La gobernadora del Chocó le solicitó a la mesa de negociación que las comunidades del San Juan sean incluidas en el diálogo con el ELN.

Agencia de seguridad nacional y desafíos de la ciberseguridad

En este
episodio, La Luciérnaga se enciende para hablar de las investigaciones por
presuntas amenazas contra Gustavo Petro. También, se aplaza el debate de
control político contra el ministro de Defensa, Diego Molano. Finalmente,
Corozo se pega una vuelta por el programa, mientras sigue triunfando en
Masterchef, para hablar del alza en los precios de la canasta básica.   



La Luciérnaga un espacio de humor, análisis y opinión de Caracol
Radio que acompaña hace 30 años a sus oyentes en el regreso a casa. 

Escuche el programa de este miércoles 21agosto La Luciérnaga, un espacio de humor y opinión de Caracol Radio que desde hace 31 años acompaña a sus oyentes en su regreso casa.

Jorge Wilson Blanco Rincón, personero delegado para Seguridad, Convivencia y Justicia explica el informe en el que se evidencian cuales son las zonas donde más hay hurtos y homicidios.

La joven llamó a su padre el sábado tras notar que corría riesgo por los ataques del grupo armado Hamas a Israel.

Paulina Rubio estará en Bogotá y Medellín este viernes 16 y sábado 17 de febrero.

Juan Carlos Pereira se ganó la titularidad de Millonarios a pulso, el volante cartagenero ha sido pieza clave en el equipo de Alberto Gamero durante los últimos partidos, donde ha sido incluso destacado como figura del equipo. En diálogo con El Alargue de Caracol Radio, Pereira contó cómo ha ido recuperando su nivel, habló del camerino, del proceso de Gamero y de su amistad con Beckham David Castro, entre otras cosas.

En 6AM Hoy por Hoy de Caracol Radio estuvo Henry Cárdenas, presidente de Fedetranscarga, para hablar sobre Cuáles son las posibilidades de que levanten los bloqueos.

Francisco de Roux se refirió en 6AM a los señalamientos de un padre de familia, quien lo acusa de presunto encubrimiento en un caso de abuso en la iglesia en la década de 1970.

Today on Q with Tom Power: music producer Rick Rubin and cartoonist Barbara Brandon-Croft

President-elect Donald Trump is considering tapping Sen. Marco Rubio (R-FL) as secretary of state, according to the New York Times.

The post Report: President-Elect Donald Trump Considering Marco Rubio as Secretary of State appeared first on Breitbart.

RUBiS Energy Bermuda has shared details regarding the opening hours for their various locations during the Christmas and New Year’s holiday season. Related Stories RUBiS Christmas & New Year’s Holiday Hours RUBiS Service Station Christmas Holiday Hours Govt Responds To Comments On NSC Contract Gas Stations: Opening Hours Over The Holiday Oil Insurance Upgraded By […]

1. Beijing and Washington stops provide clues for Indonesia's direction under Prabowo Subianto  ABC News
2. Prabowo pledges co-operation with Trump  The Australian Financial Review
3. Indonesian president meets Biden and speaks with Trump  The Canberra Times
4. At White House, Indonesia's new leader straddles US-China rivalry  VOA Asia
5. Will Prabowo Subianto cosy up to Donald Trump or to China?  The Economist

Forget that occasionally sane Marco Rubio we've caught glimpses of, he will completely fold to Donald Trump as Secretary of State. Do we at least get closer to a Senate majority with his leaving? No, we do not.

Florida Man DeSantis gets to appoint Rubio's temporary Senate replacement. The seat doesn't open until 2028.

Whoever's appointed would serve until a 2026 special election to complete the final two years of Rubio's term - so whoever's appointed has to win again in 2028.

First off the bat, Puddin' Fingers could appoint his wife, Casey "I'm Really Jackie Kennedy" DeSantis, to hold the seat until his term as governor is up.

He could also appoint his lieutenant governor, Jeanette Nunez. That would leave the option of allowing her to appoint him to the Senate seat.

According to CBS News, Attorney General Ashley Moody and former Florida House Speaker Jose Oliva are also in the mix.

Rubik's Cube Club

Trump could name Rubio as his secretary of state and Noem as his homeland security secretary, US media say.

It has been a night of contrasting emotions as vote-counting continues across key swing states.

