One of the hottest debates in the coronavirus pandemic is whether the malaria drugs promoted as possible treatments by President Donald Trump really work. But Americans don’t seem overly eager to help answer the question. Enrollment in several clinical trials of chloroquine and hydroxychloroquine — including two by the University of Washington — has been […]

According to medical experts, a condition that requires coloured glasses does not exist, yet WA and NSW teachers have attended training sessions on it.

Turner And Boateng Will Oversee The Atlantic A&R Team, Joint Projects, As Well As Maintaining Their Own Artist Rosters

Leeds United's promotion hopes have been thrown into chaos after the coronavirus caused the EFL to suspend all leagues, but the final Championship table has been predicted by a supercomputer. Will Marcelo Bielsa's side finally return to the Premier League, and who will be relegated?

Lens proteins become increasingly cross-linked through nondisulfide linkages during aging and cataract formation. One mechanism that has been implicated in this cross-linking is glycation through formation of advanced glycation end products (AGEs). Here, we found an age-associated increase in stiffness in human lenses that was directly correlated with levels of protein–cross-linking AGEs. α-Crystallin in the lens binds to other proteins and prevents their denaturation and aggregation through its chaperone-like activity. Using a FRET-based assay, we examined the stability of the αA-crystallin–γD-crystallin complex for up to 12 days and observed that this complex is stable in PBS and upon incubation with human lens–epithelial cell lysate or lens homogenate. Addition of 2 mm ATP to the lysate or homogenate did not decrease the stability of the complex. We also generated complexes of human αA-crystallin or αB-crystallin with alcohol dehydrogenase or citrate synthase by applying thermal stress. Upon glycation under physiological conditions, the chaperone–client complexes underwent greater extents of cross-linking than did uncomplexed protein mixtures. LC-MS/MS analyses revealed that the levels of cross-linking AGEs were significantly higher in the glycated chaperone–client complexes than in glycated but uncomplexed protein mixtures. Mouse lenses subjected to thermal stress followed by glycation lost resilience more extensively than lenses subjected to thermal stress or glycation alone, and this loss was accompanied by higher protein cross-linking and higher cross-linking AGE levels. These results uncover a protein cross-linking mechanism in the lens and suggest that AGE-mediated cross-linking of α-crystallin–client complexes could contribute to lens aging and presbyopia.

Lens proteins become increasingly cross-linked through nondisulfide linkages during aging and cataract formation. One mechanism that has been implicated in this cross-linking is glycation through formation of advanced glycation end products (AGEs). Here, we found an age-associated increase in stiffness in human lenses that was directly correlated with levels of protein–cross-linking AGEs. α-Crystallin in the lens binds to other proteins and prevents their denaturation and aggregation through its chaperone-like activity. Using a FRET-based assay, we examined the stability of the αA-crystallin–γD-crystallin complex for up to 12 days and observed that this complex is stable in PBS and upon incubation with human lens–epithelial cell lysate or lens homogenate. Addition of 2 mm ATP to the lysate or homogenate did not decrease the stability of the complex. We also generated complexes of human αA-crystallin or αB-crystallin with alcohol dehydrogenase or citrate synthase by applying thermal stress. Upon glycation under physiological conditions, the chaperone–client complexes underwent greater extents of cross-linking than did uncomplexed protein mixtures. LC-MS/MS analyses revealed that the levels of cross-linking AGEs were significantly higher in the glycated chaperone–client complexes than in glycated but uncomplexed protein mixtures. Mouse lenses subjected to thermal stress followed by glycation lost resilience more extensively than lenses subjected to thermal stress or glycation alone, and this loss was accompanied by higher protein cross-linking and higher cross-linking AGE levels. These results uncover a protein cross-linking mechanism in the lens and suggest that AGE-mediated cross-linking of α-crystallin–client complexes could contribute to lens aging and presbyopia.

The company, often described as a virtual bulletin board for images, counts the Asia Pacific region as one of its fastest growing ones; it opened an office in Singapore in July to service the Indian and South East Asian markets.

Even deaths did not stop interest in buying potentially dangerous drugs, researchers find

The new Labour Leader has promoted the prominent backbencher Jess Phillips to his top team.

