The U.S. Department of Health and Human Services (HHS) Office of the Assistant Secretary for Health (OASH) should focus funding on holistic, evidence-based, population-wide adolescent health programs that consider adolescent risk-taking as normative, according to a new report from the National Academies of Sciences, Engineering, and Medicine.

In light of the present situation in the U.S., we believe that it is essential to explore a wide range of options for treating the increasing numbers of very ill patients with COVID-19 respiratory illness.

Adolescents need to be heard to ensure that health programs that serve them are reaching them effectively, according to Promoting Positive Adolescent Health Behaviors and Outcomes, a report released last December by the National Academies.

As millions of adolescents head back to school, what can science tell us about how the isolation and upheaval of the past year-and-a-half have affected their mental health and development?

Explore the art of natural perfumery at the UC Botanical Garden at Berkeley with Jessica Mennella of FLWR Napa Valley. Learn the foundation and techniques of natural perfumery using the Slow Scent® method of perfuming developed by renowned perfumer Mandy Aftel. Jessica Mennella, trained by Aftel, will be your instructor for this hands-on and interactive class. During the workshop, you will have the opportunity to explore 18 botanicals whose complexity of scent is linked directly to the plants and flowers from which they are derived. You will also learn the basic structure of Slow Scent® natural perfume formulation and blending techniques. No prior experience is necessary, and all materials will be provided. By the end of the workshop, you will have created your own signature perfume using the formula you developed in class. Additionally, you will receive a guide to the process of making natural perfumes. The art of natural perfumery is rooted in the history and beauty of botanical materials. And among our senses, scent has the unique ability to instantly transform our mood and life experience. Come perfume in the gardens and discover for yourself how scent can take you to another place and time. Whether you are new to natural perfumery or have attended previous workshops, you are welcome to join us!

Millions of adolescents in Bangladesh will be able to access health information and services through two online portals launched at the National Adolescent Health Conference.

To Address the Escalating Need for Substance Use Disorder Treatment, Boulder Announces New Services Available to UnitedHealthcare Members under 18.

FDA-cleared depression treatment is a non-invasive procedure that does not require sedation or drugs, and has minimal to no side effects.

Your personal-use humidifier. Bliz provides moisture to the air by delivering a cool, refreshing flavored mist no matter where you are

Research Highlights: In the first study, nearly 23% of young adults (ages 18-39 years) included in the NHANES 2017-2020 datasets had high blood pressure (130/80 mm Hg or greater). In addition, they were more likely to self-report being uninsured, food ...

Sylvain Sarrailh is a French illustrator and concept artist with over a decade of experience in the video game and film industries. He has collaborated with prominent companies such as Ubisoft, Rocksteady, Amplitude, Psyop, Oculus, Insomniac Games, Sony Pictures, and DreamWorks. Sarrailh’s portfolio showcases a diverse range of vibrant and detailed artworks, reflecting his versatility […]

Australia is a nation built on migration, with many citizens tracing their heritage back to different parts of the world. For those born outside Australia to Australian parents, citizenship by descent offers a direct route to becoming an Australian citizen. What is Citizenship by Descent? Citizenship by descent is a way for individuals born outside […]

The post Becoming an Australian Citizen by Descent – A Path for Those Born Abroad appeared first on Australian Visa Experts.

Toronto, ON – Today, the John H. Daniels Faculty of Architecture, Landscape, and Design celebrated the official opening of its new home — the Daniels Building — at historic One Spadina Crescent. Located on the western edge of the University of Toronto’s St. George campus just north of College Street, the iconic neo-gothic building and […]

Crescent Bay by Kerry Hallam is a(n) Limited Edition. The Edition is Limited to pcs

Crescent Bay Canvas by Kerry Hallam is a(n) Limited Edition. The Edition is Limited to pcs

The Ascent-acrylic by Christopher Pardell is a(n) Limited Edition. The Edition is Limited to Limited Edition of 950 pcs

The Ascent-acrylic by Christopher Pardell is a(n) Limited Edition. The Edition is Limited to Limited Edition of 950 pcs

An anonymous reader shares a report: Tucked inside a 32-page earnings report, oil and gas giant BP revealed it was killing 18 early-stage hydrogen projects, a move that could have a chilling effect on the nascent hydrogen industry. The decision, along with the sale of the company's U.S. on-shore wind power operations, will save BP $200 million annually and help boost its bottom line. The hydrogen industry, which has relied on oil and gas companies both financially and through lobbying efforts, is preparing for a grimmer outcome. BP has been a supporter of hydrogen. The company's venture capital arm has invested in several green hydrogen startups, including Electric Hydrogen and Advanced Ionics. Earlier this year, BP said it would develop "more than 10" hydrogen projects in the U.S., Europe, and Australia. Now, BP is scaling back those plans, saying it'll develop between five and ten projects. The company is keeping quiet about which ones will receive the green light.

