hari Charity Scam - From Mrs Sulak By feedproxy.google.com Published On :: Mon, 21 Apr 2014 22:58:33 +0200 Mrs Sulak is dying, but she will always stay alive, long enough for someone to reply. That's 419 scam law! Full Article
hari Charity Scam - YOUR DONATION FUND(REPLY) By feedproxy.google.com Published On :: Tue, 09 Sep 2014 22:56:51 +0200 Margaret Loughrey wants to give you some of her winnings... not really... 419 scammers are piggybacking on her story, which is already OLD news. Full Article
hari East Rutherford Metal Band Harvest Falls Debuts New Single And Charity Drive By feedproxy.google.com Published On :: NJ Rock Band Harvest Falls Debuts Video, Single, Charity Drive For New Song "MOMENT OF CONSEQUENCE" Full Article
hari Eddie Murphy Headlines Comedy Fest For Charity By feeds.bet.com Published On :: Fri, 1 May 2020 18:03:00 EDT “Laughter is often the best medicine.” Full Article Celebrities
hari Calboy - "Chariot" By feeds.bet.com Published On :: Sun, 23 Jun 2019 17:57:33 EDT Calboy performs "Chariot." Full Article BET Experience
hari Calboy - "Chariot" By feeds.bet.com Published On :: Sun, 23 Jun 2019 17:57:33 EDT Calboy performs "Chariot." Full Article BET Experience
hari Michael Jordan Donates ‘The Last Dance’ Proceeds To Charity By feeds.bet.com Published On :: Tue, 21 Apr 2020 12:00:00 EDT The doc made history for ESPN. Full Article Michael Jordan Sports News
hari ‘Covid Candles’ Fundraiser Assists Charities By bernews.com Published On :: Mon, 04 May 2020 11:50:39 +0000 Owen Stewart, who makes candles as a hobby, has taken the initiative to support the community during the Covid-19 pandemic by selling ‘Covid Candles,’ with the proceeds going to local charities. Amanda Stewart, Owen’s mother, said, “This started for him as a rainy day hobby for he and I to do together. We would save […](Click to read the full article) Full Article All Entertainment News #CharityEvents #Covid19 #GoodNews
hari Airport Charity Classic Raises Over $30,000 By bernews.com Published On :: Tue, 03 Dec 2019 16:50:52 +0000 The Airport Charity Classic organized by Skyport, Aecon & Bermuda Airport Authority raised more than $30,000 for two local charities in its second year. The event was held on October 10 at Tucker’s Point Golf Club with all proceeds going to Tomorrow’s Voices and SCARS [Saving Children and Revealing Secrets]. Twenty-eight teams registered to play […](Click to read the full article) Full Article All News Sports #Aecon #BermudaGolf #CharityEvents #GoodNews
hari Video: ‘Taking Off’ Highlights Charity Golf Classic By bernews.com Published On :: Thu, 05 Dec 2019 14:10:09 +0000 Skyport has released the latest episode of ‘Taking Off’, a video series with host Mikaela Pearman that highlights the airport redevelopment project, with this episode highlighting the Airport Charity Golf Classic. Speaking in the episode, Mikaela Pearman said, “Skyport, Aecon and the Bermuda Airport Authority held the second annual Airport Charity Golf Classic in October. […](Click to read the full article) Full Article All Business Sports Videos #Airport #AirportRedevelopment #BermudaBusiness #BermudaGolf #CharityEvents #SkyportTakingOff
hari Photos & Video: Classic Bike Club Charity Ride By bernews.com Published On :: Mon, 29 Feb 2016 13:58:45 +0000 The Bermuda Classic Bike Club hosted their annual charity bike ride on Sunday, February 28th, with proceeds going to the Coalition for the Protection of Children. Dozens of bikes gathered in front of City Hall and the event got underway at 10.30am with the group leaving Hamilton and travelling to St. George’s Town Square via […](Click to read the full article) Full Article All Cars/Bikes News Photos Videos #ClassicBikeClub #GoodNews
hari Fifty-Eight Rowers Raise $3,000 For Charity By bernews.com Published On :: Wed, 03 Jan 2018 12:16:12 +0000 Fifty-eight rowers raised more than $3,000 for a local charity last month by rowing almost 400 miles. The rowers from LifeThyme CrossFit, CrossFit BDA, Beyond Fit, the Bermuda Rowing Association, and a team of Warwick Academy students each tackled 40 kilometers in teams of four and two on rowing machines to raise a total of […](Click to read the full article) Full Article All News Sports #CharityEvents #GoodNews #Rowing
