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Multi-species cryoEM calibration and workflow verification standard

Cryogenic electron microscopy (cryoEM) is a rapidly growing structural biology modality that has been successful in revealing molecular details of biological systems. However, unlike established biophysical and analytical techniques with calibration standards, cryoEM has lacked comprehensive biological test samples. Here, a cryoEM calibration sample consisting of a mixture of compatible macromolecules is introduced that can not only be used for resolution optimization, but also provides multiple reference points for evaluating instrument performance, data quality and image-processing workflows in a single experiment. This combined test specimen provides researchers with a reference point for validating their cryoEM pipeline, benchmarking their methodologies and testing new algorithms.




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Understanding secondary order parameters in perovskites with tilted octahedra

A symmetry guide for the secondary structural degrees of freedom and related physical properties generated by tilts of BX6 octahedra in perovskites is proposed.




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Understanding secondary order parameters in perovskites with tilted octahedra

In the family of perovskite materials, the tilts of BX6 octahedra are the most common type of structural distortion. Conventionally, the formation of low-symmetry perovskite phases with tilted octahedra is analyzed by considering only primary order parameters. However, octahedral tilting also gives rise to secondary order parameters which contribute to additional atomic displacements, ordering and lattice distortions. Our study highlights the significant impact of secondary order parameters on the structural formation and emergent physical properties of perovskites. Through group-theoretical and crystallographic analyses, we have identified all secondary order parameters within Glazer-type tilt systems and clarified their physical manifestations. We explore the fundamental symmetry relationships among various structural degrees of freedom in perovskites, including tilt-induced ferroelasticity, correlations between displacements and ordering of atoms occupying different positions, and the potential for rigid unit rotations and unconventional octahedral tilts. Particular emphasis is placed on the emergence of secondary order parameters and their coupling with primary order parameters, as well as their symmetry-based hierarchy, illustrated through a modified Bärnighausen tree. We applied our theoretical insights to elucidate phase transitions in well known perovskites such as CaTiO3 and RMnO3 (where R = La and lanthanide ions), thereby demonstrating the significant influence of secondary order parameters on crystal structure formation. Our results serve as a symmetry-based guide for the design, identification and structural characterization of perovskites with tilted octahedra, and for understanding tilt-induced physical properties.




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Symmetries and symmetry-generated averages of elastic constants up to the sixth order of nonlinearity for all crystal classes, isotropy and transverse isotropy

Algebraic expressions for averaging linear and nonlinear stiffness tensors from general anisotropy to different effective symmetries (11 Laue classes elastically representing all 32 crystal classes, and two non-crystalline symmetries: isotropic and cylindrical) have been derived by automatic symbolic computations of the arithmetic mean over the set of rotational transforms determining a given symmetry. This approach generalizes the Voigt average to nonlinear constants and desired approximate symmetries other than isotropic, which can be useful for a description of textured polycrystals and rocks preserving some symmetry aspects. Low-symmetry averages have been used to derive averages of higher symmetry to speed up computations. Relationships between the elastic constants of each symmetry have been deduced from their corresponding averages by resolving the rank-deficient system of linear equations. Isotropy has also been considered in terms of generalized Lamé constants. The results are published in the form of appendices in the supporting information for this article and have been deposited in the Mendeley database.




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High-throughput and high-resolution powder X-ray diffractometer consisting of six sets of 2D CdTe detectors with variable sample-to-detector distance and innovative automation system

The demand for powder X-ray diffraction analysis continues to increase in a variety of scientific fields, as the excellent beam quality of high-brightness synchrotron light sources enables the acquisition of high-quality measurement data with high intensity and angular resolution. Synchrotron powder diffraction has enabled the rapid measurement of many samples and various in situ/operando experiments in nonambient sample environments. To meet the demands for even higher throughput measurements using high-energy X-rays at SPring-8, a high-throughput and high-resolution powder diffraction system has been developed. This system is combined with six sets of two-dimensional (2D) CdTe detectors for high-energy X-rays, and various automation systems, including a system for automatic switching among large sample environmental equipment, have been developed in the third experimental hutch of the insertion device beamline BL13XU at SPring-8. In this diffractometer system, high-brilliance and high-energy X-rays ranging from 16 to 72 keV are available. The powder diffraction data measured under ambient and various nonambient conditions can be analysed using Rietveld refinement and the pair distribution function. Using the 2D CdTe detectors with variable sample-to-detector distance, three types of scan modes have been established: standard, single-step and high-resolution. A major feature is the ability to measure a whole powder pattern with millisecond resolution. Equally important, this system can measure powder diffraction data with high Q exceeding 30 Å−1 within several tens of seconds. This capability is expected to contribute significantly to new research avenues using machine learning and artificial intelligence by utilizing the large amount of data obtained from high-throughput measurements.