Read more of this story at Slashdot.

El adolescente perpetró el asesinato mientras su hermano de 13 años sujetaba a Silvia L.G. que junto a su marido los había adoptado siendo niños. Después de acuchillarla, el homicida quitó la ropa a la víctima y "con ánimo libidinoso le introdujo varias veces los dedos en la vagina" Leer

Location: Electronic Resource- 

Ultra-fine scented cotton seamless footcovers. Lowcut for a fashionable look. 2 pair pack. Nude color only. Propeds Footcovers knitted with ultra-fine scented cotton blended with spandex and nylon to give you a comfortable fit, with a lowcut for a fashionable look. They wick moisture away from the feet, keep your feet fresh and prolong the lie of shoes. Nude color only. Sizes S,M,L,Q. See Sizechart. Price: USD5.28

Please share with me your favorite places online to order custom die-cut vinyl stickers, preferably iridescent or glitter or iridescent glitter, using art I provide. I'd ideally like these to be about 2 square inches, and really shiny or sparkly, but with good (outdoor-quality) staying power. I have a few different designs I'd like to have made, in quantities of about 55 each. I put in a bid with diecutstickers.com almost a month ago now and never heard back beyond the initial form response, so I'm looking for other options for quality work with a fast turnaround time. Thank you!

Expertos analizaron el panorama fiscal del departamento, los logros de los últimos años y los desafíos que se tiene de cara a la búsqueda de mayor descentralización.

El recién llegado alcalde de Cartagena habló de las intervenciones en el centro histórico de la ciudad y de las acciones que vienen para recupera la infraestructura en la zona.

Panelistas analizaron los riesgos y beneficios que trae el aumento de transferencias de la nación a las regiones, así como el aumento de competencias y responsabilidades a las regiones.

Desde bebés hasta adolescentes es importante cuidar la piel.

La psicóloga y máster en psicología clínica conductiva, Carolina Villanueva, compartió algunas señalas de alarma que debería tener presente

En Familia, discutimos las implicaciones de utilizar imágenes de niños en publicidad.

En Familia, le contamos cuál es la importancia de que Colombia cuente con una ley que regule el uso y venta de vapeadores y cigarrillos electrónicos.

What Danielle Smith posted on her subscribers-only social media; how litter boxes in schools became a Republican talking point; Yankee scented candle reviews as COVID indicator; a surgeon struggles to care for patients through Ethiopia's civil war; Brent Bambury returns and more.

An incoherent album from the grime godfather, but one full of loveable eccentricities.

Nascent Group Ltd. announced that it has signed an agreement to acquire Bermuda Compliance Consultants Ltd, and the acquisition is expected to close in the fourth quarter of 2023. A spokesperson said, “Founded and headquartered in Bermuda, Bermuda Compliance Consultants Ltd. [BCCL] provides compliance advisory services to the insurance and the fund markets. Alison Morrison, […]

By JM McNab Published: November 12th, 2024

Ileana M. Cristea
Dec 1, 2005; 4:1933-1941
Research

Ulrich Rothbauer
Feb 1, 2008; 7:282-289
Research

Rhodopsin is a canonical class A photosensitive G protein–coupled receptor (GPCR), yet relatively few pharmaceutical agents targeting this visual receptor have been identified, in part due to the unique characteristics of its light-sensitive, covalently bound retinal ligands. Rhodopsin becomes activated when light isomerizes 11-cis-retinal into an agonist, all-trans-retinal (ATR), which enables the receptor to activate its G protein. We have previously demonstrated that, despite being covalently bound, ATR can display properties of equilibrium binding, yet how this is accomplished is unknown. Here, we describe a new approach for both identifying compounds that can activate and attenuate rhodopsin and testing the hypothesis that opsin binds retinal in equilibrium. Our method uses opsin-based fluorescent sensors, which directly report the formation of active receptor conformations by detecting the binding of G protein or arrestin fragments that have been fused onto the receptor's C terminus. We show that these biosensors can be used to monitor equilibrium binding of the agonist, ATR, as well as the noncovalent binding of β-ionone, an antagonist for G protein activation. Finally, we use these novel biosensors to observe ATR release from an activated, unlabeled receptor and its subsequent transfer to the sensor in real time. Taken together, these data support the retinal equilibrium binding hypothesis. The approach we describe should prove directly translatable to other GPCRs, providing a new tool for ligand discovery and mutant characterization.

