Microchip Unveils Family Details and Opens Early Access Program for RISC-V Enabled Low-Power PolarFire SoC FPGA Family

670,000 households in Indonesia are benefitting from improved access to water and sanitation.

Telangana government has plans to infuse a sum of Rs 50,000 crore to uplift the infra status in Hyderabad in the next five years. KT Rama Rao, Minister for Urban Development and Municipal Affairs (MA&UD) has shared that the fund would be utilised for the development of the Hyderabad Urban Agglomeration area. A corpus of Rs 50,000 crore is being readied by the Telangana government, which would go into the development of the Hyderabad Urban Agglomeration (HUA) region. KT Rama Rao, Minister for Urban Development and Municipal Affairs (MA&UD) has shared the information. HUA region comprises the core of the city as well as its outskirts falling within the Outer […]

CES 2014, LAS VEGAS – HARMAN, the premium global audio and infotainment group (NYSE:HAR) debuted several new products for the mobile aftermarket today at CES, continuing its tradition of leadership for best-in-class car audio.

GENEVA MOTOR SHOW 2015 – HARMAN, the premium global audio, visual, infotainment and enterprise automation group (NYSE:HAR), will demo its expanded scalable embedded infotainment platform at the Geneva International Motor Show. The offering addresses the full spectrum of vehicle segments, including entry- to mid-level cars, with a feature-rich, automotive-grade platform that leverages the latest smartphone integration technologies such as Apple CarPlay, Android Auto™ and MirrorLink for best-in-class connectivity for the world’s automakers.

ABSTRACT

The initiation of Escherichia coli chromosomal DNA replication starts with the oligomerization of the DnaA protein at repeat sequences within the origin (ori) region. The amount of ori DNA per cell directly correlates with the growth rate. During fast growth, the cell generation time is shorter than the time required for complete DNA replication; therefore, overlapping rounds of chromosome replication are required. Under these circumstances, the ori region DNA abundance exceeds the DNA abundance in the termination (ter) region. Here, high ori/ter ratios are found to persist in (p)ppGpp-deficient [(p)ppGpp⁰] cells over a wide range of balanced exponential growth rates determined by medium composition. Evidently, (p)ppGpp is necessary to maintain the usual correlation of slow DNA replication initiation with a low growth rate. Conversely, ori/ter ratios are lowered when cell growth is slowed by incrementally increasing even low constitutive basal levels of (p)ppGpp without stress, as if (p)ppGpp alone is sufficient for this response. There are several previous reports of (p)ppGpp inhibition of chromosomal DNA synthesis initiation that occurs with very high levels of (p)ppGpp that stop growth, as during the stringent starvation response or during serine hydroxamate treatment. This work suggests that low physiological levels of (p)ppGpp have significant functions in growing cells without stress through a mechanism involving negative supercoiling, which is likely mediated by (p)ppGpp regulation of DNA gyrase.

IMPORTANCE Bacterial cells regulate their own chromosomal DNA synthesis and cell division depending on the growth conditions, producing more DNA when growing in nutritionally rich media than in poor media (i.e., human gut versus water reservoir). The accumulation of the nucleotide analog (p)ppGpp is usually viewed as serving to warn cells of impending peril due to otherwise lethal sources of stress, which stops growth and inhibits DNA, RNA, and protein synthesis. This work importantly finds that small physiological changes in (p)ppGpp basal levels associated with slow balanced exponential growth incrementally inhibit the intricate process of initiation of chromosomal DNA synthesis. Without (p)ppGpp, initiations mimic the high rates present during fast growth. Here, we report that the effect of (p)ppGpp may be due to the regulation of the expression of gyrase, an important enzyme for the replication of DNA that is a current target of several antibiotics.

