WASHINGTON: A large majority of Americans oppose reopening the country now, with Democrats and Republicans sharply divided on the question, according to a poll published on Friday.Sixty-four percent of those polled by ABC News/Ipsos said opening the country now is not worth it because it will mean...

ISLAMABAD: Minister for Information and Broadcasting Senator Shibli Faraz on Friday said people's cooperation is vital for the success of lifting the restrictions of lockdown. In a tweet, he said Standard Operating Procedures for various businesses will have to be implemented strictly. He said...

ISLAMABAD: Minister for Information and Broadcasting Senator Shibli Faraz on Friday said people's cooperation is vital for the success of lifting the restrictions of lockdown.In a tweet, he said Standard Operating Procedures for various businesses will have to be implemented strictly.He said that...

ISLAMABAD: Minister for Information and Broadcasting Senator Shibli Faraz on Friday said people's cooperation is vital for the success of lifting the restrictions of lockdown.In a tweet, he said Standard Operating Procedures for various businesses will have to be implemented strictly.He said that...

LONDON: Fifty Labour Party parliamentarians have told the new Labour leader, Sir Keir Starmer, that his recent statement on Indian-occupied Kashmir after a meeting with the Labour Friends of India is unacceptable, illegal, and historically wrong and he must clarify his position or face open...

The Ministry of Environment, Forest and Climate Change (MoEFCC) has drafted the Environment Impact Assessment (EIA) notification 2020 to curb the construction violations across the country. The new draft would replace the EIA notification 2006. The Ministry of Environment, Forest and Climate change (MoEFCC) has proposed a new set of environment clearance norms in its Environment Impact Assessment (EIA) notification 2020. Earlier, the environment approvals to construction projects  were given as per the criteria stated in the EIA notification 2006; however, the new draft would replace the former set of rules. The Ministry has placed the EIA report in the public domain on March 12, 2020 and has sought views and comments […]

The Sindh revenue department has written a letter to Chief Minister Sindh Syed Murad Ali Shah for sanctioning extra incentives to the officials and officers of different departments participating in the battle against COVID-19.The letter, which was sent to the CM on Friday, reads that as the...

dsPIC33CK64MC105 General Purpose Plug-In Module (PIM) Information Sheet

Kuwait will enact a "total curfew" from 4pm (1300 GMT) Sunday through to May 30 to help to curb the spread of the new coronavirus, the Information Ministry said on Twitter Friday.

LOS ANGELES, CALIF. – "The Distortion of Sound,” a documentary exposing the decline of sound and how technology has changed the way we listen to music, premieres this evening at an invitation-only event at the GRAMMY® Museum in Los Angeles, California. Following tonight’s screening, the documentary will air on “The Distortion of Sound” YouTube channel, DistortionofSound.com, the Sundance Channel the IFC Channel on July 23 at 6 p.m. ET and PT.*

Jane Goodall has tirelessly fought for a better world for humans and wildlife, and with covid-19 we must stay positive, she says 

The largest union representing U.S. meatpacking workers said on Friday it opposed the reopening of plants as the Trump administration had failed to guarantee workers' safety.

Elisabeth Bik is on a mission to detect duplicate images in scientific papers, exposing either genuine mistakes or signs of fraud. But her work isn't always appreciated, she says

Is toilet squatting really better than just sitting, or are the supposed benefits of a squatty potty just the fantasy of a rainbow-pooping unicorn?

The largest union representing U.S. meatpacking workers said on Friday it opposed the reopening of plants as the Trump administration had failed to guarantee workers' safety.

