New Brunswickers needn't worry about all the buzz around 'murder hornets' because they wouldn't survive the cold winters, says biologist.

Ontario Provincial Police (OPP) have identified the person struck and killed by a train in Kenora on Wednesday as 18-year-old Tyrease Payash, of Kenora.

In this protocol, DNA fragments are separated according to size by electrophoresis through low-melting-temperature agarose, and then recovered by melting the agarose and extracting with phenol:chloroform. The protocol works best for DNA fragments ranging in size from 0.5 to 5.0 kb. Yields of DNA fragments outside this range are usually lower, but often are sufficient for many purposes.

In two-step bacterial artificial chromosome (BAC) engineering, a single plasmid is introduced into the BAC-carrying cell lines. The shuttle vector pLD53.SCAB (or pLD53.SCAEB) carries the recA gene and the R6K origin, which requires the protein to replicate. PIR2 cells, expressing , are typically used for the amplification of the vector and maintain about 15 copies/cell of the donor vector, which is relatively stable in this host.

Holland America and Princess cruise lines issued notices this week cancelling the remainder of the season in Atlantic Canada, citing global health concerns, and that's taking a huge bite out of the Port of Sydney's budget.

President Trump lashes out at the U.S. Postal Service. His attacks could threaten voting by mail; We look at the impact of the coronavirus on schools, universities, students, parents and teachers, and who is at the table to shape what happens next.

As President Trump starts to reopen the country, Pulitzer Prize-winning science writer Laurie Garrett predicts the pandemic will last at least 36 months; Did a former Green Beret mastermind a failed coup attempt in Venezuela? Two Americans were arrested.

Netizens celebrate as Kamal Haasan crosses 6 million mark on Twitter  DNA India

Pittsburgh Penguins superstar Sidney Crosby is easing the burden for many Pennsylvania families struggling during the COVID-19 virus.

Is the Republican indulgence of the president bottomless?

A couple in Montenegro, working with children in a tough neighbourhood in Bar, desire to find ways to reach them with Jesus’ love.

A team of OM workers and local believers share New Testaments and relief packs of food in a Muslim village in Israel.

A story of one OM worker’s struggle, triumph and hope.

When God directed Ellianna to Ireland instead of Austria, she discovered a new opportunity to meet refugees.

OM Costa Rica team members and volunteers share in the joy of giving more than 1,000 indigenous children a dream Christmas.

Farmfoods, a Scottish family business, has served the nation for over 60 years - and has never faltered in selling the finest farm  produce the country has to offer.

The European Union (Withdrawal Agreement) Bill got the Royal Assent this week. It was then solemnly signed, sealed and ratified by Ursula von der Leyen, the new President of the European Commission. We’re finally out.

A five-part series on mistakes made by school administrators is wrapped-up today with commentaries from Dr. Lynell Powell, Stuart Ablon, Alisha Pollastri, Diane Mora and many comments from readers

Leung Wai, from Hong Kong, is burned to pray for Japan after dressing as Santa Claus and being warmly greeted by passers-by last December.

Pertussis is a poorly controlled vaccine-preventable disease. Verifying outbreaks is challenging owing to nonspecific clinical presentations and imperfect diagnostic tests. Exclusive reliance on highly sensitive polymerase chain reaction has been associated with pseudo-outbreaks.

Contamination of specimens with vaccine derived Bordetella pertussis DNA from pediatric clinic surfaces likely resulted in misdiagnoses. Standard practices, liquid transport medium, and lack of polymerase chain reaction cutoffs for discerning weakly positive (contaminant) DNA are contributory, but modifiable factors. (Read the full article)

Recent studies have called into question the benefit of perioperative corticosteroids in children undergoing heart surgery, but have been limited by small sample size, the lack of placebo control, and the grouping of various steroid regimens together in analysis.

We evaluated outcomes across methylprednisolone regimens versus no steroids in a large cohort of neonates and found no mortality or length-of-stay benefit associated with any regimen, and a higher risk of infection in certain subgroups. (Read the full article)

Children with a single kidney are often counseled to avoid contact/collision sports based on the concern of injury to the kidney; however, the incidence of kidney injury during sport is not well understood.

