Suspect Donte West, 28, broke into a side door of the shoe store on Pitkin Ave. near Bristol St. in Brownsville about 8:45 a.m. Saturday and loaded up a Chevy Trailblazer with more than three dozen pairs of sneakers and nearly 40 baseball caps as cops arrived, police said.

The funeral was held for Rav Yosef Kalish, 63, an Amshinover rebbe who was hospitalized with COVID-19 last week and died Sunday morning, The Associated Press reported.

The heartless bandits with hidden faces are wanted for a violent robbery spree across Brooklyn and the Bronx over the past five weeks that includes beating an 83-year-old man, pistol-whipping a woman and shooting a bread deliveryman who survived a bullet to the pancreas.

The scene on 43rd St. between 13th Ave. and 14th Ave. unfolded at about 4 p.m. Thursday as mourners flouted social distancing norms to attend what was supposed to be a private funeral at the home of Rabbi Cheskel Wagshel, 95, said a family friend.

Shivering can activate a series of “heating stations” for your blood vessels—but they take a little while to get up and running.

A touching video of Jane Goodall releasing a chimp into the wild makes my son sob. His tears may reveal frustration over our coronavirus-altered lives.

Danielle Conti, 43, used the pandemic as her personal piggy bank after allegedly stealing the charge card from 70-year-old widower Anthony Catapano while making her daily rounds at hard-hit Staten Island University Hospital North sometime in early April, authorities charge.

Harry Behlin, 46, was arguing Aug. 11 with the two men, Kevin Dillard and Arnelle Branch, when he whipped out a gun and shot them both at the Parkside Houses on White Plains Road near Adee Ave. in Allerton, cops said.

Anaheim Ducks goalie John Gibson pays tribute to Kobe Bryant and the others lost in Calabasas helicopter crash with a specially designed mask.

The frequency of fatal helicopter accidents has slipped in recent decades.

Things were not OK.

He’ll need a better defense than the one his favorite team managed against LSU last year.

Former Xerox researcher Larry Tesler made computers more user-friendly for generations of people with editing commands also including 'find and replace.'

Doesn't really pay for me to be appealing to my captors. They are snarky, and increasingly restless. They pass the long evenings mixing up different flavors of White Claw just for kicks, the way Millennials will.

TV executive Erin Zelle shares what she has learned about herself during the COVID-19 era.

As shelter-in-place orders and long-term quarantine measures reach California, experts tell us what the impact will be on kids, and how to help them.

These are unprecedented times for farmers, accustomed to selling produce to restaurants. Many have shifted to CSA boxes and other methods to cope

Anna Solomon, whose novel "The Book of V." comes out next week, juggles writing, building rafts and book promotion in a void in our latest diary

With concerts cancelled and album releases amiss, musicians with mental health issues are struggling with the stress and uncertainty from the COVID-19 pandemic.

RANGERS new boy Jordan Jones is keen to emulate Cristiano Ronaldo at Rangers - although with the excitement and trickery the Portuguese superstar showed at Manchester United rather than the goalscoring prowess he has produced with Real Madrid and Juventus.

An overview of the Climate Change Conference (also known as COP 17), held in Durban, South Africa in December 2011.

Predictably and sadly, the same issues have resurfaced: lack of media coverage, West stalling on doing anything trying to blame India and China instead, lack of funding, disagreement on how to address it, etc.

Geopolitical threats (real and imaginary) quickly focus a lot of political will and money is easily found to mobilize military forces when needed.

The economy also takes center stage as the current pressing issue, while climate change is easily deferred, in the hopes that the West can let China and India pick up the burden of addressing emissions even though they have not contributed to the historical build up of emissions that have started the recent changes in the climate.

This page is an overview of the Durban conference.

Read full article: COP17 - Durban Climate Conference

An overview of the Climate Change Conference (also known as COP 18), held in Doha, Qatar in December 2012.

Predictably and sadly, the same issues have resurfaced: lack of media coverage, West stalling on doing anything, lack of funding, disagreement on how to address it, etc.

This page is an overview of the Doha Climate conference. It also includes a feed of latest news stories from Inter Press Service’s coverage of the conference.

Read full article: COP18 - Doha Climate Conference

An overview of the Climate Change Conference (also known as COP 19), held in Warsaw, Poland in November 2013.

Predictably and sadly, the same issues have resurfaced: West stalling on doing anything, lack of funding, disagreement on priorities, etc.

This page is an overview of the Warsaw Climate conference and also includes a feed of latest news stories from Inter Press Service’s coverage of the conference.

Read full article: COP19 - Warsaw Climate Conference

An overview of the Climate Change Conference (also known as COP 20), held in Lima, Peru in December 2014.

While it seemed like it was a successful meeting, because developing nations were committed to drawing up their own plans for emissions reductions for the first time, a number of important issues were left undecided such as how financing would work.

This page is an overview of the Lima Climate conference.

