible for a material early Black Friday discount at Amazon, along with savings on the 2024 Mac mini M4 and a record low price on the 13-inch MacBook Air 16GB.

A newly-discovered iPhone 14 Pro prototype features one of the earliest known incarnations of Apple's scrapped haptic button project.

Rex from the USA, volunteered for two months in the Baseball and Softball Programme alongside OMers Terry Lingenhoel and Suzanne Cole this spring.

Larry Shover, Efficient Advisors

Todd Colvin, Ambrosino Brothers

Bob Iaccino, Path Trading Partners

Dan Deming, KKM Financial

Bob Iaccino, Path Trading Partners

Larry Shover, Efficient Advisors

Todd Colvin, Ambrosino Brothers

The US Federal Reserve shrugged off concerns about the economic impact of Donald Trump's election victory and moved ahead with a quarter point cut Thursday.

Another year, and the South African love affair with Black Friday continues unabated, albeit not with the same frenzy that greeted it during its peak in 2019, say analysts.

A Jewish family witnesses the power of Jesus when two Christians pray for a sick boy.

Between 17 and 23 December 2010, the team of OM Mexico and a group of volunteers gave away 200 sandals to children in the poor Mexican farming state of Chiapas.

Just under a quarter of the more than 7 000 independent drivers for Shoprite's newly acquired Pingo last-mile delivery platform are South African.

"If we are serious about planting churches among the least reached as how we do mission, we must always be willing to question, reconsider and reform our paradigms," says Shaun Rossi.

A national union for principals is campaigning to increase its membership, drafting in part off the momentum created by the surge in educator activism over the past two years.

Anti-racist school leadership is about becoming more racially aware and taking action, explains Denisa R. Superville.

Two high school softball coaches are immune from a student's privacy lawsuit because there was no clearly established law barring school officials from discussing a student's private matters with the student's parent.

A three-judge panel from the 9th U.S. Circuit Court of Appeals ruled against a proposed plan that would have paid certain student-athletes as much as $5,000 annually in deferred compensation.

As schools plan for fall instruction, educators must take the opportunity to rework math instruction so it's equitable for all students, two math organizations said in a new paper.