Based on this multiyear, prospective injury surveillance system of varsity-level high school athletes, sport-related kidney injury is rare. Reevaluation of American Academy of Pediatrics recommendations regarding sport participation by children with a single kidney is indicated. (Read the full article)

A reduced nephron number is associated with glomerular hyperfiltration, resulting in renal injury such as hypertension, proteinuria, and chronic kidney disease. Patients with a solitary functioning kidney have an increased risk of dialysis in early adulthood.

This study demonstrates that a subset of children with a solitary functioning kidney progress toward renal injury during childhood. Risk factors for renal injury are ipsilateral anomalies of the kidney and urinary tract and small renal length. (Read the full article)

Active smoking and secondhand smoke are associated with chronic kidney disease in adults. No data are available for children.

Secondhand smoke and active smoking were associated with decreased estimated glomerular filtration rate in US adolescents. These findings support that tobacco smoke effects on kidney function begin in childhood. (Read the full article)

End-stage kidney disease (ESKD) causes significant morbidity and mortality after solid organ transplantation. Adults commonly develop advanced kidney disease, particularly after liver and intestinal transplantation. Previous pediatric studies have not compared the relative incidence of ESKD by organ type.

This national cohort study shows the highest risk of ESKD among pediatric lung and intestinal transplant recipients, reflecting unique organ-specific causes of kidney injury. Our findings have implications for screening for and treating early kidney disease in transplant recipients. (Read the full article)

The demand for donor kidneys for transplantation exceeds supply. En bloc kidney transplantation and donation after determination of circulatory death from pediatric donors increases the potential donor pool.

Newborn infants undergoing elective withdrawal of life support in the NICU are a previously unrecognized source of potential kidney donors. (Read the full article)

Kidney transplantation is the optimal treatment of children with end-stage renal disease. The field of pediatric kidney transplantation has changed over time with regard to immunosuppression, surgical technique, organ allocation policy, and rates of living donor transplantation.

Outcomes after pediatric kidney transplantation in the United States have improved over time, independent of changes in recipient, donor, and transplant characteristics. These improvements were most dramatic within the first posttransplant year and among the most highly sensitized patients. (Read the full article)

The period of transition from childhood to adulthood and the period immediately after transfer of care is a challenging time for young people with kidney failure.

Young patients with kidney failure cared for exclusively in adult-oriented facilities experience increased rates of avoidable hospitalizations during late adolescence and young adulthood. Avoidable hospitalizations increased among pediatric kidney failure patients during the years immediately after transfer to adult care. (Read the full article)

Corrosive substance ingestion is a public health issue in developing countries. Stricture formation is a late complication of corrosive substance ingestion. The role of corticosteroids in preventing corrosive-induced strictures is controversial.

High doses of methylprednisolone therapy lead to less frequent stricture formation in grade IIb esophageal burns in children who ingested caustic substances and may improve prognosis. (Read the full article)

Professional organizations recommend ultrasound as the initial diagnostic imaging modality for children with suspected nephrolithiasis. Computed tomography utilization for children with nephrolithiasis treated at freestanding children’s hospitals is common and varies substantially by hospital.

The high prevalence and regional variability of CT as the first imaging study for children with nephrolithiasis who presented to emergency departments, outpatient clinics, and hospitals throughout the United States indicate that current imaging practices deviate substantially from guidelines. (Read the full article)

Acute kidney injury (AKI) occurs in up to 50% of children after cardiopulmonary bypass and is associated with adverse outcomes. Renal biomarkers have been shown to predict postoperative AKI, but few studies have examined cardiac biomarkers for risk classification.

Preoperative levels of creatine kinase-MB and heart-type fatty acid binding protein are strongly associated with the development of postoperative AKI after pediatric cardiac surgery and can be used to improve preoperative clinical risk prediction. (Read the full article)

Schools should take the lead in removing the stigma around menstruation, writes 16-year-old Maggie Di Sanza, founder of Bleed Shamelessly.

The DnaBand sits on your wrist, scans the barcodes on food, compares it to your DNA, and flashes red if the food is a bad choice and green if it's a healthier option. It's only available in the UK right now, but co-founder Chris Toumazou has his sights set on LA.

CNA Staff, Apr 2, 2020 / 02:50 pm (CNA).- The statue of the Nazarene of Saint Paul will be processed April 8 through the streets of Caracas to help the faithful observe Holy Week.

It will be atop a popemobile used by St. John Paul II when he visited the country in 1985.

According to local tradition, the striking image was brought to Caracas from Seville in 1674. The wooden sculpture depicts Christ dressed in an ornately embroidered purple robe carrying his cross.

