During the restorations, visitors will be able to see the famous site via a temporary walkway, which officials will use to study the flow of foot traffic

Scientists in France solved the evolutionary mystery of this prehistoric monster, which resembles both the centipedes and millipedes of today

Once one of the world’s most dangerous border crossings, Berlin's symbol of death and division has been turned into a tangible way to experience history

Laboratory study of natural populations of mosquitoes can play a key role in determining the underlying causes of variation in burdens of mosquito-borne disease. Aedes aegypti is the main vector of the viruses that cause dengue, chikungunya, Zika, and yellow fever, making it a high priority for laboratory study. Ae. aegypti eggs provide an ideal starting point for new laboratory colonies. Eggs can be collected using ovicups, which are small plastic cups lined with seed-germination paper and partially filled with leaf-infused H₂O. Once collected, dry eggs will remain viable for months and can be safely transported long distances back to the laboratory as long as they are properly stored. This protocol provides step-by-step instructions for preparing for collecting, storing, and hatching Ae. aegypti eggs and has successfully yielded laboratory colonies from locations across both the native and invasive range of this species.

Creating transgenic mosquitoes allows for mechanistic studies of basic mosquito biology and the development of novel vector control strategies. CRISPR–Cas9 gene editing has revolutionized gene editing, including in mosquitoes. This protocol details part of the gene editing process of Aedes aegypti mosquitoes via CRISPR–Cas9, through testing and validating single-guide RNAs (sgRNAs). Gene editing activity varies depending on the sequence of sgRNAs used, so validation of sgRNA activity should be done before large-scale generation of mutants or transgenics. sgRNA is designed using online tools and synthesized in <1 h. Once mutants or transgenics are generated via embryo microinjection, sgRNA activity is validated by quick genotyping polymerase chain reaction (PCR) and DNA sequencing.

The insect eggshell is a multifunctional structure with several important roles, including generating an entry point for sperm via the micropyle before oviposition, serving as an oviposition substrate attachment surface, and functioning as a protective layer during embryo development. Eggshell proteins play major roles in eggshell tanning and hardening following oviposition and provide molecular cues that define dorsal–ventral axis formation. Precise eggshell formation during ovarian follicle maturation is critical for normal embryo development and the synthesis of a defective eggshell often gives rise to inviable embryos. Therefore, simple and accurate methods for identifying eggshell proteins will facilitate our understanding of the molecular pathways regulating eggshell formation and the mechanisms underlying normal embryo development. This protocol describes how to isolate and enrich eggshells from mature oocytes of Aedes aegypti mosquitoes and how to extract their eggshell proteins for liquid chromatography with tandem mass spectrometry (LC–MS/MS) proteomic analysis. Although this methodology was developed for studying mosquito eggshells, it may be applicable to eggs from a variety of insects. Mosquitoes are ideal model organisms for this study as their ovarian follicle development and eggshell formation are meticulously regulated by blood feeding and their follicles develop synchronously throughout oogenesis in a time-dependent manner.

In insects, oocyte resorption (oosorption) or follicular atresia is one of the key physiological processes and evolutionary strategies used to optimize reproductive fitness. Mosquitoes are ideal model organisms for studying egg maturation in arthropods, as their follicle development is initiated only following the ingestion of a blood meal, followed by a carefully orchestrated series of hormonally regulated events leading to egg maturation. A cohort of approximately 100 follicles per mosquito ovary begin developing synchronously. However, a significant fraction of follicles ultimately undergo apoptosis and oosorption, especially when available resources from the blood meal are limited. Therefore, simple, rapid, and reliable techniques to accurately evaluate follicular atresia are necessary to understand mechanisms underlying follicle development in insects. This protocol describes how to detect apoptotic follicle cells within the Aedes aegypti mosquito ovaries using a commercially available fluorescent-labeled inhibitor of caspases (FLICA). Caspases are key players in animal apoptosis. In this assay, the FLICA reagent enters the intracellular compartment of follicles in dissected mosquito ovaries and covalently binds to active caspases. The bound reagent remains within the cell and its fluorescent signal can be observed by confocal microscopy. Although this method was specifically developed for visualizing apoptotic ovarian follicles during Ae. aegypti mosquito egg development, it should be applicable to other mosquito tissues that undergo caspase-mediated program cell death in a time-dependent manner.

