Joaquin Phoenix, who discussed animal cruelty in his acceptance speech, was one of several famous vegans at the Oscars, including Billie Eilish.

Hear about travel to the Silk Road in China's Gansu Province as the Amateur Traveler talks again to Lee Moore from Silk Road Hitchhikers about his trip to this historic and remote part of China.

Fabry disease is a heritable lipid disorder caused by the low activity of α-galactosidase A and characterized by the systemic accumulation of globotriaosylceramide (Gb3). Recent studies have reported a structural heterogeneity of Gb3 in Fabry disease, including Gb3 isoforms with different fatty acids and Gb3 analogs with modifications on the sphingosine moiety. However, Gb3 assays are often performed only on the selected Gb3 isoforms. To precisely determine the total Gb3 concentration, here we established two methods for determining both Gb3 isoforms and analogs. One was the deacylation method, involving Gb3 treatment with sphingolipid ceramide N-deacylase, followed by an assay of the deacylated products, globotriaosylsphingosine (lyso-Gb3) and its analogs, by ultra-performance LC coupled to tandem MS (UPLC-MS/MS). The other method was a direct assay established in the present study for 37 Gb3 isoforms and analogs/isoforms by UPLC-MS/MS. Gb3s from the organs of symptomatic animals of a Fabry disease mouse model were mainly Gb3 isoforms and two Gb3 analogs, such as Gb3(+18) containing the lyso-Gb3(+18) moiety and Gb3(−2) containing the lyso-Gb3(−2) moiety. The total concentrations and Gb3 analog distributions determined by the two methods were comparable. Gb3(+18) levels were high in the kidneys (24% of total Gb3) and the liver (13%), and we observed Gb3(−2) in the heart (10%) and the kidneys (5%). These results indicate organ-specific expression of Gb3 analogs, insights that may lead to a deeper understanding of the pathophysiology of Fabry disease.

Fabry disease is a heritable lipid disorder caused by the low activity of α-galactosidase A and characterized by the systemic accumulation of globotriaosylceramide (Gb3). Recent studies have reported a structural heterogeneity of Gb3 in Fabry disease, including Gb3 isoforms with different fatty acids and Gb3 analogs with modifications on the sphingosine moiety. However, Gb3 assays are often performed only on the selected Gb3 isoforms. To precisely determine the total Gb3 concentration, here we established two methods for determining both Gb3 isoforms and analogs. One was the deacylation method, involving Gb3 treatment with sphingolipid ceramide N-deacylase, followed by an assay of the deacylated products, globotriaosylsphingosine (lyso-Gb3) and its analogs, by ultra-performance LC coupled to tandem MS (UPLC-MS/MS). The other method was a direct assay established in the present study for 37 Gb3 isoforms and analogs/isoforms by UPLC-MS/MS. Gb3s from the organs of symptomatic animals of a Fabry disease mouse model were mainly Gb3 isoforms and two Gb3 analogs, such as Gb3(+18) containing the lyso-Gb3(+18) moiety and Gb3(−2) containing the lyso-Gb3(−2) moiety. The total concentrations and Gb3 analog distributions determined by the two methods were comparable. Gb3(+18) levels were high in the kidneys (24% of total Gb3) and the liver (13%), and we observed Gb3(−2) in the heart (10%) and the kidneys (5%). These results indicate organ-specific expression of Gb3 analogs, insights that may lead to a deeper understanding of the pathophysiology of Fabry disease.

London : Rebman, 1901.

New York ; London : G.P. Putnam, 1891.

Berlin : Peter Lang [2019]

M Chalfie
Apr 1, 1985; 5:956-964
Articles

The project aimed to improve the transport conditions and services in Longnan municipality in the People's Republic of China. The targeted outcomes were the provision of efficient, safe and reliable road transport in the project area; and improved access to markets, income generating opportunities, education and health services and social activities for rural people. Key to this was a targeted reduction in travel times of between 5 and 7 hours, with 90% of the villages being served by an all-weather road.

Gene-edited pigs and brain implants are blurring the lines of what it means to be human, so our morals and laws may need to change to include beings that are “substantially human”

Key Points

Kathryn Knight

McCoy is Keough's second child with her husband, Kyle Bosworth

Looking for amazing Thanksgiving recipes that cater to those of us skipping meat? We've got you covered! Check out these fantastic ideas for appetizers, sides, mains and dessert!

Vegans will love this cookbook that has grown out of a popular Indian food blog. Now it's possible to enjoy classics like tofu-paneer in spinach and chicken-free balti.

This is fashion with compassion -- beautiful footwear that causes no harm to animals and minimal damage to the planet.

Unfortunately, people like the slogans a bit too much.

The Sena chief says if BJP opts out in Palghar, he will support the ally in Gondia-Bhandara, which too will go to Lok Sabha bypoll on the same day (May 28).

Shiv Sena may have discovered a way to bend the BJP its way. The political precision with which the party snapped up the disgruntled son of a late BJP parliamentarian for fielding in the Palghar Lok Sabha bypoll has taken Uddhav's resolve of outsmarting the saffron frenemy to a decisive level. Uddhav wants to fly solo, escape BJP's trap for emerging as the single largest party in the state next year.

Palghar, a testing lab, may decide the fate of the strained relation between the two partners, which the Sena is using to try and carve a niche for itself by playing a detractor within and outside the government.

Uddhav has a spring in his step, as Sena prepares to run Palghar, and the guts to ask the BJP to withdraw from the race. It would be interesting to see who blinks first in a barter proposed by Uddhav. The Sena chief says if BJP opts out in Palghar, he will support the ally in Gondia-Bhandara, which too will go to Lok Sabha bypoll on the same day (May 28).