According to accounts, the image was processed in the city with prayers during a plague that broke out in Caracas in 1696, and the devotional act was credited with ending the pestilence.

The image was originally kept in a church dedicated to Saint Paul the Hermit, whose intercession was attributed to ending a plague in 1579. The wooden sculpture is now reserved in Saint Teresa Basilica, as Saint Paul’s church was demolished and replaced with a municipal theater by an anticlerical president in 1881.

The procession is held annually on Holy Wednesday.

Cardinal Baltazar Enrique Porras Cardozo, Archbishop of Merida and apostolic administrator of Caracas, said the “route will cover a great part of the city for veneration by its devotees,” and asked for understanding as the route itself has not yet been finalized and will be announced later.

According to local media, the prelate said in a letter that the image should be transported in accordance with safety and hygiene regulations to avoid spreading the coronavirus.

Porras said that the image should not be carried by people but transported by vehicle only and there should be another vehicle for a priest and assistant along with sound equipment for the prayers.

The archdiocese said that parishes can join the initiative and organize such a procession in their own areas as long as they observe the proper health precautions.

Finally, the archdiocese asked the faithful devotees of the Nazarene of Saint Paul to offer their prayers from their homes and to wait for the end of the coronavirus lockdown to visit the image in Saint Teresa Basilica.

Bogotá, Colombia, Apr 3, 2020 / 04:04 am (CNA).- Diana María Toro Vélez was kidnapped on a September day, as she drove home from Mass in the Colombian city where she lived. She spent 453 days in captivity. And she says that God’s grace kept her hope alive during the ordeal.

“I left Mass one Thursday and was driving home in my car and when I got home they assaulted me. They asked me a few questions and took me away. They sold me to the guerrillas,” Toro told ACI Prensa, CNA’s Spanish language news partner.

Toro, the mother of three, told ACI Prensa that during her captivity, she was sometimes forced to march a lot, one time for up to 15 days in a row.

“I clung to God. I cried and prayed a lot. I really held fast to God. I taught the kidnappers how to read and write. We prayed. There was a certain coexistence, and good things came out of the bad,” she said.

Toro, 41, was kidnapped Sept. 27, 2018, in Amagá, about an hour’s drive from Medellin. Her captors were criminals, members of a gang called “The Sorcerers,” who handed her over to Marxist guerrillas from the the National Liberation Army (ELN) for 48M pesos, about $12,000. The ELN reportedly asked 3 billion pesos, about $745,000 for her return.

Toro was released from captivity on Christmas Eve, 2019.

The Catholic Church in Colombia had appealed for her release, and the release appeared to be a goodwill gesture toward achieving some kind of peace agreement between the ELN and the country’s government. Public officials credited the Church with arranging the release.

Speaking to ACI Prensa, the young mom said that she was “very devoted to Our Lady of Guadalupe. When I was kidnapped I made a rosary and prayed it every day, praying a lot to the Virgin that she would allow me to be returned to my children. And God heard me, because I was released on Dec. 24, 2019.”

It was difficult, Toro said, to be “separated from my three children, one of them 3, another 4 and one 14 years old. Separated from my husband, my parents, my siblings, my family members, relatives and friends.”

“These were 453 days of anxiety, grief, sadness and despair. 453 days of living in the middle of the jungle, sleeping under a canopy, on branches, with snakes, scorpions, mosquitos and many other animals around,” she said.

Toro told ACI Prensa she subsisted on parrot, pasta, cooked banana, and rice. She had only two sets of clothes and infrequently bathed. 

“These were really hard days without knowing anything about my family, just with the certainty that God was with me, filling me daily with his strength and fortitude and firmly believing that if I woke up okay, my family was okay too,” she said.

“And God brought me out of that really hard situation. I saw that his glory and his mercy are immense.”

Toro said the ordeal has filled her with gratitude.

“I want to tell those people who in these times feel alone or in despair because of the situation we’re going through in Colombia and the entire world, that God is with us, he never has left us alone, especially now.”

“Let’s pray the rosary, let’s pray as a family. The power of prayer is immense,” she added.

 

This story was first published by ACI Prensa, CNA's Spanish-language news partner. It has been translated and adapted by CNA.

 

CNA Staff, Mar 27, 2020 / 11:19 am (CNA).- Four men working in Iraq for the French humanitarian organization SOS Chrétiens d'Orient who went missing in Baghdad in January have been released by their kidnappers, the French president announced Thursday.