Title: Blind Cords Pose Danger to Toddlers, Doctors Warn
Category: Health News
Created: 4/30/2013 10:35:00 AM
Last Editorial Review: 4/30/2013 12:00:00 AM

Title: Sharing Breast Milk May Pose Risks Women Haven't Considered
Category: Health News
Created: 4/30/2015 12:00:00 AM
Last Editorial Review: 5/1/2015 12:00:00 AM

Title: FDA Proposes Ban on 'Shock' Device Used to Curb Self-Harm
Category: Health News
Created: 4/22/2016 12:00:00 AM
Last Editorial Review: 4/25/2016 12:00:00 AM

Title: Proposed Cuts in U.S. Food and Health Aid Would Hurt Families
Category: Health News
Created: 3/13/2020 12:00:00 AM
Last Editorial Review: 3/16/2020 12:00:00 AM

Title: Modern Livestock Farming Can Pose Public Health Risk
Category: Health News
Created: 5/7/2020 12:00:00 AM
Last Editorial Review: 5/8/2020 12:00:00 AM

Title: 'Couch Potato' Lifestyle Poses Danger to Women's Hearts
Category: Health News
Created: 2/18/2020 12:00:00 AM
Last Editorial Review: 2/19/2020 12:00:00 AM

ABSTRACT

In the absence of a vaccine, multidrug-resistant Neisseria gonorrhoeae has emerged as a major human health threat, and new approaches to treat gonorrhea are urgently needed. N. gonorrhoeae pili are posttranslationally modified by a glycan that terminates in a galactose. The terminal galactose is critical for initial contact with the human cervical mucosa via an interaction with the I-domain of complement receptor 3 (CR3). We have now identified the I-domain galactose-binding epitope and characterized its galactose-specific lectin activity. Using surface plasmon resonance and cellular infection assays, we found that a peptide mimic of this galactose-binding region competitively inhibited the N. gonorrhoeae-CR3 interaction. A compound library was screened for potential drugs that could similarly prohibit the N. gonorrhoeae-CR3 interaction and be repurposed as novel host-targeted therapeutics for multidrug-resistant gonococcal infections in women. Two drugs, methyldopa and carbamazepine, prevented and cured cervical cell infection by multidrug-resistant gonococci by blocking the gonococcal-CR3 I-domain interaction.

IMPORTANCE Novel therapies that avert the problem of Neisseria gonorrhoeae with acquired antibiotic resistance are urgently needed. Gonococcal infection of the human cervix is initiated by an interaction between a galactose modification made to its surface appendages, pili, and the I-domain region of (host) complement receptor 3 (CR3). By targeting this crucial gonococcal–I-domain interaction, it may be possible to prevent cervical infection in females. To this end, we identified the I-domain galactose-binding epitope of CR3 and characterized its galactose lectin activity. Moreover, we identified two drugs, carbamazepine and methyldopa, as effective host-targeted therapies for gonorrhea treatment. At doses below those currently used for their respective existing indications, both carbamazepine and methyldopa were more effective than ceftriaxone in curing cervical infection ex vivo. This host-targeted approach would not be subject to N. gonorrhoeae drug resistance mechanisms. Thus, our data suggest a long-term solution to the growing problem of multidrug-resistant N. gonorrhoeae infections.

ABSTRACT

Environmental exposure has a significant impact on human health. While some airborne fungi can cause life-threatening infections, the impact of environment on fungal spore dispersal and transmission is poorly understood. The democratization of shotgun metagenomics allows us to explore important questions about fungal propagation. We focus on Pneumocystis, a genus of host-specific fungi that infect mammals via airborne particles. In humans, Pneumocystis jirovecii causes lethal infections in immunocompromised patients if untreated, although its environmental reservoir and transmission route remain unclear. Here, we attempt to clarify, by analyzing human exposome metagenomic data sets, whether humans are exposed to different Pneumocystis species present in the air but only P. jirovecii cells are able to replicate or whether they are selectively exposed to P. jirovecii. Our analysis supports the latter hypothesis, which is consistent with a local transmission model. These data also suggest that healthy carriers are a major driver for the transmission.