But will they reach a compromise? The possibility of the two parties backing out seem remote. There are others in the fray who too want the BJP defeated. The BJP has fielded a former Congress junior minister, Rajendra Gavit, who had started his political career with Janata Dal. Gavit came in amid high drama after Sena's nomination of BJP's late MP Chintaman Wanga's son Shrinivas surprised all. Gavit had lost two Assembly elections in a row for the Congress, and before that, he was unceremoniously asked to pull out for a BVS candidate in 2014 Lok Sabha polls.

'Well played, Sena'
The BJP camp does not admit it openly, but insiders say the Sena was tactically much superior this time. Sena explored the emotional turmoil in the Wanga family, which felt ignored by the BJP. The family members accused the BJP high command, including the CM, of not giving them audience. In response, Fadnavis blamed the poaching on Sena. He said it was unexpected of the ally, and that the BJP would win, with or without Sena.

The Palghar bypoll is important for the BJP to reaffirm its influence in the tribal belt. In the 2014 Lok Sabha elections, four out of the 48 seats were reserved for Scheduled Tribes, of which all went to the BJP, an unprecedented result. BJP had been contesting Dahanu (delimited and named Palghar) for many decades but with limited success.

In the Assembly, 25 of the 288 seats are reserved for STs. Of these, BJP won 11, followed by Congress's five, NCP's four, Sena's three and one each by CPI(M) and Bahujan Vikas Aghadi (BVA).

The results of Lok Sabha bypolls in Maharashtra should measure BJP's influence in the four years of government. Sena hasn't fielded in Gondia-Bhandara because its poor strength will never secure it a prominent place. Knowing his weakness in the region well, Uddhav paid an impromptu visit to Nagpur last week. Again, his was an attempt to create some nuisance for the BJP, where they expect BJP and NCP to fight straight.

Other factors in Palghar
For Sena, Palghar has turned out to be a sharper tool to cut the BJP to size. Uddhav has nothing lose in a skirmish that will be four-way in case some candidates don't pull out, while a victory would be a shot in the arm for Sena.

Former allies Congress and influential local outfit, led by Hitendra Thakur, BVA are also in the fray. BVA's role will be significant in splitting votes because it had won the LS seat in 2009, and lost the next to BJP, despite having its vote share of 30 per cent intact. In 2014, BJP increased its vote share by a whopping 25 per cent. BJP and Sena were together then, while Congress had withdrawn Gavit (now BJP's candidate) for supporting Thakur's candidate. BVA continues to be a big player in two urban Assembly segments of Vasai and Nalasopara, which Thakur and son represent.

If it were to ensure BJP's defeat, will Congress withdraw the candidature of former MP Damu Shingda this time? Congress insiders say supporting BVA may not be a good idea, and instead, the party should have a tacit understanding with the Sena for beating the BJP at the hands of the ally. But will that be acceptable to Congress, which is working to emerge as a strong alternative to BJP?

All eyes will be on developments taking place on Monday, the last day of withdrawal of candidatures.

Dharmendra Jore is political editor, mid-day. He tweets @dharmendrajore Send your feedback to mailbag@mid-day.com

Catch up on all the latest Mumbai news, crime news, current affairs, and also a complete guide on Mumbai from food to things to do and events across the city here. Also download the new mid-day Android and iOS apps to get latest updates

Nine patients infected with hepatitis C virus (HCV) after receiving lifesaving heart transplants from deceased donors who were infected with the disease

Doctors can safely transplant hepatitis C-infected hearts and lungs into people who are in dire need for a new organ, reports a new study. The findings

A tool to assess organ acceptance practices by transplant centers found wide variability in centers' willingness to use less-than-ideal donor kidneys has been developed by researchers.

Screenwriter and producer Alex Gansa has shed light on the source of some of the eerie similarities between the tragic real-life events of recent months, specifically the Paris attacks.

The manifesto encourages members to wage a war on Australian farmers by entering their properties, 'documenting the conditions' and stealing animals.

The unusual appearance of the treehopper insect from Nicaragua - Kaikaia gaga - brings to mind the outlandish costumes worn by the pop star Lady Gaga.

Lily Burns, 21, from Fort Williams, tno health conditions when she caught coronavirus, and told BBC's Good Morning Scotland how it attacked her organs and left her fighting for her life.

'During a visit to One25, a charity which helps female sex workers in Bristol, Meghan did something very strange indeed. On each banana, she carefully inked a self-help message,' writes Jones.

One £15.45 shirt on eBay said: 'I survived the Coronavirus and all I got was this lousy T-shirt.' Others on the auction site mocked the city in China at the epicentre of the outbreak.

Adult human stem cells have been sent to the International Space Station (ISS) this week to explore how low gravity affects the growth of human tissue.

There's much to ponder over at the bookshelf this weekend.

Cham : Springer, 2020.

Online Resource

Proulx, Vincent-Joël, author

Online Resource

Hayden Library - BF175.Z83 2019

Hayden Library - R857.T55 D43 2017

Online Resource

Online Resource

Online news editor David Grimm shares stories on evidence for the earth being hit by supernovae, record-breaking xenotransplantation, and winning friends and influencing people with human sacrifice.   Staff news writer Jocelyn Kaiser joins host Sarah Crespi to discuss how small membrane-bound packets called “exosomes” might pave the way for cancer cells to move into new territory in the body.     [Image: Val Altounian/Science]    

Dhumal admitted that quarantining of Indian players upon their arrival is a possibility, if their tour Down Under goess as per schedule.

Interview with Nationalist Congress Party member of Parliament

Rotch Library - DS99.D8 E34 2011

Online Resource