Health Ministry to send central teams to 10 States.

Virus strain from NIV Pune transferred.

A select list of stories to read before you start your day

Sara Ali Khan shared a beautiful picture of Amrita Singh and Rukhsana Sultana holding her newborn self

Afamin, which is a human blood plasma glycoprotein, a putative multifunctional transporter of hydrophobic molecules and a marker for metabolic syndrome, poses multiple challenges for crystallographic structure determination, both practically and in analysis of the models. Several hundred crystals were analysed, and an unusual variability in cell volume and difficulty in solving the structure despite an ∼34% sequence identity with nonglycosylated human serum albumin indicated that the molecule exhibits variable and context-sensitive packing, despite the simplified glycosylation in insect cell-expressed recombinant afamin. Controlled dehydration of the crystals was able to stabilize the orthorhombic crystal form, reducing the number of molecules in the asymmetric unit from the monoclinic form and changing the conformational state of the protein. An iterative strategy using fully automatic experiments available on MASSIF-1 was used to quickly determine the optimal protocol to achieve the phase transition, which should be readily applicable to many types of sample. The study also highlights the drawback of using a single crystallographic structure model for computational modelling purposes given that the conformational state of the binding sites and the electron density in the binding site, which is likely to result from PEGs, greatly varies between models. This also holds for the analysis of nonspecific low-affinity ligands, where often a variety of fragments with similar uncertainty can be modelled, inviting interpretative bias. As a promiscuous transporter, afamin also seems to bind gadoteridol, a magnetic resonance imaging contrast compound, in at least two sites. One pair of gadoteridol molecules is located near the human albumin Sudlow site, and a second gadoteridol molecule is located at an intermolecular site in proximity to domain IA. The data from the co-crystals support modern metrics of data quality in the context of the information that can be gleaned from data sets that would be abandoned on classical measures.

This work presents the crystal structure determination of two elusive polymorphs of furazidin, an antibacterial agent, employing a combination of crystal structure prediction (CSP) calculations and an NMR crystallography approach. Two previously uncharacterized neat crystal forms, one of which has two symmetry-independent molecules (form I), whereas the other one is a Z' = 1 polymorph (form II), crystallize in P21/c and P1 space groups, respectively, and both are built by different conformers, displaying different intermolecular interactions. It is demonstrated that the usage of either CSP or NMR crystallography alone is insufficient to successfully elucidate the above-mentioned crystal structures, especially in the case of the Z' = 2 polymorph. In addition, cases of serendipitous agreement in terms of 1H or 13C NMR data obtained for the CSP-generated crystal structures different from the ones observed in the laboratory (false-positive matches) are analyzed and described. While for the majority of analyzed crystal structures the obtained agreement with the NMR experiment is indicative of some structural features in common with the experimental structure, the mentioned serendipity observed in exceptional cases points to the necessity of caution when using an NMR crystallography approach in crystal structure determination.

Authoritative, intelligent and always accompanied by a young assistant, television’s Mr. Wizard brought science to America’s kids from the 1950s through 1980s with experiments using […]

The post Smithsonian acquires archives of Mr. Wizard, Don Herbert appeared first on Smithsonian Insider.

 Husband and wife artists, Mr. and Mrs. Ferguson, (who married at Burning Man in 2011) create detailed, tactile, often whimsical large-scale artworks, including the […]

The post Burning Man’s Mr. and Mrs. Ferguson appeared first on Smithsonian Insider.

Based on a thorough review of clinical and epidemiological studies, neither the mercury-based vaccine preservative thimerosal nor the measles-mumps-rubella (MMR) vaccine are associated with autism, says a new report from the Institute of Medicine of the National Academies.

“We did not request you to change the category of mobile phones to essential items. We again reiterate we are not asking to change the category of mobile phones,” Arvinder Khurana, National President All India Mobile Retailers Association said in a letter to Piyush Goyal.​

Sujit Bhattacherjee, Group CIO at Amri Hospitals provides insights into how he is leveraging AI and analytics for ensuring better healthcare outcomes.

A Utah bobcat named Mr. Murderbritches was captured in 2018 after he was caught eating a farmer's chickens.

A malformed version of the well-accessorized spud is raising awareness of food waste in the U.K.

Chuck Leavell, keyboardist for The Rolling Stones and the cofounder of MNN, talks to pop star Jason Mraz about his avocado farm and what it felt to be named the

To fulfill his promise to become the 'Climate Change President,' Obama will have to channel his inner Harry Truman.

The all-new Toyota Camry Hybrid is perfect for your next family road trip.

Video: Jason Mraz talks about touring green with Chuck at Farm Aid.

Experiencing a pleasant tingle when someone crinkles paper isn't as weird as you think. It's called ASMR or 'brain orgasm.' Here's why it happens.

Topics

Topics

Topics

Topics

Topics

Topics

Topics