An automated method for detecting the presence of a nucleic acid in a sample, where the method is performed within a housing of a self-contained, stand-alone analyzer. The method includes purifying the nucleic acid after it has been immobilized on a magnetically-responsive solid support. A pipette of the analyzer is used to form a reaction mixture comprising the purified nucleic acid and all reagents required to perform a nucleic acid amplification. Amplification products are synthesized that include a nucleotide sequence contained in the nucleic acid or the complement of the nucleic acid. The amplification products are exposed to a probe in a mixture, where the probe forms a hybrid with one of the amplification products. The formation of the hybrid in the mixture provides an indication of the presence of the nucleic acid in the sample.

[Problem to be Solved] The importance of sugar chains having α2,3- or α2,6-linked sialic acid at their non-reducing ends is known. Industrial production has been demanded for these sugar chain compounds. Particularly, the production of glycoprotein drugs or the like inevitably requires producing in quantity sugar chains having homogeneous structures by controlling the linking pattern (α2,6-linkage or α2,3-linkage) of sialic acid. Particularly, a triantennary or tetraantennary N-type complex sugar chain having sialic acid at each of all non-reducing ends is generally considered difficult to chemically synthesize. There has been no report disclosing that such a sugar chain was chemically synthesized. Furthermore, these sugar chains are also difficult to efficiently prepare enzymatically.[Solution]The present inventors have newly found the activity of sialyltransferase of degrading sialic acid on a reaction product in the presence of CMP and also found that formed CMP can be degraded enzymatically to thereby efficiently produce a sialic acid-containing sugar chain. The present inventors have further found that even a tetraantennary N-type sugar chain having four α2,6-linked sialic acid molecules, which has previously been difficult to synthesize, can be prepared at high yields by one-pot synthesis comprising the elongation reaction of a biantennary sugar chain used as a starting material without performing purification after each enzymatic reaction.

Process for preparing hydrocyanic acid by catalytic dehydration of gaseous formamide, wherein the dehydration of formamide is coupled with an exothermic reaction by the reactor used in the dehydration comprising two separate fluid paths which are separated by a common reactor wall, with one fluid path being provided for the dehydration of formamide and the second fluid path being provided for the exothermic reaction.

A no-till apparatus for application of herbicide and fertilizer, soil preparation, and seeding of a cover crop in a standing crop has inter-row assemblies configured to pass along inter-row areas between adjacent row lines of standing crop plants. Each assembly includes a fertilizer applicator, a no-till soil preparation element, a cover crop seed applicator, and a post-seeding element.

Methods for preventing or reducing the formation of scale in a wet-process phosphoric acid production process by intermixing a water-soluble functional organic reagent with a phosphoric acid at one or more step of the phosphoric acid production process in an amount sufficient to prevent or reduce at least one species of scale are provided.

A system and method for mitigating an aircraft incident is provided. The invention includes an aircraft panic component coupled to a terminal component. The aircraft panic component facilitates identification of a panic situation and communicates information associated with the panic situation to the terminal component. The aircraft panic component is further operative to at least partially disable a navigation system and/or operational system of an aircraft. The aircraft can then be sent to a safe zone, navigated remotely and/or a course of action for the aircraft can be determined. A safe zone component is adapted to facilitate identification of a course of action for the aircraft based at least in part upon aircraft positional information, aircraft condition information and/or aircraft resource(s). Further, aircraft navigational information and/or aircraft operational information can be sent via an aircraft communication component to a remote system having a remote communication component and a remote analyzing component facilitating transfer of information related to navigational and/or operational system(s) of the aircraft.

There are provided a dividing wall distillation column for producing high-purity acrylic acid, and a fractional distillation method using the same. The dividing wall distillation column includes a condenser, a reboiler and a main column having a dividing wall. Here, the main column is divided into a column-top zone, an upper feed zone, an upper outflow zone, a lower feed zone, a lower outflow zone and a column-bottom zone. Accordingly, since one distillation column can be used to realize the same effect as that obtained from the use of two distillation columns, the dividing wall distillation column can have an effect of reducing the costs of equipment to produce high-purity acrylic acid, as well as an energy-reducing effect, compared to a conventional process system.

A rotary spray head assembly includes a motor having an output shaft and a housing through which the output shaft at least partially extends. The housing includes an inlet communicable with a source of fluid. The rotary spray head assembly also includes an annular skirt extending from the housing, a passageway through the skirt having a first end in fluid communication with the inlet and a second end, and a hub coupled for co-rotation with the output shaft. The hub includes an annular groove into which the annular skirt at least partially extends, a circumferential interior surface at least partially defining the groove, and an aperture through the circumferential interior surface. Fluid introduced into the annular groove through the second end of the passageway is discharged outwardly through the aperture.