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Diisobutyl­ammonium tri­phenyl(2-thiolato­acetato-κ2O,S)stannate(IV)

Crystals of the title salt, (C8H20N)[Sn(C6H5)3(C2H2O2S)], comprise diisobutyl­ammonium cations and mercapto­acetato­tri­phenyl­stannate(IV) anions. The bidentate binding mode of the mercapto­acetate ligand gives rise to a five-coordinated, ionic tri­phenyl­tin complex with a distorted cis-trigonal–bipyramidal geometry around the tin atom. In the crystal, charge-assisted ammonium-N—H⋯O(carboxyl­ate) hydrogen-bonding connects two cations and two anions into a four-ion aggregate. Two positions were resolved for one of the phenyl rings with the major component having a site occupancy factor of 0.60 (3).




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Bis(2-carb­oxy­quinolinium) hexa­chlorido­stan­nate(IV) dihydrate

In the hydrated title salt, (C10H8NO2)2[SnCl6]·2H2O, the tin(IV) atom is located about a center of inversion. In the crystal structure, the organic cation, the octa­hedral inorganic anion and the water mol­ecule of crystallization inter­act through O—H⋯O, N—H⋯O and O—H⋯Cl hydrogen bonds, supplemented by weak π–π stacking between neighboring cations, and C—Cl⋯π inter­actions.




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Using cryo-EM to understand the assembly pathway of respiratory complex I

Complex I (proton-pumping NADH:ubiquinone oxidoreductase) is the first component of the mitochondrial respiratory chain. In recent years, high-resolution cryo-EM studies of complex I from various species have greatly enhanced the understanding of the structure and function of this important membrane-protein complex. Less well studied is the structural basis of complex I biogenesis. The assembly of this complex of more than 40 subunits, encoded by nuclear or mitochondrial DNA, is an intricate process that requires at least 20 different assembly factors in humans. These are proteins that are transiently associated with building blocks of the complex and are involved in the assembly process, but are not part of mature complex I. Although the assembly pathways have been studied extensively, there is limited information on the structure and molecular function of the assembly factors. Here, the insights that have been gained into the assembly process using cryo-EM are reviewed.




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A database overview of metal-coordination distances in metalloproteins

Metalloproteins are ubiquitous in all living organisms and take part in a very wide range of biological processes. For this reason, their experimental characterization is crucial to obtain improved knowledge of their structure and biological functions. The three-dimensional structure represents highly relevant information since it provides insight into the interaction between the metal ion(s) and the protein fold. Such interactions determine the chemical reactivity of the bound metal. The available PDB structures can contain errors due to experimental factors such as poor resolution and radiation damage. A lack of use of distance restraints during the refinement and validation process also impacts the structure quality. Here, the aim was to obtain a thorough overview of the distribution of the distances between metal ions and their donor atoms through the statistical analysis of a data set based on more than 115 000 metal-binding sites in proteins. This analysis not only produced reference data that can be used by experimentalists to support the structure-determination process, for example as refinement restraints, but also resulted in an improved insight into how protein coordination occurs for different metals and the nature of their binding interactions. In particular, the features of carboxylate coordination were inspected, which is the only type of interaction that is commonly present for nearly all metals.




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Crystal structure of vancomycin bound to the resistance determinant d-alanine-d-serine

Vancomycin is a glycopeptide antibiotic that for decades has been a mainstay of treatment for persistent bacterial infections. However, the spread of antibiotic resistance threatens its continued utility. In particular, vancomycin-resistant enterococci (VRE) have become a pressing clinical challenge. Vancomycin acts by binding and sequestering the intermediate Lipid II in cell-wall biosynthesis, specifically recognizing a d-alanine-d-alanine dipeptide motif within the Lipid II molecule. VRE achieve resistance by remodeling this motif to either d-alanine-d-lactate or d-alanine-d-serine; the former substitution essentially abolishes recognition by vancomycin of Lipid II, whereas the latter reduces the affinity of the antibiotic by roughly one order of magnitude. The complex of vancomycin bound to d-alanine-d-serine has been crystallized, and its 1.20 Å X-ray crystal structure is presented here. This structure reveals that the d-alanine-d-serine ligand is bound in essentially the same position and same pose as the native d-alanine-d-alanine ligand. The serine-containing ligand appears to be slightly too large to be comfortably accommodated in this way, suggesting one possible contribution to the reduced binding affinity. In addition, two flexible hydroxyl groups – one from the serine side chain of the ligand, and the other from a glucose sugar on the antibiotic – are locked into single conformations in the complex, which is likely to contribute an unfavorable entropic component to the recognition of the serine-containing ligand.




