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Separation and identification of permethylated glycan isomers by reversed phase nanoLC-NSI-MS

Simone Kurz
Dec 29, 2020; 0:RA120.002266v1-mcp.RA120.002266
Research




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Separation and identification of permethylated glycan isomers by reversed phase nanoLC-NSI-MS [Research]

High performance liquid chromatography has been employed for decades to enhance detection sensitivity and quantification of complex analytes within biological mixtures. Among these analytes, glycans released from glycoproteins and glycolipids have been characterized as underivatized or fluorescently tagged derivatives by HPLC coupled to various detection methods. These approaches have proven extremely useful for profiling the structural diversity of glycoprotein and glycolipid glycosylation but require the availability of glycan standards and secondary orthogonal degradation strategies to validate structural assignments. A robust method for HPLC separation of glycans as their permethylated derivatives, coupled with in-line MSn fragmentation to assign structural features independent of standards, would significantly enhance the depth of knowledge obtainable from biological samples. Here, we report an optimized workflow for LC-MS analysis of permethylated glycans that includes sample preparation, mobile phase optimization, and MSn method development to resolve structural isomers on-the-fly. We report baseline separation and MSn fragmentation of isomeric N- and O-glycan structures, aided by supplementing mobile phases with Li+, which simplifies adduct heterogeneity and facilitates cross-ring fragmentation to obtain valuable monosaccharide linkage information. Our workflow has been adapted from standard proteomics-based workflows and, therefore, provides opportunities for laboratories with expertise in proteomics to acquire glycomic data with minimal deviation from existing buffer systems, chromatography media, and instrument configurations. Furthermore, our workflow does not require a mass spectrometer with high-resolution/accurate mass capabilities. The rapidly evolving appreciation of the biological significance of glycans for human health and disease requires the implementation of high-throughput methods to identify and quantify glycans harvested from sample sets of sufficient size to achieve appropriately powered statistical significance. The LC-MSn approach we report generates glycan isomeric separations, robust structural characterization, and is amenable to auto-sampling with associated throughput enhancements.




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My District Reversed Course on the Implicit Bias Training We Need. What Now?

The principal advice column takes on communicating district decisions you disagree with, optimizing virtual lunch bunches, and more.




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World-First Stem Cell Treatment Reverses Diabetes for a Patient in China, Study Suggests

Scientists converted the patient’s own cells into blood sugar-regulating cell clusters before injecting them back into her abdomen—and one year later, she still doesn't need insulin injections




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AG Jennings calls on Barr to reverse new policy that “will erode the public’s confidence in the 2020 election”

Attorney General Jennings today called on U.S. Attorney General William Barr to reverse his abrupt change to a 40-year-old U.S. Department of Justice policy that until this week had kept the department from interfering with election results. In a letter to AG Barr, AG Jennings and 22 other attorneys general strongly objected to a November […]



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Covid-19 likely to reverse Middle East consulting gains

Consulting firms in the Middle East are likely to take a hit in 2020 due to the coronavirus, after two strong years.




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9th day of reverse Russia-Euro gas flow

The Yamal-Europe pipeline that usually delivers Russian gas to Western Europe was sending the fuel back to Poland for a ninth straight day yesterday, as per data from German network operator Gascade.




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Reps ask NPA to reverse termination of agreement with Intels

The House of Representatives yesterday waded into the ongoing controversy between the Nigeria Ports Authority (NPA) and Intels Nigeria Limited. The House, while adopting a motion by Rep Diri Douye (PDP, Bayelsa), resolved to constitute an ad hoc panel to probe the circumstances that led to NPA’s decision to terminate an existing contract between it […]

Reps ask NPA to reverse termination of agreement with Intels




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Can we finally reverse balding with these new experimental treatments?

