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Three distinct glycosylation pathways are involved in the decoration of Lactococcus lactis cell wall glycopolymers [Microbiology]

Extracytoplasmic sugar decoration of glycopolymer components of the bacterial cell wall contributes to their structural diversity. Typically, the molecular mechanism that underpins such a decoration process involves a three-component glycosylation system (TGS) represented by an undecaprenyl-phosphate (Und-P) sugar-activating glycosyltransferase (Und-P GT), a flippase, and a polytopic glycosyltransferase (PolM GT) dedicated to attaching sugar residues to a specific glycopolymer. Here, using bioinformatic analyses, CRISPR-assisted recombineering, structural analysis of cell wall–associated polysaccharides (CWPS) through MALDI-TOF MS and methylation analysis, we report on three such systems in the bacterium Lactococcus lactis. On the basis of sequence similarities, we first identified three gene pairs, csdAB, csdCD, and csdEF, each encoding an Und-P GT and a PolM GT, as potential TGS component candidates. Our experimental results show that csdAB and csdCD are involved in Glc side-chain addition on the CWPS components rhamnan and polysaccharide pellicle (PSP), respectively, whereas csdEF plays a role in galactosylation of lipoteichoic acid (LTA). We also identified a potential flippase encoded in the L. lactis genome (llnz_02975, cflA) and confirmed that it participates in the glycosylation of the three cell wall glycopolymers rhamnan, PSP, and LTA, thus indicating that its function is shared by the three TGSs. Finally, we observed that glucosylation of both rhamnan and PSP can increase resistance to bacteriophage predation and that LTA galactosylation alters L. lactis resistance to bacteriocin.




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Episode 15: The Perks of Being a Wallflower/Beasts of the Southern Wild

  • The Perks of Being A Wallflower Review
  • Beasts of the Southern Wild Review
  • What We Watched
Download Episode 15 here. (right click to save)

Next episode is our Caveman episode. We will be discussing Quest For Fire, 10,000 B.C. and Ice Man.





wall

Three distinct glycosylation pathways are involved in the decoration of Lactococcus lactis cell wall glycopolymers [Microbiology]

Extracytoplasmic sugar decoration of glycopolymer components of the bacterial cell wall contributes to their structural diversity. Typically, the molecular mechanism that underpins such a decoration process involves a three-component glycosylation system (TGS) represented by an undecaprenyl-phosphate (Und-P) sugar-activating glycosyltransferase (Und-P GT), a flippase, and a polytopic glycosyltransferase (PolM GT) dedicated to attaching sugar residues to a specific glycopolymer. Here, using bioinformatic analyses, CRISPR-assisted recombineering, structural analysis of cell wall–associated polysaccharides (CWPS) through MALDI-TOF MS and methylation analysis, we report on three such systems in the bacterium Lactococcus lactis. On the basis of sequence similarities, we first identified three gene pairs, csdAB, csdCD, and csdEF, each encoding an Und-P GT and a PolM GT, as potential TGS component candidates. Our experimental results show that csdAB and csdCD are involved in Glc side-chain addition on the CWPS components rhamnan and polysaccharide pellicle (PSP), respectively, whereas csdEF plays a role in galactosylation of lipoteichoic acid (LTA). We also identified a potential flippase encoded in the L. lactis genome (llnz_02975, cflA) and confirmed that it participates in the glycosylation of the three cell wall glycopolymers rhamnan, PSP, and LTA, thus indicating that its function is shared by the three TGSs. Finally, we observed that glucosylation of both rhamnan and PSP can increase resistance to bacteriophage predation and that LTA galactosylation alters L. lactis resistance to bacteriocin.




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Evidence of Late Pleistocene human colonization of isolated islands beyond Wallace's Line

(Max Planck Institute for the Science of Human History) What makes our species unique compared to other hominins? High profile genetic, fossil and material culture discoveries present scientists working in the Late Pleistocene with an ever-more complex picture of interactions between early hominin populations. One distinctive characteristic of Homo sapiens, however, appears to be its global distribution. Exploring how Homo sapiens colonized most of the world's continents in a relatively short period could reveal the exceptional capacities of humans relative to other hominins.