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Cocrystals of a coumarin derivative: an efficient approach towards anti-leishmanial cocrystals against MIL-resistant Leishmania tropica

Leishmaniasis is a neglected parasitic tropical disease with numerous clinical manifestations. One of the causative agents of cutaneous leishmaniasis (CL) is Leishmania tropica (L. tropica) known for causing ulcerative lesions on the skin. The adverse effects of the recommended available drugs, such as amphotericin B and pentavalent antimonial, and the emergence of drug resistance in parasites, mean the search for new safe and effective anti-leishmanial agents is crucial. Miltefosine (MIL) was the first recommended oral medication, but its use is now limited because of the rapid emergence of resistance. Pharmaceutical cocrystallization is an effective method to improve the physicochemical and biological properties of active pharmaceutical ingredients (APIs). Herein, we describe the cocrystallization of coumarin-3-carb­oxy­lic acid (CU, 1a; 2-oxobenzo­pyrane-3-carb­oxy­lic acid, C10H6O4) with five coformers [2-amino-3-bromo­pyridine (1b), 2-amino-5-(tri­fluoro­methyl)-pyridine (1c), 2-amino-6-methyl­pyridine (1d), p-amino­benzoic acid (1e) and amitrole (1f)] in a 1:1 stoichiometric ratio via the neat grinding method. The cocrystals 2–6 obtained were characterized via single-crystal X-ray diffraction, powder X-ray diffraction, differential scanning calorimetry and thermogravimetric analysis, as well as Fourier transform infrared spectroscopy. Non-covalent interactions, such as van der Waals, hydrogen bonding, C—H⋯π and π⋯π interactions contribute significantly towards the packing of a crystal structure and alter the physicochemical and biological activity of CU. In this research, newly synthesized cocrystals were evaluated for their anti-leishmanial activity against the MIL-resistant L. tropica and cytotoxicity against the 3T3 (normal fibroblast) cell line. Among the non-cytotoxic cocrystals synthesized (2–6), CU:1b (2, IC50 = 61.83 ± 0.59 µM), CU:1c (3, 125.7 ± 1.15 µM) and CU:1d (4, 48.71 ± 0.75 µM) appeared to be potent anti-leishmanial agents and showed several-fold more anti-leishmanial potential than the tested standard drug (MIL, IC50 = 169.55 ± 0.078 µM). The results indicate that cocrystals 2–4 are promising anti-leishmanial agents which require further exploration.




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Crystal structure of (1,4,7,10,13,16-hexa­oxa­cycloocta­decane-κ6O)potassium-μ-oxalato-tri­phenylstannate(IV), the first reported 18-crown-6-stabilized potassium salt of tri­phenyl­oxalatostannate

The title complex, (1,4,7,10,13,16-hexa­oxa­cyclo­octa­decane-1κ6O)(μ-oxalato-1κ2O1,O2:2κ2O1',O2')triphenyl-2κ3C-potassium(I)tin(IV), [KSn(C6H5)3(C2O4)(C12H24O6)] or K[18-Crown-6][(C6H5)3SnO4C2], was synthesized. The complex consists of a potassium cation coordinated to the six oxygen atoms of a crown ether mol­ecule and the two oxygen atoms of the oxalatotri­phenyl­stannate anion. It crystallizes in the monoclinic crystal system within the space group P21. The tin atom is coordinated by one chelating oxalate ligand and three phenyl groups, forming a cis-trigonal–bipyramidal geometry around the tin atom. The cations and anions form ion pairs, linked through carbonyl coordination to the potassium atoms. The crystal structure features C—H⋯O hydrogen bonds between the oxygen atoms of the oxalate group and the hydrogen atoms of the phenyl groups, resulting in an infinite chain structure extending along a-axis direction. The primary inter-chain inter­actions are van der Waals forces.




