velocityconf: S Bay Area #WomeninTech (+ allies!) pls come to the #VelocityConf women's communities meetup on 6/19 http://t.co/xSY7jcyPXV

While at least 19 000 inmates inside South Africa's prisons will be eligible for special parole to curb the spread of Covid-19, those sentenced for a range of serious crimes will not make the cut.

Arsene Wenger says Liverpool will be regarded as 2019/20 Premier League winners even if the season is abandoned because of the coronavirus.

Netcare St Augustine's and Netcare Kingsway hospitals have been given the green light to reopen.

News24 asked young people to send to us their reasons for leaving and what would have to be different for them to return to South Africa, or not leave at all. The response was overwhelming.

It is at times like these that we as a country rely on the relevant stakeholders to take care of our people and put into action the promises made in the preamble of our Constitution, particularly where it is stated that through our freely elected representatives, the quality of life of all citizens is to be improved.

Are you dreaming of being your own boss someday, or would you like to bring in extra income? Starting an online business is one of the easiest ways to accomplish this. The biggest challenge of starting an online business can be developing your business plan. If you are struggling to come up with that brilliant […]

From smartphone-operated elevators to contactless coffee machines, businesses are trying to eliminate major touchpoints to help deter coronavirus spread in the workplace.

Many homeowners have built up wealth in their homes. Owners’ balance sheets remained strong in the first quarter across the U.S., a new report shows.

After a month-long hiatus, additional instant offer firms are stepping back in to purchase homes, using contact-free processes.

Ashland, Oregon Shakespeare Festival

Rerun - Cruising - Barefoot or Formal

Theme Cruise to Panama (Shakespeare at Sea / MacMania)

The Amateur Traveler compares two very different cruise options, a Royal Caribbean cruise with formal nights and midnight buffets and the Windjammer Barefoot Cruise. Which cruising option is right for you?

Hear about travel to Bucharest as the Amateur Traveler talks to Wandering Earl about visiting and living in that city. Earl calls Bucharest one of the most under-rated cities in all of Europe, certainly eastern Europe.

Hear about travel to Arequipa, Peru as the Amateur Traveler talks to Sam from Indefinite Adventure. Arequipa is Peru’s second largest city after Lima. It is in the south, inland, west of Lake Titicaca and south of Cusco. Sam heard about Arequipa from other travelers and lived and worked there for a month as a temporary local.

Hear about travel to Delaware as the Amateur Traveler talks to Mara Gorman from MotherOfAllTrips.com about traveling to her small but historic adopted home state.

At a cost of $7.5 million a patient, they were 16 very expensive field hospitals. Yet, according to NPR, those hospitals are now “stand[ing] down.” You probably remember them from headlines early in the pandemic: makeshift medical centers being assembled at breakneck speed by companies contracted by the Army Corps of Engineers in anticipation of…

The post After Treating Barely Any Patients for a Massive $7.5 Million Each, 16 Emergency COVID Hospitals Are Standing Down appeared first on The Western Journal.

CTVNews.ca asked Canadians to share how they were using their CERB payments and got a flood of responses. Most said they were covering the basics -- housing, groceries, transportation and medicine.

The Justice Department has intervened in troubled police departments for 20 years. Are reform efforts working?

It appears ISIS-allied fighters are gaining a foothold in Afghanistan, but just how similar are they to the group's branches in Iraq and Syria?

For many low income college students, school closures due to COVID-19 has affected the way they feed themselves. Read more...

'Hollywood' actor Samara Weaving lets us in on all of the movies and television she's been watching during quarantine.   Read more...

Zoom has become a way of life for some people as of late (despite, uh, some safety concerns), especially when it comes to work meetings. Whether it's a company-wide all hands or a team check-in, it's likely that if you're working from home due to the coronavirus pandemic, you've had to see your coworkers' shining faces in a video call. 

Actor, writer, and Groundlings comedian Caitlin Reilly perfectly encapsulated that one co-worker you just can't stand during these video meetings:

It's so relevant it hurts. Who hasn't heard the phrase "to piggyback off of" during one of these calls?

The inspiration behind the 'tok was experiences Reilly had sitting in Zoom meetings on behalf of her boss. "I had actually never been in a zoom meeting before, so it was funny for me to see all the different 'personality' types in a situation like that," Reilly told Mashable. "And so it popped in my head one day and I just made the video." Read more...

