Veterans are being hit hard by unemployment due to the coronavirus pandemic.

Veteran Broadway actor, best known for playing Roy Biggins on "Wings," has died in New York at 73.

Hal Willner, Grammy-winning record producer and longtime "Saturday Night Live" sketch music producer, has died from complications from COVID-19. He was 64.

"right to literacy": children learn to read mostly at home

In Tarzana, Houston Rockets guard Ben McLemore is shooting for $2.799 million for his East Coast-inspired home of two years.

Amber Havard and Rob Morgan co-star in "Bull," a drama about a troubled teen in a poor Houston neighborhood who comes under the tutelage of her neighbor.

LOCKDOWN has turned many people into quiz fans and for those compiling quizzes here are some book-related questions for your home pub quiz.

LOCKDOWN has turned many people into quiz fans and for those compiling quizzes here are some book-related questions for your home pub quiz.

KATE and Prince William took part in a surprise party over video call to celebrate the 75-year anniversary of VE Day.

Police said the pregnant woman was hit on South Harding Street, near the I-465 ramp.

The series includes 26 drivers ages 40 and up who either made their names in racing decades ago or are still in the cockpit.

Honoring those who serve

Steve Brunes spent nearly four decades coaching Indiana high school basketball with stops at Brownsburg, Cowan, Columbus East, Castle and Alexandria.

Mapa interativo mostra medidas que países vizinhos vêm tomando desde primeiros casos registrados de covid-19 para restringir movimento dos cidadãos.

Which Colts incumbents benefited the most from an offseason of change? And who's now in a tougher position than they were at season's end?

Get a sneak peek inside the Festival Country Visitor Center, Johnson County's tourism hub in Franklin. The center opens to the public March 17.

The new Johnson County visitor center in downtown Franklin features interactive exhibits. Each exhibit highlights things to do around the county.

Flt Lt Terry Clark, who was a radar operator during WW2, died on the eve of the VE Day anniversary.

Albert Jarrett was among some 10,000 West Indians who crossed the Atlantic to join the war effort.

The 75th anniversary of VE day gave Canadians a chance to remember and pay their respect to those that served in World War II.

Orange Slovensko the leading telco operator on the market uses the nangu.TV Media Platform for IPTV and OTT delivery. "With the growing demand for interactive television and more viewing possibilities, Orange sought a proven solution to bring to the market," says Product Manager at Orange, Martin Hainzl.

Orange offers their customers TV with value added features, which gives them more options and comfort than classic live TV. The VoD selection stores 50 movie titles, accessible on multiple screens: TV, PC, Smartphone or tablet. The TV Archive allows playback of content up to 7 days in the past. Customers can utilize the time shift feature to browse content already aired and watch it at their leisure with pause, play and star t over commands. They have flexibility in watching television on the go with the newly released applications for both iOS and Android devices. The nangu.TV solution allows operators to stay ahead of competition by giving cutomers the chance to be in control of how they watch TV.

Teaching students to read is the first job of our schools. How can we help all students become strong, confident readers? Literacy for All: Equitable Practices for Reading and Dyslexia was held on February 20, 2020 in Annapolis, Maryland. The event was hosted by National Center on Improving Literacy (NCIL), Decoding Dyslexia Maryland (DD-MD), and community partners. Reading experts from NCIL shared best practices in early screening and interventions supported by decades of reading research.

Hello, Reading World! As with most of you, I’m sheltering in place … biding my time until the Great Pandemic Pandemonium subsides. Although despite being at what is currently an awkward (and apparently dangerous) age, I feel pretty safe locked down here in Chicago. Nevertheless, like all of you, I'm worried about family members who are on the front line in this fight, my students and colleagues, and all the people who are taking care of us. 

Teaching students to read is the first job of our schools. How can we help all students become strong, confident readers? Literacy for All: Equitable Practices for Reading and Dyslexia was held on February 20, 2020 in Annapolis, Maryland. The event was hosted by National Center on Improving Literacy (NCIL), Decoding Dyslexia Maryland (DD-MD), and community partners. Reading experts from NCIL shared best practices in early screening and interventions supported by decades of reading research.

Hello, Reading World! As with most of you, I’m sheltering in place … biding my time until the Great Pandemic Pandemonium subsides. Although despite being at what is currently an awkward (and apparently dangerous) age, I feel pretty safe locked down here in Chicago. Nevertheless, like all of you, I'm worried about family members who are on the front line in this fight, my students and colleagues, and all the people who are taking care of us. 

