Single-particle electron cryo-microscopy (cryoEM) has undergone a `resolution revolution' that makes it possible to characterize megadalton (MDa) complexes at atomic resolution without crystals. To fully exploit the new opportunities in molecular microscopy, new procedures for the cloning, expression and purification of macromolecular complexes need to be explored. Macromolecular assemblies are often unstable, and invasive construct design or inadequate purification conditions and sample-preparation methods can result in disassembly or denaturation. The structure of the 2.6 MDa yeast fatty acid synthase (FAS) has been studied by electron microscopy since the 1960s. Here, a new, streamlined protocol for the rapid production of purified yeast FAS for structure determination by high-resolution cryoEM is reported. Together with a companion protocol for preparing cryoEM specimens on a hydrophilized graphene layer, the new protocol yielded a 3.1 Å resolution map of yeast FAS from 15 000 automatically picked particles within a day. The high map quality enabled a complete atomic model of an intact fungal FAS to be built.

Model quality assessment programs estimate the quality of protein models and can be used to estimate local error in protein models. ProQ3D is the most recent and most accurate version of our software. Here, it is demonstrated that it is possible to use local error estimates to substantially increase the quality of the models for molecular replacement (MR). Adjusting the B factors using ProQ3D improved the log-likelihood gain (LLG) score by over 50% on average, resulting in significantly more successful models in MR compared with not using error estimates. On a data set of 431 homology models to address difficult MR targets, models with error estimates from ProQ3D received an LLG of >50 for almost half of the models 209/431 (48.5%), compared with 175/431 (40.6%) for the previous version, ProQ2, and only 74/431 (17.2%) for models with no error estimates, clearly demonstrating the added value of using error estimates to enable MR for more targets. ProQ3D is available from http://proq3.bioinfo.se/ both as a server and as a standalone download.

A groundbreaking study by Harvard University’s Harvard Forest and the Smithsonian Conservation Biology Institute reveals that, if left unchecked, recent trends in the loss of […]

The post Sprawl threatens water quality, climate protection, and land conservation in Massachusetts appeared first on Smithsonian Insider.

Out in Montana’s Big Sky Country Dale Greenwalt spends his summers seeking the incredibly tiny and fragile. Armed with a putty knife honed razor-sharp on […]

The post Quality of insect fossils from Montana’s Flathead River astounds scientists appeared first on Smithsonian Insider.

The nations health care industry has foundered in its ability to provide safe, high-quality care consistently to all Americans, says a new report from the Institute of Medicine of the National Academies. Reorganization and reform are urgently needed to fix what is now a disjointed and inefficient system.

To ensure that minority students who are poorly prepared for school are not assigned to special education for that reason, educators should be required to first provide them with high-quality instruction and social support in a general education classroom before making a determination that special education is needed.

Racial and ethnic minorities tend to receive lower-quality health care than whites do, even when insurance status, income, age, and severity of conditions are comparable.

The Institute of Medicine today presented the 2012 Gustav O. Lienhard Award to Donald M. Berwick, a physician whose work has catalyzed a national movement to improve health care quality and safety.

The analysis used by federal agencies to set standards for fuel economy and greenhouse gas emissions for new U.S. light-duty vehicles -- passenger cars and light trucks -- from 2017 to 2025 was thorough and of high caliber overall, says a new report from the National Research Council.

The number of patients in the U.S. awaiting organ transplantation outpaces the amount of transplants performed in the U.S., and many donated organs are not transplanted each year due to several factors, such as poor organ function, says a new report from the National Academies of Sciences, Engineering, and Medicine.

Monitoring the quality and impact of undergraduate science, technology, engineering, and mathematics (STEM) education will require the collection of new national data on changing student demographics, instructors’ use of evidence-based teaching approaches, student transfer patterns, and other dimensions of STEM education, says a new report from the National Academies of Sciences, Engineering, and Medicine.

While the U.S. Department of Veterans Affairs (VA) provides mental health care of comparable or superior quality to care provided in private and non-VA public sectors, accessibility and quality of services vary across the VA health system, leaving a substantial unmet need for mental health services among veterans of the recent wars in Afghanistan and Iraq, says a new congressionally mandated report from the National Academies of Sciences, Engineering, and Medicine.

While legal abortions in the U.S. are safe, the likelihood that women will receive the type of abortion services that best meet their needs varies considerably depending on where they live, says a new report from the National Academies of Sciences, Engineering, and Medicine.

The U.S. Census Bureau should develop a detailed concept and implementation plan for an Annual Business Survey System (ABSS) to replace the current suite of largely separate annual economic surveys, says a new report by the National Academies of Sciences, Engineering, and Medicine.