Male pattern baldness could soon be a thing of the past, with new hair loss treatments beginning to show tantalising results




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Concussion Recovery Can Reverse After Return to Activity, Study Shows

Title: Concussion Recovery Can Reverse After Return to Activity, Study Shows
Category: Health News
Created: 8/28/2014 9:36:00 AM
Last Editorial Review: 8/28/2014 12:00:00 AM




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Mouse Study Suggests Stem Cells May Reverse Stroke Damage

Title: Mouse Study Suggests Stem Cells May Reverse Stroke Damage
Category: Health News
Created: 8/22/2016 12:00:00 AM
Last Editorial Review: 8/23/2016 12:00:00 AM




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KLS-13019, a Novel Structural Analogue of Cannabidiol and GPR55 Receptor Antagonist, Prevents and Reverses Chemotherapy-Induced Peripheral Neuropathy in Rats [Special Section: Cannabinoid Signaling in Human Health and Disease]

Neuropathic pain is a form of chronic pain that develops because of damage to the nervous system. Treatment of neuropathic pain is often incompletely effective, and most available therapeutics have only moderate efficacy and present side effects that limit their use. Opioids are commonly prescribed for the management of neuropathic pain despite equivocal results in clinical studies and significant abuse potential. Thus, neuropathic pain represents an area of critical unmet medical need, and novel classes of therapeutics with improved efficacy and safety profiles are urgently needed. The cannabidiol structural analog and novel antagonist of GPR55, KLS-13019, was screened in rat models of neuropathic pain. Tactile sensitivity associated with chemotherapy exposure was induced in rats with once-daily 1-mg/kg paclitaxel injections for 4 days or 5 mg/kg oxaliplatin every third day for 1 week. Rats were then administered KLS-13019 or comparator drugs on day 7 in an acute dosing paradigm or days 7–10 in a chronic dosing paradigm, and mechanical or cold allodynia was assessed. Allodynia was reversed in a dose-dependent manner in the rats treated with KLS-13019, with the highest dose reverting the response to prepaclitaxel injection baseline levels with both intraperitoneal and oral administration after acute dosing. In the chronic dosing paradigm, four consecutive doses of KLS-13019 completely reversed allodynia for the duration of the phenotype in control animals. Additionally, coadministration of KLS-13019 with paclitaxel prevented the allodynic phenotype from developing. Together, these data suggest that KLS-13019 represents a potential new drug for the treatment of neuropathic pain.

SIGNIFICANCE STATEMENT

Chemotherapy-induced peripheral neuropathy (CIPN) is a common, debilitating side effect of cancer treatment with no known cure. The GPR55 antagonist KLS-13019 represents a novel class of drug for this condition that is a potent, durable inhibitor of allodynia associated with CIPN in rats in both prevention and reversal-dosing paradigms. This novel therapeutic approach addresses a critical area of unmet medical need.




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Trump Will Reverse Biden's Israel Delusions

Donald Trump will embrace the truth Joe Biden has refused to countenance: Israel's enemies are America's enemies. And when Israel defeats its enemies, America wins.




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Can we finally reverse balding with these new experimental treatments?

Male pattern baldness could soon be a thing of the past, with new hair loss treatments beginning to show tantalising results




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What Trump Can—And Probably Can’t—Do to Reverse U.S. Climate Policy

The new president-elect can go beyond just pulling out of the Paris Agreement. But it may be more difficult to roll back clean energy policies




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Tax-News.com: Switzerland To Introduce VAT Domestic Reverse Charge On Emissions Trading

Switzerland has proposed introducing the value-added tax reverse charge mechanism for domestic transactions in emission allowances.




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Tax-News.com: Irish VAT Rate Cut To Be Reversed In March

The Irish headline rate of VAT will return to 23 percent from March, having been temporarily reduced in response to the COVID-19 pandemic.




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Tax-News.com: UK Releases Construction VAT Reverse Charge Guidance

On September 24, 2020, HM Revenue and Customs released in-depth guidance on the introduction of the VAT reverse charge mechanism on the supply of building or construction services.




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X reverses course in Brazil

It seems that Elon Musk-owned social network X (formerly Twitter) is backing down from a confrontation with Brazil’s Supreme Court. The New York Times reported on a new court filing in which the company’s lawyers said X had complied with the court’s orders — blocking designated accounts, paying fines, and naming a new formal representative […]

© 2024 TechCrunch. All rights reserved. For personal use only.




