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IKEA: Partnering with Australia’s largest delivery provider allows us to enhance our accessibility

Australia Post and IKEA have today announced a new strategic partnership, entering into a three-year agreement to further expand IKEA’s delivery footprint in Australia.




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Starship Technologies: bringing autonomous food delivery to even more customers in Tallinn

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Driver dies after crashing on hurricane-damaged highway in North Carolina




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Driver jumps from overpass into Trinity River after crash on I-35 in Fort Worth, causing heavy traffic




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See it: Vehicle falls into North Carolina gorge after driver disregards I-40 closure following Helene




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Miss Universe contestant expelled from competition over ‘personal’ scandal as rumors swirl




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Beehive Mountain, Alberta and British Columbia, 82j/2 e1/2

Re-release; Norris, D K. no. 58-5, 1958, 25 pages (1 sheet), https://doi.org/10.4095/101214




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CRISPR Immune Cells Not Only Survive, They Thrive After Infusion Into Cancer Patients

CRISPR Immune Cells Not Only Survive, They Thrive After Infusion Into Cancer Patients

In the first-ever (sanctioned) investigational use of multiple edits to the human genome, a study found that cells edited in three specific ways and then removed from patients and brought back into the lab setting were able to kill cancer months after their original manufacturing and infusion.

This is the first U.S. clinical trial to test the gene editing approach in humans, and the publication of this new data today follows on the initial report last year that researchers were able to use CRISPR/Cas9 technology to successfully edit three cancer patients' immune cells. The ongoing study is a cooperative between Tmunity Therapeutics, the Parker Institute for Cancer Immunotherapy, and the University of Pennsylvania. 

Patients on this trial were treated by Edward A. Stadtmauer, MD, section chief of Hematologic Malignancies at Penn, co-lead author on the study. The approach in this study is closely related to CAR T cell therapy, in which patient immune cells are engineered to fight cancer, but it has some key differences. Just like CAR T, researchers in this study began by collecting a patient's T cells from blood. However, instead of arming these cells with a receptor against a protein such as CD19, the team first used CRISPR/Cas9 editing to remove three genes. The first two edits removed a T cell's natural receptors so they can be reprogrammed to express a synthetic T cell receptor, allowing these cells to seek out and destroy tumors. The third edit removed PD-1, a natural checkpoint that sometimes blocks T cells from doing their job. 

Once the three genes are knocked out, a fourth genetic modification was accomplished using a lentivirus to insert the cancer-specific synthetic T cell receptor, which tells the edited T cells to target an antigen called NY-ESO-1. Previously published data show these cells typically survive for less than a week, but this new analysis shows the edited cells used in this study persisted, with the longest follow up at nine months. 

Several months after the infusion, researchers drew more blood and isolated the CRISPR-edited cells for study. When brought back into the lab setting, the cells were still able to kill tumors. 

The CRISPR-edited T cells used in this study are not active on their own like CAR T cells. Instead, they require the cooperation of a molecule known as HLA-A*02:01, which is only expressed in a subset of patients. This means that patients had to be screened ahead of time to make sure they were a match for the approach. Participants who met the requirements received other clinically-indicated therapy as needed while they waited for their cells to be manufactured. Once that process was completed, all three patients received the gene-edited cells in a single infusion after a short course of chemotherapy. Analysis of blood samples revealed that all three participants had the CRISPR-edited T cells take root and thrive in the patients. While none responded to the therapy, there were no treatment-related serious adverse events. 

CRISPR technology has not previously been tested in humans in the U.S. so the research team had to move through a comprehensive and rigorous series of institutional and federal regulatory approval steps, including approval by the National Institutes of Health's Recombinant DNA Research Advisory Committee and review by the U.S. Food and Drug Administration, as well as Penn's institutional review board and institutional biosafety committee. The entire process required more than two years.

 Researchers say these new data will open the door to later stage studies to investigate and extend this approach to a broader field beyond cancer, several of which are already planned at Penn.

sb admin Thu, 02/06/2020 - 14:52
Categories




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Bernie Sanders tests positive for COVID-19 amid nationwide spike

Sen. Bernie Sanders (I-VT) revealed Thursday that he contracted COVID-19 during the Senate's holiday break amid an increase in infections nationwide.




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Home economics: The alternative to mortgages with sky-high rates

Mortgage rates are at their highest levels in 22 years and house prices are at record highs. Hardworking people cannot get on the property ladder, and retirees are struggling to sell in order to downsize. The Biden administration has done little to help alleviate the problem. This Washington Examiner series, Home Economics, will investigate how we got here, the toll on people around the country, and the alternatives people are embracing to survive the market. The last part of this four-part series focuses on the alternatives to traditional fixed-rate mortgages gaining new consideration among prospective home buyers.