Recent gains against the burden of illness, injury, and disability and commitment to universal health coverage (UHC) are insufficient to close the enormous gaps that remain between what is achievable in human health and where global health stands today, says a new report from the National Academies of Sciences, Engineering, and Medicine.

Between one-third and one-half of U.S. clinicians experience burnout and addressing the epidemic requires systemic changes by health care organizations, educational institutions, and all levels of government, says a new report from the National Academy of Medicine.

A new report from the National Academies of Sciences, Engineering, and Medicine finds that there is no risk-free setting for giving birth, whether at home, in a birth center, or in a hospital.

Global air quality will significantly deteriorate by 2050 unless further steps are taken to cut current emissions from human activities, according to recent research. Most people around the world will be affected by worsening air quality with hotspots of particularly poor air occurring in China, northern India and the Middle East.

Staff in buildings that have been certified as ‘green’ under the LEED scheme are just as satisfied with their indoor working environment as people working in non-LEED buildings, according to research on commercial buildings. The study suggests that investment in the thermal and acoustic aspects of buildings, however, would further improve occupant satisfaction.

The impact of air quality on public welfare is important to policy development. However, it is difficult to make a clear link between the two when air pollution tends to be reported at a country level and wellbeing is an individual measure. A new study takes a step further towards linking the two by analysing regional level air quality across the EU and relating it to levels of life satisfaction.

Harbour sediments should be tested for their toxic effects on living organisms in addition to being subject to the chemical, physical and biological tests usually conducted to assess water quality, according to recent research from Portugal. This would provide a better environmental assessment of dredged material to help guide port authorities’ decisions on its disposa

A new Cochrane Review, published in the Cochrane Library today and presented at the ERS International Congress, has found evidence from randomised trials, that taking an oral vitamin D supplement in addition to standard asthma medication is likely to reduce severe asthma attacks.

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Recent research in Italy has suggested that soil quality deteriorates over time when intensively farmed under plastic tunnels. The tunnels are an obstacle to natural rainfall and artificial irrigation increases soil salinity and, as a consequence, agricultural yields can be significantly reduced.

Although soil quality is best assessed using a wide range of indicators, a smaller set may be more practical and still provide the necessary information needed to choose between land management systems. This is the conclusion of a new study in Brazil that evaluated three different indexes of soil quality based on three sets of indicators.

Restoration of upland bog habitats by blocking drainage channels has caused concern among some sheep farmers that this will reduce the quality of grazing areas. However, UK researchers have shown that drainage does not encourage growth of plants favoured by sheep, nor do sheep use drained areas more. Therefore they conclude such restoration measures are unlikely to detrimentally affect sheep grazing.

Adding compost, manure, and other forms of organic matter to farmland soil can boost earthworm numbers, crop yield, and the stability of soil, finds a recent analysis of long-term case studies. No-tillage and crop-rotation practices also have positive effects on soil, although no-tillage's benefits for earthworms are often absent on farms that use herbicides and other pesticides. The study also confirms that organic farming typically produces lower crop yields than conventional farming, but provides recommendations on how to reduce this ‘yield gap’, while highlighting positive aspects of organic agriculture.

The impact of air quality on public welfare is important to policy development. However, it is difficult to make a clear link between the two when air pollution tends to be reported at a country level and wellbeing is an individual measure. A new study takes a step further towards linking the two by analysing regional level air quality across the EU and relating it to levels of life satisfaction.

New areas of land suitable for agriculture will open up under climate change’s effects, new research predicts, particularly in far northern regions of the world. However, the overall quality of land for farming will decline and many regions, including Europe, could lose large areas of suitable land.

European policies have eliminated the most visible and harmful effects of air pollution, but current rates of emissions still pose a threat to the environment and to human health. A new study has assessed the policy scope to make further environmental improvements by applying the GAINS (Greenhouse gas-Air pollution Interactions and Synergies) model.

Valuations of water quality as an ecosystem service often fail to include related services like recreation or human health, and do not consider the effects of water quality changes due to management. Researchers have now developed a template for valuation that considers multiple services and links management actions to changes in water quality and ultimate economic value.

Bee pollination improves the shape, weight and shelf-life of strawberries, contributing a staggering €1.05 billion to the European strawberry market per year, new research suggests. By blocking bees from a set of plants, the researchers demonstrated the substantial effects of bee pollination on the quality of the fruit.

