Yinlong Song, Yikan Zhang, Ying Pan, Jianfeng He, Yan Wang, Wei Chen, Jing Guo, Haiteng Deng, Yi Xue, Xianyang Fang, and Xin Liang

Microtubules dynamics is regulated by the plus end-tracking proteins (+TIPs) in cells. End binding protein 1 (EB1) acts as a master regulator in +TIPs networks by targeting microtubule growing ends and recruiting other factors. However, the molecular mechanism of how EB1 binds to microtubule ends with a high affinity remains to be an open question. Using single-molecule imaging, we show that the end-binding kinetics of EB1 changes along with the polymerizing and hydrolysis rate of tubulin dimers, confirming the binding of EB1 to GTP/GDP-Pi tubulin at microtubule growing ends. The affinity of wild-type EB1 to these sites is higher than monomeric EB1 mutants, suggesting that two CH domains in the dimer contribute to the end-binding. Introducing phosphomimicking mutations into the linker domain of EB1 weakens the end-binding affinity and confers a more curved conformation to EB1 dimer without compromising dimerization, suggesting that the overall architecture of EB1 is important for the end-binding affinity. Taken together, our results provide insights into understanding how the high-affinity end-binding of EB1 can be achieved and how this activity may be regulated in cells.

Benjamin Ziman, Peter Karabinis, Paul Barghouth, and Nestor J. Oviedo

Nutrient availability upon feeding leads to an increase in body size in the planarian Schmidtea mediterranea. However, it remains unclear how food consumption integrates with cell division at the organismal level. Here we show that Sirtuins is evolutionarily conserved in planarians and specifically demonstrate that Sirtuin-1 (Smed-Sirt-1) regulates organismal growth by impairing both feeding behavior and intestinal morphology. Disruption of Smed-Sirt-1 with either RNAi or pharmacological treatment leads to reduced animal growth. Conversely, enhancement of Smed-Sirt-1 with resveratrol accelerates growth. Differences in growth rates were associated with changes in the amount of time to locate food and overall consumption. Furthermore, Smed-Sirt-1(RNAi) animals displayed reduced cell death and increased stem cell proliferation accompanied by impaired expression of intestinal lineage progenitors and reduced branching of the gut. Altogether, our findings indicate Sirtuin-1 is a crucial metabolic hub capable of controlling animal behavior, tissue renewal and morphogenesis of the adult intestine.

The structural and nonlinear optical properties of a new anilinium hybrid crystal of chemical formula (C6H7NCl+·NO3−)3 have been investigated. The crystal structure was determined from single-crystal X-ray diffraction measurements performed at a temperature of 100 K which show that the compound crystallizes in a noncentrosymmetric space group (Pna21). The structural analysis was coupled with Hirshfeld surface analysis to evaluate the contribution of the different intermolecular interactions to the formation of supramolecular assemblies in the solid state that exhibit nonlinear optical features. This analysis reveals that the studied compound is characterized by a three-dimensional network of hydrogen bonds and the main contributions are provided by the O...H, C...H, H...H and Cl...H interactions, which alone represent ∼85% of the total contributions to the Hirshfeld surfaces. It is noteworthy that the halogen...H contributions are quite comparable with those of the H...H contacts. The nonlinear optical properties were investigated by nonlinear diffuse femtosecond-pulse reflectometry and the obtained results were compared with those of the reference material LiNbO3. The hybrid crystals exhibit notable second (SHG) and third (THG) harmonic generation which confirms its polarity is generated by the different intermolecular interactions. These measurements also highlight that the THG signal of the new anilinium compound normalized to its SHG counterpart is more pronounced than for LiNbO3.

Norbert Volkmar
Apr 24, 2020; 133:jcs243519-jcs243519
REVIEW

The emerging science of gene drives has the potential to address environmental and public health challenges, but gene-drive modified organisms are not ready to be released into the environment and require more research in laboratories and highly controlled field trials, says a new report from the National Academies of Sciences, Engineering, and Medicine.