1. Trump taps Marco Rubio & Michael Waltz, known to be pro-India and tough on China, for top jobs  The Indian Express
2. Donald Trump Sets 'Existential' Battle Against China With Cabinet Picks  NDTV
3. Trump's NSA pick Mike Waltz's pro-India stand in 5 quotes  Firstpost
4. Trump's incoming NSA Mike Waltz wants US to dance cheek-to-check with India  The Times of India
5. Who is Mike Waltz, Donald Trump’s pick for national security adviser?  The Hindu

Interactive Media Strategies released data on July 14 showing that 62% of corporations that purchased video communications technology made the decision at the President/CEO level when there was no prior investment in this kind of technology.  That number dropped to 58% when the prior year's spend was as much as $10,000.

The same data shows that when an enterprise already spends $100,000 or more annually on video, the decision-making authority is almost evenly distributed between IT (35%), functional department heads (31%), and the President/CEO level (34%).

I think the clear implication of this data is that when corporations are already committing resources to video communications and the value of video has already been established, and this kind of technology is no longer considered exotic, then decision-making authority becomes more broadly distributed to IT and the business units.

This data is interesting when paired with data released in 2010 that shows 15% of executives surveyed that do not spend money on video and are thus not using video communications believe video communications are "very effective."  58% of executives surveyed that spend $100,000 or more annual on video technology indicated they believe video communications are "very effective."

I am sure this great difference is due to several factors, including: executives who are predisposed to see value in video are those most likely to invest in it, and those that have already made a six figure investment in video will likely not be motivated to feel as if the investment was wasted.  Nonetheless, video obviously wears well because executives who are heavily invested in video believe much more in its value than executives who do not employ the technology.

President-elect Donald Trump is expected to nominate Florida Sen. Marco Rubio as his secretary of state, reports late Monday indicate. According to The Wall Street Journal, while the nomination isn’t […]

The post Trump to Pick Senator Marco Rubio for Crucial Cabinet Position: Report appeared first on The Western Journal.

The QT interval is a recording of cardiac electrical activity. Previous genome-wide association studies identified genetic variants that modify the QT interval upstream of LITAF (lipopolysaccharide-induced tumor necrosis factor-α factor), a protein encoding a regulator of endosomal trafficking. However, it was not clear how LITAF might impact cardiac excitation. We investigated the effect of LITAF on the voltage-gated sodium channel Nav1.5, which is critical for cardiac depolarization. We show that overexpressed LITAF resulted in a significant increase in the density of Nav1.5-generated voltage-gated sodium current INa and Nav1.5 surface protein levels in rabbit cardiomyocytes and in HEK cells stably expressing Nav1.5. Proximity ligation assays showed co-localization of endogenous LITAF and Nav1.5 in cardiomyocytes, whereas co-immunoprecipitations confirmed they are in the same complex when overexpressed in HEK cells. In vitro data suggest that LITAF interacts with the ubiquitin ligase NEDD4-2, a regulator of Nav1.5. LITAF overexpression down-regulated NEDD4-2 in cardiomyocytes and HEK cells. In HEK cells, LITAF increased ubiquitination and proteasomal degradation of co-expressed NEDD4-2 and significantly blunted the negative effect of NEDD4-2 on INa. We conclude that LITAF controls cardiac excitability by promoting degradation of NEDD4-2, which is essential for removal of surface Nav1.5. LITAF-knockout zebrafish showed increased variation in and a nonsignificant 15% prolongation of action potential duration. Computer simulations using a rabbit-cardiomyocyte model demonstrated that changes in Ca2+ and Na+ homeostasis are responsible for the surprisingly modest action potential duration shortening. These computational data thus corroborate findings from several genome-wide association studies that associated LITAF with QT interval variation.

Ivan Matic
Jan 1, 2008; 7:132-144
Research

Tamar Geiger
Mar 1, 2012; 11:M111.014050-M111.014050
Special Issue: Prospects in Space and Time

Sebastian A. Wagner
Oct 1, 2011; 10:M111.013284-M111.013284
Research

Christian Tagwerker
Apr 1, 2006; 5:737-748
Research

The Platinum Jubilee of Her Majesty Queen Elizabeth II News release NCapeling 1 June 2022

The staff, associate fellows and Council of the Royal Institute of International Affairs at Chatham House send congratulations and warmest wishes to Her Majesty Queen Elizabeth II on the occasion of her momentous Platinum Jubilee.

As the Patron of the institute since her accession to the throne in 1952, HM The Queen has underpinned Chatham House’s independence for seven decades and thereby strengthened the impact of our work on the critical issues facing the world.