hari Charity Screenings Of Downton Abbey Movie By bernews.com Published On :: Sun, 15 Sep 2019 15:00:42 +0000 Downton Abbey is revisited in a newly released movie continuation of the hugely popular TV historical soap opera. Bermudian fans of the show can catch up with the post-World War One goings-ons of the aristocratic Crawley family and their domestic servants as well as help a good cause at two charity screenings of the film […](Click to read the full article) Full Article All Entertainment Films/Movies #CharityEvents #FullLengthMovies
hari P.A.L.S. To Benefit From IWC Charity Art Exhibit By bernews.com Published On :: Tue, 04 Feb 2020 11:23:41 +0000 The annual Charity Art Exhibition sponsored by The International Women Club of Bermuda is scheduled to take place on Friday, March 6th, at The Gallery in the Chubb Building. A spokesperson said, “The annual Charity Art Exhibition sponsored by The International Women Club of Bermuda is scheduled to take place at The Gallery in the […](Click to read the full article) Full Article All Entertainment #Artists #CharityEvents #InternationalWomensClub
hari Bermudian Singer In UK Music Charity Venture By bernews.com Published On :: Fri, 18 Oct 2019 11:00:18 +0000 Bermudian entertainer Janita Adderley is part of a charity initiative in the UK which is using music to raise awareness and funds for those suffering from mental health issues. Singer Ms Adderley, a member of the Bermuda group The Loquats and the all-female band Stiletto 5, studied at York College and still spends time in […](Click to read the full article) Full Article All Entertainment Music News #BermudianSingers #CharityEvents #Health #Music
hari A Night of Sharing Culture By fisher.osu.edu Published On :: Fri, 14 Feb 2020 23:07:35 +0000 Over the weekend I attended one of my fellow classmates, Karina Ribeiro Teixeira, birthday celebration. Karina is a first-year international student from Brazil in the MHRM program and we also work together in the Graduate Program Office together. She and her husband were gracious enough to host us at their home and share some traditional … Continue reading "A Night of Sharing Culture" Full Article Exploring Columbus Fisher College of Business MHRM Social Events
hari Digging up Positivity - Furry charity and good news - February 2020 (Transcript) By www.flayrah.com Published On :: Mon, 02 Mar 2020 23:26:42 +0000 Video from Thabo Meerkat, transcribed Welcome to another edition of Digging Up Positivity! This episode is dedicated to the many volunteers that make all those amazing conventions and charities possible. But besides them, we are covering some animation news and other (maybe otter?) tidbits! read more Full Article animal behaviour animation charity conventions fursuits Sonic the Hedgehog YouTube
hari Digging up Positivity - Furry charity and good news - April 2020 By www.flayrah.com Published On :: Sat, 25 Apr 2020 16:32:51 +0000 Video from Thabo Meerkat, transcribed Hey there, and welcome to the April 2020 edition of Digging Up Positivity from a rapidly changing world. But even in these weird times, there are still a lot of positive things to be found! read more Full Article animal behaviour charity conventions interviews transcription YouTube
hari charity work by NaruKoibito [G] By www.siye.co.uk Published On :: Mon, 13 Apr 2020 17:47:58 -0400 He'd only meant it as a joke, but here she was. Ginny Weasley, his celebrity crush, armed with economy toilet paper rolls and three dozen eggs, ready to commit a misdemeanor all in the name of charity. Muggle AU Full Article
hari The sound and sense of Tocharian By languagelog.ldc.upenn.edu Published On :: Mon, 04 May 2020 04:07:38 +0000 Readers of Language Log will certainly be aware of Tocharian, but when I began my international research project on the Tarim Basin mummies in 1991, very few people — only a tiny handful of esoteric researchers — had ever heard of the Tocharians and their language since they went extinct more than a millennium ago, […] Full Article Borrowing Decipherment Historical linguistics
hari Open COVID Pledge: Removing Obstacles to Sharing IP in the Fight Against COVID-19 By creativecommons.org Published On :: Tue, 07 Apr 2020 21:42:20 +0000 Creative Commons has joined forces with other legal experts and leading scientists to offer a simple way for universities, companies, and other holders of intellectual property rights to support the development of medicines, test kits, vaccines, and other scientific discoveries related to COVID-19 for the duration of the pandemic. The Open COVID Pledge grants the … Read More "Open COVID Pledge: Removing Obstacles to Sharing IP in the Fight Against COVID-19" The post Open COVID Pledge: Removing Obstacles to Sharing IP in the Fight Against COVID-19 appeared first on Creative Commons. Full Article About CC covid-19 open covid pledge open patents