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X-ray standing wave characterization of the strong metal–support interaction in Co/TiOx model catalysts

The strong metal–support interaction (SMSI) is a phenomenon observed in supported metal catalyst systems in which reducible metal oxide supports can form overlayers over the surface of active metal nanoparticles (NPs) under a hydrogen (H2) environment at elevated temperatures. SMSI has been shown to affect catalyst performance in many reactions by changing the type and number of active sites on the catalyst surface. Laboratory methods for the analysis of SMSI at the nanoparticle-ensemble level are lacking and mostly based on indirect evidence, such as gas chemisorption. Here, we demonstrate the possibility to detect and characterize SMSIs in Co/TiOx model catalysts using the laboratory X-ray standing wave (XSW) technique for a large ensemble of NPs at the bulk scale. We designed a thermally stable MoNx/SiNx periodic multilayer to retain XSW generation after reduction with H2 gas at 600°C. The model catalyst system was synthesized here by deposition of a thin TiOx layer on top of the periodic multilayer, followed by Co NP deposition via spare ablation. A partial encapsulation of Co NPs by TiOx was identified by analyzing the change in Ti atomic distribution. This novel methodological approach can be extended to observe surface restructuring of model catalysts in situ at high temperature (up to 1000°C) and pressure (≤3 mbar), and can also be relevant for fundamental studies in the thermal stability of membranes, as well as metallurgy.




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Laboratory-based 3D X-ray standing-wave analysis of nanometre-scale gratings

The increasing structural complexity and downscaling of modern nanodevices require continuous development of structural characterization techniques that support R&D and manufacturing processes. This work explores the capability of laboratory characterization of periodic planar nanostructures using 3D X-ray standing waves as a promising method for reconstructing atomic profiles of planar nanostructures. The non-destructive nature of this metrology technique makes it highly versatile and particularly suitable for studying various types of samples. Moreover, it eliminates the need for additional sample preparation before use and can achieve sub-nanometre reconstruction resolution using widely available laboratory setups, as demonstrated on a diffractometer equipped with a microfocus X-ray tube with a copper anode.




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Coordinate-based simulation of pair distance distribution functions for small and large molecular assemblies: implementation and applications

X-ray scattering has become a major tool in the structural characterization of nanoscale materials. Thanks to the widely available experimental and computational atomic models, coordinate-based X-ray scattering simulation has played a crucial role in data interpretation in the past two decades. However, simulation of real-space pair distance distribution functions (PDDFs) from small- and wide-angle X-ray scattering, SAXS/WAXS, has been relatively less exploited. This study presents a comparison of PDDF simulation methods, which are applied to molecular structures that range in size from β-cyclo­dextrin [1 kDa molecular weight (MW), 66 non-hydrogen atoms] to the satellite tobacco mosaic virus capsid (1.1 MDa MW, 81 960 non-hydrogen atoms). The results demonstrate the power of interpretation of experimental SAXS/WAXS from the real-space view, particularly by providing a more intuitive method for understanding of partial structure contributions. Furthermore, the computational efficiency of PDDF simulation algorithms makes them attractive as approaches for the analysis of large nanoscale materials and biological assemblies. The simulation methods demonstrated in this article have been implemented in stand-alone software, SolX 3.0, which is available to download from https://12idb.xray.aps.anl.gov/solx.html.




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Foreword to the special virtual issue on X-ray spectroscopy to understand functional materials: instrumentation, applications, data analysis




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Careem Pay introduces instant transfers for customers in Europe

Digital wallet and fintech platform Careem Pay has launched...




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Genome introduces SEPA Instant Transfers for Eurozone payments

Lithuania-based Genome has launched SEPA Instant Transfers,...





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Astronomers find a golden glow from a distant stellar collision

Full Text:

On August 17, 2017, scientists made history with the first direct observation of a merger between two neutron stars. It was the first cosmic event detected in both gravitational waves and the entire spectrum of light, from gamma rays to radio emissions. The impact also created a kilonova -- a turbocharged explosion that instantly forged several hundred planets’ worth of gold and platinum. The observations provided the first compelling evidence that kilonovae produce large quantities of heavy metals, a finding long predicted by theory. Astronomers suspect that all of the gold and platinum on Earth formed as a result of ancient kilonovae created during neutron star collisions. Based on data from the 2017 event, first spotted by the Laser Interferometer Gravitational-wave Observatory (LIGO), astronomers began to adjust their assumptions of how a kilonova should appear to Earth-bound observers. A team of scientists reexamined data from a gamma-ray burst spotted in August 2016 and found new evidence for a kilonova that went unnoticed during the initial observations.