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This Is the Best Place to Live in Virginia

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How parents and caregivers can evaluate the research on MERT and other potential treatments

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Jim Williams: D.C. native Lindsay Czarniak set to host coverage of Indianapolis 500

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Five Washington Nationals players will sit out opening day due to COVID-19 concerns

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Caps 2, Rangers 1 (OT): Five Observations

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Rangers 1, Caps 0: Five Observations

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Maryland teachers union representative suspended for antisemitic posts targeting local wealthy Jews

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California lawmakers revive debate over bill requiring tech platforms to pay for news

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Uber will add driverless Cruise vehicles to its fleet in 2025

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California's digital driver's licenses now work with Apple Wallet

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Facing skepticism, Elon Musk unveils prototype for driverless robotaxi

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Stay on Target: Overcoming Challenges in Precision Drug Delivery

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Gene Proximity to Nuclear Speckles Drives Efficient mRNA Splicing

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Unlocking the Metabolic Drivers of Alzheimer’s Disease

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From Marmosets to Menopause: A Primate Perspective

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Live Imaging Intracellular Parasites Reveals Changes to Host Metabolism

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What Drives the "Wet Dog Shakes" Reflex in Furry Animals?

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Study finds DC least festive in the US

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Caps 2, Rangers 1 (OT): Five Observations

1. So here we are again. Last year the Capitals and the New York Rangers were tied 2-2 in a best-of-seven Stanley Cup playoff series and Washington was moments away from securing Game 5. Instead, a late Joel Ward penalty led to a game-tying goal by Brad Richards with just seconds remaining. Madison Square Garden exploded and Washington wilted in a devastating overtime loss. The Caps survived Game 6 at home, but ultimately were dispatched in a bitter Game 7 loss in New York.




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The conservative kids are all right

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Metro budget proposal includes massive layoffs and service cuts to address $750 million deficit

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Driverless cars in California can get out of almost any ticket: Report

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'It takes away from the Native Americans': Son of Redskins logo designer denounces rebranding

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Native American organization wants to make ‘Redskins’ name great again

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Modernizing .NETpad: .NET 9 Arrives with a Few (More) Small Improvements for WPF (Premium)

I was excited to see Microsoft bring the Windows Presentation Foundation (WPF) back from the dead this past year: At Build 2024 back in May, it announced that it would continue investing in this 20-year-old technology, starting with support for Windows 11 theming that would arrive as part of .NET 9. In fact, I was so excited about this that I brought my .NETpad project back from the dead as well, and I spent much of the summer modernizing my Notepad clone with the new features. I wrote 24 articles documenting this work, but I was stymied by the half-assed nature of the improvements.

Microsoft released exactly one WPF update during the several months of .NET 9 development, and it never added any of the features I discovered were missing. And so as we headed into today's release of .NET 9, my excitement was somewhat diminished. My assumption was that we wouldn't see those missing features implemented until .NET 10, if ever.

Well, Microsoft just released .NET 9. As part of that release, it published updated documentation for WPF (and all the other .NET technologies). And to my surprise, there are some updates to WPF that address at least one of those missing features.

So let's take a look.

To add support for Windows 11 theming to a WPF project, you need to add a reference to the new Fluent theme resource dictionary in its App.xml file. It looks like so:

<Application.Resources>
    <ResourceDictionary>
      <ResourceDictionary.MergedDictionaries>
        <ResourceDictionary Source="pack://application:,,,/PresentationFramework.Fluent;component/Themes/Fluent.xaml" />
      </ResourceDictionary.MergedDictionaries>
    </ResourceDictionary>
</Application.Resources>

But with the shipping version of .NET 9, there's a second, more elegant way to add Windows 11 theming support. Now, you can access a new Application.ThemeMode property of a new styling API to toggle the app's theme mode between Light, Dark, System, and None. And that's fantastic, because it addresses one of those missing features: To date, .NETpad has adapted itself to the system theme (Light or Dark), but there was no way to let the user pick a theme mode. (For example, if the system was set to Dark and the user wanted the app to use Light mode.) With this change, I can implement that feature.

Fortunately, .NETpad is ready for this change, too: If you followed along with my work this past summer, you may remember that I implemented the user interface for switching the app theme into its settings interface, but left the UI hidden because it didn't do anything. But I always felt that Microsoft would need to implement this features, so I left the code in there. Granted, I didn't think it would happen this quickly.

The shipping version of .NET 9 also adds explicit support for the Windows 11 accent color (as configured by the user in the Settings app in Personalization > Accent color). As it is, .NETpa...

The post Modernizing .NETpad: .NET 9 Arrives with a Few (More) Small Improvements for WPF (Premium) appeared first on Thurrott.com.




ive

Vote No Initiative Measure No. 2109

Stop the Cuts to Child Care and Education  by Stranger Election Control Board

This crackpot initiative would repeal the state’s new capital gains tax and cut $2.2 billion for education, early learning services, and child care at a time when schools across the state face huge deficits. 

Aside from dramatically reducing funding for schools, passing this initiative would help restore Washington’s status as the state with the most unfair tax code for poor people, all in the service of helping our wealthiest residents dodge a tax that their accountants might mistake as a rounding error.