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Building blocks of the cell wall: pectin drives reproductive development in rice

(University of Tsukuba) Researchers from the University of Tsukuba have revealed that pectin, a carbohydrate found in plant cell walls, plays a vital part in the development of female reproductive tissues of rice plants. It was found that the presence of a gene involved in pectin modification increased plant fertility relative to a modified plant with the gene removed. These findings could have major implications in crop variety development and genetic modification.




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XG Firewall is now available on AWS

We are extremely pleased to announce the availability of XG Firewall on Amazon Web Services (AWS) public cloud infrastructure.





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One of two men murdered in Swallowfield identified

One of the two men killed in Swallowfield, along Old Hope Road in St Andrew, yesterday has now been identified.  The police say he is 40 year-old Clinton Jarrett, a welder of Cambridge district, St. Andrew. The other man is yet...




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Why We Build Walls: 30 Years After the Fall of the Berlin Wall

8 November 2019

Robin Niblett

Director and Chief Executive, Chatham House

Gitika Bhardwaj

Editor, Communications & Publishing, Chatham House
Robin Niblett talks to Gitika Bhardwaj about the physical and psychological significance of border walls and their role in politics today.

GettyImages-1184642325.jpg

Part of the Berlin Wall still standing today. 9 November marks the 30th anniversary of the fall of the Berlin Wall that soon led to the collapse of the communist East German government. Photo: Getty Images.

This year marks the 30th anniversary of the fall of the Berlin Wall. The wall, which stood between 1961 to 1989, came to symbolize the ‘Iron Curtain’ – the ideological split between East and West – that existed across Europe and between the two superpowers, the US and the Soviet Union, and their allies, during the Cold War. How significant was the Berlin Wall during the Cold War – was it more important physically or psychologically?

The Berlin Wall was important physically, as well as psychologically, because Berlin was the only city that was divided physically by the Cold War between the Soviet Union and its allies in the Eastern Bloc and the West.

Given the disparity that quickly emerged between the two sides in economic wealth, freedom of expression and so on, the fear was that, without that wall, there would've been a unification of Berlin in a way that the Soviet side would have lost.

But it was also very important psychologically because it became the symbol of the division between two ideologies that saw each other as inimical to each other.

That meant that if you wanted to visualize the Cold War, and the separation between the capitalist, democratic system of the West and the communist, command-and-control system of the East, Berlin offered a place where you could physically walk from one world, through a checkpoint, into the other. The whole Cold War could be reduced to this one nexus point.

Because of its psychological as well as its physical significance, the fall of the Berlin Wall quickly became the symbol of the collapse of the communist ideology it had shielded.

Since the fall of the Berlin Wall, European countries have reportedly built over 1,000 kilometres of walls – the equivalent of more than six times the total length of the Berlin Wall – along their borders.

Why has Europe been building more walls and how effective have they been? Have they been used more as symbols to appeal to political bases, and if so, has it worked with voters?

The walls that have been built in Europe recently have been for a very specific reason. This was the huge influx of migrants and refugees to Europe in 2015, through what was called the ‘eastern Mediterranean’ or ‘western Balkan route’, from Turkey to Greece and on through the Balkans, Serbia and Hungary to northern Europe – in what was Europe's biggest migrant and refugee crisis since the Second World War.

What’s interesting is that for Viktor Orbán and the Hungarian government, which was on the frontline of the flow of migrants and refugees, building a wall was a way of reasserting its sovereignty. 

Like many other countries along the ‘migrant route’, they resented that the rules under which people could migrate into Europe were flouted by northern European governments which were willing to accept large numbers of migrants and refugees.

By accepting them, they kept attracting more, and so Orbán was worried that, at some point, Germany might say ‘We can’t take anymore’ and they’d be left in Hungary.

It’s important to remember that the communist states of central and eastern Europe were kept in aspic by the Soviet Union – they existed in a hermetically sealed environment without immigration. As a result, they didn’t experience the rise of multicultural societies of the sort that emerged in Britain, Belgium, France and Germany, where immigration persisted throughout the Cold War period.

The countries of central and eastern Europe were delighted that the Berlin Wall collapsed because it allowed them to unify with western Europe. They had been vassal states of the Soviet Union during the Cold War, and by joining the EU, they re-discovered personal freedom and re-gained national sovereignty. They thought they had become masters of their own future again.

