The final 3D atlas of the sky will help scientists study dark matter and dark energy, which make up 96 percent of the universe but remain mysterious

The sprawling estate, which is the largest privately owned home in the country, will open its doors in November after a month-long closure

Creating transgenic mosquitoes allows for mechanistic studies of basic mosquito biology and the development of novel vector control strategies. CRISPR–Cas9 gene editing has revolutionized gene editing, including in mosquitoes. This protocol details part of the gene editing process of Aedes aegypti mosquitoes via CRISPR–Cas9, through testing and validating single-guide RNAs (sgRNAs). Gene editing activity varies depending on the sequence of sgRNAs used, so validation of sgRNA activity should be done before large-scale generation of mutants or transgenics. sgRNA is designed using online tools and synthesized in <1 h. Once mutants or transgenics are generated via embryo microinjection, sgRNA activity is validated by quick genotyping polymerase chain reaction (PCR) and DNA sequencing.

Transposon-mediated transgenesis has revolutionized both basic and applied studies of mosquito vectors of disease. Currently, techniques such as enhancer traps and transposon tagging, which rely on remobilizable insertional mutagenesis, are only possible with transposon-based vector systems. Here, we provide general descriptions of methods and applications of transposon-based mosquito transgenesis. The exact procedures must be adapted to each mosquito species and comparisons of some differences among different mosquito species are outlined. A number of excellent publications showing detailed and specific protocols and methods are featured and referenced.

Paraglider Ben Lewis remembers spinning around wildly as he got pulled up higher and higher into the violent storm. That was when he realized, about 6,700 metres up in the sky and still rising, that he was not likely to see his family again.

After Canada Post workers gave a 72-hour notice to strike early Tuesday, some Calgary business owners are worried about what this means for holiday season sales. 

CRISPR–Cas9 has revolutionized gene editing for traditional and nontraditional model organisms alike. This tool has opened the door to new mechanistic studies of basic mosquito biology as well as the development of novel vector control strategies based on CRISPR–Cas9, including gene drives that spread genetic elements in the population. Although the promise of the specificity, flexibility, and ease of deployment CRISPR is real, its implementation still requires empirical optimization for each new species of interest, as well as to each genomic target within a given species. Here, we provide an overview of designing and testing single-guide RNAs for the use of CRISPR-based gene editing tools.

Pioneering Technology Allows Surgeons to Cut Precisely and Control Bleeding Without Causing Heat Damage to Tissue

Centuries-old university standardizes on SolidWorks Education Edition software so students can learn CAD and design validation skills

Knee replacement doesn’t have to be ‘total’

Flagship Data Management Solution Introduces‘Item-Centric’ PDM and New BOM Functionality

Quick design turnaround and easy documentation process help UK-based company move products to market faster

Robotics programmers can now use SolidWorks 3D CAD models for more powerful simulations

R&D ‘Sandbox’ Previews Some Potential Capabilities for 3D CAD Software

Switch to SolidWorks 3D CAD and Simulation Software Delivers Productivity Increase over AutoCAD

Sir Richard Branson, Jeff Ray, Jon Hirschtick, and World-Class Companies to Shine at World’s Largest Annual SolidWorks Gathering

2008 Class 1 World Powerboat Champions Design Faster Boats Using SolidWorks 3D CAD and Simulation Software

Austrian manufacturer also optimizes creation of assembly documentation and related bills of material

Eliminates the Cost and Complexity of Maintaining Online Parts Catalogs; Improves Search, Navigation, and Communication for Engineers Seeking 3D Part Models

SolidWorks World 2009 Wraps Up Exhilarating Week of Learning and Networking

Control Engineering Recognizes SolidWorks® 2009 for Design Simulation

Free SolidWorks 3D CAD Software, Training, and Certification will Build Valuable New Skills Employers Need

Business-Critical Solutions are Now Free to any Subscriber for an Additional Year

Solutions Catch Problems Early and Drive Cost Out of Designs

New Licensing, Support Programs For Certification Accompany Software Upgrades

Leading elevator components supplier implements 550 licenses of the SolidWorks PDM solution

One Of Only Four Companies To Place Among Top Employers In Each Year Of The Publication’s Survey

Maker of NHL Hockey Boards Uses SolidWorks and Robotics to Speed Production by More than 60 Percent

Displaced Workers Get Free SolidWorks CAD Software,Learn It, and Obtain New Jobs

Integrated Tools Make Mechatronics-Oriented Design and Seamless Deployment to Hardware Easy

Leading CAD Software Optimizes Core Design Functions for an Improved Design Experience

3D CAD Software’s Superior Data Translation Capabilities Support Growth of Tech Publishing and Engineering Services

SolidWorks’ ‘Balance of Ease of Use and Powerful Capabilities’Prompts Rapid Expansion of Student Access

Non-Profit Cooperative Assembled Virtual Team to Prove $1,000 Incubator Was Possible, Safe, and Reliable

SolidWorks Drives Custom 3D CAD Models, Helping the Company Outperform Competition

Engineers Use 3D CAD and Simulation To Customize Products With Quick Modifications, Testing, and Demonstrations

367.382 mph in a Two-Wheeled Missile

Curventa and RuRoc Adapt Original Design for Formula One Pit Crew Safety Wear

CAD and Documentation Solutions Enable U.K.-Based Company To Create Bath Fixtures For Safe, Easy Access

Sharing Information Between Design and Production Eliminates Budget Overruns That Erode Customers’ Profits

Zero-Gravity Arm Reduces Injuries, Saves Money, and Improves Product Quality

World Cup Champion Noelle Pikus-Pace Turns To Local Metal Shop – And Her Husband – For Innovative New Sled

Intuitive 3D CAD Software, Used So Often by Professionals, Prepares EUSS Students for Success

New Solution Helps Designers Calculate and Reduce the Environmental Impact of Their Materials, Production Methods

‘Let’s Go Design’ TV Debuts Today

Ramac Analyzes Design With SolidWorks SustainabilityXpress, Finds Alternatives That Consume Fewer Resources

Intuitive Software Fosters Rapid Engagement with Engineering Challenges

Easy-to-Learn CAD Software Keeps Ideas Flowing