Emmanuel Macron announced March 26 that he “welcomes the release of our three nationals Antoine Brochon, Julien Dittmar, Alexandre Goodarzy and Iraqi Tariq Mattoka.”

The men disappeared Jan. 20 after they made a trip to an appointment by car. SOS Chrétiens d'Orient tried to contact them the following day, unsuccessfully.

The missing employees had gone to Baghdad “to renew their visas and the registration of association with the Iraqi authorities and to monitor the association's operations” in the country.

Macron's office said it had made “every effort” to secure their release, and he expressed “gratitude to the Iraqi authorities for their co-operation.”

SOS Chrétiens d'Orient said last week that they had received no ransom demand, and no group had claimed responsibility for the abduction.

The organization works to support Eastern Christians with humanitarian material aid; it has permanent missions in Syria, Iraq, Lebanon, and Egypt.

Christians in Iraq have suffered persecution in recent years, especially during the invasion of the Islamic State.

Prior to the United States invasion of Iraq in 2003, there were about 1.5 million Iraqi Christians. Today, that number is believed to be fewer than 500,000.

Antofagasta, Chile :: A team of Logos Hope crewmembers pray for patients and their relatives at a hospital.

Others face prison on assault and resisting arrest charges Steven Snell, the Millsboro man who kidnapped and robbed an elderly Rehoboth Beach couple has been sentenced to prison. In July 2018, Snell, 30, went to a home on Cornwall Road asking to use the phone. Once inside, Snell threatened the couple with a box cutter, […]

WILMINGTON, Del. – On Wednesday, November 1, Governor John Carney will host roundtable discussions with leaders in Delaware’s arts & culture, and historic preservation communities. The discussions are part of Governor Carney’s efforts to showcase Delaware as an attractive home for businesses and their employees. Follow Governor Carney’s discussions using the hashtag #OptionsInDE, and learn […]

Citizen coders and civic technologists from across Delaware are set to take on the state’s second annual Open Data Challenge, harnessing the power of public datasets to come up with new solutions to big statewide problems.

Program cancelled due to Hurricane-Florence-related cancellations by panelists.

Historical play about the Delaware patriot by museum historic-site interpreter David Price.

Sharad Malhotra is an Indian film and television actor. He is best known for portraying Rishi Singh Bedi and Sagar in Colors TV's Kasam Tere Pyaar Ki and Zee TV's Banoo Main Teri Dulhann. Currently, he seen in Star Bharat serial Muskaan as Raunak Singh.  <p>Muskaan is an Indian Hindi

To honor the life and legacy of President Abraham Lincoln and in concurrence with an order issued by President Obama, Governor Markell has ordered both the U.S. and Delaware State flags be lowered to half-staff on Wednesday, April 15, 2015.​

Governor Markell has ordered the American and Delaware flags lowered to half-staff on Wednesday, September 2, for the passing of former State Representative Joseph R. Petrilli, Sr.

A wildfire crew led by the Delaware Forest Service is near completion of a two-week assignment on the Fork Complex Fire, a 28,736-acre blaze near Hayfork, California in the Shasta-Trinity National Forest. Almost 2,400 personnel are battling the fire that is currently 26 percent contained. The Fork Complex is one of several large wildfires in Northern California that together cover more than 223,000 acres, one of the major factors that prompted the National Interagency Fire Center (NIFC) to increase its National Preparedness Level today to the maximum of 5 on a 5-point scale.

DOVER – The Delaware Supreme Court, by decision dated Dec. 7, 2017 in the matter of Bridgeville Rifle & Pistol Club, LTD, overturned a Superior Court ruling and ended a ban on visitors’ possessing firearms in Delaware’s state parks and state forests. Pursuant to the ruling, visitors may now possess firearms unless they are prohibited by law due to a past conviction for a violent crime, active Protection from Abuse Order, or mental health commitment. Following the Court’s decision to invalidate the existing regulations limiting firearms in state parks and forests, and some Division of Fish & Wildlife properties, the Delaware Department of Natural Resources and Environmental Control and the Delaware Department of Agriculture (DDA) anticipate issuing interim firearm regulations. The interim regulations would respect the State Constitutional provisions on firearms cited by the Court, by implementing the State’s interest in ensuring the safety of the public in a less restrictive manner.