hari Why Sharing Academic Publications Under “No Derivatives” Licenses is Misguided By creativecommons.org Published On :: Tue, 21 Apr 2020 13:17:48 +0000 The benefits of open access (OA) are undeniable and increasingly evident across all academic disciplines and scientific research: making academic publications1 freely and openly accessible and reusable provides broad visibility for authors, a better return on investment for funders, and greater access to knowledge for other researchers and the general public. And yet, despite OA’s obvious … Read More "Why Sharing Academic Publications Under “No Derivatives” Licenses is Misguided" The post Why Sharing Academic Publications Under “No Derivatives” Licenses is Misguided appeared first on Creative Commons. Full Article Education / OER Legal tools / licenses CC BY-NC-ND CC BY-ND OER open educational resources
hari Understanding, celebrating, sharing. Be part of it! By feeds.iriss.org.uk Published On :: Fri, 09 Sep 2016 09:34:56 +0000 Iriss is working to raise the profile of the social services workforce by sharing practitioner experiences of working in care and support. We know that often, research about the social services workforce fails to reach those who work most closely with those supported by services, so we need your help to make sure that your voice is heard. read more Full Article
hari Sharing practice to improve outcomes for care leavers. Evaluation report on an inter-authority learning exchange By feeds.iriss.org.uk Published On :: Monday, November 30, 2015 - 09:44 Evaluation report for the inter-authority learning exchange between Shetland Islands, Falkirk and Glasgow Councils throughcare and aftercare teams. In February 2014 a member of the Throughcare and Aftercare team from Shetland, spent two weeks in each host authority as a means of developing and sharing practice, experience and learning. The report describes the planning process, in-situ experience, and post-exchange learning of participants, it also report highlights the positive learning outcomes and benefits achieved for all participating local authorities. The report identifies ideas for future applications of such a learning and practice exchange model to improve practice for looked after young people and care leavers. Full Article
hari Recent information-sharing and translation efforts show that the Middle East and North Africa are top of mind for NFPA By feedproxy.google.com Published On :: 2020-04-14T21:19:31Z During this difficult time, as the world witnesses the relentless spread of COVID-19, the National Fire Protection Association (NFPA) has released several new resources to help stakeholders with life safety efforts. Two documents, in particular, an Full Article fire service codes and standards ppe international emergency preparedness nfpa 1600 united arab emirates global contamination mena middle east nfpa 1999 nfpa 1581 cdc covid-19 coronavirus saudi patient safety center world health organization infection control
hari Book Week 2019: David Shariatmadari's Don't Believe a Word By separatedbyacommonlanguage.blogspot.com Published On :: Fri, 29 Nov 2019 00:05:00 +0000 Welcome to the third review post of Book Week 2019. In the intro to Book Week 2019, I explain what I'm doing this week. In the end, there will be four posts. I thought there would be five, but one of the books has (orig. BrE) gone missing. Having had a day off yesterday, I will also have a day off tomorrow, so the final review will appear during the weekend. Probably.Anyhow, today's book is: Don't believe a wordthe surprising truth about languageby David ShariatmadariNorton, 2019 (N America)W&N, 2019 (UK/RoW)David Shariatmadari writes for the Guardian, often about language, and is one of the sensible journalists on the topic. The number of sensible journalists writing about language has really shot up in the past decade, and judging from reading their books, this is in part because of increasingly clear, public-facing work by academic linguists. (Yay, academic linguists!) But in Shariatmadari's case, the journalist is a linguist: he has a BA and MA in the subject. And it shows—in the best possible way. The book is a familiar genre: busting widely held language myths. If you've read books in this genre before, you probably don't need these myths