The invention provides methods and compositions for increasing the strengthening effect of a starch coating on paper. The method involves contacting the starch with a synthetic polymer before the starch is cooked. This changes how the starch gelatinizes and how the polymer gets distributed on the paper resulting in greater paper surface strength.

A dishwashing detergent composition is provided for consumer use for in cleaning of alkaline sensitive metals such as aluminum or aluminum containing alloys. The compositions include alternatives to sodium tripolyphosphate and/or other phosphorus containing raw materials, while retaining cleaning performance and corrosion prevention. According to the invention, a phosphinosuccinic acid oligomer or mixture thereof is used as a corrosion inhibitor and can be included for aluminum protection in a number of different detergent compositions.

The present invention provides new systems and strategies for the regulation of iron metabolism in mammals. In particular, methods of using agonists and antagonists of TGF-β superfamily members to modulate the expression or activity of hepcidin, a key regulator of iron metabolism, are described. The inventive methods find applications in the treatment of diseases associated with iron overload, such as juvenile hemochromatosis and adult hemochromatosis, and in the treatment of diseases associated with iron deficiency, such as anemia of chronic disease and EPO resistant anemia in end-stage of renal disease. The present invention also relates to screening tools and methods for the development of novel drugs and therapies for treating iron metabolism disorders.

Provided herein are compositions and methods for modifying a predetermined nucleic acid sequence. A programmable nucleoprotein molecular complex containing a polypeptide moiety and a specificity conferring nucleic acid (SCNA) which assembles in-vivo, in a target cell, and is capable of interacting with the predetermined target nucleic acid sequence is provided. The programmable nucleoprotein molecular complex is capable of specifically modifying and/or editing a target site within the target nucleic acid sequence and/or modifying the function of the target nucleic acid sequence.

The present disclosure relates to compositions comprising at least one fluoroolefin and an effective amount of a stabilizer comprising at least one ascorbic acid, terephthalate, or nitromethane, or mixtures thereof. The stabilized compositions may be useful in cooling apparatus, such as refrigeration, air-conditioning, chillers and heat pumps, as well as in applications as foam blowing agents, solvents, aerosol propellants, fire extinguishants, and sterilants.

The present invention relates to dyes of the formula (I) in which R1 to R10, D1 and D2 are defined as given in claim 1, a process for preparing them, and their use for dyeing and printing hydroxyl- and/or carboxamido-containing materials.

As associative thickener obtainable by free radical polymerization, the preparation thereof and the use thereof in paper coating slips are described. The associative thickener is formed from (a) acid monomers selected from ethylenically unsaturated C3- to C8-carboxylic acids, (b) associative monomers of the general formula H2C═CR1—COO-(EO)n—(PO)m—R2, in which R1 is hydrogen or methyl, n is a number of at least two, m is a number from zero to 50, EO is an ethylene oxide group, PO is a propylene oxide group and R2 is a C8-C30-alkyl group or a C8-C30-alkaryl group, and (c) nonionic, copolymerizable monomers differing from a) and b), the reaction product having been reacted, after the polymerization, with initiators forming nonionic radicals.

A method for making a composite polyamide membrane comprising a porous support and a polyamide layer, including the steps of: i) applying a polar solution comprising of polyiunctional amine monomer and a non-polar solution comprising a polyiunctional acyl halide monomer to a surface of a porous support and interfacially polymerizing the monomers to form a polyamide layer; and ii) exposing the thin film polyamide layer to nitrous acid; wherein the method is characterized by at least one of: conducting the interfacial polymerization of step i) in the presence of a subject amine-reactive compound, or applying a subject amine-reactive compound to the interfacially polymerized polyamide layer prior to step ii), wherein the subject amine-reactive compound is different from the polyiunctional acyl halide and polyiunctional amine monomers and is represented by the following formula:

In one example embodiment, a system for treating a tissue site is disclosed comprising a dressing adapted to contact the tissue site and provide a fluid seal between a therapeutic environment and a local external environment, and a solution source fluidly coupled to the dressing and adapted to deliver an antimicrobial solution comprising a peroxy α-keto carboxylic acid, such as peroxy pyruvic acid, to the tissue interface. The system may further comprise a negative-pressure source fluidly coupled to the dressing and adapted to provide negative pressure to the therapeutic environment after delivery of the antimicrobial fluid to the therapeutic environment. In another example embodiment, a method for treating a tissue site is disclosed comprising positioning a tissue interface to contact the tissue site, covering the tissue interface and the tissue site with a drape to provide a fluid seal between the therapeutic environment and the local external environment, and delivering an antimicrobial solution comprising peroxy α-keto carboxylic acid to the therapeutic environment before providing negative pressure to the therapeutic environment.