A new study from Belgium has gathered community views of brownfield regeneration. Results indicate that the often overlooked aspect of landscape quality, such as green spaces, visually-attractive areas and cultural heritage, is important in people’s opinions of brownfield regeneration schemes.

New research reveals how scientists can monitor water quality in European lakes using sensors mounted on satellites. The technique is a promising and cost-effective new tool to classify and monitor the ecological status of lakes more reliably under the Water Framework Directive (WFD).

A recent study has found that satellite images can be used to map phytoplankton blooms to aid assessment of the quality of coastal and sea waters. This method can help EU Member States monitor water under the requirements of the Water Framework Directive and the Marine Strategy Framework Directive.

Valuations of water quality as an ecosystem service often fail to include related services like recreation or human health, and do not consider the effects of water quality changes due to management. Researchers have now developed a template for valuation that considers multiple services and links management actions to changes in water quality and ultimate economic value.

The impact of shale gas development on surface water quality has been explored in a recent study. Focusing on the Pennsylvania portion of the Marcellus Shale formation (which stretches from West Virginia to the Canadian border), the researchers conclude that shale gas wells and the treatment of shale gas extraction waste have measurable impacts on downstream surface water quality.

Climate change adaptation measures could improve the security of some groups of society at the expense of others, a new study concludes. Climate change adaptation policies should be based on genuine democracy and investment must be fairly distributed, to ensure that all at-risk groups benefit equally, its authors recommend.

Changing agricultural management practices can significantly improve water quality, according to a long-term study. The researchers found that in a US lake the total amount of suspended sediment fell, and water clarity increased as a result of multiple integrated practices implemented to reduce runoff in the surrounding area. These included introducing buffer strips of vegetation and planting trees.

Agricultural run-off can contain pesticides, sediment particles and nitrates and is a major threat to the health of the sea. Although there are policy frameworks to reduce run-off water, they often don’t clearly explain how to maximise benefits. A new study provides an economic framework that prioritises methods based on their cost-effectiveness, which could help policymakers to reduce the pollution of marine ecosystems.

Water policies at European and French national levels have led to a clear improvement in the water quality of the River Seine, a new study has found. A significant reduction in phosphate and ammonium pollution and increasing oxygen concentrations are evident. However, nitrate concentrations are still higher than the recommended level for good freshwater status, despite substantial reductions of surplus nitrogen in agricultural soils over the past few decades. The researchers recommend strengthening current agri-environmental management measures to help the river to return to a fully healthy status.

Worldwide, programmes have been implemented to protect water quality from human pressures, often using ecological indicators as a method of evaluation. An eight-year study of a Portuguese estuary has found that indicators based on multiple measures of fish communities, such as the number and relative abundance of resident and migrant species, reflect human pressures on these transitional waters and could improve the implementation of water protection programmes.

A new study suggests that improving the quality of existing wildlife habitat is more important than creating new habitat, when attempting to counteract the negative effects of climate change on species survival. This suggests that resources would be better directed towards habitat rehabilitation and reforestation than building new habitat features.

Urban waterways can provide foraging opportunities for a range of bat species. However researchers have found that bats in the UK are negatively affected by high levels of invasive plant species and urban development near waterways. The researchers highlight the value these often disregarded urban spaces can have for ecosystems, and suggest ways to improve the biodiversity of waterways.

The warmer, drier conditions expected under on-going climate change will reduce the rates at which soil fauna and microbes decompose plant litter, suggests new research from Germany. This may have important implications for agriculture and natural ecosystems worldwide, as litter decomposition is a key process in cycling and distributing nutrients throughout ecosystems.

The European eel could act as an indicator of the ecological quality of aquatic environments, according to a new study. The research suggests that new pollution limits could be developed based on levels of pollutants in eel muscle, with the aim of improving the ecological quality of water under the Water Framework Directive (WFD).

Soil biodiversity, soil quality, and soil health are integral to protecting the natural environment. Soils are crucial to food production and human well-being, as highlighted by the UN Sustainable Development Goals (SDGs). The abundance of soil biota is of great importance for the provision of associated ecosystem services (ES) and fundamental driver of self-regulation in soil. This study explores how the presence, or absence, of earthworms affects aspects of crop health and productivity, focusing on their shielding of winter wheat from the toxic plant fungi Fusarium.

If the effects of air pollution on climate change are included in air quality strategies, efficiency gains of €2.5 billion can be achieved in the EU, according to recent research.

New research suggests potential benefits in integrating air quality and climate change policy. It predicts that accounting for the climate impact of certain air pollutants in the EU, USA and China could complement policies designed to reduce the air quality impacts of these pollutants.