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IEEE / Institute of Electrical and Electronics Engineers Incorporated

‘It will equip them to express their opinion and they are also supposed to know the allocations made to 39 departments’

This mobile app helps aspirants test their skill, knowledge and writing speed.

Meet decides to appoint Arun S. Nair as Sabarimala ADM to oversee general coordination of the pilgrimage

He requested state governments to cooperate with railways in running of more Shramik special trains.

NETANYA, Israel, May 10, 2020/PRNewswire/ -- Cellcom Israel Ltd. (NYSE: CEL) (TASE: CEL) (the 'Company') announced today that following the Company's previously announced potential debt offering in Israel, the Company is in preparations to raise up to NIS 200,000,000in debentures and options to purchase ordinary shares of the Company, and subject to the completion of the contemplated offering, the Company has accepted early commitments from institutional investors for the purchase of an aggregate of 222,000 units, each comprised of 1,000 debentures Series L and 10 options Series 4, for NIS 906per unit out of orders for approximately 560 thousand units received in the institutional tender. In...

This is a guidance on sample preparation and dosimetry, to which special attention should be paid when testing nanomaterials for human health and environmental safety endpoints.

UFC 249 will be the first major US sporting event since the shutdown due to coronavirus. The main event will see Tony Ferguson fight Justin Gaethje for the interim lightweight championship.

Jessica Ennis-Hill faces a key test of her Olympic preparations in Germany this weekend when she competes in her first heptathlon since winning gold at last year's World Championships.

A simple two-spindle based lathe system for the preparation of cylindrical samples intended for X-ray tomography is presented. The setup can operate at room temperature as well as under cryogenic conditions, allowing the preparation of samples down to 20 and 50 µm in diameter, respectively, within minutes. Case studies are presented involving the preparation of a brittle biomineral brachiopod shell and cryogenically fixed soft brain tissue, and their examination by means of ptychographic X-ray computed tomography reveals the preparation method to be mainly free from causing artefacts. Since this lathe system easily yields near-cylindrical samples ideal for tomography, a usage for a wide variety of otherwise challenging specimens is anticipated, in addition to potential use as a time- and cost-saving tool prior to focused ion-beam milling. Fast sample preparation becomes especially important in relation to shorter measurement times expected in next-generation synchrotron sources.

Structural biology generally provides static snapshots of protein conformations that can provide information on the functional mechanisms of biological systems. Time-resolved structural biology provides a means to visualize, at near-atomic resolution, the dynamic conformational changes that macromolecules undergo as they function. X-ray free-electron-laser technology has provided a powerful tool to study enzyme mechanisms at atomic resolution, typically in the femtosecond to picosecond timeframe. Complementary to this, recent advances in the resolution obtainable by electron microscopy and the broad range of samples that can be studied make it ideally suited to time-resolved approaches in the microsecond to millisecond timeframe to study large loop and domain motions in biomolecules. Here we describe a cryo-EM grid preparation device that permits rapid mixing, voltage-assisted spraying and vitrification of samples. It is shown that the device produces grids of sufficient ice quality to enable data collection from single grids that results in a sub-4 Å reconstruction. Rapid mixing can be achieved by blot-and-spray or mix-and-spray approaches with a delay of ∼10 ms, providing greater temporal resolution than previously reported mix-and-spray approaches.

Single-particle electron cryo-microscopy (cryoEM) has undergone a `resolution revolution' that makes it possible to characterize megadalton (MDa) complexes at atomic resolution without crystals. To fully exploit the new opportunities in molecular microscopy, new procedures for the cloning, expression and purification of macromolecular complexes need to be explored. Macromolecular assemblies are often unstable, and invasive construct design or inadequate purification conditions and sample-preparation methods can result in disassembly or denaturation. The structure of the 2.6 MDa yeast fatty acid synthase (FAS) has been studied by electron microscopy since the 1960s. Here, a new, streamlined protocol for the rapid production of purified yeast FAS for structure determination by high-resolution cryoEM is reported. Together with a companion protocol for preparing cryoEM specimens on a hydrophilized graphene layer, the new protocol yielded a 3.1 Å resolution map of yeast FAS from 15 000 automatically picked particles within a day. The high map quality enabled a complete atomic model of an intact fungal FAS to be built.

Although microscopes and image-analysis software for electron cryomicroscopy (cryo-EM) have improved dramatically in recent years, specimen-preparation methods have lagged behind. Most strategies still rely on blotting microscope grids with paper to produce a thin film of solution suitable for vitrification. This approach loses more than 99.9% of the applied sample and requires several seconds, leading to problematic air–water interface interactions for macromolecules in the resulting thin film of solution and complicating time-resolved studies. Recently developed self-wicking EM grids allow the use of small volumes of sample, with nanowires on the grid bars removing excess solution to produce a thin film within tens of milliseconds from sample application to freezing. Here, a simple cryo-EM specimen-preparation device that uses components from an ultrasonic humidifier to transfer protein solution onto a self-wicking EM grid is presented. The device is controlled by a Raspberry Pi single-board computer and all components are either widely available or can be manufactured by online services, allowing the device to be constructed in laboratories that specialize in cryo-EM rather than instrument design. The simple open-source design permits the straightforward customization of the instrument for specialized experiments.

Serial crystallography, at both synchrotron and X-ray free-electron laser light sources, is becoming increasingly popular. However, the tools in the majority of crystallization laboratories are focused on producing large single crystals by vapour diffusion that fit the cryo-cooled paradigm of modern synchrotron crystallography. This paper presents several case studies and some ideas and strategies on how to perform the conversion from a single crystal grown by vapour diffusion to the many thousands of micro-crystals required for modern serial crystallography grown by batch crystallization. These case studies aim to show (i) how vapour diffusion conditions can be converted into batch by optimizing the length of time crystals take to appear; (ii) how an understanding of the crystallization phase diagram can act as a guide when designing batch crystallization protocols; and (iii) an accessible methodology when attempting to scale batch conditions to larger volumes. These methods are needed to minimize the sample preparation gap between standard rotation crystallography and dedicated serial laboratories, ultimately making serial crystallography more accessible to all crystallographers.

Engineering is emerging as an important topic in K-12 education in the U.S., and is being incorporated into education standards, instructional materials, and assessments.

SUFFOLK – The Virginia Department of Transportation Hampton Roads District (VDOT) is suspending lane closures on Interstate 64 and Interstate 664...

SUFFOLK– As Hurricane Florence approaches the coast, the Virginia Department of Transportation (VDOT) Hampton Roads District continues to monitor...

Whether you're planning to camp or just go for a simple hike, here are 10 things you need before you hit the trail.

Tax return preparation can be done in several ways to calculate your taxes and pay it to the government in time. You just have to make choice of finding out the best way of doing this to pay your taxes.

Diplomacy: 17 Nations from around the world are officially supporting the privately organised expedition ANTARCTIC BLANC. Challenging in ten days under sails twice the Drake Passage to Antarctica to establish a peaceful international dialogue.