But they suddenly found they were on the frontline of a new movement of people that wanted to get into the same world that they’d entered some 15 years earlier. And, as hundreds of thousands of migrants and refugees began arriving, they suddenly realised they were in a union that did not respect their sovereignty.

So, for them, putting up walls was a sovereign act against a European Union that didn’t seem to take their sovereignty seriously.

Has it worked? Definitely. The flow of migrants has been reduced drastically. This is partly because the EU paid Turkey to hold back the over three million migrants based there. But the walls also acted as a physical and psychological deterrent. 

It also worked politically. It allowed Viktor Orbán and other European parties that took the sovereigntist line to strengthen their appeal to voters – voters like to know that governments can do certain things like protecting them and their borders.

What is hypocritical, however, is that many of the governments in western Europe which criticized the Hungarian government for building its wall have actually been rather grateful that they did so as it slowed down the flow of migrants to their countries.

Then there’s the additional hypocrisy of the EU criticizing Donald Trump for building his wall with Mexico when Europeans are benefitting from theirs in Hungary.

Two years before the fall of the Berlin Wall, former US president Ronald Reagan challenged Soviet leader Mikhail Gorbachev to ‘tear down this wall’ declaring ‘across Europe this wall will fall. For it cannot withstand [freedom].’

32 years later, building a wall along the US–Mexico border has become a cornerstone of the current US administration under Donald Trump who has pledged to build a ‘big beautiful wall’. How does this reflect the political evolution of the US and what effect does that have across the rest of the world?

President Reagan talked about tearing down the Berlin Wall as a symbol of the Cold War. He knew that the fall of the wall would undermine the Soviet Union. 

President Trump is way beyond the Cold War. Building a new wall is his response to the growing sense of economic dislocation that segments of America, like Britain and other parts of Europe and the developed world, have experienced on the back of the rise of globalization, which was partly the result of the end of the Cold War but also the rise of China.

The spread of globalization, the declining earning power of many workers in the West, advances in technology which have taken away many high-earning jobs, the eight years of austerity after the global financial crisis – these are all factors driving Trump’s thinking. 

Have inflows of Mexican immigrants or immigrants through the Mexico border been the principal driver of economic insecurity?  No.  What you’ve got is Trump promising to build a wall as a symbol of his administration’s determination to protect Americans.

So I’d say the US–Mexico wall is another symbolic – or psychological – wall. Trump’s wall is supposedly about stopping illegal immigration but there are still plenty of ways to come through the border posts. It’s principally an exercise in political theatre.

Construction site for a secondary border fence, following the length of the current primary border fence, separating the US and Mexico in San Diego. Photo: Getty Images.

From the Great Wall of China to Hadrian’s Wall, walls and fences of all sorts have been used throughout history for defence and security, but not all of them have been physical.

So-called ‘maritime walls’, as well as ‘virtual walls’, are also increasingly being enforced which, today, includes border forces patrolling seas and oceans, such as in the Mediterranean Sea or off the coasts of Australia, and border control systems controlling the movement of people. Politically how do these types of barriers compare to physical ones?

You could argue that the Mediterranean Sea, and the European border forces operating within it, still act as a physical wall because they constitute a physical obstacle to migrants being able to move from the South across the Mediterranean Sea into Europe. 

So I don’t see this maritime wall being much different to the physical walls that have been built to try to stop migrants – just like any other border patrol, the Italian navy is preventing NGO vessels carrying migrants, who have been stranded at sea from docking at Italian ports. 

In this sense, you could argue that the Mediterranean Sea is a larger version of the Rio Grande between the US and Mexico which also incorporates physical barriers along its shores.

I think the more interesting walls that are being built today are virtual walls such as regulatory walls to trade, or with the internet, new barriers are being built to digital communication which affect your capacity to access information. 

In the end, all these walls are manifestations of national sovereignty through which a government demonstrates it can ‘protect’ its citizens – whether they are successful in this objective or not.

The border between Ireland and Northern Ireland, and the presence of enforcement mechanisms along the border, has become a key issue in the Brexit negotiations. How much of the debate over this is about the symbolism of the border against its economic implications?

The Irish border carries great symbolic importance because it reflects the reality of the separation of two sovereign states.