The entry/fusion complex (EFC) consists of 11 conserved proteins embedded in the membrane envelope of mature poxvirus particles. Poxviruses also encode proteins that localize in cell membranes and negatively regulate superinfection and syncytium formation. The vaccinia virus (VACV) A56/K2 fusion regulatory complex associates with the G9/A16 EFC subcomplex, but functional support for the importance of this interaction was lacking. Here, we describe serially passaging VACV in nonpermissive cells expressing A56/K2 as an unbiased approach to isolate and analyze escape mutants. Viruses forming large plaques in A56/K2 cells increased in successive rounds of infection, indicating the occurrence and enrichment of adaptive mutations. Sequencing of genomes of passaged and cloned viruses revealed mutations near the N terminus of the G9 open reading frame but none in A16 or other genes. The most frequent mutation was His to Tyr at amino acid 44; additional escape mutants had a His-to-Arg mutation at amino acid 44 or a duplication of amino acids 26 to 39. An adaptive Tyr-to-Cys substitution at amino acid 42 was discovered using error-prone PCR to generate additional mutations. Myristoylation of G9 was unaffected by the near-N-terminal mutations. The roles of the G9 mutations in enhancing plaque size were validated by homologous recombination. The mutants exhibited enhanced entry and spread in A56/K2 cells and induced syncytia at neutral pH in HeLa cells despite the expression of A56/K2. The data suggest that the mutations perturb the interaction of G9 with A56/K2, although some association was still detected in detergent-treated infected cell lysates.

IMPORTANCE The entry of enveloped viruses is achieved by the fusion of viral and cellular membranes, a critical step in infection that determines host range and provides targets for vaccines and therapeutics. Poxviruses encode an exceptionally large number of proteins comprising the entry/fusion complex (EFC), which enables infection of diverse cells. Vaccinia virus (VACV), the prototype member of the poxvirus family, also encodes the fusion regulatory proteins A56 and K2, which are displayed on the plasma membrane and may be beneficial by preventing reinfection and cell-cell fusion. Previous studies showed that A56/K2 interacts with the G9/A16 EFC subcomplex in detergent-treated cell extracts. Functional evidence for the importance of this interaction was obtained by serially passaging wild-type VACV in cells that are nonpermissive because of A56/K2 expression. VACV mutants with amino acid substitutions or duplications near the N terminus of G9 were enriched because of their ability to overcome the block to entry imposed by A56/K2.

Decreased release of palmitic acid methyl ester (PAME), a vasodilator, from perivascular adipose tissue (PVAT) might contribute to hypertension pathogenesis. However, the PAME biosynthetic pathway remains unclear. In this study, we hypothesized that PAME is biosynthesized from palmitic acid (PA) via human catechol-O-methyltransferase (COMT) catalysis and that decreased PAME biosynthesis plays a role in hypertension pathogenesis. We compared PAME biosynthesis between age-matched normotensive Wistar Kyoto (WKY) rats and hypertensive spontaneously hypertensive rats (SHRs) and investigated the effects of losartan treatment on PAME biosynthesis. Computational molecular modeling indicated that PA binds well at the active site of COMT. Furthermore, in in vitro enzymatic assays in the presence of COMT and S-5'-adenosyl-L-methionine (AdoMet), the stable isotope [¹³C₁₆]-PA was methylated to form [¹³C₁₆]-PAME in incubation medium or the Krebs–Henseleit solution containing 3T3-L1 adipocytes or rat PVAT. The adipocytes and PVATs expressed membrane-bound (MB)-COMT and soluble (S)-COMT proteins. [¹³C₁₆]-PA methylation to form [¹³C₁₆]-PAME in 3T3-L1 adipocytes and rat PVAT was blocked by various COMT inhibitors, such as S-(5'-adenosyl)-L-homocysteine, adenosine-2',3'-dialdehyde, and tolcapone. MB- and S-COMT levels in PVATs of established SHRs were significantly lower than those in PVATs of age-matched normotensive WKY rats, with decreased [¹³C₁₆]-PA methylation to form [¹³C₁₆]-PAME. This decrease was reversed by losartan, an angiotensin II (Ang II) type 1 receptor antagonist. Therefore, PAME biosynthesis in rat PVAT is dependent on AdoMet, catalyzed by COMT, and decreased in SHRs, further supporting the role of PVAT/PAME in hypertension pathogenesis. Moreover, the antihypertensive effect of losartan might be due partly to its increased PAME biosynthesis.