The number of patients in the U.S. awaiting organ transplantation outpaces the amount of transplants performed in the U.S., and many donated organs are not transplanted each year due to several factors, such as poor organ function, says a new report from the National Academies of Sciences, Engineering, and Medicine.

The National Academy of Medicine (NAM) today announced that more than 130 organizations across the U.S. -- including associations, hospital and medical systems, universities, and professional societies -- have joined NAM in declaring their commitment to reducing burnout and promoting well-being among clinicians.

An international, multidisciplinary organizing committee has been appointed to plan the Second International Summit on Human Genome Editing, which will take place Nov. 27-29 in Hong Kong.

On the eve of the Second International Summit on Human Genome Editing, we were informed of the birth of twins in China whose embryonic genomes had been edited.

In December 2015, the U.S. National Academy of Sciences and U.S. National Academy of Medicine, the Royal Society of the United Kingdom, and the Chinese Academy of Sciences hosted an international summit in Washington, D.C., to discuss scientific, ethical, and governance issues associated with human genome editing.

The National Academy of Medicine (NAM) announced today that more than 100 organizations across the U.S. — including community organizations, hospital and medical systems, academia, nonprofits, and health professional societies — have joined NAM in declaring their commitment to reversing national trends in opioid misuse and overdose.

A new report from the National Academies of Sciences, Engineering, and Medicine offers guidance to the Consumer Product Safety Commission (CPSC) on how to conduct a hazard assessment of nonpolymeric, additive organohalogen flame retardants (OFRs), which are used in some consumer products.

A new report from the National Academies of Sciences, Engineering and Medicine finds that the U.S. Environmental Protection Agencys (EPA) approach to its ongoing Integrated Risk Information System (IRIS) assessment plan is appropriate for synthesizing the scientific evidence and quantifying estimates of inorganic arsenic toxicity.

It is critical for the U.S. to continue its funding for the World Health Organization in the midst of the COVID-19 pandemic given the WHO’s lead role in coordinating an international response, especially in developing countries.

Populations of otters, grey seals and sea eagles are slowly recovering in Sweden, which is likely to be thanks in part to a ban on organochlorine chemicals, such as PCBs and DDT, in the 1970s, according to a new study. However, the research shows that negative effects of these chemicals on the reproductive health of female animals persisted for more than 15 years after the ban was introduced.

Image modeling for biomedical organs

Researchers have discovered high levels of plastic particles and fibres, as well as black carbon (BC), which is formed by the incomplete burning of fossil fuels, in the waters of the Jade Bay, an inshore basin off the coast of Germany in the Southern North Sea. The concentration of suspended particles are of concern because they have the potential to be ingested by fish and other marine life, and enter the food chain.

Biodiversity on organic farms is, on average, 34% higher than on conventional farms, according to a recent study. The researchers used data from a large number of studies to show that this figure has remained stable over the last 30 years.

A recent study compares the effects of organic, ‘natural’ fertilisers, such as compost, with mineral, synthetic fertilisers, such as urea, on N2O emissions from Mediterranean soil. It suggests that there is little difference between the fertilisers, but that pig slurry offers the best overall balance in terms of emissions and crop yield.

Despite the current economic situation, organic farming is a growth sector in the EU. A recent EU-funded study suggests support payments from the amended 2003 Common Agricultural Policy (CAP) for organic farming will continue to play an important role in Western European countries and will become increasingly significant in new Member States.

A recent study investigates the factors that affect a farmer’s decision to convert to organic farming in Latvia and Estonia. A combination of social factors, such as peer pressure, and economic influences, such as subsidies, were found to be more important than either factor individually.

Organic pesticides may not always be the most environmentally-friendly choice of pest control, according to recent research. Two new synthetic pesticides for controlling aphids were found to be less harmful to other species and more efficient than the two new organic pesticides tested in the study.