Buenos Aires, Argentinen :: Technische Schiffsmitarbeiter geben Gottes Liebe an Schweißer vor Ort weiter, die bei der Reparatur der Logos Hope helfene

The entrepreneur and financial adviser defeated Adam Bassett for the party nomination in Egmont after two days of voting last week. 

A federal audit of Canada’s strategy to promote the extraction of critical minerals says the government isn’t giving due consideration to the potential impact of mining on the environment, biodiversity and Indigenous people.

The Toronto Police Services Board unanimously approved a new five-year hiring plan for the force on Tuesday.

Swedish knife manufacturer reduces new product design time and costs

Apple's 2024 Mac mini stashes the power button underneath, a bold move that executives say aligns with user habits, even if it's left some fans scratching their heads.

OM Sweden's newest team members - a van and a trailer - bring new opportunities for sharing and recruiting.

OM Sweden hosts its first outreach in 17 years.

OM Sweden plans church-planting initiatives in three urban centres.

OM Sweden joined OM Ireland for outreach over the Easter holiday in Carlow town.

One widow in Pakistan finds herself supporting her family with no visible means of help; until she gains help from the OM tailoring skills course.

The change to the Title II program will benefit Southern states, while Michigan, New York, and Pennsylvania, among others, will see their allocations shrink.

On Thursday, Senate Democratic Whip Dick Durbin (D-Ill.) introduced legislation that would establish a national standard for youth-concussion treatment and prevention.

A report suggests that the $2.5 billion program should focus more on continuous improvement than on scattershot activities.

The largest experiment to date comparing commercial math curricula gives a slight edge to two popular programs.

New sheets outlines how districts can support English-learner students, but concedes that "schools may not be able to provide all services in the same manner they are typically provided."

The Certified Family Life Educator credential, available through the Human Development and Family Studies degree program, was recently endorsed by the U.S. Department of Veterans Affairs and the Office of Head Start.

U.S. Supreme Court nominee Amy Coney Barrett said it would be "unthinkable" for the landmark "Brown" desegregation decision to be overruled.

Disconnections make it tough for homeless students to stay in school, says a new study, which also details the new requirements in the Every Student Succeeds Act that bolster resources for their support.

Since June 2020 Education Week has tracked if & when the over 200 schools named after men with ties to the Confederacy changed their names.

An overview of the biggest business developments in SA and beyond.

Here are five takeaways on the teaching profession from the newly released 2017-18 National Teacher and Principal Survey.

Final Federal Assistance Is Available Through Early 2024; State-Funded Assistance Is Still Available and Ongoing   Dover, Del., September 18, 2023 – As part of the state’s ongoing response to the COVID-19 public health emergency, the Delaware State Housing Authority (DSHA) launched three new temporary programs to address barriers to achieving and maintaining homeownership for […]

Homeowners Seeking Financial Assistance Encouraged to Apply Now Dover, Del. January 9, 2024 – The Delaware Mortgage Relief Program, administered by the Delaware State Housing Authority (DSHA), is no longer accepting new applications for financial assistance to cover delinquent homeowner association fees, homeowner’s insurance, and lot rent fees. Applications for monthly payment assistance will end […]

WILMINGTON – The Delaware Department of Services for Children, Youth and Their Families is sharing the agency’s newly redesigned website. Launched Thursday, July 29, the website redesign of kids.delaware.gov was in coordination with the Department’s rebranding over the last year, which has included a new, updated logo. Throughout this process, DSCYF worked with the Department […]

The archbishop of Canterbury, Justin Welby, resigned over an abuse cover-up scandal on Tuesday, marking an unprecedented moment for the Church of England.

Medvedev, who lifted the title in 2020, did not face a single break point and broke De Minaur's serve three times.