Legionella pneumophila, the etiological agent of Legionnaires’ disease, employs an arsenal of hundreds of Dot/Icm-translocated effector proteins to facilitate replication within eukaryotic phagocytes. Several effectors, called metaeffectors, function to regulate the activity of other Dot/Icm-translocated effectors during infection. The metaeffector Lpg2505 is essential for L. pneumophila intracellular replication only when its cognate effector, SidI, is present. SidI is a cytotoxic effector that interacts with the host translation factor eEF1A and potently inhibits eukaryotic protein translation by an unknown mechanism. Here, we evaluated the impact of Lpg2505 on SidI-mediated phenotypes and investigated the mechanism of SidI function. We determined that Lpg2505 binds with nanomolar affinity to SidI and suppresses SidI-mediated inhibition of protein translation. SidI binding to eEF1A and Lpg2505 is not mutually exclusive, and the proteins bind distinct regions of SidI. We also discovered that SidI possesses GDP-dependent glycosyl hydrolase activity and that this activity is regulated by Lpg2505. We have therefore renamed Lpg2505 MesI (metaeffector of SidI). This work reveals novel enzymatic activity for SidI and provides insight into how intracellular replication of L. pneumophila is regulated by a metaeffector.

Phosphoglycerate kinase 1 (PGK1) plays important roles in glycolysis, yet its forward reaction kinetics are unknown, and its role especially in regulating cancer cell glycolysis is unclear. Here, we developed an enzyme assay to measure the kinetic parameters of the PGK1-catalyzed forward reaction. The Km values for 1,3-bisphosphoglyceric acid (1,3-BPG, the forward reaction substrate) were 4.36 μm (yeast PGK1) and 6.86 μm (human PKG1). The Km values for 3-phosphoglycerate (3-PG, the reverse reaction substrate and a serine precursor) were 146 μm (yeast PGK1) and 186 μm (human PGK1). The Vmax of the forward reaction was about 3.5- and 5.8-fold higher than that of the reverse reaction for the human and yeast enzymes, respectively. Consistently, the intracellular steady-state concentrations of 3-PG were between 180 and 550 μm in cancer cells, providing a basis for glycolysis to shuttle 3-PG to the serine synthesis pathway. Using siRNA-mediated PGK1-specific knockdown in five cancer cell lines derived from different tissues, along with titration of PGK1 in a cell-free glycolysis system, we found that the perturbation of PGK1 had no effect or only marginal effects on the glucose consumption and lactate generation. The PGK1 knockdown increased the concentrations of fructose 1,6-bisphosphate, dihydroxyacetone phosphate, glyceraldehyde 3-phosphate, and 1,3-BPG in nearly equal proportions, controlled by the kinetic and thermodynamic states of glycolysis. We conclude that perturbation of PGK1 in cancer cells insignificantly affects the conversion of glucose to lactate in glycolysis.

Prof. Jason Pomeroy talks about the rise of the digital economy and its impact on the workplace.

तनाव देख पुलिस तैनात, पीएम के बाद आएगा शव

नगर कोतवाली इलाके के दहिलामऊ स्थित चांदमारी मारुतनगर में सई नदी के किनारे विवादित जमीन पर प्लाटिंग और रुपये के विवाद को लेकर दो गुटों में शुक्रवार दोपहर भिड़ंत हो गई। दोनों पक्षों से दर्जनों राउंड फायरिंग हुई। गोलियों की तड़तड़ाहट से लोग सहम गए।

Publisher 2K Games and The Golf Club developer HB Studios have announced PGA Tour 2K21 and released a teaser trailer for the game. 

Platforms and a release date were not announced, however, more information on the game will be released on May 14. Visit the official website here.

View the teaser trailer below:

A life-long and avid gamer, William D'Angelo was first introduced to VGChartz in 2007. After years of supporting the site, he was brought on in 2010 as a junior analyst, working his way up to lead analyst in 2012. He has expanded his involvement in the gaming community by producing content on his own YouTube channel and Twitch channel dedicated to gaming Let's Plays and tutorials. You can contact the author at wdangelo@vgchartz.com or on Twitter @TrunksWD.

Full Article - https://www.vgchartz.com/article/443403/pga-tour-2k21-announced-more-information-coming-may-14/

Bandai Namco has announced a new RPG, Scarlet Nexus, for the Xbox Series X and Xbox One. It will feature Smart Delivery. A release date was not announced. 

"The next generation of video game home consoles lets the development team realize the full potential of Scarlet Nexus," said producer Keita Iizuka. "Players can expect visuals and psychic battles with high resolution and frame rate along with fully interactive real-time battles with dynamic animation. Utilizing Smart Delivery on Xbox Series X also means players can enjoy the best version of Scarlet Nexus regardless of whether they are playing on the next generation with Xbox Series X or Xbox One."