busted. You probably know that linguistic change is natural, that the border between language and dialect is unfindable, that apes haven't really learned sign languages, and that no form of language is inherently superior to another. Nevertheless, you may learn something new, since Shariatmadari's tastes for linguistic research and theories is not always on the same wavelength as some other books directed at such a general audience. Once again, I'm reviewing with a partial view of the book (this is the practical law of Book Week 2019). In this case, I've read chapters 1, 5, and 9 and skimmed through other bits. The introductory chapter gives us a bit of insight into Shariatmadari's conversion to full-blown linguist, as a reluctant student of Arabic who was quickly converted to admiration for the language and to the study of language as an insight into humanity. "It's not hyperbole to say that linguistics is the universal social science", he writes. "It intrudes into almost every area of knowledge."UK coverI chose to read chapter 5 because I'd had the pleasure of hearing him talk about its topic at a student conference recently: the popularity of "untranslatable word" lists. Goodness knows, I've contributed to them. What I liked about the talk was his detective work on the words themselves—some of the words and definitions presented in lists of 'untranslatables' are practically fictional. And yet, those of us who don't speak the language in question often eat up these lists because of our ethnocentric need to exotici{s/z}e others. This leads inevitably to discussion of linguistic relativism—the notion that the language you speak affects the way you think—and the bad, old (so-called) evidence for it and the newer evidence for something much subtler. The chapter then goes in a direction I wasn't expecting: introducing Natural Semantic Metalanguage (NSM), an interesting (but far from universally taught) approach to meaning that uses about 65 semantic building blocks to represent and compare meanings across languages. NSM adherents make the case that few, if any, words are truly equivalent across languages. But while any word in one language may have no single-word equivalent in another language, that doesn't mean those words are untranslatable. It just means that translating them can be a delicate and complicated thing. US coverThe final chapter (9) takes the opposite view to David Adger's Language Unlimited (in my last review), and argues that the hierarchical (and human-specific) nature of linguistic structure need not be the product of an innate Universal Grammar, but instead could arise from the complexity of the system involved and humans' advanced social cognition. While Adger had a whole book for his argument, Shariatmadari has 30-odd pages, and so it's not really fair to compare them in terms of the depth of their argumentation, but still worth reading the latter to get a sense of how linguists and psychologists are arguing about these things.Shariatmadari is a clear and engaging writer, and includes a good range of references and a glossary of linguistic terminology. If you know someone who still believes some language myths, this might be a good present for them. (Though in my experience, people don't actually like getting presents that threaten their worldview. I still do it, because I care more about myth-busting writers earning royalties than I care about linguistic chauvinists getting presents they want.) It would also make an excellent gift for A-level English and language students (and teachers) and others who might be future linguists. After they read it, send them my way. I love having myth-busted students. Full Article books grammar linguistic relativity
hari Robert auctioning Daydream guitar for charity By craigjparker.blogspot.com Published On :: Sat, 31 Aug 2019 18:55:00 +0000 https://t.co/BoAi0VA5gRRobert Smith of The Cure's 'Schecter Signature UltraCure' Guitar, Played at Pasadena Daydream Festival on August 31stYour bid supports: Free Arts For Abused Children, Mr. Holland's Opus Foundation, Little Kids Rock, Inner-City Arts, The Painted Turtle pic.twitter.com/skBiHZslmy— The Cure (@thecure) August 29, 2019 Robert Smith is donating the guitar he will be playing this weekend at Pasadena Daydream Festival to be auctioned off to benefit @FreeArtsLA, @InnerCityArts, @littlekidsrock, @HollandsOpusFdn and @PaintedTurtleCA! ????bid here????https://t.co/eZ9QdO3lgt— goldenvoice (@goldenvoice) August 27, 2019 Full Article