Teaching students to read is the first job of our schools. How can we help all students become strong, confident readers? Literacy for All: Equitable Practices for Reading and Dyslexia was held on February 20, 2020 in Annapolis, Maryland. The event was hosted by National Center on Improving Literacy (NCIL), Decoding Dyslexia Maryland (DD-MD), and community partners. Reading experts from NCIL shared best practices in early screening and interventions supported by decades of reading research.

After a long battle with cancer, Bollywood actor Rishi Kapoor has died.

Previous studies have shown that sphingosine kinase interacting protein (SKIP) inhibits sphingosine kinase (SK) function in fibroblasts. SK phosphorylates sphingosine producing the potent signaling molecule sphingosine-1-phosphate (S1P). SKIP gene (SPHKAP) expression is silenced by hypermethylation of its promoter in acute myeloid leukemia (AML). However, why SKIP activity is silenced in primary AML cells is unclear. Here, we investigated the consequences of SKIP down-regulation in AML primary cells and the effects of SKIP re-expression in leukemic cell lines. Using targeted ultra-HPLC-tandem MS (UPLC-MS/MS), we measured sphingolipids (including S1P and ceramides) in AML and control cells. Primary AML cells had significantly lower SK activity and intracellular S1P concentrations than control cells, and SKIP-transfected leukemia cell lines exhibited increased SK activity. These findings show that SKIP re-expression enhances SK activity in leukemia cells. Furthermore, other bioactive sphingolipids such as ceramide were also down-regulated in primary AML cells. Of note, SKIP re-expression in leukemia cells increased ceramide levels 2-fold, inactivated the key signaling protein extracellular signal-regulated kinase, and increased apoptosis following serum deprivation or chemotherapy. These results indicate that SKIP down-regulation in AML reduces SK activity and ceramide levels, an effect that ultimately inhibits apoptosis in leukemia cells. The findings of our study contrast with previous results indicating that SKIP inhibits SK function in fibroblasts and therefore challenge the notion that SKIP always inhibits SK activity.

Marilyn Goudreault
Jan 1, 2009; 8:157-171
Research

Sean R. Collins
Mar 1, 2007; 6:439-450
Research

The respiratory epithelium comprises polarized cells at the interface between the environment and airway tissues. Polarized apical and basolateral protein secretions are a feature of airway epithelium homeostasis. Human respiratory syncytial virus (hRSV) is a major human pathogen that primarily targets the respiratory epithelium. However, the consequences of hRSV infection on epithelium secretome polarity and content remain poorly understood. To investigate the hRSV-associated apical and basolateral secretomes, a proteomics approach was combined with an ex vivo pediatric human airway epithelial (HAE) model of hRSV infection (data are available via ProteomeXchange and can be accessed at https://www.ebi.ac.uk/pride/ with identifier PXD013661). Following infection, a skewing of apical/basolateral abundance ratios was identified for several individual proteins. Novel modulators of neutrophil and lymphocyte activation (CXCL6, CSF3, SECTM1 or CXCL16), and antiviral proteins (BST2 or CEACAM1) were detected in infected, but not in uninfected cultures. Importantly, CXCL6, CXCL16, CSF3 were also detected in nasopharyngeal aspirates (NPA) from hRSV-infected infants but not healthy controls. Furthermore, the antiviral activity of CEACAM1 against RSV was confirmed in vitro using BEAS-2B cells. hRSV infection disrupted the polarity of the pediatric respiratory epithelial secretome and was associated with immune modulating proteins (CXCL6, CXCL16, CSF3) never linked with this virus before. In addition, the antiviral activity of CEACAM1 against hRSV had also never been previously characterized. This study, therefore, provides novel insights into RSV pathogenesis and endogenous antiviral responses in pediatric airway epithelium.

In bacteria, the restart of stalled DNA replication forks requires the DNA helicase PriA. PriA can recognize and remodel abandoned DNA replication forks, unwind DNA in the 3'-to-5' direction, and facilitate the loading of the helicase DnaB onto the DNA to restart replication. Single-stranded DNA–binding protein (SSB) is typically present at the abandoned forks, but it is unclear how SSB and PriA interact, although it has been shown that the two proteins interact both physically and functionally. Here, we used atomic force microscopy to visualize the interaction of PriA with DNA substrates with or without SSB. These experiments were done in the absence of ATP to delineate the substrate recognition pattern of PriA before its ATP-catalyzed DNA-unwinding reaction. These analyses revealed that in the absence of SSB, PriA binds preferentially to a fork substrate with a gap in the leading strand. Such a preference has not been observed for 5'- and 3'-tailed duplexes, suggesting that it is the fork structure that plays an essential role in PriA's selection of DNA substrates. Furthermore, we found that in the absence of SSB, PriA binds exclusively to the fork regions of the DNA substrates. In contrast, fork-bound SSB loads PriA onto the duplex DNA arms of forks, suggesting a remodeling of PriA by SSB. We also demonstrate that the remodeling of PriA requires a functional C-terminal domain of SSB. In summary, our atomic force microscopy analyses reveal key details in the interactions between PriA and stalled DNA replication forks with or without SSB.