Bandai Namco America senior brand manager Stephen Akana added, "Scarlet Nexus aims to change the way gamers view Japanese RPGs with a powerful futuristic aesthetic as well as a streamlined combat system that balances fast-paced action with strategic planning. Lead by an extremely talented team at Bandai Namco Studios, including members from fan-favorite title Tales of Vesperia, Scarlet Nexus combines years of game development experience with the excitement and creative freedom of the power behind the next generation of home console systems."

View the announcement trailer below:

Here is an overview of the game:

Scarlet Nexus takes place in the far distant future, where a psionic hormone was discovered in the human brain, granting people extra-sensory powers and changed the world as we knew it. As humanity entered this new era, deranged mutants known as Others began to descend from the sky with a hunger for human brains. Impervious to conventional weapons, those with acute extra-sensory abilities, known as psionics, were our only chance to fight the onslaught from above and preserve humanity. Since then, psionics have been scouted for their talents and recruited to the Other Suppression Force, humanity’s last line of defense.

In Scarlet Nexus, players will take on the role of Yuito Sumeragi, armed with a talent in psycho-kinesis, and explore the futuristic city of New Himuka and uncover the mysteries of a Brain Punk future caught between technology and physic abilities. With these psycho-kinetic powers, the world becomes an important ally. Lift, break, and hurl pieces of the environment to build attack combos and lay waste to enemies.

A life-long and avid gamer, William D'Angelo was first introduced to VGChartz in 2007. After years of supporting the site, he was brought on in 2010 as a junior analyst, working his way up to lead analyst in 2012. He has expanded his involvement in the gaming community by producing content on his own YouTube channel and Twitch channel dedicated to gaming Let's Plays and tutorials. You can contact the author at wdangelo@vgchartz.com or on Twitter @TrunksWD.

Full Article - https://www.vgchartz.com/article/443425/bandai-namco-announces-rpg-scarlet-nexus-for-xbox-series-x-and-xbox-one/

The Ascent is a new solo and co-op action RPG set in a cyberpunk world from developer Neon Giant and publisher Curve Digital. It was announced today during Microsoft's Inside Xbox showcase for the Xbox Series X and Xbox One. It will feature Smart Delivery. The Ascent will launch in Holiday 2020.

View the reveal trailer below:

Here is an overview of the game:

The Ascent is a solo and co-op action RPG set in a cyberpunk world. The mega corporation that owns you and everyone, The Ascent Group, has just collapsed. Confusion and chaos ensue, security and order are in disarray, and without protection, everyone is left to fend for themselves. Stop gangs and hostile corporations from taking over and discover what really happened.

Explore the Ascent Group arcology

Make allies as well as enemies as you explore the brimming arcology and its wide range of districts, from the deep slums to the higher luxury spheres.

Shoot and Loot

Consider enemies' height and weaknesses into account when fighting, making the most out of every opportunity. Take down even the worst opponents to find the rarest loot.

Customization and Augmentations

Modify your body with cyberware and gear up with weapons and equipment that suit your playstyle. Take a slick and precise approach or arm yourself to the teeth and go full chrome.

Solo or Co-op

Play the entire game alone or work together with up to three friends in local or online co-op.

A life-long and avid gamer, William D'Angelo was first introduced to VGChartz in 2007. After years of supporting the site, he was brought on in 2010 as a junior analyst, working his way up to lead analyst in 2012. He has expanded his involvement in the gaming community by producing content on his own YouTube channel and Twitch channel dedicated to gaming Let's Plays and tutorials. You can contact the author at wdangelo@vgchartz.com or on Twitter @TrunksWD.

Full Article - https://www.vgchartz.com/article/443427/the-ascent-is-a-cyberpunk-action-rpg-announced-for-xbox-series-x-and-xbox-one/

Google has been folding in a score of updates to help delineate Duo from the dozens of other video chat services available.

पोस्टग्रेजुएट इंस्टीट्यूट ऑफ मेडिकल एजुकेशन एंड रिसर्च (PGIMER), चंडीगढ़ में अनेक पदों पर भर्तियां होने जा रही हैं।

The improvements include nimbler feet, better perception, and fully custom electronics

Laharl is coming back for another go. The Old School RPG was apparently too old school. And Charizard is burning up his own 3DS. All...