On the island of Ireland, the British and Irish governments have wanted to minimize this reality to the greatest extent possible. They even went as far as removing all types of barriers as part of the Good Friday Agreement.

This is the same sort of fiction the European Union created when it removed any physical manifestations of the existence of borders between those member states in its Schengen agreement on borderless travel.

By removing physical manifestations of the border, the UK was able to reduce some of the popular support for Irish unification as well as support for the IRA’s campaign of violence and terrorism to try to force the same outcome. 

Brexit has thrown a huge spanner into this arrangement. If Brexit is going to mean the entire UK not being in the EU’s customs union then some sort of border would need to be reinstated.

The British government proposed to do all the checks behind the border somewhere. The EU’s view was, ‘Well, that’s nice for you to say, but this border will become the EU’s only land border with the UK, and you cannot guarantee that people won’t be able to smuggle things through.’

On the other hand, recreating a border of some sort, whether physical or not, would reignite the differentiation between the two nations – running counter to the spirit of the Good Friday Agreement.

The only solution available to Prime Minister Boris Johnson has been to put the border down the Irish Sea.  While this means that Northern Ireland will no longer be an obstacle to the UK signing new, post-Brexit, free trade agreements with other countries, it has betrayed the Conservative Party’s unionist allies, for whom it’s essential that the UK’s borders include and not exclude them. 

By the end of the Cold War there were just 15 walls and fences along borders around the world, but today, there are at least 70. How effective, do you think, building barriers are as a political and military strategy to defence and security issues given their financial – and human – cost?

Physical barriers can be an effective form of protection – or imprisonment. 

The separation wall between Israel and the Palestinian territories has reduced the level of terrorist violence being perpetrated in Israel, but the cost has been the impoverishment of many Palestinians, and is another nail in the coffin of a two-state solution.

Yet many Israelis are saying that, maybe, being entirely separate is the best way to achieve peace between the two sides.

However, the walls around the Gaza Strip have not prevented, for various reasons, the Hamas government from developing rockets and firing them into Israel.   

You could argue that the border between China and North Korea, which is severely patrolled, has been a tool of continued political control protecting the Kim Jong-un regime from collapse – as has its virtual border preventing internet penetration.

Similarly, the virtual border the Chinese government has created around its own internet, the ‘great firewall’, has been very effective both economically – allowing Chinese internet platforms to develop without the threat of competition – and also as a form of political control that helps the Chinese Communist Party retain its monopoly on power. 

So walls in all of their shapes and forms can work. They are like sanctions – sanctions are easy to impose but difficult to remove. Walls are easy to build but they’re difficult to break down. 

But my view would be that they still only work temporarily. In the end, walls serve their particular purpose for a particular period, like the Berlin Wall, they end up outliving their purpose.

You have to be alive to the fact that, whether that purpose was a good or bad purpose, there will be a moment when walls end up protecting the interests of an ever-narrower number of people inside the wall, while they cease serving, if they ever did, the interests of the growing number on both sides. 

It’s ironic that the fall of the Berlin Wall in November 1989 was not the main marker of the end of the Cold War. It began earlier that year, with the intensification of people protesting in Poland, Hungary and Czechoslovakia.

Once Hungarian troops dismantled the fence separating them from Austria in May 1989, thousands of Hungarian citizens simply walked out of their country, because by then, the wall between the East and West only existed in their minds.

Then, once East Germans also realized that Mikhail Gorbachev and the Soviet regime had lost its willingness to defend the Berlin Wall, it collapsed. 

So it is interesting that we’re marking the end of the Cold War with this anniversary of the fall of the Berlin Wall, which of course, did divide two halves of one country, making its fall all the more poignant and powerful. But the end of the Cold War really began with the fall of the invisible wall in people’s minds.




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A Wall Cannot Fix Problems at Border; Smart Solutions for Asylum Crisis Can

What President Trump calls a border crisis is in fact a crisis in the asylum system—one worsened at every turn by his administration’s harsh policies and rhetoric. Rather than spend $5.7 billion on a wall, it would be far more effective to use the money to retool an overwhelmed asylum system, adapt outmatched border enforcement infrastructure to respond to the changing composition of arrivals, and work cooperatively with Mexico to tackle the factors propelling Central Americans to flee.