No abstract

DNA polymerase from bacteriophage T7 undergoes large, substrate-induced conformational changes that are thought to account for high replication fidelity, but prior studies were adversely affected by mutations required to construct a Cys-lite variant needed for site-specific fluorescence labeling. Here we have optimized the direct incorporation of a fluorescent un-natural amino acid, (7-hydroxy-4-coumarin-yl)-ethylglycine, using orthogonal amber suppression machinery in Escherichia coli. MS methods verify that the unnatural amino acid is only incorporated at one position with minimal background. We show that the single fluorophore provides a signal to detect nucleotide-induced conformational changes through equilibrium and stopped-flow kinetic measurements of correct nucleotide binding and incorporation. Pre-steady-state chemical quench methods show that the kinetics and fidelity of DNA replication catalyzed by the labeled enzyme are largely unaffected by the unnatural amino acid. These advances enable rigorous analysis to establish the kinetic and mechanistic basis for high-fidelity DNA replication.

Mosquito-borne disease is a major global public health issue. One path toward the development of evidence-based strategies to limit mosquito biting is the study of the mosquito nervous system—in particular, the sensory systems that drive biting behavior. The central nervous system of insects consists of the brain and the ventral nerve cord. Here, we describe a protocol for dissecting, immunofluorescent labeling, and imaging both of these structures in the mosquito. This protocol was optimized for Aedes aegypti and works well on Anopheles gambiae tissue. It has not been tested in other mosquito species, but we anticipate that it would work on a range of mosquitoes, and, if not, our protocol will provide a starting point from which to optimize. Notably, a limited number of antibodies cross-react with Ae. aegypti proteins. This protocol is intended for use with validated antibodies and can also be used to test new antibodies as they are generated. It has been successfully used to visualize protein tags, such as green fluorescent protein, that have been introduced into the mosquito to amplify or detect their presence.

In insects, oocyte resorption (oosorption) or follicular atresia is one of the key physiological processes and evolutionary strategies used to optimize reproductive fitness. Mosquitoes are ideal model organisms for studying egg maturation in arthropods, as their follicle development is initiated only following the ingestion of a blood meal, followed by a carefully orchestrated series of hormonally regulated events leading to egg maturation. A cohort of approximately 100 follicles per mosquito ovary begin developing synchronously. However, a significant fraction of follicles ultimately undergo apoptosis and oosorption, especially when available resources from the blood meal are limited. Therefore, simple, rapid, and reliable techniques to accurately evaluate follicular atresia are necessary to understand mechanisms underlying follicle development in insects. This protocol describes how to detect apoptotic follicle cells within the Aedes aegypti mosquito ovaries using a commercially available fluorescent-labeled inhibitor of caspases (FLICA). Caspases are key players in animal apoptosis. In this assay, the FLICA reagent enters the intracellular compartment of follicles in dissected mosquito ovaries and covalently binds to active caspases. The bound reagent remains within the cell and its fluorescent signal can be observed by confocal microscopy. Although this method was specifically developed for visualizing apoptotic ovarian follicles during Ae. aegypti mosquito egg development, it should be applicable to other mosquito tissues that undergo caspase-mediated program cell death in a time-dependent manner.