A method for preparing an object to be tested, having a given relative permittivity, intended to be illuminated by an incident electromagnetic wave. The method includes: providing a part including a cavity for housing the object and at least one extension element made from a material having a relative permittivity that is preferably equal to that of the object, the extension element at least partially delimiting the cavity and extending to either side of the cavity in a passage direction of the cavity, over a length at least equal, on either side of the cavity, to one third of the length of the cavity in the passage direction, and placing the object in the cavity, such that the object is in contact with the extension element in the passage direction.

Disclosed is a piezoelectric material including stereocomplex crystals of poly(D-lactic acid) (PDLA) and poly(L-lactic acid) (PLLA), wherein poly(D-lactic acid) and poly(L-lactic acid) are crystallized into a stereocomplex, thus exhibiting superior heat resistance and piezoelectric properties. This piezoelectric material, which is obtained using poly(lactic acid), can be produced at very low cost compared to when using PVDF, and is configured to include stereocomplex crystals of PDLA and PLLA and can thus manifest high thermal stability and piezoelectric properties, compared to when using conventional PLLA alone. Such a piezoelectric material can be efficiently utilized in a variety of fields in which low production cost, high processing temperature in the manufacturing process, or high-temperature stability of piezoelectric material products is required.

Use of a reaction product of saturated or unsaturated aliphatic mono- or polycarboxylic acids with aliphatic polyamines for improving or boosting the separation of water from fuel oils which comprise additives with detergent action. A Fuel additive concentrate comprising the said reaction product, certain additives with detergent action and optionally dehazers, cetane number improvers and solvents or diluents.

The present invention relates to a method, device and computer program for terminating a defrosting cycle within a refrigerated transport container. The container may include: a transport volume, a cooling unit comprising an evaporator arranged in a cooling space, a return air grid arranged to separate said cooling space from said transport volume, means for sensing temperature indicative of the return air temperature of air returning to said cooling space from said transport volume or the temperature of the return air grid, means for actively heating said evaporator during defrosting cycles, and a processor configured for controlling the duration of said defrosting cycles. The method includes: establishing an indicator(s) indicative of frost and/or ice build-up on said return air grid, and terminating a defrosting cycle when an indicator(s) of frost and/or ice build-up on said return air grid indicates that said return air grid is free of frost and/or ice.

POLICE are appealing for witnesses following a serious incident.

AN UNDER-FIRE health trust has admitted it missed chances to intervene when a mentally ill Southampton man was in “crisis”.

POLICE are appealing for witnesses following a serious incident.

LEISURE bosses are today bracing themselves to make the next step towards the development of a huge, multi-million-pound, Las Vegas-style casino planned for Southampton.

Dive in with Thomas Domville as he listens to the LIVE actions as they happen with Scanner Radio for iOS. A free app that you don't want to miss out that brings a wide range of scanner feeds to follow and listen such as Police, Fire, EMS, Railways, Aviation, Marine and others that may interest you.

Scanner Radio: Police & Fire on the App Store
https://apps.apple.com/us/app/scanner-radio-deluxe/id498405045

Delaware County Sheriff's deputies say they have solved the 36-year-old murder of a teen.

Ari Shaffir, Comedian, and Host of the, "Skeptic Tank" podcast, Eddie Bravo, 10th Planet Jiu Jitsu,join Joey Diaz and Lee Syatt in studio with a call from Duncan Trussell, 'The Duncan Trussell Family Hour"

A criminal investigation is underway after the death of an inmate at the Arizona State Prison Complex in Yuma on Thursday.

Zurich-based Committee International d'Esthtique et de Cosmtologie (CIDESCO) is considered world standard for Beauty and Spa Therapy.

Andy Murray does not think getting the professional tennis circuit back up and running should be a priority any time soon.

IT’S been six weeks since we went into lockdown and even though we have a long way to go until any normality is to return, it was so good to hear that the Scottish Government has started to consider what the future would look like for us when the time comes to ease restrictions.