Viruses and mobile genetic elements are molecular parasites or symbionts that coevolve with nearly all forms of cellular life. The route of virus replication and protein expression is determined by the viral genome type. Comparison of these routes led to the classification of viruses into seven "Baltimore classes" (BCs) that define the major features of virus reproduction. However, recent phylogenomic studies identified multiple evolutionary connections among viruses within each of the BCs as well as between different classes. Due to the modular organization of virus genomes, these relationships defy simple representation as lines of descent but rather form complex networks. Phylogenetic analyses of virus hallmark genes combined with analyses of gene-sharing networks show that replication modules of five BCs (three classes of RNA viruses and two classes of reverse-transcribing viruses) evolved from a common ancestor that encoded an RNA-directed RNA polymerase or a reverse transcriptase. Bona fide viruses evolved from this ancestor on multiple, independent occasions via the recruitment of distinct cellular proteins as capsid subunits and other structural components of virions. The single-stranded DNA (ssDNA) viruses are a polyphyletic class, with different groups evolving by recombination between rolling-circle-replicating plasmids, which contributed the replication protein, and positive-sense RNA viruses, which contributed the capsid protein. The double-stranded DNA (dsDNA) viruses are distributed among several large monophyletic groups and arose via the combination of distinct structural modules with equally diverse replication modules. Phylogenomic analyses reveal the finer structure of evolutionary connections among RNA viruses and reverse-transcribing viruses, ssDNA viruses, and large subsets of dsDNA viruses. Taken together, these analyses allow us to outline the global organization of the virus world. Here, we describe the key aspects of this organization and propose a comprehensive hierarchical taxonomy of viruses.

An aging global population combined with sedentary lifestyles and unhealthy diets has contributed to an increasing incidence of obesity and type 2 diabetes. These metabolic disorders are associated with perturbations to nitric oxide (NO) signaling and impaired glucose metabolism. Dietary inorganic nitrate, found in high concentration in green leafy vegetables, can be converted to NO in vivo and demonstrates antidiabetic and antiobesity properties in rodents. Alongside tissues including skeletal muscle and liver, white adipose tissue is also an important physiological site of glucose disposal. However, the distinct molecular mechanisms governing the effect of nitrate on adipose tissue glucose metabolism and the contribution of this tissue to the glucose-tolerant phenotype remain to be determined. Using a metabolomic and stable-isotope labeling approach, combined with transcriptional analysis, we found that nitrate increases glucose uptake and oxidative catabolism in primary adipocytes and white adipose tissue of nitrate-treated rats. Mechanistically, we determined that nitrate induces these phenotypic changes in primary adipocytes through the xanthine oxidoreductase–catalyzed reduction of nitrate to NO and independently of peroxisome proliferator–activated receptor-α. The nitrate-mediated enhancement of glucose uptake and catabolism in white adipose tissue may be a key contributor to the antidiabetic effects of this anion.

Recently, there has been a worldwide resurgence in pneumoconiosis, or pulmonary fibrosis due to occupational mineral dust exposure. In Queensland, Australia, there has been a re-emergence of coal workers' pneumoconiosis and silicosis. Some coal mining communities have experienced a resurgence of progressive massive fibrosis in the USA and a worldwide epidemic is occurring of accelerated silicosis due to exposure to artificial stone.

These diseases are all preventable and should not be occurring in the 21st century. Best practice prevention includes reduction of exposure to mineral dusts or, ideally, prevention of exposure altogether. However, where dust exposure has occurred, respiratory surveillance can provide a strategy for early disease detection. It is important to identify early signs of occupational lung disease at a stage where intervention may be beneficial, though it must be acknowledged that progression may occur even after cessation of exposure to dusts. Respiratory surveillance should be distinguished from population screening and case finding, which are different methods used for disease investigation and control. Designing an ideal respiratory surveillance programme is challenging, as there is no single test that accurately identifies early disease. Several different respiratory disorders may occur related to the same exposure(s). Physicians organising and interpreting tests used in respiratory surveillance must be aware of the broad range of potential work-related respiratory conditions, complexities in diagnosis, and appropriate interpretation of the exposure history, as well as current management options. A working knowledge of the compensation and medicolegal avenues available to workers in individual jurisdictions is also useful.