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A Medical Milestone : Scientists Transform Fat Cells to Reverse Type 1 Diabetes

Living with chronic illness can significantly impact daily life, forcing individuals to constantly adapt to their condition. For those with type 1 diabetes, managing blood sugar levels is a daily challenge that requires diligence and effort. Fortunately, recent scientific advancements are




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Experimental and simulation study of reverse micelles formed by aerosol-OT and water in non-polar solvents

Phys. Chem. Chem. Phys., 2024, 26,27772-27782
DOI: 10.1039/D4CP03389B, Paper
Open Access
  This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.
Angie Mat'usová, Georgina Moody, Peter J. Dowding, Julian Eastoe, Philip J. Camp
Small-angle neutron scattering and molecular dynamics simulations are used to characterise aerosol-OT reverse micelles. Simulations capture spontaneous self-assembly, compare favourably with experiments, and give microscopic information.
The content of this RSS Feed (c) The Royal Society of Chemistry




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GST payers get communication to first show irreversible ITC and then reverse, experts advise caution in filing Nov GSTR-3B

In August 2022 this year, Central Board of Indirect Taxes & Custom (CBIC) issued a detailed clarification on reporting of information about reversal of ITC as well as ineligible ITC




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How to Fix: Windows WASD Keys Reversed with Arrow Keys

This past weekend I had the opportunity to be what every father wants, if only for a moment: the “cool dad”. My wife was out of town and my youngest son wanted to play PUBG. I caved in, taught him the basic FPS key binds, and he was having a great time. While he was […]

The post How to Fix: Windows WASD Keys Reversed with Arrow Keys appeared first on David Walsh Blog.




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The Stockholm reverse and the new storm that has hit Indian tennis




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Correction: Solution-processed white OLEDs with power efficiency over 90 lm W−1 by triplet exciton management with a high triplet energy level interfacial exciplex host and a high reverse intersystem crossing rate blue TADF emitter

Mater. Horiz., 2024, 11,1817-1817
DOI: 10.1039/D4MH90021A, Correction
Open Access
  This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.
Liang Chen, Yufei Chang, Song Shi, Shumeng Wang, Lixiang Wang
The content of this RSS Feed (c) The Royal Society of Chemistry




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Reverse Contests [electronic journal].




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Global Chess League: Nihal’s draw stands, SG Pipers’ appeal to reverse result rejected




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Resolving phytosterols in microalgae using offline two-dimensional reversed phase liquid chromatography-supercritical fluid chromatography coupled with quadrupole time-of-flight mass spectrometry

Anal. Methods, 2024, 16,2278-2285
DOI: 10.1039/D3AY02261G, Paper
Magali Batteau, Elodie Bouju, Adriana Ramirez-Romero, Sylvie Nuccio, René De Vaumas, Florian Delrue, Karine Faure
Development of an off-line LC-SFc-MS method, separation of sterol isomers in algal unsaponifiable fractions, elution pattern helping in the reduction of structural candidates.
The content of this RSS Feed (c) The Royal Society of Chemistry




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575: CSS Errors, Proxy and Reverse Proxy, and What’s The Edge?

Bluesky adds first class support for urls as a username, text-wrap pretty update, sqwunching text update, should CSS spit out errors, anchor functionality, what does the edge mean, eSports and bowling, how to test websites on slower CPUs, and what does proxy or reverse proxy mean?




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Membrane technology for water reuse in decentralised non-sewered sanitation systems: comparison of pressure driven (reverse osmosis) and thermally driven processes (membrane distillation and pervaporation)

Environ. Sci.: Water Res. Technol., 2024, 10,2831-2843
DOI: 10.1039/D4EW00200H, Paper
Open Access
  This article is licensed under a Creative Commons Attribution 3.0 Unported Licence.
E. Mercer, C. Davey, Y. Bajón Fernández, S. Septien, S. Tyrrel, E. Cartmell, M. Pidou, E. J. McAdam
Thermally driven membrane processes provide an alternative method to conventional pressure driven processes to recover high quality water and manage odour from concentrated blackwater, using a low-grade heat source instead of electrical energy.
The content of this RSS Feed (c) The Royal Society of Chemistry