HM The Queen lent her personal support to the establishment of the annual Chatham House Prize in 2005 and has presented the award in person on behalf of the institute’s members on three occasions.

Among her other direct engagements with Chatham House, HM The Queen has helped us engage the next generation by supporting and then attending the launch of the Queen Elizabeth II Academy for Leadership in International Affairs in 2014, when she met the first intake of Academy fellows.

We are enormously grateful for her continued involvement as Patron of the institute and wish her and the Royal Family a memorable Platinum Jubilee.

DL Brasaemle
Nov 1, 1997; 38:2249-2263
Articles

Ryan J Lumpkin
Dec 2, 2020; 0:RA120.002414v1-mcp.RA120.002414
Research

The glucagon receptor (GCGR) activated by the peptide hormone glucagon is a seven-transmembrane G protein–coupled receptor (GPCR) that regulates blood glucose levels. Ubiquitination influences trafficking and signaling of many GPCRs, but its characterization for the GCGR is lacking. Using endocytic colocalization and ubiquitination assays, we have identified a correlation between the ubiquitination profile and recycling of the GCGR. Our experiments revealed that GCGRs are constitutively ubiquitinated at the cell surface. Glucagon stimulation not only promoted GCGR endocytic trafficking through Rab5a early endosomes and Rab4a recycling endosomes, but also induced rapid deubiquitination of GCGRs. Inhibiting GCGR internalization or disrupting endocytic trafficking prevented agonist-induced deubiquitination of the GCGR. Furthermore, a Rab4a dominant negative (DN) that blocks trafficking at recycling endosomes enabled GCGR deubiquitination, whereas a Rab5a DN that blocks trafficking at early endosomes eliminated agonist-induced GCGR deubiquitination. By down-regulating candidate deubiquitinases that are either linked with GPCR trafficking or localized on endosomes, we identified signal-transducing adaptor molecule–binding protein (STAMBP) and ubiquitin-specific protease 33 (USP33) as cognate deubiquitinases for the GCGR. Our data suggest that USP33 constitutively deubiquitinates the GCGR, whereas both STAMBP and USP33 deubiquitinate agonist-activated GCGRs at early endosomes. A mutant GCGR with all five intracellular lysines altered to arginines remains deubiquitinated and shows augmented trafficking to Rab4a recycling endosomes compared with the WT, thus affirming the role of deubiquitination in GCGR recycling. We conclude that the GCGRs are rapidly deubiquitinated after agonist-activation to facilitate Rab4a-dependent recycling and that USP33 and STAMBP activities are critical for the endocytic recycling of the GCGR.

Genetic mutations related to ALS, a progressive neurological disease, have been discovered in the gene encoding σ-1 receptor (σ1R). We previously reported that σ1RE102Q elicits toxicity in cells. The σ1R forms oligomeric states that are regulated by ligands. Nevertheless, little is known about the effect of ALS-related mutations on oligomer formation. Here, we transfected NSC-34 cells, a motor neuronal cell line, and HEK293T cells with σ1R-mCherry (mCh), σ1RE102Q-mCh, or nontagged forms to investigate detergent solubility and subcellular distribution using immunocytochemistry and fluorescence recovery after photobleaching. The oligomeric state was determined using crosslinking procedure. σ1Rs were soluble to detergents, whereas the mutants accumulated in the insoluble fraction. Within the soluble fraction, peak distribution of mutants appeared in higher sucrose density fractions. Mutants formed intracellular aggregates that were co-stained with p62, ubiquitin, and phosphorylated pancreatic eukaryotic translation initiation factor-2-α kinase in NSC-34 cells but not in HEK293T cells. The aggregates had significantly lower recovery in fluorescence recovery after photobleaching. Acute treatment with σ1R agonist SA4503 failed to improve recovery, whereas prolonged treatment for 48 h significantly decreased σ1RE102Q-mCh insolubility and inhibited apoptosis. Whereas σ1R-mCh formed monomers and dimers, σ1RE102Q-mCh also formed trimers and tetramers. SA4503 reduced accumulation of the four types in the insoluble fraction and increased monomers in the soluble fraction. The σ1RE102Q insolubility was diminished by σ1R-mCh co-expression. These results suggest that the agonist and WT σ1R modify the detergent insolubility, toxicity, and oligomeric state of σ1RE102Q, which may lead to promising new treatments for σ1R-related ALS.