It took hundreds of exposures and a complex method of removing occlusions, but this "lucky" shot is the result: a depiction of the hot regions lurking under Jupiter's uppermost clouds.

The picture was captured in infared by the Gemini North Telescope in Hawaii, and is one of the sharpest observations of the planet ever made from the ground. To achieve the resolution, scientists used a technique called "lucky imaging" which scrubs out the blurring effect of looking through Earth's turbulent atmosphere. This method involves acquiring multiple exposures of the target and only keeping those segments of an image where that turbulence is at a minimum.

Who is Patrick Mason? I just ran across a few short horror films he wrote and directed, and they're truly scary. Like edge-of-your-seat gasp-out-loud scary. Not only that, but they're beautifully made with good actors, especially Ayuda (see below). The three videos posted here are the ones I've seen so far, but there are more on his site, which I plan to watch tonight. Can't wait to see where this director takes us next.

Read the rest

If you’re an entrepreneur or marketer who hasn’t embraced webinars yet, you probably need to rethink your strategies. Over 60 percent of marketers say they use webinars as a key part of their content marketing strategy — and over 70 percent say it’s the best way to generate high-quality business leads.

Thankfully, one of the silver linings to our new quarantine, work from home lifestyles lately is the explosion of conferencing software and Americans’ newfound enthusiasm for video group meetups. 

Vidthere is one of the services that has considered the needs of large and small groups trying to maintain connections over distance, offering a suite of web-based communication tools that centralizes everything in one easy-to-use place.

Vidthere is a live video platform for everyone, featuring loads of internal communications features as well as options to sell and market directly to customers.

With webinars emerging as a key means to engage customers and sell products, Vidthere gives you all the tools to do that from any location. Vidthere offers the opportunity to deliver live video webinars that are easy for both presenters and users. 

Vidthere is entirely web-based, so users never need to download any software to join a Vidthere session. As for sessions, they combine no-lag high-quality performance with the ability to scale to the size of an audience with no video loss. Plus, every Vidthere meeting or webinar has a powerful chat feature so users can engage easily.

As for presenters, Vidthere events support up to 30 meeting participants and an unlimited number of webinar viewers, with options to support screen sharing, video in video presentation, and a whiteboard mode, a full basket of tools to help contour any presentation just the way you want it. Read the rest

My pal Jake has been taking regular "cigar walks" (as he calls them) here in our lovely island city of Alameda, California. On those walks, he started spotting some creative flyers...

(I saved the best two for last...)

photos by Jake Schaffer, used with permission

Thanks, Susie! Read the rest

Peter Kay’s Car Share Synopsis Peter Kay’s Car Share is a situation comedy television series produced by BBC One that premiered in April of 2015. Peter Kay’s Car Share follows the story of John (played by Peter Kay), an assistant manager in a supermarket and his co-worker and promotions rep Kayleigh (played by Sian Gibson). […]

Rare coins can add beauty and value to any collection. It is a piece of history that any man or woman hold in their hand. It could belong to an ancient era and certain empires such as the Romanian world or maybe it’s a silver dollar that pioneered in the wild east. Coin collectors are […]

The post Start Your Coin Collection With These Rare Coins appeared first on Dumb Little Man.

Digital marketing is fast-paced with trends and new updates to existing systems continuously popping out. That’s why it is important to stay up-to-date with the latest strategies that can be beneficial in reaching new audiences. Keeping up to date with the marketing trends will also help a business stay ahead of their competition. The best […]

The post Five of the Best B2B Content Marketing Trends That Are Proven Effective To Gain New Clients appeared first on Dumb Little Man.

Dan Greenberg, co-founder and CEO of native advertising solutions provider Sharethrough, discusses how buyers’ increased desire for contextual targeting and native advertising will benefit premium publishers in 2018.

May 6, 2020 (New York, NY) – “Insider Intelligence” is being announced today as the brand name of the newly-formed parent company of eMarketer and Business Insider Intelligence (BII), both […]

For those long, long travel days when you can't be bothered to change your socks ... you need the odorless, antibacterial MP Magic Socks.