Read full article: COP20—Lima Climate Conference

LINE OF DUTY has been keeping BBC viewers hooked for nearly a decade and with the show set to return for its sixth season in the coming months, could one of its key cast members be revealed as a corrupt officer in a huge twist?

LINE OF DUTY has been keeping BBC viewers hooked for nearly a decade and with the show set to return for its sixth season in the coming months, could one of its key cast members be revealed as a corrupt officer in a huge twist?

HOROSCOPE: Horoscopes for this week for all 12 star signs have been shared by Russell Grant. What does the astrology forecast for your sign predict? Check the reading here.

YOU might say the odds have always been stacked against age-gap relationships. The tut-tutters predict doom the moment they so much as sniff a union between May and December. "What does that old fool possibly think that beautiful damsel sees in him?" they ask. "How could that ancient crone believe that hunky young buck finds her attractive?"

The EFL and Premier League could both return in early June after what would, by then, have been a three-month enforced break due to the coronavirus pandemic.

YOU might say the odds have always been stacked against age-gap relationships. The tut-tutters predict doom the moment they so much as sniff a union between May and December. "What does that old fool possibly think that beautiful damsel sees in him?" they ask. "How could that ancient crone believe that hunky young buck finds her attractive?"

How to prevent depression, reduce stress, and increase energy levels while working from home during the coronavirus pandemic.

Future authorizations must be limited in scope and duration so that wars can no longer be waged endlessly without public deliberation, a letter says.

Europeans face a blizzard of restrictions on their freedoms as authorities try to stop the spread.

New techniques are being used to transform images from Hubble into spectacular 3D visualisations.

Why Copper May Become Your Favorite Metal

Even two filmmaking legends can cast the wrong villains amid massive industry change.

Police have launched a manhunt for the perpetrators of the murder of an 89-year-old woman in an old age home in Queenstown in the Eastern Cape.

An alleged robber, who was wounded while his two suspected accomplices were killed in a shootout with police, has been arrested.

A stray cat from Las Vegas has lived to see another day thanks to kindhearted police officers who paused to remove a soup can wedged firmly onto the cat’s head. The lure of a salty, potent-smelling soup can was likely too tempting to resist for the cat who was hooked at the first taste. As…

The post Watch: Cops Save Helpless Stray Cat Found with Head Stuck in Soup Can appeared first on The Western Journal.

A 5-year-old Utah boy shocked family and law enforcement when he grabbed the keys to his parents’ car and drove himself through the neighborhood and onto a freeway. Utah Highway Patrol Trooper Rick Morgan spotted the vehicle swerving dangerously on the freeway and initiated a stop, fully expecting to find a driver under the influence…

The post Cop Gets Shocking Surprise When He Finds Age 5 Boy Behind Wheel of Car Who Had Plans To Go Buy Lamborghini appeared first on The Western Journal.

[jump to video]

Long story (not very) short? One night in 2003--after killing it in front of audience of about 30 lucky people in Oakland--The Long Winters needed a place to crash, and my wife and I were happy to oblige. 

So, they drove their Big Stinky Blue Van over the bridge, slept on our floor, and by breakfast the next morning, it'd become clear to me that I'd provided lodging to a man who was not only very likely a member of my karass--he was also one of the smartest bullshit artists I'd ever met. 

Almost eight years later, although I don't see him nearly as much as I'd like, I still count the guy as one of my best pals ever.

That's John Roderick. And, I think you need to know about him.

John doesn't read this site--he's more of a Twitter person--so I don't risk feeding his astounding excess of dignity by saying he's one of the most gifted writers and bon vivants of our generation. He's just the best. In large part because he's congenitally incapable of suffering bullshit.

This was never more apparent than the Saturday morning in 2007 when we sat in my back yard and talked about a lot of stuff. Playing guitar, advertising on the web, the evil work of promoters, and why everyone is always trying to shortchange everyone on copper pipe. 

That talking became a four-part interview I ran on the late and occasionally lamented The Merlin Show, and, to this day, it's one of my favorite things I've been lucky enough to post to the web.

So, y'know how I'm definitely "not for everyone?" Well, John is really "not for everyone."

He's opinionated and arrogant and undiplomatic and unironically loves Judas Priest--meaning everyone will find at least one thing not to like about him. Despite being hairy and enjoying laying on your bed, John is not exactly a teddy bear. 

But, John's also right a lot. And, he never sands off the edges of his personality or opinions to make you theoretically "like" him. Which, it will come as no surprise to you, is a big reason I love the guy more than a free prime rib dinner. 

So, why the jizzfest about that awful jerk, John Roderick?

Because, as I noted the other day on the Twitter, in our first episode of Back to Work I misattributed a line that should have been credited to John. Which in itself is unimportant, except inasmuch as finding that link to correct the error got me watching our 50-some minutes of chatting again. I also received some at-responses and emails that reminded me how much people enjoyed our chat. 

But, really it made me realize how much that rambling morning in my back yard still resonates so much with stuff I care a lot about. Independence. Agency. Directness. And, never apologizing for wanting to get paid. Also, guitars and talkative hippies.