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Wallaby found wandering British countryside after zoo escape

Police in Britain said a surprised jogger tipped officers off to the location of a wallaby that escaped from a zoo and went hopping through the countryside.




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Look: Fox rescued from between walls of home, garage

Animal rescuers in Britain said a fox was rescued from the narrow gap between the external walls of a house and its garage.




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Beyond Walls and Tariffs: Responding to Migration Challenges at the U.S.-Mexico Border

This event organized by the Migration Policy Institute and American Enterprise Institute features a conversation on U.S.-Mexico border conditions, as well as policy responses and regional cooperation on illegal immigration. 




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Beyond Walls and Tariffs: Responding to Migration Challenges at the U.S.-Mexico Border

This event features a smart conversation by a range of experts on U.S.-Mexico border conditions, looking at policy responses by both countries and regional cooperation.




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U.S. stocks rise again on Wall Street despite job losses

The United States' bellwether stock index, the Dow Jones Industrial Average, climbed another 455 points Friday, posting its first weekly gain in three weeks.




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Count Today's Calories, And Check Your Wallet

What do the war in Iraq, your Christmas shopping and this week's Thanksgiving dinner have in common?




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Wondering Wall Street's Mood? Look Up

Forget about buying low and selling high. If you are worried about the recent volatility in the stock market, perhaps you should let the weather be your guide.




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How we could change the planet's climate future | David Wallace-Wells

The climate crisis is too vast and complicated to solve with a silver bullet, says author David Wallace-Wells. What we need is a shift in how we live. Follow along as he lays out some of the dramatic actions we could take to build a livable, prosperous world in the age of global warming.




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The intangible effects of walls | Alexandra Auer

More barriers exist now than at the end of World War II, says designer Alexandra Auer. And when you erect one wall, you unwittingly create a second -- an "us" versus "them" partition in the mind that compromises our collective safety. With intriguing results from her social design project focused on two elementary schools separated by a fence, Auer encourages us to dismantle our biases and regain perspective on all the things we have in common.




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Boy swallows universe / Trent Dalton.

Australian fiction.




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Barium swallow, Pressure and cardiac failure, Polar physiology.

[S.i.] : [publisher not identified]




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Diet in infancy : the essential introduction to the study of disease in childhood / by A. Dingwall-Fordyce.

Edinburgh : W. Green, 1908.




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The elastic properties of the arterial wall / by Charles S. Roy.

[Place of publication not identified] : [publisher not identified], 1880.




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Idaho Educators Who Dressed Up as the Border Wall Put on Administrative Leave

After photos surfaced of staff at dressed in Halloween costumes as Mexicans and a border wall bearing the slogan "Make America Great Again," the employees responsible won't be returning to their classrooms on Monday.




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Men building Hadrian's wall, being reprimanded by a Roman centurion. Photograph after W.B. Scott.

[19--?]




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King Charles I on horseback outside the city walls of Hull: the Parliamentarians inside, led by Sir John Hotham, refuse to surrender the city. Engraving by N. Tardieu after C. Parrocel.

London : Printed and sold by Thos. and John Bowles, printsellers, [1728]




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2020 NHL draft profile: William Wallinder looks a lot like Flyers prospect Egor Zamula

The next Egor Zamula? A package of size and skill could be there for the Flyers in the 2020 NHL draft with William Wallinder. By Jordan Hall




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Item 07: A Journal of ye [the] Proceedings of his Majesty's Sloop Swallow, Captain Phillip [Philip] Carteret Commander, Commencing ye [the] 23 of July 1766 and ended [4 July 1767]




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Item 08: A Logg [Log] Book of the proceedings on Board His Majesty's Ship Swallow, Captain Philip Carteret Commander Commencing from the 20th August 1766 and Ending [21st May 1768]




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Item 10: Log book of the Swallow from 22 August 1767 to 4 June 1768 / by Philip Carteret




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Item 13: Swallow 1767, A journal of the proceedings on Board His Majesty's Sloop Swallow, commencing the 1st of March 1767 and Ended the 7th of July 1767




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Box 3: Children's book illustrations by various artists, Peg Maltby and Dorothy Wall, , ca. 1932-1975




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Box 4: Children's book illustrations by various artists, Dorothy Wall, ca. 1932




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Box 6: Children's book illustrations by various artists, Dorothy Wall and Noela Young, ca. 1932-1964




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Schools without walls

Smiley and energetic, Christine lives in the semi-arid region of Karamoja in north-east Uganda. Her husband passed away some time ago and she is now taking care of her six children on her own. Christine struggled in managing her household and securing the basic needs for her children. “I was permanently asking somebody for something,” she describes. Agriculture had always been [...]