hari Is the Supply of Charitable Donations Fixed? Evidence from Deadly Tornadoes -- by Tatyana Deryugina, Benjamin M. Marx By www.nber.org Published On :: Do new societal needs increase charitable giving or simply reallocate a fixed supply of donations? We study this question using IRS datasets and the natural experiment of deadly tornadoes. Among ZIP Codes located more than 20 miles away from a tornado's path, donations by households increase by over $1 million per tornado fatality. We find no negative effects on charities located in these ZIP Codes, with a bootstrapped confidence interval that rejects substitution rates above 16 percent. The results imply that giving to one cause need not come at the expense of another. Full Article
hari Editorial: Closing houses of worship during the pandemic is an act of faith and charity By www.latimes.com Published On :: Thu, 2 Apr 2020 06:00:07 -0400 Communing online is a necessity during the COVID-19 pandemic. Full Article
hari Readers React: Article examines Sterling charity ads, but reader questions remain By www.latimes.com Published On :: Mon, 5 May 2014 20:31:00 -0400 Print readers of the Los Angeles Times are no doubt familiar with Donald Sterling's advertisements. Full Article
hari Jewish celebrity Saturday night Passover seder yields $2.5M for charity By www.jpost.com Published On :: Wed, 15 Apr 2020 13:49:20 GMT The Saturday Night Passover Seder that aired on YouTube over the weekend brought together dozens of celebrities and raised $2.6 million for the CDC Foundation. Full Article Passover Charity Seder CDC
hari Dude rented me a Tesla on Turo. It's clear how its car-sharing model is different By www.latimes.com Published On :: Thu, 26 Dec 2019 09:00:22 -0500 Peer-to-peer car sharing seems strange but turns out to be easy, if a bit expensive. Full Article
hari Talia Shire on 'Rocky,' 'Godfather' and Brando's 'great acting charisma' By www.latimes.com Published On :: Mon, 4 May 2020 10:00:22 -0400 Talia Shire has a new release, 'Working Man,' that she calls a powerful film that 'spoke to my soul.' Full Article
hari Virtual Grand National raises £2.6m for NHS Charities Together to aid coronavirus battle By feedproxy.google.com Published On :: Mon, 06 Apr 2020 00:01:00 +0100 Profits of £2.6million have been donated to NHS Charities Together after Saturday's Virtual Grand National. Full Article
hari Justin Rose gives NHS Charities Together Cup boost as golf star eyes rescheduled Ryder Cup By feedproxy.google.com Published On :: Sat, 25 Apr 2020 00:00:00 +0100 EXCLUSIVE: Justin Rose opens up on applauding the NHS from afar, supporting online fundraising efforts with fellow celebrities and why the Ryder Cup should not take place behind closed doors. Full Article
hari The Hobbit live reading: How to watch Andy Serkis 12-hour charity reading of The Hobbit By feedproxy.google.com Published On :: Thu, 07 May 2020 15:10:00 +0100 THE HOBBIT is exactly the story we need to escape from normal life - and now Andy Serkis will take on a mammoth reading. Full Article
hari Charity boost as £16million fund launched over coronavirus crisis By www.express.co.uk Published On :: Sat, 09 May 2020 06:51:00 +0100 CHARITIES working to deliver food to the homeless and others in urgent need of support during the coronavirus emergency were last night promised a £16million boost from the Government. Full Article
hari Varvel: How a new Indiana charity helps families of sick kids By rssfeeds.indystar.com Published On :: Fri, 30 Nov 2018 12:00:08 +0000 Bringing hope, happiness and healing to families raising kids with life-threatening illnesses Full Article
hari Peyton Manning roasts Tom Brady in announcing charity golf event By rssfeeds.indystar.com Published On :: Thu, 07 May 2020 19:07:03 +0000 Peyton Manning, Tom Brady, Tiger Woods and Phil Mickelson are playing for COVID-19 relief on May 24. Full Article
hari Technology: File Sharing 技术:文件分享 By www.bbc.co.uk Published On :: 2009-06-10T15:02:21+00:00 As one music download site celebrates its 10th birthday, Take Away English reports on the music download industry. Full Article Tae Takeaway English
hari Peyton Manning roasts Tom Brady in announcing charity golf event By rssfeeds.indystar.com Published On :: Thu, 07 May 2020 19:07:03 +0000 Peyton Manning, Tom Brady, Tiger Woods and Phil Mickelson are playing for COVID-19 relief on May 24. Full Article