In northern Italy, doctors were forced to begin rationing ventilators and other equipment—a nightmare scenario that could become a reality for medical staff in the United States soon; New York has projected ventilator shortages in the thousands per week. David Remnick talks with Philip Rosoff, a professor of Medicine at Duke University and a scholar of bioethics who has studied rationing. Rosoff believes medical institutions must also consider the needs of those who can’t be saved, and suggests that hospitals should stock up on drugs to ease suffering at the end of life. Rosoff notes that the U.S. medical system puts an emphasis on “go for broke” care at all costs, and is poorly prepared for those kinds of decisions, which leave hospital workers with an acute sense of “moral distress.” “If we’re smart, we would have institutional guidelines and plans in place ahead of time,” Rosoff says. “The way not to make [a rationing decision] is to make it arbitrarily, capriciously, unilaterally, and at the bedside in the moment.”

Four and a half years ago, I thought I was a cis man. Then I wrote a book.

Lucid is the tale of Jordan Parker (Parker to her friends), a trans girl who gets super powers and the body she's always wanted. As I wrote the book, I felt ever moment of her euphoria and getting to be herself. I felt her pain when she her true self was denied. Somewhere before I wrote that last line, I realized that I was trans.

I tried for a bit to get the book published, but that didn't go anywhere. A couple weeks ago I didn't want to leave Parker sitting on a shelf any more. She shows me who I am. She deservers better than that. So I started posting her novel online.

I hope some of you enjoy it.

[Link]

***This episode references guns and suicide and may be upsetting for some of our listeners*** Data can be a tricky thing to bring into discussions around guns and suicide. As Dr. Art Markman and Dr. Bob Duke discuss in this episode of Two Guys on Your Head, being more critical about the way information is...

In the 1970s Monsanto unveiled a miracle herbicide–Glyphosate. The pitch: it was as safe as table salt for people, but could flatten even the peskiest weeds. Farmers and homeowners alike have used the product ever since. Now, it shows up in detectable levels in many foods, and almost every American has some in their bodies....

"Estamos muy complacidos porque el diario AS es el líder entre los medios deportivos" Sarah Castro

Washington State football player Bryce Beekman died in his Pullman apartment on March 23 from ‘acute intoxication’ of fentanyl and promethazine, the Whitman County Coroner's Office said Friday.

Many of the country’s struggles before the pandemic — including mass internal migration, unsafe workplaces and industrial disasters — have been amplified by the lockdown and the subsequent move to reopen businesses.

Emerald City Comic Con organizers said Wednesday morning that they will change their policy and allow refunds to fans who choose not to attend this year because of coronavirus concerns.

Washington State football player Bryce Beekman died in his Pullman apartment on March 23 from ‘acute intoxication’ of fentanyl and promethazine, the Whitman County Coroner's Office said Friday.

Earl Thomas was not arrested in the incident, TMZ reported. Nina Thomas, according to the report, was arrested for burglary of a residence with intent to commit aggravated assault. She was released on bond.

The $300,000 effort could take place between now and June depending on weather conditions and the moths' hatching season, officials said.

The longtime boys and girls basketball coach was named the athletic director.

Earl Thomas was not arrested in the incident, TMZ reported. Nina Thomas, according to the report, was arrested for burglary of a residence with intent to commit aggravated assault. She was released on bond.

Available for viewing online. Do we have the right to end our lives if life itself becomes unbearable, or when we enter the late-stages of painful, terminal illness? The questions, debated for centuries, have only grown more pressing in recent years as medical technology has allowed us to live longer lives, and several U.S. states have legalized physician-assisted suicide. With unique access to Dignitas, the Swiss non-profit that has helped over one thousand people die since 1998, Academy award- winning filmmaker John Zaritsky offers a revealing look at a couple facing the most difficult decision of their lives--and lets us see for ourselves as one Chicago native makes the trip to Switzerland for what will become the last day of his life.

Facebook on Wednesday announced the first members of its new Oversight Board, which will make final decisions about what content should be allowed or removed from Facebook and Instagram, even if the company or Chief Executive Mark Zuckerberg disagree. The Oversight Board represents a new model of content moderation for Facebook as well as Instagram.

The mother of Perth girl Denishar Woods, left with severe brain damage after a severe electric shock, has been waiting for a report into the accident, but it fails to establish who is to blame.

Jayden Whyte tried to get help twice on the day he died. Now he is being remembered through a striking grassroots project that could help others before it's too late.