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#m715 Hiking & "Wall Alley"




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A Buffer Zone Around Saturn May Have Kept It From Swallowing Its Biggest Moon

A new simulation points to a previously untold chapter in Titan’s history




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Prince Edward and Wallis Simpson's Sprawling Bahamas Estate Is Up for Sale

After abdicating the British throne, Edward was appointed governor of the Bahamas, where he temporarily lived in a lavish home in Nassau




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Remnants of 13th-Century Town Walls Unearthed in Wales

Caernarfon, where the discovery was made, was key to Edward I's conquest of the Welsh




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196-Foot Section of the Berlin Wall Demolished to Make Way for Condos

Angry historians say the stretch of concrete was one of the largest remaining sections of the inner wall




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Aerial Surveys Reveal Possible Fort of Scottish Patriot William Wallace

The freedom fighter may have once used the structure to conspire against English forces




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The Roman Wall That Split Britain Into Two Parts

Hadrian’s Wall was a 73 mile barrier stretching from coast to coast, splitting the warlike north of Britain from the more docile south. It was the Roman Empire’s way of imposing peace in a hostile land




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Giant, record-class walleye caught and released near Dryden, Ont.

A man from Vermilion Bay, Ont., caught and released a fish that he says could have challenged a 70-year-old record for walleye last weekend.



  • News/Canada/Thunder Bay

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Walls broken down by love

The Bus4Life brings God's love to the marginalised in Tata, Hungary.




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Georgia Adderley keen to hold court again after weeks of battering living-room walls

WHILE almost every athlete has been affected by the global shutdown of sport, it is perhaps the old and the young who are feeling it the most.




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'Walls That Talk' Give Students Tools for Writing Independently (Video)

High school teacher Kateryna Haggerty explains how visual aids in her classroom help her English-language learner students write more confidently.




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Impaired Fetal Growth and Arterial Wall Thickening: A Randomized Trial of Omega-3 Supplementation

Impaired fetal growth is an independent risk factor for cardiovascular diseases in adulthood and is associated with arterial wall thickening, a noninvasive measure of subclinical atherosclerosis, in early childhood. No preventive strategy has been identified.

Dietary omega-3 fatty acid supplementation in early childhood prevented the association of impaired fetal growth with arterial wall thickening, suggesting that this early-life intervention may mitigate the risk of cardiovascular disease in those with impaired fetal growth. (Read the full article)




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Fin24.com | Eyeing Saudi riches, Wall Street gets back to business as usual despite Khashoggi aftermath

For a moment, Wall Street seemed to be inching away from Saudi Arabia. Now, it’s already inching back.




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The Als3 cell wall adhesin plays a critical role in human Serum amyloid A1 (SAA1)-induced cell death and aggregation in Candida albicans [Mechanisms of Resistance]

Antimicrobial peptides and proteins play critical roles in the host defense against invading pathogens. We recently discovered that recombinantly expressed human and mouse serum amyloid A1 (rhSAA1 and rmSAA1) proteins have potent antifungal activities against the major human fungal pathogen Candida albicans. At high concentrations, rhSAA1 disrupts C. albicans membrane integrity and induces rapid fungal cell death. In the current study, we find that rhSAA1 promotes cell aggregation and targets the C. albicans cell wall adhesin Als3. Inactivation of ALS3 in C. albicans leads to a striking decrease in cell aggregation and cell death upon rhSAA1 treatment, suggesting that Als3 plays a critical role in SAA1 sensing. We further demonstrate that deletion of the transcriptional regulators controlling the expression of ALS3, such as AHR1, BCR1, and EFG1 in C. albicans results in similar effects to that of the als3/als3 mutant upon rhSAA1 treatment. Global gene expression profiling indicates that rhSAA1 has a discernible impact on the expression of cell wall- and metabolism-related genes, suggesting that rhSAA1 treatment could lead to a nutrient starvation effect on C. albicans cells.