Plazomicin was active against 97.0% of 8,783 Enterobacterales isolates collected in the United States (2016 and 2017), and only 6 isolates carried 16S rRNA methyltransferases conferring resistance to virtually all aminoglycosides. Plazomicin (89.2% to 95.9% susceptible) displayed greater activity than amikacin (72.5% to 78.6%), gentamicin (30.4% to 45.9%), and tobramycin (7.8% to 22.4%) against carbapenem-resistant and extensively drug-resistant isolates. The discrepancies among the susceptibility rates for these agents was greater when applying breakpoints generated using the same stringent contemporary methods applied to determine plazomicin breakpoints.

This study was conducted in treatment-naive adults with drug-susceptible pulmonary tuberculosis in Port-au-Prince, Haiti, to assess the safety, bactericidal activity, and pharmacokinetics of nitazoxanide (NTZ). This was a prospective phase II clinical trial in 30 adults with pulmonary tuberculosis. Twenty participants received 1 g of NTZ orally twice daily for 14 days. A control group of 10 participants received standard therapy over 14 days. The primary outcome was the change in time to culture positivity (TTP) in an automated liquid culture system. The most common adverse events seen in the NTZ group were gastrointestinal complaints and headache. The mean change in TTP in sputum over 14 days in the NTZ group was 3.2 h ± 22.6 h and was not statistically significant (P = 0.56). The mean change in TTP in the standard therapy group was significantly increased, at 134 h ± 45.2 h (P < 0.0001). The mean NTZ MIC for Mycobacterium tuberculosis isolates was 12.3 μg/ml; the mean NTZ maximum concentration (C_max) in plasma was 10.2 μg/ml. Negligible NTZ levels were measured in sputum. At the doses used, NTZ did not show bactericidal activity against M. tuberculosis. Plasma concentrations of NTZ were below the MIC, and its negligible accumulation in pulmonary sites may explain the lack of bactericidal activity. (This study has been registered at ClinicalTrials.gov under identifier NCT02684240.)

Background:

We assessed the ability to supplement existing epidemiologic/etiologic studies with data on treatment and clinical outcomes by linking to publicly available cancer registry and administrative databases.

Adeno-associated virus (AAV) recombinants are currently the vector of choice for many gene therapy applications. As experimental therapies progress to clinical trials, the need to characterize recombinant adeno-associated viruses (rAAVs) accurately and reproducibly increases. Accurate determination of rAAV infectious titer is important for determining the activity of each vector lot and for ensuring lot-to-lot consistency. The following protocol developed in our laboratory uses a 96-well TCID₅₀ format and quantitative polymerase chain reaction (qPCR) detection for the determination of rAAV infectious titer.

This protocol is used to determine the concentration of DNase-resistant vector genomes (i.e., packaged in the capsid) in purified recombinant adeno-associated virus (rAAV) preparations. The protocol begins with treatment of the vector stock with DNase I to eliminate unencapsidated AAV DNA or contaminating plasmid DNA. This is followed by a heat treatment to heat-inactivate DNase I, to disrupt the viral capsid, and to release the packaged vector genomes for quantification by real-time polymerase chain reaction (PCR) using a set of standards (linearized plasmid used for vector production) containing known copy numbers. To accomplish high-throughput titration, the primer and probe sets used in real-time PCR are usually designed to target common elements present in most rAAV genomes, such as promoters and poly(A) signals. This strategy significantly reduces the number of PCRs, controls, and turnaround time. Several important controls should be included in the assay as follows: The first two controls should have a known copy number of the rAAV genome plasmid treated or not treated with DNase I. This control tests the effectiveness of DNase treatment. To control for potential cross-contamination between samples during the preparation process, a blank control containing nuclease-free water only should be processed and tested in parallel. A validation vector sample with a known titer should be included in every assay to monitor interassay variability. Finally, for the PCR run, a no-template control (NTC) is included to indicate cross-contamination during PCR setup.

BACKGROUND AND PURPOSE:

Not all tandem occlusions diagnosed on traditional vascular imaging modalities, such as MRA, represent actual complete ICA occlusion. This study aimed to explore the utility of high-resolution vessel wall imaging in identifying true ICA tandem occlusions and screening patients for their suitability for endovascular recanalization.

BACKGROUND AND PURPOSE:

Accurate differentiation between glioblastoma and solitary brain metastasis is of vital importance clinically. This study aimed to investigate the potential value of the inflow-based vascular-space-occupancy MR imaging technique, which has no need for an exogenous contrast agent, in differentiating glioblastoma and solitary brain metastasis and to compare it with DSC MR imaging.

Three marketing experts share their unconventional means of capturing the attention of potential homeowners of today.

Prof. Jason Pomeroy talks about the rise of the digital economy and its impact on the workplace.

Many people have headaches considering landed house or condominium (condo) for their accommodation choice. By analyzing the pros and cons of each of these types, the infographic below will help you make the right choice.

WELCOME TO EPISODE 37 OF THE TALENTED LEARNING SHOW! Learn how trade associations and training companies are now pivoting to total digital education due to […]

The post Podcast 37: How to Pivot to Digital Education – With Ashish Rangnekar appeared first on e-Learning Feeds.