The post Are These “Magic” Socks the Best Odorless Socks for Travelers? appeared first on Vagabondish.

We’ve been teaching people how to do remote work well for the better part of two decades. We wrote a whole book about the topic in 2013, called REMOTE: Office Not Required. Basecamp has been a remote company since day one, and our software is sold as an all-in-one toolkit for remote work. Yeah, we’re… keep reading

Merlin Mann - "Scared Shitless: How I (Mostly) Learned to Love Being Afraid of Pretty Much Everything"

Download MP4 Video of "Scared Shitless"

This is the video of a talk I did last month at Webstock in Wellington, New Zealand.

It's pretty different from a lot of stuff I've done. It's about being scared.

As I mentioned on Back to Work, Webstock is—what? Well. Webstock is unique. Truly. If you get the chance, you should go. Really.

I could not and would not have done this talk in this way had I had not been so inspired (and, frankly, so terrified) by the awesomeness of the other speakers, by the quality of their talks, and by the astounding graciousness and empathy of the audience that this particular event attracts.

Tash and Mike and their crackerjack team have made something really special here. I'm honored that they even invited me, and I'm insanely grateful for the care and hospitality that they showed to the speakers and to the attendees at every step of the way.

Seriously. Thank you.

So, yeah. I did something really weird at Webstock. Weird for me and, honestly, just plain weird for "a talk."

I'm not sure if it succeeded. But, I did the best I could to make myself (along with some really heroic friends and fellow speakers) into a legitimate guinea pig for a concept that means the world to me:

You can be scared and still do it anyway. Regardless of whatever it is.

And, you can. No. Really. You. You can do this.

You can run toward the shitstorm, let it cover you with shit, but, still never let it stop you from running.

Because, like Crazy Bob says:

"They can't eat you."

And, they can't. And, they won't. Okay?

Well, okay, then.

Nurses, PSWs, and front-line workers in long-term care and retirement homes have been dealing with the full reality of this pandemic every day.

The following article, Donald Trump Jr. Offers To Walk Bride-to-Be Down The Aisle After Liberal Parents Refuse To Attend Her Wedding Because Fiance Is A Conservative, was first published on 100PercentFedUp.com.

TDS or Trump Derangement Syndrome brings out the worst in a lot of people. One young Texan girl’s story of her intolerant parents who refuse to attend her wedding because she’s marrying a conservative, however, is a new low. Lawyer and conservative civil rights activist, Rogan O’Handley tweeted a Tik Tok video of a Texan […]

Continue reading: Donald Trump Jr. Offers To Walk Bride-to-Be Down The Aisle After Liberal Parents Refuse To Attend Her Wedding Because Fiance Is A Conservative ...

The following article, BREAKING: President Trump’s Fiery Interview On Fox & Friends…”These are dirty politicians and dirty cops…They put our nation in danger with other nations, including Russia” [VIDEO], was first published on 100PercentFedUp.com.

This morning during a nearly one hour interview with Fox & Friends, President Trump addressed the decision by the DOJ to drop the case against the innocent General Michael Flynn. Trump ripped into the “dirty politicians and dirty cops” who went after General Michael Flynn. President Trump called the players involved in the horrible plot […]

Continue reading: BREAKING: President Trump’s Fiery Interview On Fox & Friends…”These are dirty politicians and dirty cops…They put our nation in danger with other nations, including Russia” [VIDEO] ...

The provincial government has announced it will support child care centres that have been closed since March with their fixed operating costs as the fight against COVID-19 continues.

Health officials with the Manitoba government announced on Friday that a COVID-19 outbreak at the Health Sciences Centre has officially been declared over.

Environment Canada has issued a special weather statement for the southwestern area of Manitoba.