Little difference was found between organically grown tomatoes and tomatoes grown conventionally in greenhouses over a three year period, in terms of taste and nutritional value, according to a recent study in the Netherlands. Taste and nutrition were more dependent on the breed of tomato.

Converting grassland to arable land can reduce the diversity of nematodes, predatory mites, earthworms and enchytraeid worms in the soil, according to a study by Dutch researchers. However, they found that restoring arable land to grassland did not fully restore the diversity of these four groups during the course of the four-year study.

Managing landscapes effectively requires the involvement of a wide variety of stakeholders. The views and interests of these different groups can be effectively integrated by agri-environment 'collaboratives' — a type of bridging organisation which can be found in varying forms in Europe. Using data from Germany and the Netherlands, a study concludes that these groups make important contributions to landscape management, ranging from implementing policy to generating income.

Ocean acidification could damage the organs of Atlantic herring, as well as slow their growth and development, recent experiments show. It adds to the list of pressures currently threatening this commercially important species, including over-fishing and marine pollution.

BT Compute brings you a choice of data centre and expert services that enable you to deliver applications and services where and when you need them -- Global services delivered locally. Our hybrid cloud services blend intelligent network and compute resources giving you the IT infrastructure you need to adapt, quickly and cost effectively.

Last year my organization fell victim to a trans- national export invoice fraud. Since 99 percent of our sales come from exports, the cyber fraud dealt a big blow to our organization. We have a customer in South East Asia and my organization had business dealings with this customer for over five years.

Including civil society organisations (CSOs) when negotiating climate policy can mobilise public support for international agreements, a new study suggests. Using online surveys researchers found that the popular legitimacy of global climate governance decreases when CSOs are excluded.

‘Network bridging organisations’, such as farmer unions, Regional Nature Parks and Local Action Groups, promote cooperation between farmers, non-state collective actors and state actors under the Common Agricultural Policy. This study finds that farmers who have regular contact with these organisations show a higher commitment to long-term practice change. This could represent an opportunity to improve the effectiveness of payments for environmental services in Europe.

Public participation is an essential part of integrated water management. In a recent study, researchers following the development of a UK catchment management plan found greater cooperation between land managers and environmental regulatory bodies as a result of a participatory process.

Soil erosion in Italy could be reduced by 43% if Good Agricultural and Environmental Conditions (GAEC) were fully adopted, a recent study has found. Reducing soil erosion would also increase soil organic carbon stocks, particularly on cultivated sloping land.

Organic farming has often been found to have benefits for biodiversity, but the benefits can depend on the individual species and landscape in question. Research on vine farming in Italy suggests that the impact of organic methods on the number or diversity of pollinating insects may depend on local context. For example, the lack of connectivity between organic vine farms may limit benefits in some circumstances.

A recent study has examined the effects of different farming practices on bird numbers and species found on farmland during the winter in six European countries. Overall, the greatest number of birds and species were on organic farms, especially when the farms were in landscapes where 80-99 per cent of the land was used for agriculture.

Converting grassland to arable land can reduce the diversity of nematodes, predatory mites, earthworms and enchytraeid worms in the soil, according to a study by Dutch researchers. However, they found that restoring arable land to grassland did not fully restore the diversity of these four groups during the course of the four-year study.

The IUCN’s Red List Index (RLI) of threatened species can be used to measure the effectiveness of conservation organisations. This is according to a new study which used the index to assess an organisation’s conservation impact on 17 species. Eight of these species saw improvements in their threat status, whereas 16 would have seen no improvement at all, or even deterioration, if there was no conservation action.

The biodiversity and abundance of arbuscular mycorrhizal fungi — important soil organisms that can help plants to capture nutrients — is greater in organically managed soils with reduced tilling compared to conventional methods, a new Swiss study suggests. This illustrates the impacts that land management practices such as ploughing can have on soil biodiversity and the ecosystem services it provides.