Image credit: NASA/ESA/E. Troja




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New way for bridges to withstand earthquakes: Support column design

Full Text:

Bridges make travel faster and more convenient, but, in an earthquake, these structures are subject to forces that can cause extensive damage and make them unsafe. Now civil and environmental engineer Petros Sideris of Texas A&M University is leading a National Science Foundation (NSF)-funded research project to investigate the performance of hybrid sliding-rocking (HSR) columns. HSR columns provide the same support as conventional bridge infrastructure columns but are more earthquake-resistant. HSR columns are a series of individual concrete segments held together by steel cables that allow for controlled sliding and rocking. This allows the columns to shift without damage, while post-tensioning strands ensure that at the end of an earthquake the columns are pushed back to their original position. Conventional bridges are cast-in-place monolithic concrete elements that are strong but inflexible. Structural damage in these bridge columns, typically caused by a natural disaster, often forces a bridge to close until repairs are completed. But bridges with HSR columns can withstand large earthquakes with minimal damage and require minor repairs, likely without bridge closures. Such infrastructure helps with post-disaster response and recovery and can save thousands in taxpayer dollars. In an earthquake, HSR columns provide "multiple advantages to the public," Sideris said. "By preventing bridge damage, we can maintain access to affected areas immediately after an event for response teams to be easily deployed, and help affected communities recover faster. In mitigating losses related to post-event bridge repairs and bridge closures, more funds can be potentially directed to supporting the recovery of the affected communities." According to Joy Pauschke, NSF program director for natural hazards engineering, "NSF invests in fundamental engineering research so that, in the future, the nation's infrastructure can be more resilient to earthquakes, hurricanes, and other forces of nature."

Image credit: Texas A&M University




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Animal Shelter Assistant

Catawba County Emergency Services (Animal Shelter) is recruiting an experienced Animal Shelter Assistant with outstanding communication/customer service skills and office/clerical skills to join our growing Animal Services team.  As an Animal Shelter Assistant, you will direct the flow of animals, customers, and inquiries related to Animal Services; maintain and organize information and records; and assist with multiple aspects of shelter operations. Schedule is Tuesday-Saturday 9:00 am to 6:00 pm.  

Salary is negotiable within the listed range dependent upon qualifications (directly related experience).  




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Administrative Assistant I - Child Support

Catawba County Social Services is committed to making living better through serving children and families with multiple and complex needs.  
 
We are seeking an Administrative Assistant I for our Child Support unit who shares our common values:
 
Doing what’s right:
  • Provide the utmost Child Support Services by always providing excellent customer service to internal and external customers.
Doing what matters most:
  • Advocating for children to receive the financial stability that they deserve and providing the best customer service to all that we serve.
Doing it together:
  • Building relationships with all parties involved as well as stakeholders to obtain an order of support or to enforce an order support, so that children can receive the financial support they need and deserve.
Doing it well:
  • Striving for excellence in meeting all mandated standards in a fast – paced rewarding environment that ultimately seeks to provide financial stability for children, parents and caretakers.




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2021 Miss Nevada Will Be The First Openly Transgender Miss USA Contestant

Josie Fischels and Sarah McCammon | NPR

Wearing a rainbow sequin gown she designed herself in honor of Pride Month, Kataluna Enriquez made history last weekend when she was crowned Miss Nevada USA — the win will make her the first openly transgender contestant to compete in the upcoming Miss USA pageant this fall.

"My win is our win," she posted afterward on her Instagram in a message to the LGBTQ community. "We just made history. Happy pride."

Enriquez, who was also Miss Nevada USA's first trans contestant, beat out 21 other women for the top spot. She will represent the Silver State at the 2021 Miss USA pageant that will be held on Nov. 29 in Tulsa, Okla., where she will have a chance to be crowned Miss USA and advance to the Miss Universe pageant.

If crowned Miss USA, Enriquez will become the second trans contestant to compete for Miss Universe, after Angela Ponce, who represented Spain in the 2018 Miss Universe pageant. The pageant began allowing transgender contestants in 2012.

Enriquez began competing in pageants in 2015. Unable to afford custom designer gowns that fit her body at the time, she began designing her own to wear for competitions and eventually started her own line, Kataluna Kouture (@katalunakouture). In March, Enriquez became the first trans woman to win Miss Nevada's preliminary pageant, Miss Silver State USA.