A few games with sordid histories are getting second chances. Skyrim is getting another collector’s edition. Baldur’s Gate is getting a new release date. Unfortunately,...

This week we figure out BioWare’s next title. Then we settle the great question of Persona 4 vs. Nocturne. Finally, we give our list of...

You’re so cool, baby. You know you’re the best at talking to demons in SMT IV. You know you’re gonna get that Pokédex on the...

This week we figure out what to play on Wii U. Pier Solar decides what we should play on the Dreamcast. FFXIV decides that we...

Kate Middleton is pregnant! I mean sure, there’s news about a successor to Planescape: Torment. And yeah there’s potentially a new Star Ocean game. But...

We discover the most appropriate analogy for the current handheld market. Jon decides he’s ready to import a 3DS. Square Enix and Nintendo demonstrate how...

In this very special episode of the RPGCast, there’s a Christmas miracle! That’s right, we come up with a useful idea for the Wii U...

RPG Cast is back from its short hiatus with a couple hours of RPG related fun! CES brought us new gadgets to be cynical about....

This was a week of impossible announcements. Shin Megami Tensei crosses over with Fire Emblem. Project X Zone is coming to North America. Earthbound hits...

Star Trek Online is celebrating its third anniversary, but we uncover the sordid coverup that lies between the game’s aft nacelles. Also, you can now...

Class of Heroes decides to do a physical version after all. The Big Huge Games folks get more bad news. Quin teaches us about fictional...

Today Anna talks about her maple addiction and gets counseling to break the cycle. Meanwhile Manny gets the help he needs to conquer his fears...

You don’t need fractions, don’t take square roots. Don’t need no matrix math to compute this product. It’s small then it’s bigger then it’s huge...

EA loves microtransactions. Anna loves farming sims. Square Enix loves trademarks. Chris loves pirates. Mac addicts love…well…any game ports. Manny loves Devil Summoner. Zenimax loves...

This week Ryan Baker from Pixelscopic joins us to talk about their kickstarted game, Delver’s Drop. Also, EA does a micro back transaction, Eidos makes...

Bethesda challenges our hipster fashion instincts. Nintendo is doing something with that NFC sensor. Square Enix is faring pretty well, actually. And then there’s EA…who...

Chris uncovers a human revolution happening on the Wii U and a coded princess debuting in Europe. Anna finds a dragon’s crown somewhere in the...

Microsoft tries out a new PR strategy. Armored Core gets the death metal mix it’s always needed. Torchlight 2 shows its GUTS. Ew.

All our hopes and dreams for the next generation of consoles now have a fitting container! Aside from that, Mewtwo misplaces his tail and it...

There’s peanut butter in my chocolate in this week’s RPG Cast. We figure out how to solve the JRPG problem. Nintendo announces every RPG ever....

Between artwork controversies and some backwash from a riptide, we find a way to bring you the biggest news of the week. You won’t believe...

This week we learn what everyone is looking forward to playing in 2013. We also find a new way to get our Game Dev Story...

What a week. First we learn what really happens at E3. Then Quin gets hungry. After that, Square Enix gets real specific. But Diablo 3...

People overreact to layoffs. Candy Box consumes another person’s life. Square Enix decides the best thing for its future is to make an HD version...

Manny learns about gaming chairs. Chris learns about digestives. Jon learns about robot physics. Anna Marie learns about playing 30 Final Fantasy XIV characters simultaneously....

Warden Chris has left the building, now the inmates are running the asylum. Hear want these irregulars have to say about the news of this...

Hear about the RPGamer wedding of the decade, the honeymoon, oh and some show called E3. Manny and Scott join Anna Marie and Chris as...

We cut our way to the main news…oh look another trading card. I mean…wow that’s a good price for that game…er…excuse me. We do a...

Chris gets his wish, but is it more than he can handle? Eidos’s GM decides that yes, it is more than he can handle. The...

The internet conspires against the podcast this week. First it sends cats to attack the equipment, then it breaks Twitch.tv, and finally it takes out...

Podcasting. Podcasting never changes. Kind of like Nintendo’s 3rd party support. Or perhaps the game market for OSX. Also, let’s be real, Pokémon may have...

The saviors of the JRPG genre are here! All backers receive early access to the alpha of Jon Yearworth Plays Some Mech Games. $10,000 donators...