There are several types of wildlife-friendly farming scheme, some of which are more prescriptive than others. A recent study compared the effects of different wildlife-friendly farming approaches, including organic farming, on pollination. The findings suggest that organic farming practices enhance pollination services but may compromise crop yield. ‘Conservation Grade’ farming schemes — biodiversity-focused practices funded by sales of labelled food products — can support both pollination and yield.

Almost a quarter of the world’s non-polar land surface experiences light pollution, and there is concern that this adversely affects illuminated ecosystems. Currently there is a global move from yellow sodium lighting to white LED lighting, which emits different wavelengths of light. A recent study found that LED artificial light at night (ALAN) reduced the biomass of periphyton by 62% in a freshwater drainage ditch in Westhavelland Nature Park, Brandenburg, Germany.

A new approach to assessing the risk posed by 500 organic chemicals potentially found in the surface waters of river basins across Europe has been developed. It allows pollutants of concern, including emerging substances, to be identified and prioritised by Member States for monitoring and action as required by the Water Framework Directive.

Populations of otters, grey seals and sea eagles are slowly recovering in Sweden, which is likely to be thanks in part to a ban on organochlorine chemicals, such as PCBs and DDT, in the 1970s, according to a new study. However, the research shows that negative effects of these chemicals on the reproductive health of female animals persisted for more than 15 years after the ban was introduced.

Recent research has found that silver nanoparticles in sewage sludge, which is used on agricultural land as a fertiliser, can be toxic to soil microorganisms. The researchers calculated that a maximum of 30mg of silver nanoparticles per kilogram of sludge can be applied to land before harm occurs, based on typical application rates in Germany of five tons per hectare of farmland every three years.

Persistent organic pollutants (POPs) are priority pollutants that pose a risk to human health, and can be passed on to children via breast milk. This study investigated how concentrations of POPs in breast milk vary worldwide by reviewing studies published between 1995 and 2011. They found that levels of polychlorinated biphenyls (PCBs) and dioxins in breast milk are higher in Europe and North America, whereas pesticides are more prevalent in Africa and Asia. The authors call for harmonisation of methodologies to enable high quality comparisons between studies.

Pollutants have been shown to alter the structure of bacterial communities in the coastal waters around the Alang-Sosiya shipbreaking yard in north-west India. The research analysed seawater from two sites near Alang-Sosiya and from pristine sea water taken 10 km from the coast. The results provide a clearer idea of changes to the microbial ecology near a large ship recycling yard.

Nanotechnology is a key enabling technology predicted to have many societal benefits, but there are also concerns about its risks to the environment. This study reviewed the effects of nanoparticles on soil microorganisms, showing that toxicity depends on the type of particle. The researchers make recommendations for improving environmental risk assessment, including performing experiments in soil and over longer time periods.

The majority of persistent organic pollutants (POPs) identified until now are banned or restricted around the world owing to concerns about their harm to ecosystems and human health. However, this is not the end of the story; even long-banned POPs still linger in the environment; others are still in use and are being directly emitted; and new POPs may be identified for which we have limited information. This Future Brief from Science for Environment Policy presents recent research into POPs’ potential impacts, the levels and future outlook for POPs in the environment and humans, and how we can reduce our use of POPs.

Soil is an overlooked source of persistent organic pollutants (POPs) for grazing cows, finds a new study of contaminated farms in Switzerland. The researchers tested a new modelling tool to track two specific environmental POPs — known as polychlorinated biphenyls (PCBs) and dioxins (PCDD/Fs) — as they moved from the farm environment into a cow’s body over time. The tool could be used to assess measures designed to decontaminate animals or to prevent contamination, such as grazing regimes that aim to reduce the risk of cows eating soil accidentally.

Silver nanoparticles present in the effluent from waste-water treatment plants could have toxic effects on aquatic organisms, new research suggests. The lab-based study tested the effects of nanoparticle-containing effluent on several crustacean and algae species. The researchers observed that epibenthic crustaceans (those living in or on sediments at the bottom of water bodies) were the most sensitive; notably, a 20–45% higher death rate was observed compared with those exposed to nanoparticle-free effluent.