The journey has not been easy, and Enriquez has faced discrimination. While competing in a pageant outside of Nevada, she had not been given a roommate when pageant organizers learned she was trans. A doctor had also been sent to certify that she was a woman before she could continue.

But Enriquez told NPR's Weekend All Things Considered that her determination to make history was what motivated her to keep competing.

"I had a purpose and I had a dream," she said. "I wanted to compete on the Miss USA stage. When I was young, I always wanted to see someone on the Miss USA stage — someone like me. And it just happened to be that I was the person that I needed to make history."

As she prepares for the Miss USA pageant, Enriquez said she plans to advocate for equality and mental health.

"My win is not just a win for the trans community," she said. "It's a win for all women to be represented."

Kalyani Saxena and Tinbete Ermyas produced and edited the audio version of this story. Josie Fischels produced for the web. Josie Fischels is an intern on NPR's News Desk.

Copyright 2021 NPR. To see more, visit https://www.npr.org.

This content is from Southern California Public Radio. View the original story at SCPR.org.




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I-95 Partially Closed For Hours After Police Standoff With Armed Men

Traffic on Interstate 95 was diverted for hours after a group of armed men fled from police near Wakefield, Mass. on Saturday. Massachusetts state police say 11 suspects have been taken into custody.; Credit: Michael Dwyer/AP

Matthew S. Schwartz | NPR

Eleven people were taken into custody Saturday after an hours-long standoff with police early Saturday outside Boston.

While police engaged in negotiation, members of the group engaged the public on social media, saying their group was called "Rise of the Moors."

The heavily armed men were said to be driving from Rhode Island to Maine for "training."

The incident started around 1:30 a.m., when state police noticed two cars stopped at the side of I-95 near Wakefield, Mass., apparently out of fuel. As troopers stopped to assist, they noticed that some individuals near the cars had "military-style" gear, and were carrying long guns and pistols, Massachusetts State Police Col. Christopher Mason told reporters.

"You can imagine 11 armed individuals standing with long guns slung on an interstate highway at 2 in the morning certainly raises concerns and is not consistent with the firearms laws that we have in Massachusetts," Mason said.

Police requested backup, and thus began a standoff that lasted several hours. The men refused to lower their weapons, saying that they "don't recognize our laws," police said. Some of the armed men fled into a nearby wooded area, police said, and a portion of I-95 was closed for several hours.

The standoff was broadcast on social media

Around 4 a.m., a man whom the Boston Globe identified as Jamhal Talib Abdullah Bey said he was broadcasting live from I-95 and said that he told police they had nothing to fear.

"I reassured them that we are not sovereign citizens," a man who appeared to be Bey said in a live-streamed video. "I reassured them that we are not Black identity extremists. I reassured them that we are not anti-police. I reassured them that we are not anti-government. I reassured them that these men here will not be pointing guns at them. I reassured them that we are trying to come to a peaceful resolution."

"We're going to our private land to train, which is our Second Amendment right," he said, showing a vehicle he said contained camping equipment.

As police tactical teams brought in armored vehicles to surround the area, and negotiators interacted with the men, they ultimately surrendered. Middlesex County District Attorney Marian Ryan told reporters the suspects were expected to appear in court on Tuesday morning.

State police "don't have any knowledge about this particular specific group" but as state police "it is not unusual for us to encounter people that have sovereign citizen ideologies — I'm not saying that this group does — but we have had those encounters before in the past," Mason said at a Saturday morning press briefing.

"We train to those encounters," Mason continued. "We very much understand the philosophy that underlies that mindset. And we train our officers, actually, at the academy, on these interactions and how to de-escalate those situations, and how to engage with people that have that philosophy and mindset and resolve those situations in a peaceful manner."

The group calls itself "Rise of the Moors"

The same man appearing to be Bey said in a later video: "They keep portraying us as being anti-government, but we're not anti-government at all."

The group's website lists Bey as a leader of the "Rhode Island State Republic and Providence Plantations." According to the site, Bey served in the military for four years, some or all of that time in the Marines, after which he began studying "Moorish Science."

That website, "Rise of the Moors," explains that Moors are not "sovereign citizens" because "sovereignty does not stand alone," but can rather be considered synonymous with "nationality."