hari Taser officers investigated after man hurt in Haringey chase By www.bbc.co.uk Published On :: Sat, 09 May 2020 16:26:37 GMT The man sustained life-changing injuries when officers fired at him as he was jumping over a wall. Full Article
hari Pray For America Shirt (ALL Profits to Charity!) By patriotdepot.com Published On :: Thu, 02 Apr 2020 16:26:43 +0000 When Italy and NYC needed help, Samaritan's Purse stepped up, erecting field hospitals to house Corona patients in both Italy and The United States of America. Now it's our turn to step up and help out. 100% ..Price: $13.95 Full Article
hari Fin24.com | WhatsApp tightens rules on sharing to curb spread of fake virus news By www.fin24.com Published On :: Tue, 07 Apr 2020 18:46:07 +0200 WhatsApp on Tuesday placed new limits on message forwarding as part of an effort to curb the spread of misinformation about the coronavirus pandemic. Full Article
hari Audiogalaxy Returns, Choruss File Sharing Plans Falter By feedproxy.google.com Published On :: Fri, 15 Oct 2010 21:15:39 -0700 Music sharing pioneer Audiogalaxy returned this week, but not with the features its former users or its founder hoped to offer. The former Napster competitor found that, even with music-industry support, a legal file-sharing service is a long way from becoming reality. Continue reading on GigaOm.com. Tags: choruss, audiogalaxy, jimgriffin, gigaom Full Article File sharing
hari EFF: 75,000 People Sued for Porn File Sharing By feedproxy.google.com Published On :: Mon, 17 Jan 2011 22:14:15 -0800 Looks like mass lawsuits aren't just for Germans anymore: The Electronic Frontier Foundation is estimating that some 75,000 people have been sued for allegedly sharing pornographic movies in the US. This revelation comes as part of a new amicus brief (PDF) the civil liberties group just filed on behalf of 500 file sharers, asking an Illinois judge to squash a lawsuit against people accues of sharing videos from First Time Videos LLC, better known under the porn brand FTVGirls. A footnote of the amicus brief states: "As of January 14, 2011, by an informal count, well (over) 75,000 people have been sued in similar (in some cases nearly identical) complaints arising from the alleged infringement of pornographic movies. This includes 4507 individuals sued in the Northern District of Illinois alone, in nine separate lawsuits. In addition, mass copyright complaints based on non-pornographic movies have also been brought against over 13,500 people in the District of Columbia." I just wrote a story about lawsuits like these on NewTeeVee, explaining how German rights holders have filed more than half a million of such lawsuits in 2010. It looks like U.S. rights holders are well on their way to repeat these numbers, unless courts step in - which is of course exactly what the EFF wants. The group blogged on Monday: "Friday's brief is the latest of EFF's efforts to stop copyright trolls -- content owners and lawyers who team up to extract settlements from thousands of defendants at a time. Tactics include improperly lumping defendants together in one case and filing it in a court far away from most of the accused people's homes and Internet connections. When adult film companies file these predatory lawsuits, there is the added pressure of embarrassment associated with pornography. All of these factors can convince those ensnared in the suits to quickly pay what's demanded of them instead of arguing the merits of their case in court." It's worth noting that not everyone in the porn biz ready to sue the file sharing masses. Pink Visual's Allison Vivas told me recently that she has no intention to sue individuals, and Private CEO Berth Milton even said that piracy is promotion. Watch the entire interview with him embedded below: Tags: porn,, eff,, lawsuits Full Article Lawsuits
hari Busted! Late-Night Hack Comedian, Jimmy Kimmel Is Forced To Apologize For Sharing Highly Edited Video Of VP Pence To Make Him Look Bad By 100percentfedup.com Published On :: Sat, 09 May 2020 01:45:58 +0000 The following article, Busted! Late-Night Hack Comedian, Jimmy Kimmel Is Forced To Apologize For Sharing Highly Edited Video Of VP Pence To Make Him Look Bad, was first published on 100PercentFedUp.com. Last night, Jimmy Kimmel, host of the low-rated, late-night Jimmy Kimmel Show, shared a deceptively edited video clip of Vice President Pence delivering PPE to a nursing home. Today, liberal activist Matt McDermott tweeted the videotaped segment on VP Pence that was edited to make the vice president look like he was faking a delivery […] Continue reading: Busted! Late-Night Hack Comedian, Jimmy Kimmel Is Forced To Apologize For Sharing Highly Edited Video Of VP Pence To Make Him Look Bad ... Full Article Featured Politics