So, anyway. John. 

I edited all four parts of the video into one big (streamable/downloadable) movie that should make it way easier to watch at a sitting. Should that interest you. Which it may not. Which, as ever, is totally fine, and kind of the point.

But. If you like Dan and my new show (and, seriously—God bless you magnificent bastards who helped briefly make B2W the most popular podcast in the world [gulp]), I think you'll really like this interview a lot too. I hope so, anyway.

Thus, submitted for your disapproval, permit me to present my four-year-old visit with the acerbic, opinionated, and reportedly unlikeable bullshit artist whom I respect and adore more than just about anybody. 

Meet Hotrod.

• Vimeo Page
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The following article, BREAKING: Ex-Cop Father and Son Arrested and Charged With Murder of Black Man Jogging In Neighborhood…President Trump Responds [VIDEO], was first published on 100PercentFedUp.com.

The Georgia Bureau of Investigations has arrested a father and son duo, 64-year-old ex-cop, Gregory McMichael, and his son, 34-year-old Travis McMichael for the February murder of  24-year-old Ahmaud Arbery, a black man who was jogging through their neighborhood when they jumped in their truck and pursued him. Yashar Ali shared the news of the […]

Continue reading: BREAKING: Ex-Cop Father and Son Arrested and Charged With Murder of Black Man Jogging In Neighborhood…President Trump Responds [VIDEO] ...

The following article, BREAKING: President Trump’s Fiery Interview On Fox & Friends…”These are dirty politicians and dirty cops…They put our nation in danger with other nations, including Russia” [VIDEO], was first published on 100PercentFedUp.com.

This morning during a nearly one hour interview with Fox & Friends, President Trump addressed the decision by the DOJ to drop the case against the innocent General Michael Flynn. Trump ripped into the “dirty politicians and dirty cops” who went after General Michael Flynn. President Trump called the players involved in the horrible plot […]

Continue reading: BREAKING: President Trump’s Fiery Interview On Fox & Friends…”These are dirty politicians and dirty cops…They put our nation in danger with other nations, including Russia” [VIDEO] ...

Extracytoplasmic sugar decoration of glycopolymer components of the bacterial cell wall contributes to their structural diversity. Typically, the molecular mechanism that underpins such a decoration process involves a three-component glycosylation system (TGS) represented by an undecaprenyl-phosphate (Und-P) sugar-activating glycosyltransferase (Und-P GT), a flippase, and a polytopic glycosyltransferase (PolM GT) dedicated to attaching sugar residues to a specific glycopolymer. Here, using bioinformatic analyses, CRISPR-assisted recombineering, structural analysis of cell wall–associated polysaccharides (CWPS) through MALDI-TOF MS and methylation analysis, we report on three such systems in the bacterium Lactococcus lactis. On the basis of sequence similarities, we first identified three gene pairs, csdAB, csdCD, and csdEF, each encoding an Und-P GT and a PolM GT, as potential TGS component candidates. Our experimental results show that csdAB and csdCD are involved in Glc side-chain addition on the CWPS components rhamnan and polysaccharide pellicle (PSP), respectively, whereas csdEF plays a role in galactosylation of lipoteichoic acid (LTA). We also identified a potential flippase encoded in the L. lactis genome (llnz_02975, cflA) and confirmed that it participates in the glycosylation of the three cell wall glycopolymers rhamnan, PSP, and LTA, thus indicating that its function is shared by the three TGSs. Finally, we observed that glucosylation of both rhamnan and PSP can increase resistance to bacteriophage predation and that LTA galactosylation alters L. lactis resistance to bacteriocin.

Research Event

Event participants

Research Event

Event participants

In bacteria, the restart of stalled DNA replication forks requires the DNA helicase PriA. PriA can recognize and remodel abandoned DNA replication forks, unwind DNA in the 3'-to-5' direction, and facilitate the loading of the helicase DnaB onto the DNA to restart replication. Single-stranded DNA–binding protein (SSB) is typically present at the abandoned forks, but it is unclear how SSB and PriA interact, although it has been shown that the two proteins interact both physically and functionally. Here, we used atomic force microscopy to visualize the interaction of PriA with DNA substrates with or without SSB. These experiments were done in the absence of ATP to delineate the substrate recognition pattern of PriA before its ATP-catalyzed DNA-unwinding reaction. These analyses revealed that in the absence of SSB, PriA binds preferentially to a fork substrate with a gap in the leading strand. Such a preference has not been observed for 5'- and 3'-tailed duplexes, suggesting that it is the fork structure that plays an essential role in PriA's selection of DNA substrates. Furthermore, we found that in the absence of SSB, PriA binds exclusively to the fork regions of the DNA substrates. In contrast, fork-bound SSB loads PriA onto the duplex DNA arms of forks, suggesting a remodeling of PriA by SSB. We also demonstrate that the remodeling of PriA requires a functional C-terminal domain of SSB. In summary, our atomic force microscopy analyses reveal key details in the interactions between PriA and stalled DNA replication forks with or without SSB.