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Concurrent boron removal from reverse osmosis concentrate and energy production using a microbial desalination cell-Donnan dialysis hybrid system

Environ. Sci.: Water Res. Technol., 2024, Advance Article
DOI: 10.1039/D4EW00621F, Paper
A. Yagmur Goren, H. Eser Okten
The removal of boron from aqueous solutions offers an important opportunity to improve the management of sustainable resources.
To cite this article before page numbers are assigned, use the DOI form of citation above.
The content of this RSS Feed (c) The Royal Society of Chemistry




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Kerala University reverses decision on hiring retired hands, opts for contract lecturers instead

During a Syndicate meeting, LDF camp imposes its will through a majority vote in favour of hiring contract lecturers




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Markets reverse early losses, Power Grid leads gains while Asian Paints tumbles

The market recovery came despite a weak opening influenced by China’s stimulus concerns and continued foreign investor selling




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In reverse gear

Terence Lewis says contemporary dance is like soda – it can be added to anything




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Exclusive: Jason Gillespie explains what makes Jasprit Bumrah and Pat Cummins so special, and why reverse swing will be key with Kookaburra ball




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‘Robin Hood in reverse’: the crisis in the Brazilian state

The winner of October’s election will have to address a budget dominated by special interests




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Pause in global college admissions is a chance for India to reverse its student exodus

Convincing migrating students to stay with domestic options post-Covid will require sweeping reforms in the education system




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Sensex, Nifty reverse opening losses, gain over 1% each; what’s driving markets today

HDFC, HDFC Bank, ICICI Bank, Reliance Industries (RIL) and Bharti Airtel were among the top index contributors




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It is time to reverse an unfolding injustice

According to shocking new research by Oxfam, the world’s richest 1% would, on current trends, own more than half the world’s wealth by 2016.




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It is time to reverse an unfolding injustice

According to shocking new research by Oxfam, the world’s richest 1% would, on current trends, own more than half the world’s wealth by 2016.




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Get a free Reverse Trick-or-Treating kit

You pay only shipping for a set of 15 organic, fair trade chocolates and educational cards that your kids can give out to visitors.




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Revolutionary new drug might actually reverse aging

A protein complex has been shown to repair DNA damage caused by radiation or old age.



  • Fitness & Well-Being

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Obama administration reverses 'wild lands' plan

A plan to protect more than 11 million acres of land in the American West has been scrapped after months of talks inside the Beltway.



  • Wilderness & Resources

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Blog: Reverse mentoring – the experiment that got me thinking

Sharon Bishop, CEO at Close Brothers Premium Finance, discusses the lessons that leaders can learn from their staff.




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Video: What is reverse mentoring?

Close Brothers, Sharon Bishop, explains how the management technique is helping her.




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Reverse Mortgage

Before applying one needs to do a lot of home work i.e. research work, that can include talking to a financial experts, going through bank literatures etc.




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Understand Baldness and How You Can Reverse It

Understand hair loss and what treatments can be used to prevent/reverse baldness.




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Reverse Mortgages for Retirees – Boon or Bane?

It’s a commonly quoted statistic that nearly half of American families have no retirement savings. For many people facing retirement, their homes may be the only asset they have to help fund their post career living expenses. Over the last few years there have been a lot of stories about reverse mortgages where retirees sell […]

The post Reverse Mortgages for Retirees – Boon or Bane? appeared first on RSS News Feed.




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Better Laws Can Reverse the Growing Trend of Big Rig Crashes

John Tucker of Kaplan Lawyers PC said that lawmakers can make our roads safer by mandating safer technologies on large trucks.




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California Welcomes "REFORM-A-NATION" Campaign to Reverse Wrongful Convictions and UCLA Law School's Call to End Mass Incarceration

Critically Acclaimed Portrait of Mahatma Gandhi by Anand Jon Alexander featured at University of Southern California "connecting art and law for liberation", featuring actor Danny Glover, Senator Holly Mitchell, and ReStore Justice.