hari NF-{kappa}B mediates lipopolysaccharide-induced alternative pre-mRNA splicing of MyD88 in mouse macrophages [Signal Transduction] By feedproxy.google.com Published On :: 2020-05-01T00:06:09-07:00 Although a robust inflammatory response is needed to combat infection, this response must ultimately be terminated to prevent chronic inflammation. One mechanism that terminates inflammatory signaling is the production of alternative mRNA splice forms in the Toll-like receptor (TLR) signaling pathway. Whereas most genes in the TLR pathway encode positive mediators of inflammatory signaling, several, including that encoding the MyD88 signaling adaptor, also produce alternative spliced mRNA isoforms that encode dominant-negative inhibitors of the response. Production of these negatively acting alternatively spliced isoforms is induced by stimulation with the TLR4 agonist lipopolysaccharide (LPS); thus, this alternative pre-mRNA splicing represents a negative feedback loop that terminates TLR signaling and prevents chronic inflammation. In the current study, we investigated the mechanisms regulating the LPS-induced alternative pre-mRNA splicing of the MyD88 transcript in murine macrophages. We found that 1) the induction of the alternatively spliced MyD88 form is due to alternative pre-mRNA splicing and not caused by another RNA regulatory mechanism, 2) MyD88 splicing is regulated by both the MyD88- and TRIF-dependent arms of the TLR signaling pathway, 3) MyD88 splicing is regulated by the NF-κB transcription factor, and 4) NF-κB likely regulates MyD88 alternative pre-mRNA splicing per se rather than regulating splicing indirectly by altering MyD88 transcription. We conclude that alternative splicing of MyD88 may provide a sensitive mechanism that ensures robust termination of inflammation for tissue repair and restoration of normal tissue homeostasis once an infection is controlled. Full Article
hari An enzyme-based protocol for cell-free synthesis of nature-identical capsular oligosaccharides from Actinobacillus pleuropneumoniae serotype 1 [Enzymology] By feedproxy.google.com Published On :: 2020-04-24T06:08:45-07:00 Actinobacillus pleuropneumoniae (App) is the etiological agent of acute porcine pneumonia and responsible for severe economic losses worldwide. The capsule polymer of App serotype 1 (App1) consists of [4)-GlcNAc-β(1,6)-Gal-α-1-(PO4-] repeating units that are O-acetylated at O-6 of the GlcNAc. It is a major virulence factor and was used in previous studies in the successful generation of an experimental glycoconjugate vaccine. However, the application of glycoconjugate vaccines in the animal health sector is limited, presumably because of the high costs associated with harvesting the polymer from pathogen culture. Consequently, here we exploited the capsule polymerase Cps1B of App1 as an in vitro synthesis tool and an alternative for capsule polymer provision. Cps1B consists of two catalytic domains, as well as a domain rich in tetratricopeptide repeats (TPRs). We compared the elongation mechanism of Cps1B with that of a ΔTPR truncation (Cps1B-ΔTPR). Interestingly, the product profiles displayed by Cps1B suggested processive elongation of the nascent polymer, whereas Cps1B-ΔTPR appeared to work in a more distributive manner. The dispersity of the synthesized products could be reduced by generating single-action transferases and immobilizing them on individual columns, separating the two catalytic activities. Furthermore, we identified the O-acetyltransferase Cps1D of App1 and used it to modify the polymers produced by Cps1B. Two-dimensional NMR analyses of the products revealed O-acetylation levels identical to those of polymer harvested from App1 culture supernatants. In conclusion, we have established a protocol for the pathogen-free in vitro synthesis of tailored, nature-identical App1 capsule polymers. Full Article
hari CBD News: The CBD Secretariat is launching a quarterly e-Newsletter on the Ecosystem Approach, in order to facilitate sharing of information on the application of the ecosystem approach and promote the use and voluntary update of the Ecosystem Approach So By www.cbd.int Published On :: Tue, 16 Dec 2008 00:00:00 GMT Full Article