Assembled α-synuclein in nerve cells and glial cells is the defining pathological feature of neurodegenerative diseases called synucleinopathies. Seeds of α-synuclein can induce the assembly of monomeric protein. Here, we used sucrose gradient centrifugation and transiently transfected HEK 293T cells to identify the species of α-synuclein from the brains of homozygous, symptomatic mice transgenic for human mutant A53T α-synuclein (line M83) that seed aggregation. The most potent fractions contained Sarkosyl-insoluble assemblies enriched in filaments. We also analyzed six cases of idiopathic Parkinson's disease (PD), one case of familial PD, and six cases of multiple system atrophy (MSA) for their ability to induce α-synuclein aggregation. The MSA samples were more potent than those of idiopathic PD in seeding aggregation. We found that following sucrose gradient centrifugation, the most seed-competent fractions from PD and MSA brains are those that contain Sarkosyl-insoluble α-synuclein. The fractions differed between PD and MSA, consistent with the presence of distinct conformers of assembled α-synuclein in these different samples. We conclude that α-synuclein filaments are the main driving force for amplification and propagation of pathology in synucleinopathies.

Research Event

Event participants

David Roberts, Assistant Professor and School of Security Studies Lead for Regional Security and Development, King's College London
Kristian Coates Ulrichsen, Associate Fellow, Middle East and North Africa Programme, Chatham House
Chair: Sanam Vakil, Deputy Director and Senior Research Fellow, Middle East and North Africa Programme, Chatham House

Extracytoplasmic sugar decoration of glycopolymer components of the bacterial cell wall contributes to their structural diversity. Typically, the molecular mechanism that underpins such a decoration process involves a three-component glycosylation system (TGS) represented by an undecaprenyl-phosphate (Und-P) sugar-activating glycosyltransferase (Und-P GT), a flippase, and a polytopic glycosyltransferase (PolM GT) dedicated to attaching sugar residues to a specific glycopolymer. Here, using bioinformatic analyses, CRISPR-assisted recombineering, structural analysis of cell wall–associated polysaccharides (CWPS) through MALDI-TOF MS and methylation analysis, we report on three such systems in the bacterium Lactococcus lactis. On the basis of sequence similarities, we first identified three gene pairs, csdAB, csdCD, and csdEF, each encoding an Und-P GT and a PolM GT, as potential TGS component candidates. Our experimental results show that csdAB and csdCD are involved in Glc side-chain addition on the CWPS components rhamnan and polysaccharide pellicle (PSP), respectively, whereas csdEF plays a role in galactosylation of lipoteichoic acid (LTA). We also identified a potential flippase encoded in the L. lactis genome (llnz_02975, cflA) and confirmed that it participates in the glycosylation of the three cell wall glycopolymers rhamnan, PSP, and LTA, thus indicating that its function is shared by the three TGSs. Finally, we observed that glucosylation of both rhamnan and PSP can increase resistance to bacteriophage predation and that LTA galactosylation alters L. lactis resistance to bacteriocin.

Nucleoside analogues are a valuable experimental tool. Incorporation of these molecules into newly synthesized DNA (i.e. pulse-labeling) is used to monitor cell proliferation or to isolate nascent DNA. Some of the most common nucleoside analogues used for pulse-labeling of DNA in cells are the deoxypyrimidine analogues 5-ethynyl-2'-deoxyuridine (EdU) and 5-ethynyl-2'-deoxycytidine (EdC). Click chemistry enables conjugation of an azide molecule tagged with a fluorescent dye or biotin to the alkyne of the analog, which can then be used to detect incorporation of EdU and EdC into DNA. The use of EdC is often recommended because of the potential cytotoxicity associated with EdU during longer incubations. Here, by comparing the relative incorporation efficiencies of EdU and EdC during short 30-min pulses, we demonstrate significantly lower incorporation of EdC than of EdU in noninfected human fibroblast cells or in cells infected with either human cytomegalovirus or Kaposi's sarcoma-associated herpesvirus. Interestingly, cells infected with herpes simplex virus type-1 (HSV-1) incorporated EdC and EdU at similar levels during short pulses. Of note, exogenous expression of HSV-1 thymidine kinase increased the incorporation efficiency of EdC. These results highlight the limitations when using substituted pyrimidine analogues in pulse-labeling and suggest that EdU is the preferable nucleoside analogue for short pulse-labeling experiments, resulting in increased recovery and sensitivity for downstream applications. This is an important discovery that may help to better characterize the biochemical properties of different nucleoside analogues with a given kinase, ultimately leading to significant differences in labeling efficiency of nascent DNA.

Dame Margaret Anstee, the first female UN under-secretary general, who died last year after a long and distinguished career as a diplomat, was remembered this week at Chatham House.

12 May 2016