Piaggio India कंपनी ने Vespa 946 Emporio Armani नाम का एक स्कूटर लॉन्च किया था। इस स्कूटर को दुनिया के जानेमाने डिजाइनर Emporio Armani ने डिजाइन किया था।

Generative visuals and eerie sound design based on height maps of glaciers, canyons and estuaries. London-based motion graphic designer Sophie Marschner transforms real-world data into a delicate digital topography in Monolith. By using the height maps of various large-scale landmarks, including glaciers, canyons and estuaries, Marschner maps the outside world into a more intimate, digital […]

The post Sophie Marschner turns real-world data into a digital topography in Monolith appeared first on FACT Magazine.

As anticipated due to the COVID-19 pandemic, Windsor’s unemployment rate closed in on historic levels in April as it reached 12.9 per cent of the labour force, according to the latest numbers released Friday by Statistics Canada. There were 21,800 people in the Windsor area recorded as being unemployed in the latest StatsCan survey. But […]

The US Special Operations Command is developing a portable face-recognition system designed to identify people 1 kilometre away. It could also be used by law enforcement

A temporary coronavirus hospital ward in China is being staffed entirely by robots. Around 200 patients with mild symptoms will be fed and treated by machines, while medical staff issue commands from outside

The Queen gave a 75th anniversary VE Day speech Friday night that doubled as a rallying cry against coronavirus. “Never give up, never despair, that was the message of VE Day,” she told the British people.

Second World War heroes would “recognize and admire,” the sacrifices the British were making today in the fight against coronavirus, the Queen added. It was her second major coronavirus-themed speech to the nation.

“It may seem hard that we cannot mark this special anniversary as we would wish. Instead we remember from our homes and our doorsteps. But our streets are not empty,” the Queen said, “They are filled with the love and the care that we have for each other.”

Read more at The Daily Beast.

A neutron star has produced gravitational waves after colliding with an unknown object – it could be the smallest black hole or biggest neutron star ever found

The TESS space telescope has found its first Earth-sized planet with conditions that might be right for life, orbiting a small star 100 light years away

Beam now belongs to a Danish robot venture factory

A military exercise in Australia demonstrates how small drones can airdrop critical medical supplies to soldiers in combat

Final Fantasy XI announces its plans for its golden years. Fossil Fighters still exists. Then Phil either recommends or doesn’t recommend Avernum. I’m still not...

A new research study, investigating how service robots in hotels could help redefine leadership and boost the hospitality industry, has taken on new significance in the light of the seismic impact of the Covid-19 outbreak on tourism and hospitality.

Parents should not feel pressured to make their young children undertake structured learning or achieve specific tasks, particularly during lockdown. A new study of children under the age of two has found that parents who take a more flexible approach to their child's learning can - for children who were easy babies - minimize behavioral problems during toddlerhood.

Jihadists burst into the gold mine where Moussa Tambura worked in Burkina Faso, forbidding everyone from smoking and drinking. “They attacked the site, killed people and burned houses,” said Tambura, 29, clenching his fists. Jihadists linked to al-Qaida and the Islamic State organization have been overrunning gold mines like Tambura’s one by one as they try to gain control of Burkina Faso’s most lucrative industry.

To campaigners’ dismay, the UK’s biggest hubs, Heathrow and Gatwick, are pushing on with plans to increase capacity

Christine Taylor has lived her entire life in the shadow of London’s Heathrow airport, her childhood bedroom affording a view of one of its two runways. She grew up in Sipson, a village that can trace its history back more than 1,000 years, but now sits immediately north of Britain’s busiest airline hub.

Now living a mile to the east in Harlington, Taylor, 62, is experiencing a rare moment of quiet, thanks to the dramatic reduction in air traffic caused by the coronavirus pandemic.

Screenshots showing repugnant and insulting remarks — some misogynistic, some racist, others hinting at drug use and sexual conquests — from a private group chat between several hockey players, including ...

The number of deaths from the coronavirus in Mexico's best-known border city, Tijuana, has soared and the COVID-19 mortality rate is twice the national average, the health ministry says, after medical staff quickly fell ill as the outbreak rampaged through hospital wards.

A dialogue on sex, consent, ownership over bodies must be introduced from a young age so that it becomes part of a dominant narrative, say experts.

New York City is the epicentre of the coronavirus outbreak in the US and hospitals are already struggling to treat everyone with covid-19, though the outbreak may not peak for three weeks

Healthcare systems need to prepare for the extensive physiotherapy and mental rehabilitation that people put on ventilators for covid-19 will need as they recover

Just over 300 iPads, with fully paid data plans, will be going out to Saskatchewan Health Authority long term care homes to help residents stay in touch with their families.

The United States international, who is now on the books at Chelsea, was given his break at Borussia Dortmund by the current Liverpool manager

The Roses are Red Challenge has caught the internet and has become a fad now as the netizens are coming up with a hilarious line which rhyme with roses are red. It is the netizens who have started this trend amid lockdown and most of them are making their own versions of their poems. Most of them tickle your funny bone with the one-liners which have caught the attention of the Internet users.

With Italy's gym clubs and training facilities having fallen silent because of the coronavirus pandemic, 28-year-old Italian gymnast Marco Lodadio, silver medallist in the 2019 men's world artistic gymnastics championships, turned his garden into a fitness studio.

Without the sophisticated facilities that high-performance athletes normally count on, Lodadio modified a structure for a swing to train on rings.

Lodadio, whose parents had a gym where he began training when he was nine, has been stuck in lockdown for almost two months and this week has finally gotten back to his usual rigourous training regime as Italy begins gradually lifting its strict restrictions.