"The record show that the Moors are the organic or original sovereigns of this land — America," the FAQ says. "When we declare our nationality as Moorish Americans we are taking back the position as the aboriginal people of the land, to which the sovereign power is vested in."

Bey's group may be associated with the Moorish sovereign citizen movement, which the Southern Poverty Law Center characterizes as an offshoot of the antigovernment sovereign citizens movement. Moorish sovereigns "have come into conflict with federal and state authorities over their refusal to obey laws and government regulations," SPLC writes.

"The Moorish Sovereign movement is a rapidly growing group of people who believe that they belong to a sovereign nation that has a treaty with the US but otherwise operates outside of the federal and state laws," JJ MacNab, a fellow at George Washington University's Program on Extremism, explained on Twitter.

"They rely on an alternative history that borrows from Moorish Science Temple, Black Hebrew Israelism, Nation of Islam, UFO theories, phony Native American tribes, and the pseudo-legal arguments crafted by white supremacist 'patriot' groups in the 1970s," MacNab said.

Copyright 2021 NPR. To see more, visit https://www.npr.org.

This content is from Southern California Public Radio. View the original story at SCPR.org.




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Magenta Mobility raises USD 22 mn from bp, Morgan Stanley India Infra

The fresh capital infusion takes the total fund raised by the company to Rs 275 crore, with as much as Rs 95 crore mopped up in various rounds earlier including from Indian-American philanthropist and serial entrepreneur Kiran Patel.




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Lee Worsley, Assistant County Manager in Catawba County since 2005, named Deputy County Manager in Durham County.

During his tenure with Catawba County, Worsley has had a positive impact in many areas, including emergency services, the use of emerging technologies, and mentoring people who are just starting out in local government management.




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Rita Beaver, Assistant Register of Deeds, named Catawba County 2011 Emloyee Of The Year

A 35 year veteran of the Register of Deeds Office, Beaver was praised by all her co-workers in that office for excellent customer service, for being a patient instructor on the laws involved in her work, and for preserving the County�s history.




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Catawba County names new Assistant County Manager

Mary Furtado is currently serving as Executive Director of Strategic Operations for Sarasota County, Florida. She will begin her new duties in Catawba County on December 12, 2011.




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Program available for persons working at least 20 hours a week but need assistance with car repairs or insurance

The program of the North Carolina Department of Transportation provides limited funds for low-income persons who need to maintain or insure their car so they can continue working. It is administered by Catawba County Social Services




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Catawba County Finance Officer named Outstanding NC Finance Officer Of Year

Rodney Miller has been named Outstanding N.C. Finance Officer of the Year for 2011-12 by the North Carolina Government Finance Officers Association. The award traditionally and annually recognizes and honors the local government finance officer who has demonstrated state leadership in local government finance issues by serving as the organization's president.




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Catawba Co. Assistant Planning Director, Mary George, among leaders of nationally recognized river conservation effort.

The Institute for Conservation Leadership has chosen to honor the Catawba-Wateree Relicensing Coalition for their exemplary collaboration to accomplish outstanding environmental protection. The Coalition is being recognized for collaborative work that is creative, visionary, and highly effective and that their respective coalition members could not have achieved by acting alone




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Catawba County Assistant Planning Director, Mary George, named 2012 Outstanding Contributor to Agriculture.

Catawba County Assistant Planning Director, Mary George, has been named 2012 Outstanding Contributor to Agriculture by the Hickory Kiwanis Club




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Assistant County Manager Dewey Harris earns international Credentialed Manager distinction.

Catawba County Assistant County Manager Dewey Harris has earned the International City/County Management Association's (ICMA) Credentialed Manager designation. Established in 2002, the ICMA Credentialed Manager program recognizes professional government managers whom the ICMA certifies as having a "commitment to continuous learning and professional development".




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Catawba County requests State assistance for those with damage from July 27 flooding.

Catawba County requests State assistance for those with damage from July 27 flooding.




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Budget Office again wins national award, for producing budget document judged easy to read and understand.

Budget Office wins national award, for producing budget document judged easy to read and understand, for 25th straight year




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Pandemic Pomp and Circumstance: Graduation Looks Different This Year (Again)

; Credit: /Michelle Kondrich for NPR

Elissa Nadworny and Eda Uzunlar | NPR

In Jasmine Williams' family, graduating from the University of Michigan is a rite of passage. Her parents met on the campus, and her older sister graduated from the school a few years ago. She remembers sitting bundled up in the family section for that graduation. "It was overwhelming to feel so many people that proud," she says, "I remember sitting there watching her, and that was probably the first time I was like, 'OK, yeah, I like this. I can't wait to do this.'