Allegro X System Capture offers a complete ecosystem for library development. This post introduces the latest DE-HDL Library Development using System Capture course in which you learn how to create different library objects. As a librarian, you often work with numerous libraries. Your tasks include creating or modifying symbols for libraries. To use Allegro X System Capture to create a library, you can follow the steps in the following flowchart: Let’s go through each step in detail. Setting the CDS_SITE Variable Before you start library development for a new project, set the CDS_SITE system environment variable. This step is required to access libraries and other configuration files. Creating a Project in Allegro X System Capture The next step is to create a project in Allegro X System Capture. Adding a Library to the Project Symbol development consists of creating symbol graphics, electrical data, and properties used by different tools in the PCB design flow. To add a library to a project, first create a library in the Libraries pane of the Project e xplorer. Creating Library Symbols The library development process supports the creation of various types of symbols. Creating a Symbol with Multiple Views You can generate multiple views of the same symbol using the Duplicate command. For example, a discrete symbol, such as a resistor, can have multiple views, as shown in the following image: Creating a Split Symbol For advanced designs, you often need to create library symbols and break them into multiple sections to support the design process. When a symbol shows all the logical pins in the physical package, it is called a single-section or flat symbol. Many large ICs have several pins and the symbols need to fit on a single schematic page. One workaround is to use vector pin names on a symbol to reduce its size, although manufacturers prefer schematics that show each pin. You can divide these high-pin count devices into smaller pieces, where each piece is a separate version of the part. Such parts are referred to as split parts or multi-section symbols. For multi-section symbols, you can create two types of split parts—symmetrical and asymmetrical. Symmetrical Split Symbols A symmetrical split symbol has only one symbol graphic, which holds two or more identical logic symbols, each with its own unique physical pin numbers. You can create a symmetrical split symbol using the Duplicate Section icon in the canvas window. Each symbol section contains the same set of pins but different pin numbers, as shown in the following image: Asymmetrical Split Symbols An asymmetrical split symbol is a symbol whose physical package contains one or more unique schematic symbols. You can create an asymmetrical split symbol by clicking the New Section icon in the canvas window. Asymmetrical symbols have a unique set of logical pins, as shown in the following image: Creating Symbols Using the Spreadsheet Interface To simplify the development of large symbols, Allegro X System Capture has a Spreadsheet Interface . You can copy from a spreadsheet into the interface. This saves time and helps minimize errors introduced by manual entry. In conclusion, the DE-HDL library development using Allegro X System Capture course involves several critical steps and supports various symbol creation techniques. This course helps librarians create and modify symbols effortlessly and deepens their understanding of library development within Allegro X System Capture. To learn more about this topic, enroll in the DE-HDL Library Development using Allegro X System Capture course on the Cadence Support portal . Click the training byte link now or visit Cadence Support and search for training bytes under Video Library. If you find the post useful and want to delve deeper into training details, enroll in the following online training course for lab instructions and a downloadable design: DE-HDL Library Development using Allegro X System Capture (Online). You can become Cadence Certified once you complete the course. Cadence Training Services now offers free Digital Badges for all popular online training courses. These badges indicate proficiency in a certain technology or skill and give you a way to validate your expertise to managers and potential employers. You can add the digital badge to your email signature or any social media channels, such as Facebook or LinkedIn, to highlight your expertise. To find out more, see the blog post Take a Cadence Masterclass and Get a Badge . You might also be interested in the training Learning Map that guides you through recommended course flows as well as tool experience and knowledge-level training modules. To find information on how to get an account on the Cadence Learning and Support portal, see here . SUBSCRIBE to the Cadence training newsletter to be updated about upcoming training, webinars, and much more. If you have any questions about courses, schedules, online training, blended/virtual live training, or public, or onsite live training, reach out to us at Cadence Training .

Title: No Change in Adolescent Drug, Alcohol Use During Pandemic
Category: Health News
Created: 8/24/2021 12:00:00 AM
Last Editorial Review: 8/24/2021 12:00:00 AM

ABSTRACTIntroduction:Family Planning 2020 (FP2020) was established in 2012 with the goal of expanding contraceptive access. By 2020, 46 countries had made commitments to FP2020. A sustained focus on adolescents and youth (AY) began in 2016. During the commitment formulation process, substantial support was offered to countries to develop AY commitments based on sound data, research evidence, and programmatic experience. This study assesses how country commitments under FP2020 and FP2030 have evolved over time with respect to improving attention to and focus on the needs of adolescents and youth sexual and reproductive health (AYSRH).Methods:We analyzed the content of FP2020 and FP2030 country commitments focusing on AY (aged 10–24 years) using a scoring guideline we developed to measure the AY commitments in terms of completeness, clarity, and quality.Results:This analysis shows that FP2030 commitments better articulate strategies and activities to reach AY with contraceptive information and services when compared to FP2020 commitments.Conclusion:FP2030 commitments are stronger in some areas on AYSRH, such as commitment to establish national or local policies, strategies, and guidance for AY programming, specifying the target audience of the AY commitment, and partnering with AY or youth-led organizations in commitments. However, more work remains to be done by countries to dedicate a budget for achieving AY objectives, including measurable targets for monitoring progress, identifying and addressing the root causes that impact AY access to and use of contraception, including child marriage and gender-based violence, and reducing financial barriers to access contraception.