Troy Robinson has been taken on as the new managing director of Chugai Pharma Europe. Mike Crosher will succeed him in his current role as managing director of Chugai Pharma UK.

Chugai Pharma Europe (CPE) has promoted Troy Robinson and Mike Crosher into the positions of managing director of CPE and managing director of the firm’s UK subsidiary, Chugai Pharma UK (CPU).

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Lumos Pharma, Inc. has promoted Dr John McKew to the position of Chief Operating Officer effective 1 April 2020.

McKew is already Chief Scientific Officer and he will do both roles simultaneously. With his new role, he will lead Lumos’s clinical development plan as the company looks toward adding additional assets to its pipeline.

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A federal court in Jackson, Miss., has ruled in favor of the United States involving a businessman’s attempt to use a KPMG- marketed tax shelter to avoid paying income tax on approximately $18 million in capital gains.

A federal court has permanently barred Gerald A. Poynter II of Kansas City, Mo., from preparing federal tax returns for others and from promoting a fraudulent tax scam.

In major relief, the state government has decided that first and second-year students will be promoted to the next year based on performance. However, final year students will have to appear for their final exams which will be conducted between July 1 to 30.

State higher and technical education minister Uday Samant declared the decision through video on Friday providing relief to lakhs of students studying in thousands of colleges affiliated to different universities in the state.

However, the decision to conduct the final year exams will be subject to COVID-19 situation in the state and a decision about the same will be taken on June 20.

Also, a committee has been formed which will decide if the Common Entrance Test (CET) for UG and PG courses can be conducted. The committee is going to table their findings in eight days.

For UG the exam has been scheduled between July 1-19 and July 23-30 for PG courses.

As for students fearing non-compliance of 75 per cent mandatory attendance criteria, Samant said, "All students should be given full attendance for all days of lockdown until now which is 45 days. However even after adding these 45 days if any student is still lagging, the college should consider those cases compassionately. All final year final examination students shall continue studying as their exams will be held in July. It is a question of their career which is why the exams cannot be cancelled."

Explaining the grading system that will be used for first and second-year students, a senior University official said, "This grading will be done with 50:50 formula where 50 per cent marks will be based on internal evaluation or tests held until now before the lockdown and 50 per cent from the performance of the student in the previous year. If previous year's performance is not available, the grading will depend 100 per cent on performance until now in the academic year."

"Moreover students who have pending papers or fail in any subject will have to appear for ATKT exams which the varsities will hold within 120 days from the beginning of the new academic year," he added.

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US regulator cautions against using chloroquine outside of hospitals or clinical trials

Speaking via videolink to the Digital, Culture, Media and Sport Committee on Tuesday, Parry confirmed that discussions have led him to believe that promotion and relegation will go ahead as normal.

Wolverhampton Wanderers have received plenty of deserved plaudits on their return to the top flight, with some going as far as to call them the best newly promoted side in Premier League history.

The EFL are locked in talks about how to resume the campaign behind closed doors amid the coronavirus crisis, which is threatening to bring an early end to the League One and Two seasons.

Speaking via videolink to the Digital, Culture, Media and Sport Committee on Tuesday, Parry confirmed that discussions have led him to believe that promotion and relegation will go ahead as normal.

Swansea City's owners are looking to sell the club for £38 million, but could increase their asking price by £!5m if they're promoted to the Premier League at the end of this season.

Sheffield United's latest win leaves them just two points off a Champions League spot, but how do they compare to other Premier League sides to have taken the top flight by storm following promotion.

Leeds United are looking to bolster their bid for Premier League promotion with a move for Hull City forward Jarrod Bowen. It'd be an initial loan for £3m that could rise to £15m if they win promotion.

Punjab Government has decided to promote students of Class 10 board exams 2020. Candidates who have appeared for the PSEB Class 10 Matric Board exams will be promoted on the basis of their pre-board examination results. Chief Minister of Punjab, Captain

CBSE students studying in classes I-VIII to be promoted to next class

The state government has decided not to conduct exams for 1st and 2nd year and lower semester students due to the COVID-19 pandemic.

In a related-party transaction, Raghav Bahl transfers digital content business from an unlisted firm to a listed one