WILMINGTON, Del. – Small businesses and nonprofit organizations in New Castle County affected by flooding from Hurricane Ida earlier this month are now eligible to apply for an Economic Injury Disaster Loan from the U.S. Small Business Administration (SBA). Eligible businesses and nonprofits may qualify for loans up to $2 million. The SBA Economic Injury […]

In light of a federal child pornography indictment filed in Pennsylvania against former Salesianum School principal William McCandless, the Delaware Department of Justice is reminding the public of a dedicated DOJ hotline and e-mail address for potential victims of abuse to contact . The Department is closely monitoring this matter and asks any potential victims […]

Delaware’s bans on assault weapons, large capacity mags remain in effect  A federal judge hearing a right-wing challenge against two common-sense gun safety measures in Delaware has ruled against the gun lobby on a critical question in Delaware State Sportsmen’s Association, Inc. et al. v. Delaware Department of Safety and Homeland Security et al.  In an […]

A federal judge has denied another right-wing challenge to a commonsense gun safety law that allows gun dealers in Delaware to be held accountable in court for the violence they create.   This is Attorney General Kathy Jennings’ third consecutive legal win over the gun lobby.  Earlier this year, the same federal court ruled against plaintiffs challenging Delaware’s assault weapons ban and large-capacity magazine ban.  Both remain in effect, as well.  “Sometimes the facts of a case tell you all you need to know,” […]

District Court sends stolen ammo investigation back to Superior Court, awards DOJ legal fees A U.S. District Court Judge has rejected an attempt by Cabela’s Inc. to hinder the Delaware Attorney General’s investigation into reports of 500,000 rounds of ammunition shoplifted from the retailer’s Christiana location.  Attorney General Kathy Jennings announced last summer that she […]

The Delaware Department of Labor will be receiving a grant from the U.S. Department of Labor to provide job training and job search assistance to displaced workers who have been challenged by residual effects from the economic downturn.

New online tool will support all Delawareans seeking new employment, fits into state effort to help ex-DuPont employees Wilmington, DE – The United States Department of Labor has announced that it has awarded the Delaware Department of Labor a $693,357 grant to support dislocated workers through the development of a new web-based application that will […]

In the event of a Federal government shutdown, Federal employees may be eligible for Unemployment Compensation for Federal Employees (UCFE). The UCFE program is administered by state unemployment insurance (UI) agencies acting as agents of the Federal government. The program is operated under the same terms and conditions that apply to regular state UI. In […]

Loan program would protect federal workers and their families during government shutdown DOVER, Del. – Governor John Carney and members of the Delaware General Assembly announced legislation on Tuesday to help federal workers who live in Delaware and are affected by the ongoing shutdown of the federal government. House Bill 3, sponsored by House Majority […]

In observance of Worker’s Memorial Day, the Delaware Department of Labor’s (DOL) Office of Safety and Health Consultation is proud to announce the launch of SafeDE (pronounced Safe-DEE-EEE). This groundbreaking initiative signifies our dedication to enhancing workplace safety throughout Delaware, honoring the memory of workers who have tragically lost their lives on the job. SafeDE […]

TOWNSEND, Del. (September 7, 2022) – Volunteers are needed to plant 2,000 hardwood seedlings along the Cypress Branch at Blackbird State Forest on Saturday, September 17 to provide scenic beauty, enhance wildlife habitat, fight invasive species, and improve water quality in the critical Chesapeake Bay Watershed. State Forester Michael Valenti said, “Planting trees is a […]

La agencia también lanza una campaña de marketing para animar a las personas con discapacidades y a las personas mayores a vacunarse WILMINGTON (14 de Diciembre de 2021) – El Departamento de Salud y Servicios Sociales (DHSS) lanzó hoy un número gratuito para que los habitantes de Delaware soliciten vacunas de COVID-19 para personas mayores […]

DOVER, DE (June 18, 2022) – Parents of children ages 6 months through 5 years old, will be able to get their children vaccinated against COVID-19 starting the week of June 20, 2022, according to the Division of Public Health (DPH). Vaccines for this youngest age group received final sign off for Emergency Use Authorization, from the Food […]

As businesses in the UK and Ireland rapidly adopt generative AI, strategic insights from the latest SAS study reveal the roadmap to successful integration and the hurdles to overcome. GenAI is rapidly transforming how businesses operate, innovate, and interact with customers and employees alike. However, as the technology proliferates, so [...]

Unlocking generative AI: Navigating challenges to reap unprecedented business benefits was published on SAS Voices by Iain Brown

Smyrna – Governor John Carney submitted a formal request yesterday to President Donald Trump, asking for a Presidential Disaster Declaration for the State of Delaware as a result of the severe weather events during the period of August 4, 2020 through August 7, 2020.  In his letter to President Trump, the Governor noted over four […]