Tratamento genético foi testado em Layla Richards que estava desenganada pelos médicos. Especialistas ressaltam que os resultados são iniciais e podem não ocorrer em outros pacientes.

The post Terapia inédita reverte leucemia incurável em bebê de 1 ano appeared first on Saúde Próspera.

Wolfgang Dahmen, Felix Gruber and Olga Mula
Math. Comp. 89 (2020), 1605-1646.
Abstract, references and article information

Mays H. Vue
Aug 1, 2011; 24:171-177
Pharmacy and Therapeutics

Abdur Rehman
Nov 1, 2011; 24:234-238
Pharmacy and Therapeutics

Peggy Yarborough
Jan 1, 2003; 16:
Case Studies

Curtis Triplitt
Oct 1, 2006; 19:202-211
Pharmacy Update

Previous studies have shown that sphingosine kinase interacting protein (SKIP) inhibits sphingosine kinase (SK) function in fibroblasts. SK phosphorylates sphingosine producing the potent signaling molecule sphingosine-1-phosphate (S1P). SKIP gene (SPHKAP) expression is silenced by hypermethylation of its promoter in acute myeloid leukemia (AML). However, why SKIP activity is silenced in primary AML cells is unclear. Here, we investigated the consequences of SKIP down-regulation in AML primary cells and the effects of SKIP re-expression in leukemic cell lines. Using targeted ultra-HPLC-tandem MS (UPLC-MS/MS), we measured sphingolipids (including S1P and ceramides) in AML and control cells. Primary AML cells had significantly lower SK activity and intracellular S1P concentrations than control cells, and SKIP-transfected leukemia cell lines exhibited increased SK activity. These findings show that SKIP re-expression enhances SK activity in leukemia cells. Furthermore, other bioactive sphingolipids such as ceramide were also down-regulated in primary AML cells. Of note, SKIP re-expression in leukemia cells increased ceramide levels 2-fold, inactivated the key signaling protein extracellular signal-regulated kinase, and increased apoptosis following serum deprivation or chemotherapy. These results indicate that SKIP down-regulation in AML reduces SK activity and ceramide levels, an effect that ultimately inhibits apoptosis in leukemia cells. The findings of our study contrast with previous results indicating that SKIP inhibits SK function in fibroblasts and therefore challenge the notion that SKIP always inhibits SK activity.

In bacteria, the restart of stalled DNA replication forks requires the DNA helicase PriA. PriA can recognize and remodel abandoned DNA replication forks, unwind DNA in the 3'-to-5' direction, and facilitate the loading of the helicase DnaB onto the DNA to restart replication. Single-stranded DNA–binding protein (SSB) is typically present at the abandoned forks, but it is unclear how SSB and PriA interact, although it has been shown that the two proteins interact both physically and functionally. Here, we used atomic force microscopy to visualize the interaction of PriA with DNA substrates with or without SSB. These experiments were done in the absence of ATP to delineate the substrate recognition pattern of PriA before its ATP-catalyzed DNA-unwinding reaction. These analyses revealed that in the absence of SSB, PriA binds preferentially to a fork substrate with a gap in the leading strand. Such a preference has not been observed for 5'- and 3'-tailed duplexes, suggesting that it is the fork structure that plays an essential role in PriA's selection of DNA substrates. Furthermore, we found that in the absence of SSB, PriA binds exclusively to the fork regions of the DNA substrates. In contrast, fork-bound SSB loads PriA onto the duplex DNA arms of forks, suggesting a remodeling of PriA by SSB. We also demonstrate that the remodeling of PriA requires a functional C-terminal domain of SSB. In summary, our atomic force microscopy analyses reveal key details in the interactions between PriA and stalled DNA replication forks with or without SSB.

(University of Tsukuba) Researchers from the University of Tsukuba discovered that fish schools showed a significant change in behavior with varying school sizes. Using integrated information theory, they showed that a significant change in the interaction between the fish and the overall collective behavior occurred between three- and four-fish schools, including the emergence of leadership within the group. These findings help understand the dynamics of collective behavior.