This year, Williams' own graduation will look a bit different. The main undergraduate ceremony will be all virtual, though the university has invited students to watch that ceremony from the football stadium on campus known as the Big House. There will be no family members in attendance, and students will be required to have a negative COVID-19 test result to enter.

"I think it's hard not to downplay it when it's reduced to a Zoom," says Williams. But come Saturday, she's planning on donning her cap and gown and heading to the stadium with friends. "Knowing that we are going to the Big House to watch together as a class makes everything way more enjoyable for the weekend; to be able to at least get some remnants of what I witnessed years ago with my sister." Her family plans to host a streaming party from their home in Detroit.

As an academic year like no other comes to an end, colleges and universities are celebrating their graduates in a variety of ways. Some schools, like the University of Idaho and Virginia Tech are hosting multiple smaller, in-person ceremonies to comply with social distancing mandates. Others, like Iowa State, are hosting large ceremonies in football stadiums and outdoor arenas. There's also a handful that are doing virtual-only again, like the University of Washington and Portland State University. At some schools, including the University of Michigan and Emmanuel College in Boston, in-person events are restricted to just graduates; family and friends have to watch from a livestream.

For lots of students, the effort to be in-person is greatly appreciated. "You work hard those four years, you dream of that day, getting to graduate in-person and walk across the stage," says Jamontrae Christmon, a graduating senior at Tennessee State University in Nashville. For most of the year he assumed graduation would be virtual. He even sent out his graduation announcements to friends and family — and left the date off. Weeks later, he learned TSU would actually hold a May 1st in-person ceremony in the football stadium.

"I haven't been sleeping much this week at all. I'm just happy. Excited," says Christmon.

But planning for an event in an ongoing public health emergency has proven to be stressful. Steve Bennett, the chief of staff for academic affairs at Syracuse University, has worked to create commencement ceremonies that are as close to a normal year as possible.

"This may be the single most challenging special event that our team has put together, maybe ever," explains Bennett. "And it's because we keep having to plan towards a moving target."

Syracuse's plan for graduation is to have multiple smaller commencement ceremonies in their stadium; everyone in attendance has to be fully vaccinated or show proof of a recent negative COVID-19 test. According to state guidance, the stadium can only reach 10 % capacity, so graduates are limited to two guests per person. Despite the restrictions, the team that planned the ceremonies is determined to make it one that the class of 2021 deserves.

"The students have been through a lot this year. Graduating seniors lost a number of student experiences due to pandemic conditions that are important to them," says Bennett. That's why having the in-person component was essential. "It was really important to the university, given [the seniors'] commitment to us, that we have a commitment to them."

At California Lutheran University, in Thousand Oaks, Calif., graduation will be celebrated as a drive-in style event at the Ventura County Fairgrounds. Each graduate can bring one carful of people to the fairgrounds parking lot, which can accommodate up to 700 vehicles. Inspired by the city's drive-in concert events, there'll be a stage with speakers and a jumbo screen.

"That's ultimately what led us to our decision to have it at the fairgrounds. Since it's a drive-in and they're staying in their cars, they were allowed to bring family... that was just really important to us," says Karissa Oien, who works in academic affairs at California Lutheran University and is the lead organizer for the drive-in commencement. She's been planning the university's ceremonies for 13 years, and knows how important graduation can be — not just for students, but for those who helped them along the way as well.

"We wanted to have that moment again. Where the families can see their students cross the stage and be there with them."

Jamontrae Christmon, the graduating senior from Tennessee State University, will have his parents, an uncle and one of his sisters there with him at Hale Stadium. "It's just something about your parents being there," says Christmon, "you want to look into the audience and maybe see your parents and you hear them scream your name when they call your name to walk across the stage."

As the day gets closer, Christmon says he's been thinking about the moments of self-doubt he had along the way. "I could have easily said 'I'm not cut out for college' and just gave up, but I didn't." He says his family was a big part of that motivation.

"Not many in my family even attended college, let alone graduated. So this is a big deal," says Christmon. "To me it means I broke the cycle. And that's what they always wanted."

He expects his mother will cry, and likely, he will too.

Copyright 2021 NPR. To see more, visit https://www.npr.org.

This content is from Southern California Public Radio. View the original story at SCPR.org.




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