MERVYN KING, the former governor of the Bank of England, once issued a brutal analysis of the global banking system and argued for its reinvention, it can be revealed as the Government fine-tunes its economic response to the coronavirus pandemic.

Environmental biologists have warned that the potential spread of COVID-19 via sewage 'must not be neglected' in the battle to protect human health.

They come from some of the most prominent hockey families in Manitoba, a group of young men blessed with athletic gifts that allow them to be better than most at ...

What film critics have to say about this week's new releases.

THE leader of the Scottish Conservative Party has outlined his plan to stop Nicola Sturgeon and the SNP becoming Scotland's biggest party at the 2021 Scottish election.

Joseph R. Biden said Friday President Trump's ham-handed response to the coronavirus has helped to steer the nation into a Great Depression-like economic tailspin.

Mr. Biden said Mr. Trump "utterly failed" to prepare for COVID-19 and said the virus has "laid bare" the damage Mr. Trump has done by pursuing ...

Environmental campaigners against electric plant and pipeline say Samir Flores Soberanes’s murder is a ‘political crime’

A Mexican environmental activist has been murdered before a referendum on a controversial thermal-electric plant and pipeline that he opposed.

Samir Flores Soberanes, an indigenous Náhuatl, was killed in his home during the early hours of Wednesday in the town of Amilcingo in Morelos state, 80 miles south of Mexico City. He was a human rights activist, producer for a community radio station and long-time opponent of the Proyecto Integral Morelos (the integral project for Morelos) – which includes the plant and pipeline.

Here’s what notifications for iOS and Android COVID-19 tracing will look like.

MPs will investigate the potential consequences of allowing electric scooters to be used on the road.

Two men have been charged after a £3 million cocaine haul was uncovered in a purpose-built smuggling lorry in Dover.

Labour has proposed legislation to deliver a dedicated fast-tracked fund for domestic abuse services affected by the coronavirus crisis.

The Duchess of Cornwall has revealed she has taken up ballet and is practising her poses and positions to keep fit in lockdown.

Coronavirus: the symptoms Read our LIVE updates on the coronavirus here

A homeowner was conned out of more than £100,000 by rogue builders who overcharged for roof repairs — before posing as police and trading standards officers to swindle him again.

Most Brits are against ending the coronavirus lockdown this week, with less than one in five thinking the time is right to reopen pubs, restaurants, stadiums and schools, a new survey shows.

The travel industry has sifted through the BBC show’s many sex scenes to showcase shots of Ireland’s landscape

Promoting Ireland as a tourism destination used to be straightforward – just showcase the bucolic landscape and put a slogan on the end – but that was before Normal People turned a chunk of the Atlantic coast into Fifty Shades of Sligo.

The television adaptation of Sally Rooney’s novel features beautiful shots of Sligo’s beaches and mountains, plus Trinity College Dublin, but there is also sex. Lots of sex.

As coronavirus-induced economic pain grew around the world, some U.S. states moved forward Friday with plans to reopen their economies, while European countries held muted commemorations to mark the 75th anniversary of the end of World War II on the continent. Around the U.K., residents were encouraged to stand on their doorsteps and sing together while socially distanced to commemorate the Allies' victory over Nazi Germany. Meanwhile, South Korea, seen as a model for containing COVID-19, ordered nightclubs closed for a month and considered delaying a school restart after an uptick in coronavirus cases.

Uncooked pet food could be source of pathogens dangerous to humans, research suggests

First and last names, email addresses, gender and sexual orientation, and credit card information of models and users was left on an insecure server